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Citations for CisBio :
1 - 50 of 56 citations for Upstream stimulatory factor 2 USF2 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lumi4-Tb-labelled ACE2 cells were incubated with d2-labelled anti-FLAG tag antibody (2 μg/mL, 1h at room temperature; 61FG2DLF, Cisbio Bioassays), followed by addition of non-labelled RBD (5 nM ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Bioengineering 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Neuroscience 2023Quote: ... phospho- and total ERK1/2 were determined using the two-plate adherent cell protocol (Phosph-ERK1/2 kit, Cisbio), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched by the addition of 2 µl of 5M followed by the addition of 2 µl Detection Buffer 1 (Cisbio) to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HTRF detection reagents (cAMP Dynamic 2 kit, Cisbio) were added at the end of the incubation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ERK1/2 phosphorylation was determined by HTRF (Cisbio Phospho-ERK [Thr202/Tyr204] cellular kit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cAMP was quantified by HTRF (cAMP Dynamic 2, Cisbio), and responses were normalised to that of forskolin ...
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bioRxiv - Neuroscience 2023Quote: 11NQ antibody was labeled (Cisbio-Revvity) with Europium Cryptate (donor fluorophore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cAMP was then assayed by HTRF (Cisbio cAMP Dynamic 2) using a Spectramax i3x plate reader (Molecular Devices) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cAMP was then assayed by HTRF (Cisbio cAMP Dynamic 2) and concentration-response curves constructed with 3-parameter curve fitting in Prism 8.0.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP determined by HTRF (cAMP Dynamic 2 kit, Cisbio) using a Spectramax i3x plate reader (Molecular Devices).
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bioRxiv - Cell Biology 2024Quote: ... or anti-AP2-Tb antibody (Revvity Cisbio) at final concentration of 0.5 nM for 2 h ...
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bioRxiv - Bioengineering 2021Quote: ... 1X α-SAH Tb Cryptate antibody solution was prepared by adding one part α-SAH Tb Cryptate antibody (CisBio) to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... 1X α-SAH Tb Cryptate antibody solution was prepared by adding one part α-SAH Tb Cryptate antibody (CisBio) to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Molecular Biology 2023Quote: ... Tb-conjugated anti-6His antibody (53 ng/ml; Cisbio) in assay buffer containing 25 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2023Quote: ... Tb-conjugated anti-6xHis antibody (53 ng/ml; Cisbio) in assay buffer containing 25 mM HEPES (pH 7.5) ...
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bioRxiv - Pathology 2022Quote: Cardiomyocytes cAMP concentration was assessed with cAMP dynamic 2 kit (Cisbio, Saclay, France), according to the manufacturer instructions.
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bioRxiv - Physiology 2022Quote: ... GLP-1R or GIPR using the cAMP Dynamic 2 cAMP kit (Cisbio, France) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... followed by lysis and cAMP HTRF immunoassay (cAMP Dynamic 2, 62AM4PEB, Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... and 5μL of monoclonal anti-6His-europium cryptate antibody (Cisbio) in detection buffer (20mM of HEPES pH 8.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-6XHIS-D2 antibody (Cisbio 61HISDLF, 1:500 dilution). The plate was centrifuged at 500 g for 5 min and incubated at 4 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-6XHIS-D2 antibody (Cisbio 61HISDLF, 1:500 dilution). The plate was centrifuged at 500 g for 5 min and incubated at 4 °C for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP was determined by homogenous time-resolved fluorescence (HTRF, cAMP Dynamic 2 kit, Cisbio).
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) and insulin content was measured by insulin ultra-sensitive assay kit (Cisbio). Within an experiment ...
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bioRxiv - Cell Biology 2022Quote: ... Terbium cryptate conjugated anti HA antibody was from CisBio (Chiba, Japan).
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bioRxiv - Cancer Biology 2023Quote: ... anti-FLAG M2-Tb cryptate antibody (Cisbio 61FG2TLF, 1:1000 dilution) and anti-6XHIS-D2 antibody (Cisbio 61HISDLF ...
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bioRxiv - Biochemistry 2023Quote: ... 1x anti-GST Eu3+-labelled antibody (from 400x stock (Cisbio, 61GSTKLB))) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with the anti-βarr2-Tb antibody (Revvity Cisbio) or anti-AP2-Tb antibody (Revvity Cisbio ...
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bioRxiv - Cell Biology 2020Quote: Intracellular cAMP and IP1 was determined by HTRF (cAMP Dynamic 2 and IP-one, respectively (CisBio)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After stimulation cells were lysed and cAMP was assayed by immunoassay (Cisbio HTRF cAMP Dynamic 2).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ERK1/2 phosphorylation in HEK293 FPR cell systems was measured with the HTRF immunoassay from Cisbio, as described previously [37] ...
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bioRxiv - Cell Biology 2022Quote: ... dispersed islets were lysed and cAMP assayed by HTRF immunoassay (cAMP Dynamic 2, 62AM4PEB, Cisbio, Codolet, France).
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bioRxiv - Bioengineering 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio). After 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: The accumulation of cAMP was determined using a cAMP dynamic 2 kit (Cisbio, Bioassays, Bagnols sur-Cèze, France). Synaptosomes were incubated for 1 h at 37 ºC (0.67 mg/ml ...
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bioRxiv - Systems Biology 2024Quote: ERK1/2 phosphorylation in HEK293 cells was measured with the HTRF (Homogeneous Time-Resolved Fluorescence) immunoassay from Cisbio, as described previously [23] ...
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bioRxiv - Immunology 2021Quote: ... 1:400 (final concentration) of anti-6His-D2 HTRF antibody – FRET acceptor conjugate (Cisbio) and 1:400 (final concentration ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CXCL10 HTRF assay antibodies were prepared according to the manufacturer’s recommendation (Cisbio, cat #: 62HCX10PEH). 4 μL of the mixture of equal part of diluted CXCL10 Eu Cryptate antibody and CXCL10 d2 antibody was added to PBS diluted supernatant in OptiPlate-384 microplates by a Bravo liquid handler ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5,6,7,8-tetrahydro-6-[(2-phenylethyl)propylamino]) derivative labeled with a red fluorescent probe (PPHT-red) was obtained from Cisbio Bioassays (Bagnolssur-Cèze ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of fluorophore-conjugated antibodies was adjusted following the recommendation from the manufacturer (Cisbio). Experiments were performed in 384 well plates with a final volume of 20 μl per well ...
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bioRxiv - Immunology 2021Quote: ... and 1:400 (final concentration) of anti-strep-Tb antibody FRET donor conjugate (Cisbio, France) to a well of a 384-well plate and incubated for 120 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The product was detected by using an anti-histone H3K9 Me2-Eu(K) antibody (Cisbio) and XL665-conjugated Streptavidin (SA-XL665 ...
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and homogenous time-resolved fluorescence (HTRF) reagents for cAMP (cAMP Dynamic 2) and insulin (Insulin High Range Assay) measurement were obtained from Cisbio. SNAP-Surface probes were obtained from New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Biophysics 2024Quote: ... Labeling with the Tb-cryptate for the LRET experiments was carried out by incubating the β-arrestin 1 with 2 equivalents of Lumi-4 Tb maleimide (CisBio) overnight at 4°C ...