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Citations for CisBio :
1 - 50 of 135 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HTRF detection reagents (cAMP Dynamic 2 kit, Cisbio) were added at the end of the incubation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: FPR-mediated inhibition of cellular de novo cAMP formation was quantified by competitive immunoassays based on the time-resolved measurement of fluorescence resonance energy transfer (HTRF, Homogeneous Time-Resolved Fluorescence) using the cAMP-Gi Kit from Cisbio as previously published [37] ...
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bioRxiv - Physiology 2020Quote: ... or using a Homogeneous Time Resolved Fluorescence (HTRF) insulin kit (Cisbio, France) in a PHERAstar reader (BMG Labtech ...
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bioRxiv - Neuroscience 2024Quote: Secreted insulin was detected using a commercially available insulin detection kit (PerkinElmer/Cisbio Bioassays, Bedford, MA) based on homogenous time-resolved fluorescence resonance energy transfer (HTRF ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP was determined by homogenous time-resolved fluorescence (HTRF, cAMP Dynamic 2 kit, Cisbio).
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bioRxiv - Cell Biology 2023Quote: ... Insulin was measured by using a Homogeneous Time Resolved Fluorescence (HTRF) insulin assay kit (Cisbio) in a PHERAstar reader (BMG Labtech) ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were collected to quantify the amount of IL-1β by HTRF according to the manufacturer’s protocol for the human IL-1β detection kit (Cisbio). In brief ...
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bioRxiv - Molecular Biology 2023Quote: IP1 accumulation assay was applied for detection of the downstream Gαq signal of MCHR2 using the IP-One HTRF kit (Cisbio). AD293 cells were cultured in DMEM/ high Glucose supplemented with 10% (v/v ...
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bioRxiv - Physiology 2023Quote: Inositol 1 phosphate (IP1) accumulation in hPC-PL was measured using the HTRF IP-One Gαq Detection Kit (Cisbio). This assay enables the direct pharmacological activity of Gαq - coupled receptors ...
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bioRxiv - Bioengineering 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Physiology 2019Quote: ... Secreted and total insulin were quantified using a homogeneous time-resolved fluorescence (HTRF) insulin kit (Cisbio) in a PHERAstar reader (BMG Labtech ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Physiology 2020Quote: ... Secreted and total insulin were quantified using a Homogeneous Time Resolved Fluorescence (HTRF) insulin kit (Cisbio, France) in a PHERAstar reader (BMG Labtech ...
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bioRxiv - Cell Biology 2023Quote: ... Total insulin content was assessed using ultra-sensitive insulin homogeneous time-resolved fluorescence (HTRF) assay kits (Cisbio).
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bioRxiv - Cell Biology 2021Quote: ... Accumulation of IP1 was measured using the IP-One Gq Homogenous Time-Resolved Fluorescence (HTRF) kit from Cisbio per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... ERK phosphorylation was evaluated using a Homogenous Time-Resolved FRET (HTRF)-based Phospho-ERK (Thr202/Tyr204) cellular kit (Cisbio). Cells were lysed for 30 minutes with the lysis buffer provided and incubated for 2 hours with pERK1/2-specific antibodies conjugated with Eu3+-cryptate donor and d2 acceptor at recommended dilutions ...
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bioRxiv - Neuroscience 2021Quote: IP1 accumulation assay: Inositol monophosphate accumulation was determined using the IP-One HTRF (Homogenous Time Resolved Fluorescence) kit (Cisbio Bioassays ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed as in the primary screen and IL-1β in the cell- free supernatants was detected using a commercial mouse IL-1β homogenous time-resolved fluorescence (HTRF) kit (Cisbio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Phosporylated Ubiqutin at Ser65 levels were measured using time-resolved fluorescence resonance energy transfer (FRET) Phospo-Ubiqutin (Ser65) Cellular Kit (Cisbio 64UBIS65PEG) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 10μl of detection mixture containing Streptavidin-d2 (Cisbio 610SADLF, 1:200), PAb Anti Human IgG-Eu cryptate (Cisbio 61HFCKLA ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were stored at −20°C until the insulin concentration was determined using an Insulin Ultra-Sensitive Homogeneous Time Resolved Fluorescence (HTRF) Assay kit (62IN2PEG, Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The inhibition of forskolin-stimulated cyclic AMP (cAMP) accumulation by MOR agonists was assessed using the Cisbio Homogeneous Time-Resolved Fluorescence (HTRF) cAMP Gi kit (Cisbio Assays, PerkinElmer). This kit uses a Förster Resonance Energy Transfer (FRET ...
