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201 - 250 of 1505 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020

• Capsaicin-induced endocytosis of endogenous presynaptic Ca_V2.2 in DRG-spinal cord co-cultures inhibits presynaptic function

  Krishma H Ramgoolam, Annette C Dolphin, bioRxiv - Neuroscience 2022

  Quote: ... neurons were incubated with rabbit-anti-RIM 1/2 (1:200; Synaptic Systems) antibody overnight at 4°C ...
• Gluk4-containing kainate receptors regulate synaptic communication in the motor cortex and reduce axon degeneration in adult mice

  Raphael Ricci, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse anti-vesicular glutamate transporter 1 (VGlut1, 1:200, Synaptic Systems, #135 511), and chicken anti-vesicular GABA transporter (VGAT ...
• Targeted sensors for glutamatergic neurotransmission

  Yuchen Hao, et al., bioRxiv - Neuroscience 2021

  Quote: ... blocked in PBS-T containing 5% normal donkey-serum and incubated overnight at 4°C with chicken monoclonal antibody against Flag M2 (1:2000; Chemicon, AB5543) and guinea pig polyclonal antibody VGLUT 1 (1:4000; Synaptic System, 135304). Primary antibodies were labeled with Alexa Fluor 405 Affinipure donkey anti-chicken IgG and Alexa Fluor 647 Affinipure donkey anti-guinea pig IgG (1:500 each ...
• Amyloidosis and ageing have differential effects on microglial Ca²⁺ activity in the mouse brain

  Pablo Izquierdo, et al., bioRxiv - Neuroscience 2023

  Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957], and rabbit anti-Iba1 [1:500, Synaptic Systems 234003]) in blocking buffer for 12 h at 4°C with agitation ...
• A nociceptive amygdala-striatal pathway for chronic pain aversion

  Jessica A. Wojick, et al., bioRxiv - Neuroscience 2024

  Quote: ... chicken anti-GFP [1:1000, Abcam, ab13970], guinea pig anti-FOS [1:1000, Synaptic Systems, 226308], rabbit anti-FOS [1:1000, Synaptic System, 226008],rabbit anti-DsRed [1:1000 ...
• Mimicking opioid analgesia in cortical pain circuits

  Justin G. James, et al., bioRxiv - Neuroscience 2024

  Quote: ... chicken anti-GFP [1:1000, Abcam, ab13970], guinea pig anti-FOS [1:1000, Synaptic Systems, 226308], rabbit anti-FOS [1:1000, Synaptic System, 226008],rabbit anti-DsRed [1:1000 ...
• A brain-body feedback loop driving HPA-axis dysfunction in breast cancer

  Adrian Gomez, et al., bioRxiv - Neuroscience 2024

  Quote: ... sections were placed in primary antibody (cFos, 1:2000, Cell Signaling, #2250; Vglut, 1:500, Invitrogen, #PA5-119621; Vgat, 1:500, Synaptic Systems, #131-004;-Homer ...
• Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1

  Maria Tsemperouli, et al., bioRxiv - Biophysics 2024

  Quote: ... Molecular weight regions above 50 kDa were cut from the membrane and incubated overnight at 4°C with primary antibodies against Syt1 (cat# 105 011, Synaptic Systems, Goettingen, Germany) and the region below 50 kDa was incubated with anti-GAPDH (cat#2198 ...
• The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects

  Erwan Lambert, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-NA/K ATPase alpha subunit (a5, DSHB, 1/100), anti-rhodopsin (4C5, DSHB, 1/200) and anti-GFP (132004, Synaptic System, 1/100). After washing they were incubated overnight at 4°C with Alexa 555 Phalloidin anti-F-actin (A34055 ...
• The synaptic vesicle protein Mover/TPRG1L is associated with lipid droplets in astrocytes

  Jeremy Krohn, et al., bioRxiv - Neuroscience 2022

  Quote: ... ionized calcium adaptor molecule 1 (IBA1; 1:2000; Synaptic Systems Cat# 234 006, RRID:AB_2619949). Excess of primary antibodies were washed out with PBS ...
• Oligodendrocyte dependent synchronized activity orchestrates circuit maturation and brain functionalization

  Ryo Masumura, et al., bioRxiv - Neuroscience 2024

  Quote: ... an antibody against RFP (390004, 1:1000, Synaptic Systems or PM005, 1:500, MBL), an antibody against MBP (NBP2-50035 ...
• Viral vectors with cluster of differentiation gene promoters to target specific cell types in the brain

