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Citations for Synaptic Systems :
1551 - 1600 of 1638 citations for Anti Beta galactosidase Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... saturated with PBS 2% BSA for 1 hour at RT and stained with primary antibodies specific for whole VAMP-2 (Synaptic System 104 211). After extensive washing with PBS 0.5% BSA ...
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bioRxiv - Pathology 2021Quote: ... Neutralization was determined by monitoring the cleavage of VAMP-2 with a primary antibody specific for the intact form of the protein (Synaptic System 104 211) and an antibody specific for SNAP-25 (clone SMI81 Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were incubated with 50 μg/mL unlabeled Fab fragments directed against TNR (custom-made from the same TNR antibody used in the rest of the work; Synaptic Systems, Göttingen, Germany), applied together with 1 mg/mL unlabeled anti-mouse nanobody ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were incubated for 5 min with 1:100 polyclonal rabbit antibodies directed against the lumenal domain of Syt1 conjugated to the fluorescent dye Oyster488 (#105 103C2; Synaptic Systems, Göttingen, Germany) in either plain Tyrode (stimulated condition ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 1:500 polyclonal rabbit antibodies directed against the lumenal domain of Syt1 conjugated to the fluorescent dye Oyster488 (#105 103C2; Synaptic Systems, Göttingen, Germany) during the TNR labeling step.
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bioRxiv - Neuroscience 2020Quote: ... surface TNR epitopes were blocked with 10 μg/mL unlabeled Fab fragments directed against TNR (custom-made from the same TNR antibody used in the rest of the work; Synaptic Systems, Göttingen, Germany), applied together with 1 mg/mL unlabeled anti-mouse nanobody (custom-made ...
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bioRxiv - Neuroscience 2021Quote: ... and Syt1Ab uptake in the concentration indicated as follows: rabbit antibodies against Syt1 (labelled with Oyster 550; live staining: 1:70, # 105103C3, Synaptic System, Göttingen, Germany), pThr138SNAP25 (WB:1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Nitrocellulose membranes were blocked with 5% milk in TBST buffer and used to detect m6A levels by probing with m6A specific antibodies (Synaptic Systems; 202 003). Images were taken by Amersham Biosciences Imager 600 (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... spontaneously active synaptic boutons were labeled by incubating cells with CypHer5E-conjugated antibodies against the luminal domain of vGAT (1:500 from a 1 mg/ml stock; Synaptic Systems; Cat#131,103CpH) for 2 h in their respective conditioned culture media at 37 °C and 5 % CO2 prior to imaging.
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bioRxiv - Neuroscience 2022Quote: ... the cryo-sections were incubated for 48 h at 4 °C (while shaking) with a solution containing mouse primary antibody against GluA1 (1:1000; Synaptic Systems, Cat# 182011) in 0.01 M PBS with 10 % NGS and 0.1 % Triton X-100.
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bioRxiv - Neuroscience 2024Quote: ... blocking solution for 1 hr at room temperature and incubated overnight at 4 °C with rabbit c-fos antibody (1:5000 dilution, Synaptic Systems, Goettingen, Germany). Following incubation with the primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies included PCP4 (Invitrogen, PA5-52209, 1:500, raised in rabbit, and Synaptic Systems, 480 004, 1:500, raised in Guinea pig), GFP (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... Molecular weight regions above 50 kDa were cut from the membrane and incubated overnight at 4°C with primary antibodies against Syt1 (cat# 105 011, Synaptic Systems, Goettingen, Germany) and the region below 50 kDa was incubated with anti-GAPDH (cat#2198 ...
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bioRxiv - Physiology 2022Quote: ... MA) and a guinea pig anti-vesicular acetylcholine transporter (VAChT 1:300; cat # 139105 from Synaptic Systems). Sympathetic nerve fibers were visualized by two anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2020Quote: ... Glutamatergic neurons were identified by loading Oyster-550-labeled rabbit anti-vGAT (Synaptic Systems Cat# 131-103C3), as previously described (Pan et al. ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 40 h at 4 °C with rabbit anti-c-FOS (Synaptic Systems, 226003) or goat anti-c-FOS (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... and total GluA2 was detected using guinea pig anti-GluA2 (Synaptic Systems, Cat# 182 105; 1:500), followed by incubation in corresponding secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: The following primary antisera were used at the following dilutions: Rabbit anti-GAT1 (Synaptic Systems, 274 102) (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... For protein expression analysis neurons were incubated with guinea-pig polyclonal anti-VGlut1(1:4000; Synaptic Systems), mouse monoclonal anti-STX1A (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-cFOS rabbit monoclonal and guinea pig monoclonal (1:1000; Synaptic Systems, Cat# 226008 and 226308, respectively), anti-Cav3.1 (C-terminal ...
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bioRxiv - Neuroscience 2021Quote: ... cultured hippocampal neurons were incubated live for 10 min at 37 °C with a mouse monoclonal antibody raised against the N-terminal domain of GluA2 that also recognizes GluA1 and GluA3 (Synaptic Systems, 182411 Clone 248B7) and diluted to 1:100 in the culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... 20 μg of protein lysate were loaded and analyzed by SDS-PAGE followed by immunoblotting: CaV2.1 α1 CT antibody (Synaptic Systems, #152 203, rabbit polyclonal) diluted 1:1000 (final concentration 2 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: Vesicular cycling assays were performed using an antibody directed to an extracellular epitope of synaptotagmin 1 (syt, clone 604.2; Synaptic Systems, Kraszewski et al., 1995) that was fed to neurons before fixation ...
