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Citations for CisBio :
101 - 140 of 140 citations for EnzyFluo AMPK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Stimulation solution was replaced by a lysis buffer (IP one HTRF Kit, Cisbio, France) during 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... IP1 concentrations were measured from cell lysates using the HTRF IP-One immunoassay kit (CisBio). Fluorescence was measured and IP1 levels were quantified using the same methodology as the cAMP assay.
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was analyzed in the target cells using “Cleaved PARP (Asp214) Cellular Kit” from CisBio according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... The accumulated cAMP levels were quantified using the CisBIO cAMP dymanic HTRF kit (Cisbio, 62AM4PEC) according to the manufacturer’s instructions and measured on an EnVision plate reader (PerkinElmer) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP was determined by homogenous time-resolved fluorescence (HTRF, cAMP Dynamic 2 kit, Cisbio).
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bioRxiv - Neuroscience 2023Quote: ... Aβ1-40 was determined using an HTRF amyloid beta 1-40 kit (Cisbio, Codolet, France). The protocol has been described previously [42] ...
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bioRxiv - Neuroscience 2020Quote: ... subsequently lysed in cell lysis buffer from the IP-One-Gq kit (Cisbio Bioassays, Codolet, France) and frozen to -20°C ...
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bioRxiv - Physiology 2019Quote: ... Secreted and total insulin were quantified using a homogeneous time-resolved fluorescence (HTRF) insulin kit (Cisbio) in a PHERAstar reader (BMG Labtech ...
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bioRxiv - Physiology 2020Quote: Receptor-mediated intracellular changes in IP1 second messenger were measured using IP-one- Gq kit (Cisbio) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Secreted insulin was detected using a commercially available insulin detection kit (PerkinElmer/Cisbio Bioassays, Bedford, MA) based on homogenous time-resolved fluorescence resonance energy transfer (HTRF ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed and cAMP concentrations were measured using the cAMP Gs dynamic kit (CisBio, Cat# 62AM4PEC) on the FlexStation 3 (Molecular Devices ...
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bioRxiv - Bioengineering 2021Quote: ... Released SAH was detected through the use of the commercially available EPIgeneous™ methyltransferase kit (CisBio Bioassays). Individual kit reagents were reconstituted according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... Released SAH was detected through the use of the commercially available EPIgeneous™ methyltransferase kit (CisBio Bioassays). Individual kit reagents were reconstituted according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2020Quote: ... Secreted and total insulin were quantified using a Homogeneous Time Resolved Fluorescence (HTRF) insulin kit (Cisbio, France) in a PHERAstar reader (BMG Labtech ...
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bioRxiv - Biophysics 2023Quote: ... The amount of cAMP produced in each reaction was determined using the cAMP Gs dynamic kit (Cisbio), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Accumulation of IP1 was measured using the IP-One Gq Homogenous Time-Resolved Fluorescence (HTRF) kit from Cisbio per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: The accumulation of cAMP was determined using a cAMP dynamic 2 kit (Cisbio, Bioassays, Bagnols sur-Cèze, France). Synaptosomes were incubated for 1 h at 37 ºC (0.67 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: βarr1 and βarr2 expression in cells was measured using a total βarr1 and βarr2 cellular kit (Revvity Cisbio), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: βarr2/AP2 interaction in cells was measured using a β-arrestin2/AP2 interaction (62BDBAR2PEB) cellular kit (Revvity Cisbio), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were collected to quantify the amount of IL-1β by HTRF according to the manufacturer’s protocol for the human IL-1β detection kit (Cisbio). In brief ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A sample of supernatant was removed and analysed for insulin content by HTRF (Wide Range Insulin HTRF Kit, Cisbio). Results were expressed relative to insulin released with 11 mM glucose alone.
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bioRxiv - Neuroscience 2023Quote: ... phospho- and total ERK1/2 were determined using the two-plate adherent cell protocol (Phosph-ERK1/2 kit, Cisbio), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenates were diluted 1:50 and InsP levels were assessed with the IP-One ELISA kit (Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Inositol 1 phosphate (IP1) accumulation in hPC-PL was measured using the HTRF IP-One Gαq Detection Kit (Cisbio). This assay enables the direct pharmacological activity of Gαq - coupled receptors ...
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG ...
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bioRxiv - Microbiology 2022Quote: The methyltransferase activity of wild type heterotrimer nsp10/14/16 or the heterotrimer with mutated nsp14 protein (ExoN mutant and catalytic mutant – D90A and E92A) was measured using the EPIgeneous Methyltransferase kit from Cisbio as previously described32 ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed as in the primary screen and IL-1β in the cell- free supernatants was detected using a commercial mouse IL-1β homogenous time-resolved fluorescence (HTRF) kit (Cisbio) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Cell Biology 2023Quote: Insulin concentration (ng/ml or pM) was measured using rat insulin ultra-sensitive ELISA kit (Cat#62IN2PEG, Cisbio, Cambridge, England) or human insulin ELISA kit (Cat#90095 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: FPR-mediated inhibition of cellular de novo cAMP formation was quantified by competitive immunoassays based on the time-resolved measurement of fluorescence resonance energy transfer (HTRF, Homogeneous Time-Resolved Fluorescence) using the cAMP-Gi Kit from Cisbio as previously published [37] ...
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bioRxiv - Cell Biology 2020Quote: ... The secretion medium were then collected to measure the insulin and proinsulin concentrations using respectively an insulin HTRF kit (Cisbio Bioassays) and a rat/mouse proinsulin ELISA kit (Mercodia).
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bioRxiv - Neuroscience 2020Quote: ... After incubation for 1 hour at 37°C the IP1 accumulation was stopped by addition of 40 μL CisBio IP-One Tb HTRF Kit (CisBio, 62IPAPEC) lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: Phosporylated Ubiqutin at Ser65 levels were measured using time-resolved fluorescence resonance energy transfer (FRET) Phospo-Ubiqutin (Ser65) Cellular Kit (Cisbio 64UBIS65PEG) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Hormones from blood samples of Gcgr β-cell-/- mice and littermate controls were measured using standard ELISA kits (for insulin and glucagon, 62IN3PEF and 62SGLPEB, Cisbio, France ...
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bioRxiv - Systems Biology 2024Quote: FPR agonist-induced changes in the cellular de novo cAMP formation were measured with the HTRF-based competitive cAMP-Gi Kit (Cisbio, Revity) as previously published [23] ...
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bioRxiv - Neuroscience 2023Quote: The quantification of synuclein aggregates was performed using HTRF alpha-synuclein aggregation kit according to manufacturer instruction (#6FASYPEG & #6FASYPEH, Cisbio, London UK). Cell samples were first diluted in water ...
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bioRxiv - Cell Biology 2024Quote: The induction of cAMP was quantified in EnSCs using a modified protocol of the High-Throughput Time Resolved Fluorescence (HTRF) cAMP Gαs dynamic kit (CisBio Bioassays, Codolet, France). A 2-step protocol allowed culture and stimulation of cells in 6-well plates and ensured cAMP levels were within the manufacturer’s detection range ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...
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bioRxiv - Physiology 2019Quote: ... Plasma INS was measured in duplicate using a commercially available RIA kit (Insulin-CT; manufactured by MP Biomedicals–Solon, Ohio, USA and distributed by Cisbio Bioassays, Codolet, France); intra- and inter-assay variations averaged 10.3% and 4% ...