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Citations for Synaptic Systems :
1351 - 1400 of 1402 citations for Mouse anti Legionella pneumophila LPS F029 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 48h at 4°C while rocking with a primary antibody: guinea pig anti-Vglut2 (1:500, Synaptic Systems #135404), followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C in guinea pig anti-PV polyclonal serum (1:1000; 195 004, Synaptic Systems, Göttingen, Germany), and later in Alexa biotinylated goat anti-guinea pig 594 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were subsequently applied for 3 h at the following dilutions: anti-microtubule-associated protein 2 (MAP 2) at 1:1,000 (guinea pig polyclonal, antiserum, Synaptic Systems, Göttingen, Germany) and anti-vesicular glutamate transporter 1 (anti-vGLUT1 ...
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bioRxiv - Neuroscience 2023Quote: ... the relevant brain slices were incubated for 7 days at 4#x00B0;C with rabbit anti-cFos primary antibody (1:10000, Synaptic system, #226003), and for the mCherry staining ...
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bioRxiv - Cell Biology 2023Quote: ... Cell membranes were permeabilized with 0.5% CHAPS in DPBS and blocked before being labeled with either rabbit anti-synaptophysin (Synaptic Systems, 101 002) or mouse anti-tau (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... + 10 mg/ml heparin in PBS (PTwH solution) followed by incubation with primary antibody solution (rabbit anti-Fos, 1:1000; Synaptic Systems 226008) in 5% DMSO + 3% NDS + PTwH at 37°C for 7 d ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% bovine serum albumin dissolved in 1X PBS + 0.03% Triton) for 1 hour at room temperature with gentle shaking and then incubated with anti-IBA1 primary antibody (chicken; Synaptic Systems (234009); 1:1000 ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... total RNA or mRNA was crosslinked to a charged nylon-based membrane using UV light and was incubated with primary rabbit anti-m6A antibody (Synaptic Systems, 202003) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: rabbit polyclonal anti-Ca2+ channel P/Q-type alpha-1A antibody (1:400 dilution, Synaptic Systems 152203); rabbit monoclonal anti-phospho-CaMKII (Thr286 ...
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bioRxiv - Neuroscience 2021Quote: Tissue sections were washed three times in 0.1 M PBS and then transferred to a primary antibody solution containing 1:500 rabbit anti-c-Fos antibody (226003, Synaptic Systems, Gottingen, Germany), 4% normal goat serum ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.1M PBS before being incubated in a primary antibody solution of 1:2000 rabbit anti-c-Fos primary antibody (226 003, Synaptic Systems, Göttingen, Germany), 3% normal donkey serum ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were incubated at room temperature for 2 days in a 1/15000 dilution of rabbit-anti-cFos (Synaptic Systems, Göttingen, Germany) primary antibody in diluent containing 2% NGS ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were blocked with 2-5% non-fat milk or BSA in TBS-Tween 20 (0.2%) and incubated with primary antibodies with the following dilutions: anti-Hsp90α (1:2000; antibodies from Synaptic Systems and Enzo Lifesciences), anti-Hsp90β (1:2000) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated 48 hrs at 4°C with the following primary antibodies: rabbit anti-cFos (1:1000, Synaptic Systems, #226 003), rabbit anti-TH (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... We incubated sections overnight at 4°C with a primary c-Fos antibody in blocking buffer (1:5000, rabbit anti-c-Fos, Synaptic Systems, 226 003), a primary FosB/ΔFosB antibody (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 37°C overnight (16-18 hr) with rabbit anti-Ribeye A domain (1:200, Synaptic Systems Cat# 192 103, RRID:AB_2086775) and mouse anti-GluR2 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with shaking in primary antibodies diluted in milk (1:1000 Rabbit-anti-Neuroligin2 (Synaptic Systems 129-202), or 1:200 Mouse-anti-HA-tag (supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were then incubated for 1h at room temperature with primary antibodies in blocking buffer (1:500 Rabbit anti-NL2 (Synaptic Systems, 129-203), or 1:400 Mouse anti-MPM2 (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... 