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Citations for PerkinElmer :
451 - 500 of 1116 citations for 2 Methyl 4 piperidin 1 ylsulfonyl phenylboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated in the dark for 2 h at RT before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of FITC 485 / FITC 535 ...
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bioRxiv - Genomics 2022Quote: ... The DNA extraction was performed at Hospital Universitari Vall d’Hebron (Supplementary Table 2) from blood samples using chemagic DNA Blood400 Kit LH (Perkin Elmer). The DNA from oral mucosa swabs ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10uCi/mouse; PerkinElmer, Waltham, MA) was given to 4 hour fasted individually housed mice ...
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bioRxiv - Biochemistry 2020Quote: ... was counted using a PerkinElmer Tri-Carb 2800RT liquid scintillation counter after dissolving the filter in 2 ml of scintillation liquid (Emulsifier Scintillator Plus, PerkinElmer).
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bioRxiv - Cell Biology 2021Quote: ... buffer and 1.2 M sorbitol citrate solution, and then digested by 1.4 mg/ml 20T zymolyase (amsbio, 120491) and glusulase (PerkinElmer, NEE154001EA). Cells were spread on poly-L-lysine (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... we used initially the RNAscope Multiplex Fluorescent Assay version 1 with the fluorophores Alexa 488 and Atto 550 and in later experiments the RNAscope Multiplex Fluorescent Assay version 2 with the fluorophores Opal 570 and Opal 650 (PerkinElmer) according the manufacturer’s protocol for multiplex fluorescent in situ hybridization kit ...
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Cancer causes dysfunctional insulin signaling and glucose transport in a muscle-type specific mannerbioRxiv - Physiology 2021Quote: ... A fraction (50 μl) of the lysate was dissolved in 2 ml of β-scintillation liquid (Ultima Gold, Perkin Elmer) for measurement of 2-DG transport using [14C]mannitol to estimate extracellular space using β-scintillation counting ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA™ assay was in 20 μL reactions (requiring only 2 μL cell culture media sample) in 384-well plate (6007290, PerkinElmer) following standard two-step no-wash protocol without any modifications ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... 2-deoxyglucose (2DG) uptake in tissues in vivo were measured by the retro-orbital injection of [3H]2DG (Perkin Elmer) diluted in saline ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were acquired with an AxioObserver 2 mot plus fluorescence microscope (Zeiss, Munich, Germany) or with an Operetta high- content imaging system (PerkinElmer) at 5x magnification ...
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bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
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bioRxiv - Bioengineering 2020Quote: Bioprinted SK-N-BE(2) spheroids were prepared as described earlier in a 96-well plate (Cell-Carrier, Perkin Elmer). After incubating the plate for 3 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were incubated in the dark for 2 h at room temperature before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer), as described previously74 ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 pmole trypSL (1.25 µl from a 50µM stock was kinased with 2 µl 32PgammaATP (25 µM, 6000Ci/mmol, 150 mCi/ml, Perkin Elmer) with 10 U PNK in 10 µl with 20 U RNasin (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... TZM-bl reporter cells were seeded at a density of 2 × 104 cells/well in 96-well luminometer-compatible tissue culture plates (PerkinElmer) 24 h before infection ...
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bioRxiv - Microbiology 2023Quote: ... A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... The bioluminescent signal emitted by the disseminated tumors was measured at 7-day intervals for 2 months using the IVIS Spectrum In Vivo Imaging System (PerkinElmer). The weight of the mice was recorded before each measurement.
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bioRxiv - Biochemistry 2023Quote: ... FLAG and HA were detected using the LI-COR system and HRP-conjugated GAPDH primary antibody was incubated with blots for 2 hours at room temperature and detected with Western Lightning Plus-ECL (PerkinElmer) using a Bio-Rad Chemidoc System.
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bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 12 hr following TNFα addition using the Opera Phenix Plus High-Content Screening System (PerkinElmer), with two biological replicates (n = 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 48 hr following TNFα addition using the Opera QEHS imaging system (PerkinElmer). Nuclear RELA and PCNA intensities were measured using Nuclitrack software (Cooper et al. ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 25-45 images were acquired on an Opera Phenix high-content imaging system (objective 20x, z-stacks of 2 μm, PerkinElmer). Image analysis was performed using Harmony imaging analysis software (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase measurements were taken every other day beginning from day 2 (the day after the injection) – mice were anaesthetised (isoflurane inhalation) and subcutaneously injected with Xenolight D-luciferin substrate (PerkinElmer). Mice were imaged after 5min using the IVIS Lumina II (Caliper Life Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1-2 μg of total RNA was used to isolate poly-A RNA with NEXTFLEX Poly(A) beads 2.0 (Perkin Elmer), while libraries were prepared using the NEXTFLEX Rapid Directional RNA-seq Kit 2.0 (PerkinElmer) ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was scanned and images were acquired in real-time every 2 hours from 0 to 24 h post-treatment by Opera Phenix High Content Screening System by PerkinElmer, Inc ...
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bioRxiv - Microbiology 2024Quote: ... plates were incubated in the dark at 37°C + 5% CO2 before their fluorescent signal was measured at 2 hours post-incubation at 485/535nm using the Victor3V microplate reader (PerkinElmer). Background signal from unstimulated cells were subtracted from zymosan stimulated samples for analysis ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated with Ham’s F-10 media supplemented with 1.5 mCi/well myo[2-3H(N)] inositol (Perkin Elmer/Revvity) for 16-18 h ...
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bioRxiv - Biochemistry 2024Quote: ... Radioactivity was measured (counts per minute, CPM) using a scintillation counter (MicroBeta 2 Plate Counter) with a scintillation cocktail (OptiPhase SuperMix, PerkinElmer). Data were analyzed via nonlinear regression in GraphPad Prism v10.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D1R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 1nM [3H]SCH23390 (Perkin Elmer, USA, 85 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D1R ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D2R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 4nM [3H]raclopride (Perkin Elmer, USA, 60.1 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D2R ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µM cycloheximide and 10 µCi ml-1 Na-14C acetate (PerkinElmer: 56.6 mCi mM-1) were then added to the culture ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: [3H]-DHA (1-[4,6-propyl-3H]dihydroalprenolol, specific activity 91 Ci·mmol-1) was obtained from PerkinElmer Life and Analytical Sciences (Beaconsfield ...
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bioRxiv - Microbiology 2021Quote: ... spiked with 500 nmol 3H-leucine L-1 (Perkin Elmer, specific activity 100 Ci mmol- 1) and incubated in the dark for 4 h ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µM cycloheximide and 10 µCi mL-1 Na-14C acetate (PerkinElmer: 56.6 mCi mM-1) were then added to the culture for the 7-min pulse ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and ATPlite 1 Step (Perkin Elmer). The main instruments used were the microplate reader SpectraMax 340PC (Molecular Device ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 650 (1:1500, PerkinElmer, FP1496001KT), Opal 690 (1:1500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 690 (1:1500, PerkinElmer, FP1497001KT). Following signal amplification of the target probes ...
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bioRxiv - Cell Biology 2022Quote: ... 1:750 TSA Cy3 (#NEL704A001KT, PerkinElmer) and TSA Cy5 (#NEL75A001KT ...
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bioRxiv - Neuroscience 2021Quote: ... Opal 570 (1:1500, Perkin Elmer), and Opal 690 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μCi [14C]glycerol (Perkin Elmer) + 50 μM glycerol to stimulate glycerolipid synthesis ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μL γ-32P-ATP (PerkinElmer) and 10 U T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μM [3H]-SAM (Perkin Elmer), 1 μM full-length C ...
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bioRxiv - Biophysics 2023Quote: ... 1 μM 3H-L-Asp (PerkinElmer), and 0.5 μM valinomycin ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL γ-32P-ATP (PerkinElmer) and 10 U T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescence polarization measurements were carried out in black 96-well plates measured on a Wallac Victor 2 Plate Reader (Perkin Elmer). For all measurements peptide was held at concentrations of 50 nm (or 100nM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were dried for at least 30 mins at 95 °C to remove unfixed 14C before re-suspending the fixed 14C pellets with ultra-pure water and adding 2 mL of scintillation cocktail (Ultima Gold XR, PerkinElmer, US). Radioactivity measurements were then performed using a scintillation counter (Tri-Carb ...
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bioRxiv - Cell Biology 2021Quote: ... was monitored every 5 min for 2 h at 37°C using a fluorescent plate reader (EnSpire Multimode Plate Reader, Perkin Elmer). Samples were assayed in technical triplicates and in biological replicates as indicated in the figure legend.
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...