  Naofumi Uesaka, et al., bioRxiv - Neuroscience 2024

  Quote: ... an antibody against RFP (390004, 1:1000, Synaptic Systems or PM005, 1:500, MBL), an antibody against neurogranin (AB5620 ...
• Assessment of ethanol-induced toxicity on iPSC-derived human dopaminergic neurons using a novel high-throughput mitochondrial neuronal health (MNH) assay

  Annika Zink, et al., bioRxiv - Cell Biology 2020

  Quote: ... MAP2 (Synaptic System, 1:100), NANOG (R&D Systems ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... vGAT (1:1000, Synaptic Systems), SNAP25 (1:1000 ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... SNAP25 (1:1000, Synaptic Systems). Secondary antibodies Goat anti rabbit-HRP (1:15000 ...
• Tau interactome mapping reveals dynamic processes in synapses and mitochondria associated with neurodegenerative disease

  Tara E. Tracy, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-Synaptotagmin 1 (Synaptic Systems) and rabbit anti-Synapsin (Cell Signaling Technology) ...
• ARPC5 Isoforms and Their Regulation by Calcium-Calmodulin-N-WASP Drive Distinct Arp2/3-dependent Actin Remodeling Events in CD4 T Cells

  Lopamudra Sadhu, et al., bioRxiv - Immunology 2022

  Quote: ... 1:500 (#305011, Synaptic systems), rabbit anti-ARPC5L ...
• Directed differentiation of functional corticospinal-like neurons from endogenous SOX6+/NG2+ cortical progenitors

  Abdulkadir Ozkan, et al., bioRxiv - Neuroscience 2024

  Quote: ... 1:500 (Synaptic Systems, 135302); rabbit anti-2A-peptide ...
• Directed differentiation of functional corticospinal-like neurons from endogenous SOX6+/NG2+ cortical progenitors

  Abdulkadir Ozkan, et al., bioRxiv - Neuroscience 2024

  Quote: ... 1:500 (Synaptic Systems, 106002); mouse anti-SYNAPTOPHYSIN ...
• Complementation of a human disease phenotype in vitro by intercellular mRNA transfer

  Gal Haimovich, et al., bioRxiv - Cell Biology 2024

  Quote: ... 1mg of protein per sample was separated by SDS-PAGE (4-10%) electrophoresis and CRE protein was detected using rabbit anti-Cre-Recombinase antibodies (Synaptic Systems; Cat# 257 003). For loading control ...
• Fast resupply of synaptic vesicles requires Synaptotagmin-3

  Dennis J. Weingarten, et al., bioRxiv - Neuroscience 2021

  Quote: ... then incubated overnight at 4 °C in vehicle with primary antibodies (rabbit anti-Syt3, 1:500, Synaptic Systems 105133; guinea pig anti-vGlut1, 1:500, Synaptic Systems 135304; guinea pig anti-vGlut2, 1:500, Synaptic Systems 135404). After 4 washes for 5 minutes in vehicle ...
• Molecular characteristics and laminar distribution of prefrontal neurons projecting to the mesolimbic system

  Ákos Babiczky, Ferenc Mátyás, bioRxiv - Neuroscience 2022

  Quote: ... Calb1 (rabbit, SWANT: CB38, RRID: AB_10000340; 1:2000; mouse, SWANT: 300, RRID: AB_10000347; 1:2000; chicken, Synaptic Systems: 214 006, RRID: AB_2619903; 1:2000), TH (mouse ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-Iba1 (1:1000; Wako #019-19741; RRID #AB_839504) and guinea pig anti-synaptophysin-1 (1:750; Synaptic Systems #101 004; RRID #AB_1210382). Following the permeabilization step ...
• TRAF6 controls spinogenesis instructing synapse density and neuronal activity through binding neuroplastin

  Sampath Kumar Vemula, et al., bioRxiv - Cell Biology 2020

  Quote: ... Other primary antibodies used were: anti-Synapsin 1 rabbit (Synaptic Systems, #106 103; 1:500), anti-Shank2 guinea pig antibody (Synaptic Systems ...
• SynaptoPAC, an Optogenetic Tool for Induction of Presynaptic Plasticity

