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Citations for CisBio :
101 - 148 of 148 citations for Human Oxidation resistance protein 1 OXR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Released SAH was detected through the use of the commercially available EPIgeneous™ methyltransferase kit (CisBio Bioassays). Individual kit reagents were reconstituted according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2020Quote: ... Secreted and total insulin were quantified using a Homogeneous Time Resolved Fluorescence (HTRF) insulin kit (Cisbio, France) in a PHERAstar reader (BMG Labtech ...
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bioRxiv - Biophysics 2023Quote: ... The amount of cAMP produced in each reaction was determined using the cAMP Gs dynamic kit (Cisbio), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Total insulin content was assessed using ultra-sensitive insulin homogeneous time-resolved fluorescence (HTRF) assay kits (Cisbio).
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bioRxiv - Microbiology 2021Quote: ... 10μl of detection mixture containing Streptavidin-d2 (Cisbio 610SADLF, 1:200), PAb Anti Human IgG-Eu cryptate (Cisbio 61HFCKLA ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-FLAG M2-Tb cryptate antibody (Cisbio 61FG2TLF, 1:1000 dilution) and anti-6XHIS-D2 antibody (Cisbio 61HISDLF ...
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bioRxiv - Cell Biology 2021Quote: ... Accumulation of IP1 was measured using the IP-One Gq Homogenous Time-Resolved Fluorescence (HTRF) kit from Cisbio per the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The IP1-d2 conjugate and the anti-IP1 cryptate Tb conjugate (IP-One Tb™ assay kit, CisBio) were diluted together 1:40 in lysis buffer and 3 μl of the antibody mix were added to each well ...
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bioRxiv - Neuroscience 2022Quote: The accumulation of cAMP was determined using a cAMP dynamic 2 kit (Cisbio, Bioassays, Bagnols sur-Cèze, France). Synaptosomes were incubated for 1 h at 37 ºC (0.67 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: βarr1 and βarr2 expression in cells was measured using a total βarr1 and βarr2 cellular kit (Revvity Cisbio), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: βarr2/AP2 interaction in cells was measured using a β-arrestin2/AP2 interaction (62BDBAR2PEB) cellular kit (Revvity Cisbio), according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-FLAG M2-Tb cryptate anti-body (Cisbio 61FG2TLF, 1:1000 dilution) and anti-6XHIS-D2 antibody (Cisbio 61HISDLF ...
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bioRxiv - Biophysics 2022Quote: ... ERK phosphorylation was evaluated using a Homogenous Time-Resolved FRET (HTRF)-based Phospho-ERK (Thr202/Tyr204) cellular kit (Cisbio). Cells were lysed for 30 minutes with the lysis buffer provided and incubated for 2 hours with pERK1/2-specific antibodies conjugated with Eu3+-cryptate donor and d2 acceptor at recommended dilutions ...
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bioRxiv - Neuroscience 2021Quote: IP1 accumulation assay: Inositol monophosphate accumulation was determined using the IP-One HTRF (Homogenous Time Resolved Fluorescence) kit (Cisbio Bioassays ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A sample of supernatant was removed and analysed for insulin content by HTRF (Wide Range Insulin HTRF Kit, Cisbio). Results were expressed relative to insulin released with 11 mM glucose alone.
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bioRxiv - Neuroscience 2023Quote: ... phospho- and total ERK1/2 were determined using the two-plate adherent cell protocol (Phosph-ERK1/2 kit, Cisbio), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: IP1 accumulation assay was applied for detection of the downstream Gαq signal of MCHR2 using the IP-One HTRF kit (Cisbio). AD293 cells were cultured in DMEM/ high Glucose supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were collected every min for insulin assay using Insulin High Range Kits (Cat# 62IN1PEG, RRID:AB_2890910, Cisbio Assays, Bedford, MA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed as in the primary screen and IL-1β in the cell- free supernatants was detected using a commercial mouse IL-1β homogenous time-resolved fluorescence (HTRF) kit (Cisbio) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The accumulative cAMP assay was performed as previously described,45 using the CisBio cAMP-Gi kit (Cisbio Bioasays, Codolet, France). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: FPR-mediated inhibition of cellular de novo cAMP formation was quantified by competitive immunoassays based on the time-resolved measurement of fluorescence resonance energy transfer (HTRF, Homogeneous Time-Resolved Fluorescence) using the cAMP-Gi Kit from Cisbio as previously published [37] ...
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bioRxiv - Immunology 2021Quote: ... 1:400 (final concentration) of anti-6His-D2 HTRF antibody – FRET acceptor conjugate (Cisbio) and 1:400 (final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... The secretion medium were then collected to measure the insulin and proinsulin concentrations using respectively an insulin HTRF kit (Cisbio Bioassays) and a rat/mouse proinsulin ELISA kit (Mercodia).
