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Citations for CisBio :
1 - 50 of 53 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: The pcDNA plasmid encoding SNAP-tagged human mGluR2 was a gift from Cisbio Bioassays (Codolet ...
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bioRxiv - Plant Biology 2019Quote: The enzymatic activity of ScSAPK8 WT and SnRK2-box and ABA-box mutants was measured using a TR-FRET based assay (Cisbio Kinease - catalog #62ST1PEB). Prior to the enzymatic reaction ...
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bioRxiv - Immunology 2020Quote: ... and the Human CCL2 (MCP-1) Kit (62HCCL2PEG; Cisbio) or Human CXCL10 (IP-10 ...
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bioRxiv - Microbiology 2021Quote: ... PAb Anti Human IgG-Eu cryptate (Cisbio 61HFCKLA, 1:200) was finally added and incubate for 60 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The SNAP-tagged FPR1 or FPR2 plasmids were constructed from Tag-lite pT8-SNAP (Cisbio Bioassays; Codolet, France), pEZ-Lv105-FPR1 (#Z7406 ...
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bioRxiv - Cell Biology 2021Quote: ... or Insulin High-Range assay kits (Cisbio). For insulin content assays ...
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bioRxiv - Cell Biology 2022Quote: ... or an Insulin high range kit (Cisbio).
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated Gephyrin variants was measured using FRET (donor: streptavidin-Tb, acceptor: anti-FLAG-d2, Cisbio). Further HTRF measurement against ‘No Target’ allowed for discrimination of Gephyrin-specific hits.
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bioRxiv - Immunology 2020Quote: ... or Human CXCL10 (IP-10) Kit (62HCX10PEG; Cisbio) was added to detect MCP-1 or IP-10 (Fig ...
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bioRxiv - Cell Biology 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated His-Avimyosin VI (aa 992-1031) was measured using FRET (donor: Streptavidin-Tb cryptate (610SATLB, Cisbio), acceptor ...
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bioRxiv - Physiology 2020Quote: CHO-K1 cells stably expressing the human SNAP-GLP1R (Cisbio) (SNAP-GLP1R:CHO-K1 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and analysed for secreted insulin by HTRF (Insulin High Range Assay, Cisbio). Insulin secretion was expressed relative to that from cells stimulated with 11 mM glucose alone in the same experiment.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Supernatant insulin concentration was determined by HTRF (High Range Insulin kit, Cisbio). Results were normalised to those obtained with 11 mM glucose but no additional agonist ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Secreted insulin in the supernatant was analysed by HTRF (Insulin High Range kit, Cisbio) after dilution and normalised to the concentration in glucose-only treated wells.
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bioRxiv - Immunology 2021Quote: ... The HTRF kits for human IL-1β and TNFa were obtained from Cisbio, the ELISA kit for human and mouse IL-1β was obtained from R&D Systems ...
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bioRxiv - Neuroscience 2019Quote: ... All reactions contained 20 μM high purity S-adenosyl methionine (SAM, CisBio, Bedford, MA, USA) in COMT assay buffer (50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were collected every min for insulin measurement using Insulin High Range Kits (Cisbio Assays, Bedford, MA) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: In vitro activity of human RSK CTKD was evaluated in the HTRF-KinEASE™ assay (Cisbio bioassays). For activity evaluation ...
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bioRxiv - Biophysics 2022Quote: The pcDNA plasmid encoding human mGlu2 with N-terminal FLAG- and SNAP-tags was a gift from Cisbio Bioassays (Perking Elmer ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were collected every min for insulin assay using Insulin High Range Kits (Cat# 62IN1PEG, RRID:AB_2890910, Cisbio Assays, Bedford, MA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and homogenous time-resolved fluorescence (HTRF) reagents for cAMP (cAMP Dynamic 2) and insulin (Insulin High Range Assay) measurement were obtained from Cisbio. SNAP-Surface probes were obtained from New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... CHO-K1 cells (a kind gift from Dr Ben Jones, Imperial College London) stably expressing the human SNAP_GLP1R (Cisbio) (CHO-K1-SNAP_GLP1R ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were collected to quantify the amount of IL-1β by HTRF according to the manufacturer’s protocol for the human IL-1β detection kit (Cisbio). In brief ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The PAb Anti Human IgG-d2 (61HFCDAB) and MAb Anti-6HIS-Tb cryptate Gold (61HI2TLB) were purchased from CisBio Bioassays (Codolet ...
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bioRxiv - Physiology 2020Quote: ... stably expressing human SNAP-GLP-1R (“MIN6B1-SNAP-GLP-1R”)(15) were generated by transfection of a SNAP-GLP-1R vector (Cisbio) followed by G418 selection and maintained in DMEM with 15% FBS ...
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bioRxiv - Immunology 2021Quote: A homogeneous time-resolved fluorescence (hTRF) assay [26] comprised of terbium-labeled anti-human IgG (H+L) (Cisbio, custom label) donor and AF488-labeled anti-HIS (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... 16.7 mM glucose and 45mM KCl treatments were collected and insulin amount determined using a human insulin HTRF assay (Cisbio). Cells infected with lentivirus were incubated for 6 days prior to GSIS assay ...
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bioRxiv - Immunology 2023Quote: ... supernatants obtained as described for the LDH release assays were subjected to human IL-1β Homogeneous Time Resolved Fluorescence (HTRF) assays (Cisbio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The induction of cAMP was quantified in EnSCs using a modified protocol of the High-Throughput Time Resolved Fluorescence (HTRF) cAMP Gαs dynamic kit (CisBio Bioassays, Codolet, France). A 2-step protocol allowed culture and stimulation of cells in 6-well plates and ensured cAMP levels were within the manufacturer’s detection range ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...
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bioRxiv - Neuroscience 2020Quote: ... IP-1 ELISA (Cisbio) assay was then used according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μM Ultrapure SAM (CisBio), 0.14 mM GpppA RNA cap analogue (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μM Ultrapure SAM (CisBio), 0.14 mM GpppA RNA cap analogue (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... 1 µM Ultrapure SAM (Cisbio), 187.5 µM RNA cap analogue (GpppA or GpppG ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-6XHIS-D2 antibody (Cisbio 61HISDLF, 1:500 dilution). The plate was centrifuged at 500 g for 5 min and incubated at 4 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-6XHIS-D2 antibody (Cisbio 61HISDLF, 1:500 dilution). The plate was centrifuged at 500 g for 5 min and incubated at 4 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... 10μl of detection mixture containing Streptavidin-d2 (Cisbio 610SADLF, 1:200), PAb Anti Human IgG-Eu cryptate (Cisbio 61HFCKLA ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-FLAG M2-Tb cryptate antibody (Cisbio 61FG2TLF, 1:1000 dilution) and anti-6XHIS-D2 antibody (Cisbio 61HISDLF ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-FLAG M2-Tb cryptate anti-body (Cisbio 61FG2TLF, 1:1000 dilution) and anti-6XHIS-D2 antibody (Cisbio 61HISDLF ...
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bioRxiv - Immunology 2021Quote: ... 1:400 (final concentration) of anti-6His-D2 HTRF antibody – FRET acceptor conjugate (Cisbio) and 1:400 (final concentration ...
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bioRxiv - Immunology 2021Quote: ... and 1:400 (final concentration) of anti-strep-Tb antibody FRET donor conjugate (Cisbio, France) to a well of a 384-well plate and incubated for 120 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ1-40 was determined using an HTRF amyloid beta 1-40 kit (Cisbio, Codolet, France). The protocol has been described previously [42] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The HDAC-1 histone deacetylase assay was tested using the FLUOR DE LYS platform (Cisbio, Bedford, MA). Inhibition of SIRT-1 histone deacetylase was tested with the AlphaLISA detection system ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... was detected by addition of 1 nM Europium-labeled anti-p53 phosphoserine 15 antibody (New England Biolabs/ Cisbio) and 40 nM streptavidin-conjugated APC (Prozyme ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenates were diluted 1:50 and InsP levels were assessed with the IP-One ELISA kit (Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Inositol 1 phosphate (IP1) accumulation in hPC-PL was measured using the HTRF IP-One Gαq Detection Kit (Cisbio). This assay enables the direct pharmacological activity of Gαq - coupled receptors ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched by the addition of 2 µl of 5M followed by the addition of 2 µl Detection Buffer 1 (Cisbio) to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 5 µl of d2-cGMP analogue and 5 µl mAb-cryptate were added to each well and incubated for 1 hour at room temperature as per the manufacturer’s instruction (Cisbio, France). The d2-cGMP fluorophore was excited at a wavelength of 337 nm and emission was detected at 665 nm and 620 nm ...