  Silvia Oldani, et al., bioRxiv - Neuroscience 2020

  Quote: ... and guinea pig anti-vesicular glutamate transporter 1 (VGLUT1; 1:4000; Synaptic Systems, Göttingen, Germany) primary antibodies at room temperature for 1.5 h ...
• Purkinje cell neurotransmission patterns cerebellar basket cells into zonal modules that are defined by distinct pinceau sizes

  Joy Zhou, et al., bioRxiv - Neuroscience 2020

  Quote: ... Rabbit polyclonal anti-hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1; 1:350; Synaptic Systems), was used to label basket cell axons and pinceau terminals ...
• Retrosplenial inputs drive diverse visual representations in the medial entorhinal cortex

  Olivier Dubanet, Michael J. Higley, bioRxiv - Neuroscience 2023

  Quote: ... or rabbit anti-reelin (1:500 invitrogene) or rabbit anti-calbindin (1:500 Synaptic System) were diluted in blocking solution (0.02 % BSA ...
• Microglial 25-hydroxycholesterol mediates neuroinflammation and neurodegeneration in a tauopathy mouse model

  Danira Toral-Rios, et al., bioRxiv - Neuroscience 2023

  Quote: Immunofluorescence for synaptic markers Synapsin 1/2 (guinea pig polyclonal, 1:500; Synaptic Systems, 106004) and PSD95 (rabbit polyclonal ...
• Chronobiological rhythms control of site- and cell-specific miRNA and mRNA genes and networks across the central nervous system

  Amanda M. Zacharias, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-Iba1 (1:500, cat.no. 019-19741, Wako; 1:500 cat.no. 234 009, Synaptic Systems), anti-GFAP (1:1000 ...
• Cytoplasmic accumulation of FUS triggers early behavioral alterations linked to cortical neuronal hyperactivity and defects in inhibitory synapses

  Jelena Scekic-Zahirovic, et al., bioRxiv - Neuroscience 2020

  Quote: ... Brain sections were incubated with the indicated primary antibodies (Rabbit GABAAalpha3 antibody Synaptic Systems, 1:500; Mouse Gephyrin antibody Synaptic Systems, 1:500, Guinea pig VGAT antibody, Synaptic Systems, 1/500) for 48 hours at 4°C followed by secondary antibodies (1:1000 ...
• Control of neurotransmitter release and presynaptic plasticity by re-orientation of membrane-bound Munc13-1

  Marcial Camacho, et al., bioRxiv - Neuroscience 2021

  Quote: ... blocked and incubated overnight with rabbit polyclonal antibody against Munc13-1 (1:500; Synaptic System 126103), chicken polyclonal antibody against MAP2 (1:2,000 ...
• Midbrain dopaminergic inputs gate amygdala intercalated cell clusters by distinct and cooperative mechanisms

  Ayla Aksoy-Aksel, et al., bioRxiv - Neuroscience 2020

  Quote: ... sheep anti-FoxP2 (RnDSystems, 1:200) and polyclonal guinea pig anti-vGAT (Synaptic Systems, 1:500) or a combination of guinea pig-anti-vGluT1 (Millipore ...
• Dorsal horn CGRP-expressing interneurons contribute to nerve injury-induced mechanical hypersensitivity

  LS Löken, et al., bioRxiv - Neuroscience 2020

  Quote: ... VGLUT1-immunoeractivity was first detected with 1:50,000 or 1:100,000 rabbit anti-VGLUT1 (Synaptic Systems), biotinylated donkey anti-rabbit IgG ...
• The activation of mGluR4 rescues parallel fiber LTP, motor learning and social behavior in a mouse model of Fragile X Syndrome

  Ricardo Martín, et al., bioRxiv - Neuroscience 2022

  Quote: ... and a mouse monoclonal antibody against synaptophysin 1 (1:500, Cat# 101 011: Synaptic Systems, RRID:AB_887822), as described previously (Ferrero et al. ...
• Serotonergic neurons in the dorsal raphe regulate visual attention

  Jonas Lehnert, et al., bioRxiv - Neuroscience 2024

  Quote: ... Primary antibodies were used as follows: rabbit anti-TPH2 (1:1000, Novus; 1:500, Synaptic Systems); chicken anti-GFP (1:1000 ...
• Presynaptic filopodia form kinapses and modulate membrane mechanics for synchronous neurotransmission and seizure generation