1:1000 for IF) and the anti-MAP2 Polyclonal Guinea Pig Antibody (188 004, 1:1000 for IF) were purchased from Synaptic Systems (Goettingen, Germany). The anti-Tyrosine Hydroxylase Antibody (AB9702 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 % Triton X-100 % and 0.2 % BSA in PBS) containing the primary antibody (polyclonal rabbit anti-TH, 1:1000, Synaptic Systems Cat# 213 104) overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then washed 3 times (10min each) and finally incubated overnight at 4°C with primary antibody solution containing anti-parvalbumin (Parvalbumin antibody, 195004, Synaptic System 1:1000) 1% BSA and 0.1% Triton in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The washed tissue were then moved into new 1.5 ml centrifuge tubes for primary antibody staining (usually diluted 1:100 – 1:500) with: chicken anti-Homer (160006, Synaptic Systems, Goettngen, Germany), guinea pig anti-Bassoon (#141004 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 HZ in the presence of 1:100 anti VGAT luminal domain polyclonal rabbit antibody conjugated to Alexa Fluor 488 (Synaptic Systems 131 103C2). Following washout ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated with a rat anti-cFos primary antibody (1:1000 dilution in 1% BSA in PBST, Synaptic Systems, 226 017) for 24 hours at room temperature on a shaker ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNA at 6 μg were added to 10 μl Slurry of PierceTM Protein A Agarose beads pre-incubated with 1 μg of an anti-m6A polyclonal antibody (Synaptic Systems, 202-003) by rocking at 4°C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were then incubated in the blocking solution containing the guinea pig anti-c-Fos primary antibody (1:1000; Cat No. 226-308, Synaptic Systems; Gottingen, Germany) overnight at 4°C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... RIP-seq was performed according to the “Refined RIP-seq” protocol for low input material (40) with the following specifications: 2 µg anti-m6A antibody (Synaptic Systems 202 003) was conjugated to magnetic beads and incubated with 6 ug total RNA that was previously fragmented to ∼300 nt fragments with 10 mM ZnCl2 in 10 mM Tris-HCl for 4 minutes at 70°C (5% of fragmented RNA was set aside as input) ...
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bioRxiv - Biochemistry 2023Quote: ... BSA in PBS for 1 hour at RT and incubated overnight at 4 °C with primary anti-m6A antibody (Synaptic systems, 202-003) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used with 4% paraformaldehyde/4% sucrose fixation include Synapsin I/II (anti-guinea pig; Synaptic Systems: 106 004; 1:1000 dilution), PSD-95 (anti-rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at RT with one of the following primary antibodies: guinea pig anti-c-Fos (1:2000, Synaptic Systems, Cat#226004), rabbit anti-CGRP (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... with one or a mix of the following primary antibodies: Rat anti-c-Fos (1:1000, Cat. No. 226 017, Synaptic Systems, RRID: AB_2864765), Rabbit anti-c-Fos (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was incubated overnight at 4°C in primary antibody diluted in blocking solution [rabbit anti-cFos 1:1000 (Synaptic Systems, Cat#20079)] ...
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bioRxiv - Cell Biology 2024Quote: ... 10% of RNA fragments were saved as “Input,” and the remaining fragments were used for m6A immunoprecipitation (IP) using a specific anti-m6A antibody (Synaptic Systems, 202203, Germany). RNA samples from both input and IP were prepared using TRIzol reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2015) using 10 μg of purified mRNA from Drosophila S2R+ cells and 5 μg of anti-m6A antibody (Synaptic Systems, Lot# 202003/2-82). Immunoprecipitations were performed in quadruplicates and as a control one immunoprecipitation was performed where UV-crosslinking was ommited ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Sections were incubated for 48 h at 4 °C with gentle agitation with guinea pig polyclonal anti Arc (156 004; 1:1500; Synaptic Systems®, Goettingen, Germany), mouse monoclonal anti GluN1 antibody (32-0500 ...