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bioRxiv - Cell Biology 2024Quote: The induction of cAMP was quantified in EnSCs using a modified protocol of the High-Throughput Time Resolved Fluorescence (HTRF) cAMP Gαs dynamic kit (CisBio Bioassays, Codolet, France). A 2-step protocol allowed culture and stimulation of cells in 6-well plates and ensured cAMP levels were within the manufacturer’s detection range ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was stopped by addition of detection buffer (Cisbio, 10 µL) containing (final concentrations) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... After three times washing with wash buffer (Revvity Cisbio), cells were incubated with the anti-βarr2-Tb antibody (Revvity Cisbio ...
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bioRxiv - Cell Biology 2021Quote: ... or Homogeneous Time Resolved Fluorescence (HTRF) assay (glucagon, 62CGLPEH, Cisbio). Area under the curve (AUC ...
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bioRxiv - Bioengineering 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio). After 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched by the addition of 2 µl of 5M followed by the addition of 2 µl Detection Buffer 1 (Cisbio) to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The assay was stopped by cell lysis and the response was measured by adding 20 µl/well detection solution (cAMP conjugate and lysis buffer (Cisbio) + 2.5% anti-cAMP cryptate conjugate (Cisbio ...
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bioRxiv - Immunology 2023Quote: A homogeneous fluorescence resonance energy transfer (FRET) HTRF® (Homogeneous Time-Resolved Fluorescence, Cisbio International) assay was used to measure the inhibition of the IL-33–sST2 interaction ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Inhibition of SMG1 protein kinase activity was assessed using a Homogeneous Time-Resolved Fluorescence (HTRF) assay (Cisbio). 0.8 nM Flag-tagged full-length SMG1 enzyme (produced in HEK293 cells ...
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bioRxiv - Neuroscience 2022Quote: ... The Homogeneous Time Resolved Fluorescence assay (Total alpha Synuclein cellular assay, cat. 6FNSYPEG, Cisbio Bioassays, Codolet, France) was performed according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Labelling was performed using the time-resolved Förster resonance energy transfer (TR-FRET) SNAP-probe Lumi4-Tb (Cisbio) at 40 nM for 60 minutes at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ERK1/2 phosphorylation in HEK293 cells was measured with the HTRF (Homogeneous Time-Resolved Fluorescence) immunoassay from Cisbio, as described previously [23] ...
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bioRxiv - Neuroscience 2020Quote: ... a homogeneous time-resolved fluorescence (HTRF) immunoassay was used for LRRK2 and pS935-LRRK2 according to manufacturer’s guidelines (Cisbio). Recombinant full-length human G2019S-LRRK2 protein (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... cytokines were detected in cell-free supernatants or whole cell lysates by homogeneous time-resolved fluorescence technology (HTRF, Cisbio). A Human ProcartaPlexTM (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 24 hours post-transfection the cells were dual-labelled with the time-resolved (TR)-FRET probe SNAP-Lumi4-Tb (40 nM, Cisbio) and SNAP-Surface 647 (1 μM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cytosol cAMP was measured using a cell-based homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) method (Cisbio Bioassays) (19,20) ...
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bioRxiv - Immunology 2021Quote: A homogeneous time-resolved fluorescence (hTRF) assay [26] comprised of terbium-labeled anti-human IgG (H+L) (Cisbio, custom label) donor and AF488-labeled anti-HIS (Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... supernatants obtained as described for the LDH release assays were subjected to human IL-1β Homogeneous Time Resolved Fluorescence (HTRF) assays (Cisbio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The cell content was collected and used for IP1 assay (the homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the manufacturer’s directions (Cisbio Bioassays, Perkin Elmer). In brief ...
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bioRxiv - Bioengineering 2021Quote: ... EPIgenous Methyltransferase kit (Cisbio Bioassays) was used for the detection of methyltransferase activity as with nsp14.
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bioRxiv - Cell Biology 2021Quote: ... or Insulin High-Range assay kits (Cisbio). For insulin content assays ...
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bioRxiv - Cell Biology 2022Quote: ... or an Insulin high range kit (Cisbio).