  Akanksha Jain, et al., bioRxiv - Neuroscience 2024

  Quote: ... The following rabbit antibodies were used: synaptophysin 1 (WB, 1:1000; Synaptic Systems, 101 002, RRID:AB_1961591); SV2A (ICC ...
• Deciphering the nanoscale architecture of presynaptic actin using a micropatterned presynapse-on-glass model

  Sofia Tumminia, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse anti-synaptotag-min1 (1:600, clone 604.2/aa 1-12, 105 311, RRID:AB_993036; Synaptic Systems), rabbit anti-adducin alpha (1:100 ...
• GBA1 mutations alter neuronal firing and structure, regulating VGLUT2 and CRYAB in dopamine neurons

  Eva Rodríguez-Traver, et al., bioRxiv - Neuroscience 2024

  Quote: ... MAP2 (1:250: Sigma-Aldrich Cat# M1406, RRID:AB_477171 and 1:1000: Synaptic Systems Cat# 188004, RRID:AB_2138181); α-synuclein LB509 (1:800 ...
• Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels

  Marc Duque, et al., bioRxiv - Neuroscience 2021

  Quote: ... NeuN (1:1500; Synaptic Systems 226004), GFP (1:1000 ...
• Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells

  Inês Caldeira Brás, et al., bioRxiv - Neuroscience 2021

  Quote: ... Synaptophysin (1:500, 101002, Synaptic Systems), Tau (1:1000 ...
• Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells

  Inês Caldeira Brás, et al., bioRxiv - Neuroscience 2021

  Quote: ... α-tubulin (1:1000, 302217, Synaptic Systems), β-Actin (1:10000 ...
• Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells

  Inês Caldeira Brás, et al., bioRxiv - Neuroscience 2021

  Quote: ... α-tubulin (1:1000, 302217, Synaptic Systems), Glial Fibrillary Acidic Protein (GFAP ...
• Common molecular mechanisms underlie the transfer of alpha-synuclein, Tau and huntingtin and modulate spontaneous activity in neuronal cells

  Inês Caldeira Brás, et al., bioRxiv - Neuroscience 2021

  Quote: ... Synaptophysin (1:1000, 101002, Synaptic Systems), Tau (1:5000 ...
• Combinatorial small molecule-mediated activation of CBP/p300 with environmental enrichment in chronic severe experimental spinal cord injury to enable axon regeneration and sprouting for functional recovery

  Franziska Mueller, et al., bioRxiv - Neuroscience 2021

  Quote: ... vGlut1 (1:1000, Synaptic system 135302), vGAT1 (1:500 ...
• Combinatorial small molecule-mediated activation of CBP/p300 with environmental enrichment in chronic severe experimental spinal cord injury to enable axon regeneration and sprouting for functional recovery

  Franziska Mueller, et al., bioRxiv - Neuroscience 2021

  Quote: ... vGAT1 (1:500, Synaptic systems 131011), antibodies at 4°C O/N ...
• Presynaptic PTPσ organizes neurotransmitter release machinery at excitatory synapses

  Kyung Ah Han, et al., bioRxiv - Neuroscience 2020

  Quote: ... anti-GABAARγ2 (Synaptic Systems; 1:500), anti-GluA1 (1193 ...
• Presynaptic PTPσ organizes neurotransmitter release machinery at excitatory synapses

  Kyung Ah Han, et al., bioRxiv - Neuroscience 2020

  Quote: ... anti-Gephyrin (Synaptic Systems; 1:100), and anti-pan-Shank (1172 ...
• An Optimized CRISPR/Cas9 Approach for Precise Genome Editing in Neurons

  Huaqiang Fang, et al., bioRxiv - Neuroscience 2020

  Quote: ... anti-Gephyrin (Synaptic Systems, 1:2500), anti-myc (homemade ...
• Long-term synaptic depression triggers local biogenesis of autophagic vesicles in dendrites and requires autophagic degradation

  Emmanouela Kallergi, et al., bioRxiv - Neuroscience 2020

  Quote: ... MAP2 (1:1000, Synaptic Systems, #188004), FIP200 (1:1000 ...
• THE DYNAMIC INTERPLAY BETWEEN HOMEODOMAIN TRANSCRIPTION FACTORS AND CHROMATIN ENVIRONMENT REGULATES PRONEURAL FACTOR OUTCOMES

  Cheen Euong Ang, et al., bioRxiv - Genomics 2020

  Quote: ... rabbit VGLUT1 (1:1000, Synaptic system) and mouse Flag (1:1000 ...