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bioRxiv - Cell Biology 2024Quote: ... Synapsin condensates were co-labeled using a rabbit polyclonal antibody made against the IDR of synapsin 1a/b (Synaptic Systems 106 008; 1:100), which was characterized in Supplemental figure 8 ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were probed overnight at 4°C with 1:1,000 rabbit anti-GABA transporter 1 (Synaptic Systems, 274102) or 1:1,000 rabbit anti-GABA transporter 3 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... For DAGLa co-stainings with neuronal or astrocyte markers guniea pig anti-NeuN (1:1000 266004, Synaptic Systems) and chicken anti-GFAP (1:1000 ab4674 ...
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bioRxiv - Biophysics 2020Quote: ... cells were labelled with anti-synapsin Oyster 550 conjugated for staining synapsin (1:600, 106 011C3, Synaptic Systems), anti-βIII-tubulin Alexa Fluor 488 conjugated for staining microtubules (1:300 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were then incubated for 2 nights at 4°C with chicken anti-Iba1 (Synaptic Systems, 234 006) and rabbit anti-RFP (Rockland ...
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bioRxiv - Neuroscience 2023Quote: ... Glutamatergic and GABAergic synaptic terminals were detected using guinea pig anti-VGLUT1 (1:500, Cat# 135304, Synaptic Systems) and guinea pig anti-VGAT (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... containing KP-Cre/ZsGreen neurons were processed to detect the cholinergic marker vesicular acetylcholine transporter (VAChT) through the following steps: Guinea pig polyclonal VAChT antibody (#139 103; Synaptic Systems; 1:1000; 12h; RT), biotin-conjugated anti-guinea pig IgG (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was blocked with 10% NGS and 0.3% Triton X-100 at RT for 1h then incubated in primary antibodies for AnkG (1:500, Cat#386-006, Synaptic Systems, Gao and Heldt, 2016) and GABAA α2 (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... 750 mM NaCl and 0.5% (vol/vol) Igepal CA-630) and RNase-free water and incubated with 10 μg m6A antibody (Synaptic Systems, cat. no. 202 003, Goettingen, Germany). The mixture was rotated at 4 °C for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... and then cells were fixed and permeabilized and incubated a second time with primary antibodies to label synaptophysin (1:1000) (α-SYP; Synaptic Systems Cat# 101 004; RRID:AB_1210382) and pHluorin (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4 °C with polyclonal rabbit anti-cFos (#226 003, Synaptic Systems, RRID: AB_2231974) and monoclonal rat anti-mCherry (#M11217 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were permeabilized and blocked for 12 h and incubated with rabbit anti-IBA1 (1:500, Synaptic Systems, 234006), rat anti-mouse CD68 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: 100 µm thick mouse brain coronal sections were immunostained with guinea pig anti-Iba1 (1:500; #234004, Synaptic Systems), Alexa 647 donkey anti-guinea pig (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were incubated for 7 min at 37 °C with 10 μg.mL-1 mouse anti-GluA (Synaptic Systems, #182411) and anti-γ2 Ex2 or anti-γ8 Ex1 antibodies prior to 4% PFA/sucrose fixation ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at room temperature in cFos anti-guinea pig (1:1000, Synaptic Systems, product code: 226004). The following day ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated overnight at 4°C in rabbit anti-VAChT (3:500, Synaptic Systems, cat# 139103, RRID: AB_887864). This antibody was previously validated by Western blot and has been successfully used to identify cholinergic terminals in the cochlear nucleus and hippocampus (Gillet et al. ...
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bioRxiv - Neuroscience 2022Quote: ... To detect c-fos we used primary polyclonal guinea pig anti-cfos from Synaptic systems (Cat no. 226 004) and secondary anti-guinea pig goat 488 purchased from Jackson Immuno Research (Cat no ...
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bioRxiv - Neuroscience 2022Quote: ... sections were placed in the primary solution of rabbit anti-Fos (226 003, Synaptic Systems,Goettingen, Germany, 1:1000) with 2% donkey serum and 0.5% Triton X-100 in PBC for 48 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Chicken anti-map2 (against residues 2-314 of human map2, 1:1000 for IF) was from Synaptic Systems (#188006). Chicken anti pan-neurofascin (against residues 25-1031 of rat neurofascin ...
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bioRxiv - Neuroscience 2024Quote: ... nonspecific binding sites were blocked and slices were incubated overnight with a rabbit anti-VgluT1 (1:1000, Synaptic System), a mouse anti-VGAT (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: The following primaries and secondaries were used for GFAP and AQP4 immunolabeling: chicken anti-GFAP (1:1000, #173006, Synaptic Systems) and guinea pig anti-AQP4 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were used at 1:300 dilution in PBS with 0.1% Triton and 2% BSA (PBT) overnight at 4°C (Chicken anti-Bassoon Synaptic systems cat#141016 ...
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bioRxiv - Neuroscience 2020Quote: ... guinea pig anti-neuronal nuclei protein (NeuN, also known as hexaribonucleotide-binding protein 3; 1:500, 266004, Synaptic Systems, RRID:AB_2619988); goat anti-nitric oxide synthase (NOS ...
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bioRxiv - Immunology 2023Quote: ... 100 µm thick sections were cut with vibratome and were immunostained with guineapig anti-Iba1 (1:500 #234308, Synaptic Systems), Alexa 647 donkey anti-guineapig (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... were bound overnight at 4°C with tumbling end-over-end to rabbit polycolonal anti-m6A (Synaptic Systems #202-003). m6A-IP was performed in 200 µL of IP buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... Pre- and post-synapses were labeled using polyclonal guinea pig anti-VGluT1 (cat. No. 135 304, 1:500; Synaptic Systems) and polyclonal rabbit anti-Homer1 (cat ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilized and stained for c-FOS using anti-c-FOS Rat monoclonal purified IgG (Synaptic Systems, Cat. No. 226 017) followed by a Donkey anti-Rat IgG Alexa Fluor− 555 (Invitrogen A78945 ...