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bioRxiv - Neuroscience 2020Quote: ... After incubation for 1 hour at 37°C the IP1 accumulation was stopped by addition of 40 μL CisBio IP-One Tb HTRF Kit (CisBio, 62IPAPEC) lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: Phosporylated Ubiqutin at Ser65 levels were measured using time-resolved fluorescence resonance energy transfer (FRET) Phospo-Ubiqutin (Ser65) Cellular Kit (Cisbio 64UBIS65PEG) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: FPR agonist-induced changes in the cellular de novo cAMP formation were measured with the HTRF-based competitive cAMP-Gi Kit (Cisbio, Revity) as previously published [23] ...
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bioRxiv - Biochemistry 2019Quote: ... Activity of hACs was determined in a volume of 10 μl using an Assist Plus pipetting robot (Integra Biosciences, Biebertal, Germany) and a cAMP assay kit from Cisbio (Codolet, France) according to the supplier’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were stored at −20°C until the insulin concentration was determined using an Insulin Ultra-Sensitive Homogeneous Time Resolved Fluorescence (HTRF) Assay kit (62IN2PEG, Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were stored at -20°C until the insulin concentration was determined using an Insulin Ultra-Sensitive HTRF Assay kit (62IN2PEG, Cisbio, Codolet, France) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: The quantification of synuclein aggregates was performed using HTRF alpha-synuclein aggregation kit according to manufacturer instruction (#6FASYPEG & #6FASYPEH, Cisbio, London UK). Cell samples were first diluted in water ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The inhibition of forskolin-stimulated cyclic AMP (cAMP) accumulation by MOR agonists was assessed using the Cisbio Homogeneous Time-Resolved Fluorescence (HTRF) cAMP Gi kit (Cisbio Assays, PerkinElmer). This kit uses a Förster Resonance Energy Transfer (FRET ...
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bioRxiv - Cell Biology 2024Quote: The induction of cAMP was quantified in EnSCs using a modified protocol of the High-Throughput Time Resolved Fluorescence (HTRF) cAMP Gαs dynamic kit (CisBio Bioassays, Codolet, France). A 2-step protocol allowed culture and stimulation of cells in 6-well plates and ensured cAMP levels were within the manufacturer’s detection range ...
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bioRxiv - Immunology 2021Quote: ... and 1:400 (final concentration) of anti-strep-Tb antibody FRET donor conjugate (Cisbio, France) to a well of a 384-well plate and incubated for 120 min at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...
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bioRxiv - Biochemistry 2022Quote: ... The homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the Cisbio IP-One Gq kit (Cisbio Bioassays, Perkin Elmer). Dose-response curves were fitted by Prism 8 (Graphpad) ...
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bioRxiv - Physiology 2019Quote: ... Plasma INS was measured in duplicate using a commercially available RIA kit (Insulin-CT; manufactured by MP Biomedicals–Solon, Ohio, USA and distributed by Cisbio Bioassays, Codolet, France); intra- and inter-assay variations averaged 10.3% and 4% ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The HDAC-1 histone deacetylase assay was tested using the FLUOR DE LYS platform (Cisbio, Bedford, MA). Inhibition of SIRT-1 histone deacetylase was tested with the AlphaLISA detection system ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... was detected by addition of 1 nM Europium-labeled anti-p53 phosphoserine 15 antibody (New England Biolabs/ Cisbio) and 40 nM streptavidin-conjugated APC (Prozyme ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched by the addition of 2 µl of 5M followed by the addition of 2 µl Detection Buffer 1 (Cisbio) to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 5 µl of d2-cGMP analogue and 5 µl mAb-cryptate were added to each well and incubated for 1 hour at room temperature as per the manufacturer’s instruction (Cisbio, France). The d2-cGMP fluorophore was excited at a wavelength of 337 nm and emission was detected at 665 nm and 620 nm ...
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bioRxiv - Cell Biology 2022Quote: ... the range of protein membrane concentration used was from 1 to 25μg in the presence of 10nM of the antagonist NAPS-d2 ligand (L0002 red, Cisbio Bioassays). The FA was measured on a Tecan Infinite M1000 Pro microplate reader (Männedorf ...
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bioRxiv - Genetics 2022Quote: ... A 36 μl aliquot of this lysate was subsequently transferred to a 384 well plate containing 4 μl of the antibody solution (1:50 dilution of anti-ELP1 d2(CisBio) and anti-ELP1 K(CisBio ...
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bioRxiv - Biophysics 2024Quote: ... Labeling with the Tb-cryptate for the LRET experiments was carried out by incubating the β-arrestin 1 with 2 equivalents of Lumi-4 Tb maleimide (CisBio) overnight at 4°C ...