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bioRxiv - Genetics 2020Quote: ... Membranes were washed three times in TBST and incubated for 1 h at 22–24°C using rabbit anti-Piccolo (Synaptic Systems cat. no. 142002) diluted 1:2000 in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were rinsed in TBST and cut to be incubated for 1 h at room temperature in 0.5% non-fat dried milk in TBST containing the appropriate primary antibody anti-SP (rabbit polyclonal, Synaptic Systems, 163 002, 1:1000), anti-Beta-actin (mouse monoclonal ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 72 hrs at 4°C in primary rabbit anti-vesicular acetylcholine transporter (VAChT) antisera (Synaptic Systems; 1:500, RRID_AB887864) containing 2% BSA and 0.1% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at room temperature in Iba1anti-rabbit (1:1500, Wako, product code: 019-19741) and GFAP anti-guinea pig (1:1500, Synaptic Systems, product code: 173004) as well as a marker for neurons (NeuN anti-chicken ...
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bioRxiv - Neuroscience 2020Quote: ... we used a standard free-floating immunofluorescence protocol and incubated the ACC and MCC sections overnight in S100B anti-guinea pig (1:1000, Synaptic Systems, product code: 287004) together with Serping1 anti-mouse (Santa Cruz ...
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bioRxiv - Neuroscience 2022Quote: ... Inhibitory presynaptic sites were detected by immunolabeling against vesicular GABA transporter (vGAT) using rabbit anti-vGAT antibody (1:100, Synaptic Systems, catalog # 131002, RRID:AB_887871). Inhibitory postsynaptic sites were detected by immunolabeling against gephyrin using mouse anti-gephyrin antibody (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... pyramidal neurons were morphologically identified based on the side and shape of cell body as observed using anti-MAP2 guinea pig (Synaptic Systems, #188 004; 1:1,000) and anti-GFP mouse (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated in rabbit anti-c-Fos antibody (1:4000 in 3% NGS + 0.1% Triton; Synaptic Systems, Gottingen, Germany; Lot# 226003/3-45) for 16 h at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated for 1 day at 4 °C in blocking solution with following antibodies: rabbit anti-cFos (226003, Synaptic Systems; Goettingen, GERMANY, 1:1000); goat anti-Wheat Germ Agglutinin (WGA ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices then underwent a double immunostaining for EYFP and cFos using a primary antibody cocktail of rabbit anti-cFos (1:2000, Synaptic Systems, Cat. No. 226 008) and goat anti-GFP (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary antibodies diluted in 3% NDS blocking solution and incubated at 4 °C for 72 hours (GFP anti-chicken, 1:1,000, Aves Labs, catalog no. GFP-1020; NeuN guinea pig, 1:500, Synaptic Systems, catalog no. 266 004). All sections were rinsed three times for 15 min in PBS-T and incubated for 4 hours at ∼20 °C in secondary antibodies in the blocking solution (Cy3 donkey anti-guinea pig ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.3% Tween-20 PBS for 1 h then incubated in rabbit anti-c-Fos antibody (1:500, Synaptic Systems, catalog # 226-003, RRID: AB_2231974) diluted in the same solution for 72 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked with 10% normal donkey serum in 1xPBS-T (0.5% Triton X-100) for 1 h at room temperature and then probed overnight with rabbit anti-VGAT antibody (Synaptic Systems, cat# 131 003, 1:500) in blocking solution at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... in 0.3% Triton X-100 for 2 hours before being incubated in rabbit anti-c-Fos antibody (1:4000 in 3% NGS + 0.1% Triton; Synaptic Systems, Goettingen, Germany; Lot# 226003/3-45) for 16 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... DNA-PAINT samples were incubated for 1 hour at room temperature with an anti-bassoon guinea pig antibody (Synaptic Systems cat. 141 004, 1:1000 dilution) as counterstaining and ...