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Citations for CisBio :
101 - 134 of 134 citations for Biotin Z Antibody Internalization Kit rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: βarr2/AP2 interaction in cells was measured using a β-arrestin2/AP2 interaction (62BDBAR2PEB) cellular kit (Revvity Cisbio), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were collected to quantify the amount of IL-1β by HTRF according to the manufacturer’s protocol for the human IL-1β detection kit (Cisbio). In brief ...
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bioRxiv - Biophysics 2022Quote: ... ERK phosphorylation was evaluated using a Homogenous Time-Resolved FRET (HTRF)-based Phospho-ERK (Thr202/Tyr204) cellular kit (Cisbio). Cells were lysed for 30 minutes with the lysis buffer provided and incubated for 2 hours with pERK1/2-specific antibodies conjugated with Eu3+-cryptate donor and d2 acceptor at recommended dilutions ...
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bioRxiv - Neuroscience 2021Quote: IP1 accumulation assay: Inositol monophosphate accumulation was determined using the IP-One HTRF (Homogenous Time Resolved Fluorescence) kit (Cisbio Bioassays ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A sample of supernatant was removed and analysed for insulin content by HTRF (Wide Range Insulin HTRF Kit, Cisbio). Results were expressed relative to insulin released with 11 mM glucose alone.
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bioRxiv - Neuroscience 2023Quote: ... phospho- and total ERK1/2 were determined using the two-plate adherent cell protocol (Phosph-ERK1/2 kit, Cisbio), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenates were diluted 1:50 and InsP levels were assessed with the IP-One ELISA kit (Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: IP1 accumulation assay was applied for detection of the downstream Gαq signal of MCHR2 using the IP-One HTRF kit (Cisbio). AD293 cells were cultured in DMEM/ high Glucose supplemented with 10% (v/v ...
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bioRxiv - Physiology 2023Quote: Inositol 1 phosphate (IP1) accumulation in hPC-PL was measured using the HTRF IP-One Gαq Detection Kit (Cisbio). This assay enables the direct pharmacological activity of Gαq - coupled receptors ...
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were collected every min for insulin assay using Insulin High Range Kits (Cat# 62IN1PEG, RRID:AB_2890910, Cisbio Assays, Bedford, MA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG ...
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bioRxiv - Microbiology 2022Quote: The methyltransferase activity of wild type heterotrimer nsp10/14/16 or the heterotrimer with mutated nsp14 protein (ExoN mutant and catalytic mutant – D90A and E92A) was measured using the EPIgeneous Methyltransferase kit from Cisbio as previously described32 ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed as in the primary screen and IL-1β in the cell- free supernatants was detected using a commercial mouse IL-1β homogenous time-resolved fluorescence (HTRF) kit (Cisbio) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The accumulative cAMP assay was performed as previously described,45 using the CisBio cAMP-Gi kit (Cisbio Bioasays, Codolet, France). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: FPR-mediated inhibition of cellular de novo cAMP formation was quantified by competitive immunoassays based on the time-resolved measurement of fluorescence resonance energy transfer (HTRF, Homogeneous Time-Resolved Fluorescence) using the cAMP-Gi Kit from Cisbio as previously published [37] ...
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bioRxiv - Cell Biology 2020Quote: ... The secretion medium were then collected to measure the insulin and proinsulin concentrations using respectively an insulin HTRF kit (Cisbio Bioassays) and a rat/mouse proinsulin ELISA kit (Mercodia).
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bioRxiv - Neuroscience 2020Quote: ... After incubation for 1 hour at 37°C the IP1 accumulation was stopped by addition of 40 μL CisBio IP-One Tb HTRF Kit (CisBio, 62IPAPEC) lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: Phosporylated Ubiqutin at Ser65 levels were measured using time-resolved fluorescence resonance energy transfer (FRET) Phospo-Ubiqutin (Ser65) Cellular Kit (Cisbio 64UBIS65PEG) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The cortisol ELISA assay was performed as per manufacturer’s instructions using the Cisbio HTRF cortisol ELISA kit (62CRTPEG, Cisbio, Perkin Elmer) and measurements were performed on Tecan Infinite M1000 Pro multi-plate reader.
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bioRxiv - Physiology 2022Quote: Hormones from blood samples of Gcgr β-cell-/- mice and littermate controls were measured using standard ELISA kits (for insulin and glucagon, 62IN3PEF and 62SGLPEB, Cisbio, France ...
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bioRxiv - Systems Biology 2024Quote: FPR agonist-induced changes in the cellular de novo cAMP formation were measured with the HTRF-based competitive cAMP-Gi Kit (Cisbio, Revity) as previously published [23] ...
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bioRxiv - Biochemistry 2019Quote: ... Activity of hACs was determined in a volume of 10 μl using an Assist Plus pipetting robot (Integra Biosciences, Biebertal, Germany) and a cAMP assay kit from Cisbio (Codolet, France) according to the supplier’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were stored at −20°C until the insulin concentration was determined using an Insulin Ultra-Sensitive Homogeneous Time Resolved Fluorescence (HTRF) Assay kit (62IN2PEG, Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were stored at -20°C until the insulin concentration was determined using an Insulin Ultra-Sensitive HTRF Assay kit (62IN2PEG, Cisbio, Codolet, France) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: The quantification of synuclein aggregates was performed using HTRF alpha-synuclein aggregation kit according to manufacturer instruction (#6FASYPEG & #6FASYPEH, Cisbio, London UK). Cell samples were first diluted in water ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The inhibition of forskolin-stimulated cyclic AMP (cAMP) accumulation by MOR agonists was assessed using the Cisbio Homogeneous Time-Resolved Fluorescence (HTRF) cAMP Gi kit (Cisbio Assays, PerkinElmer). This kit uses a Förster Resonance Energy Transfer (FRET ...
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bioRxiv - Cell Biology 2024Quote: The induction of cAMP was quantified in EnSCs using a modified protocol of the High-Throughput Time Resolved Fluorescence (HTRF) cAMP Gαs dynamic kit (CisBio Bioassays, Codolet, France). A 2-step protocol allowed culture and stimulation of cells in 6-well plates and ensured cAMP levels were within the manufacturer’s detection range ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...
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bioRxiv - Biochemistry 2022Quote: ... The homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the Cisbio IP-One Gq kit (Cisbio Bioassays, Perkin Elmer). Dose-response curves were fitted by Prism 8 (Graphpad) ...
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bioRxiv - Physiology 2019Quote: ... Plasma INS was measured in duplicate using a commercially available RIA kit (Insulin-CT; manufactured by MP Biomedicals–Solon, Ohio, USA and distributed by Cisbio Bioassays, Codolet, France); intra- and inter-assay variations averaged 10.3% and 4% ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions ...