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Citations for CisBio :
1 - 50 of 160 citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... ERK phosphorylation was evaluated using a Homogenous Time-Resolved FRET (HTRF)-based Phospho-ERK (Thr202/Tyr204) cellular kit (Cisbio). Cells were lysed for 30 minutes with the lysis buffer provided and incubated for 2 hours with pERK1/2-specific antibodies conjugated with Eu3+-cryptate donor and d2 acceptor at recommended dilutions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HTRF detection reagents (cAMP Dynamic 2 kit, Cisbio) were added at the end of the incubation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cytosol cAMP was measured using a cell-based homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) method (Cisbio Bioassays) (19,20) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Plant Biology 2019Quote: The enzymatic activity of ScSAPK8 WT and SnRK2-box and ABA-box mutants was measured using a TR-FRET based assay (Cisbio Kinease - catalog #62ST1PEB). Prior to the enzymatic reaction ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Developmental Biology 2021Quote: We used a TR-FRET cAMP assay (Cisbio) for estimating cAMP as these assays eliminate the background noise due to FRET-based estimation of signals ...
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bioRxiv - Bioengineering 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2022Quote: TR-FRET assay was performed using recommended conditions from Cisbio with slight modifications ...
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bioRxiv - Biochemistry 2023Quote: ... and the FRET acceptor mAb anti-FLAG M2-d2 (61FG2DLB, Cisbio) and an incubation time of 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... After mixing with the FRET donor Streptavidin-Tb cryptate (610SATLB, Cisbio) and the FRET acceptor mAb anti-FLAG M2-d2 (61FG2DLB ...
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bioRxiv - Neuroscience 2023Quote: ... phospho- and total ERK1/2 were determined using the two-plate adherent cell protocol (Phosph-ERK1/2 kit, Cisbio), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Phosporylated Ubiqutin at Ser65 levels were measured using time-resolved fluorescence resonance energy transfer (FRET) Phospo-Ubiqutin (Ser65) Cellular Kit (Cisbio 64UBIS65PEG) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1:400 (final concentration) of anti-6His-D2 HTRF antibody – FRET acceptor conjugate (Cisbio) and 1:400 (final concentration ...
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bioRxiv - Immunology 2021Quote: ... and 1:400 (final concentration) of anti-strep-Tb antibody FRET donor conjugate (Cisbio, France) to a well of a 384-well plate and incubated for 120 min at 4°C ...
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bioRxiv - Immunology 2023Quote: A homogeneous fluorescence resonance energy transfer (FRET) HTRF® (Homogeneous Time-Resolved Fluorescence, Cisbio International) assay was used to measure the inhibition of the IL-33–sST2 interaction ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed once with cold PBS and harvested in 50 μL FRET lysis buffer (Cisbio) per well and analyzed according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched by the addition of 2 µl of 5M followed by the addition of 2 µl Detection Buffer 1 (Cisbio) to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Labelling was performed using the time-resolved Förster resonance energy transfer (TR-FRET) SNAP-probe Lumi4-Tb (Cisbio) at 40 nM for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 24 hours post-transfection the cells were dual-labelled with the time-resolved (TR)-FRET probe SNAP-Lumi4-Tb (40 nM, Cisbio) and SNAP-Surface 647 (1 μM ...
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated Gephyrin variants was measured using FRET (donor: streptavidin-Tb, acceptor: anti-FLAG-d2, Cisbio). Further HTRF measurement against ‘No Target’ allowed for discrimination of Gephyrin-specific hits.
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bioRxiv - Cell Biology 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated His-Avimyosin VI (aa 992-1031) was measured using FRET (donor: Streptavidin-Tb cryptate (610SATLB, Cisbio), acceptor ...
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bioRxiv - Bioengineering 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio). After 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: Secreted insulin was detected using a commercially available insulin detection kit (PerkinElmer/Cisbio Bioassays, Bedford, MA) based on homogenous time-resolved fluorescence resonance energy transfer (HTRF ...
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bioRxiv - Biochemistry 2024Quote: ... The 5 µl of reaction mixture was transformed to HTRF 96 well low volume plates (Cisbio) and the consumption of ATP was measured with ADP-Glo Kinase Assay kit (Promega Corporation ...
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bioRxiv - Systems Biology 2024Quote: FPR agonist-induced changes in the cellular de novo cAMP formation were measured with the HTRF-based competitive cAMP-Gi Kit (Cisbio, Revity) as previously published [23] ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP determined by HTRF (cAMP Dynamic 2 kit, Cisbio) using a Spectramax i3x plate reader (Molecular Devices).
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were collected to quantify the amount of IL-1β by HTRF according to the manufacturer’s protocol for the human IL-1β detection kit (Cisbio). In brief ...
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bioRxiv - Molecular Biology 2023Quote: IP1 accumulation assay was applied for detection of the downstream Gαq signal of MCHR2 using the IP-One HTRF kit (Cisbio). AD293 cells were cultured in DMEM/ high Glucose supplemented with 10% (v/v ...
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bioRxiv - Physiology 2023Quote: Inositol 1 phosphate (IP1) accumulation in hPC-PL was measured using the HTRF IP-One Gαq Detection Kit (Cisbio). This assay enables the direct pharmacological activity of Gαq - coupled receptors ...
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG; phospho-Yap [Ser127] Cellular Kit, Cisbio 64YapPEG). The signal was detected by Synergy NEO (Biotek).
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bioRxiv - Pathology 2022Quote: Cardiomyocytes cAMP concentration was assessed with cAMP dynamic 2 kit (Cisbio, Saclay, France), according to the manufacturer instructions.
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bioRxiv - Physiology 2022Quote: ... GLP-1R or GIPR using the cAMP Dynamic 2 cAMP kit (Cisbio, France) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... 10μl of detection mixture containing Streptavidin-d2 (Cisbio 610SADLF, 1:200), PAb Anti Human IgG-Eu cryptate (Cisbio 61HFCKLA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP was determined by homogenous time-resolved fluorescence (HTRF, cAMP Dynamic 2 kit, Cisbio).
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) and insulin content was measured by insulin ultra-sensitive assay kit (Cisbio). Within an experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was stopped by addition of detection buffer (Cisbio, 10 µL) containing (final concentrations) ...
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG ...
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bioRxiv - Neuroscience 2022Quote: The accumulation of cAMP was determined using a cAMP dynamic 2 kit (Cisbio, Bioassays, Bagnols sur-Cèze, France). Synaptosomes were incubated for 1 h at 37 ºC (0.67 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... 3 nM XL665-conjugated streptavidin (Cisbio, 610SAXLB), 1x anti-GST Eu3+-labelled antibody (from 400x stock (Cisbio ...
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were exposure to tested compounds by addition of 0.5ml of each with 50 mM Lithium Chloride (LiCl) needed for IP-One accumulation (based on Cisbio IP-One HTRF assay) and dissolved in 0.1 % DMEM (without glucose) ...
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bioRxiv - Immunology 2022Quote: ... Eu-Protein A (5 nM, Cisbio), Streptavidin-Alexa Fluor 647 (6.25 nM ...
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bioRxiv - Immunology 2022Quote: ... Eu-Protein A (5 nM, Cisbio), Streptavidin-Alexa Fluor 647 (6.25 nM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...