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Citations for CisBio :
51 - 58 of 58 citations for Ephrin type A receptor 2 EphA2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and homogenous time-resolved fluorescence (HTRF) reagents for cAMP (cAMP Dynamic 2) and insulin (Insulin High Range Assay) measurement were obtained from Cisbio. SNAP-Surface probes were obtained from New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lumi4-Tb-labelled ACE2 cells were incubated with d2-labelled anti-FLAG tag antibody (2 μg/mL, 1h at room temperature; 61FG2DLF, Cisbio Bioassays), followed by addition of non-labelled RBD (5 nM ...
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bioRxiv - Biophysics 2024Quote: ... Labeling with the Tb-cryptate for the LRET experiments was carried out by incubating the β-arrestin 1 with 2 equivalents of Lumi-4 Tb maleimide (CisBio) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions ...