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Citations for Synaptic Systems :
1351 - 1400 of 1421 citations for Goat anti guinea pig IgG 550 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... at 40um thickness from postmortem human brain blocked for 1 hour at room temperature in blocking buffer before being incubated in primary antibodies for 72 hours at 4°C with gentle rocking in blocking buffer (Anti-NeuN, Synaptic Systems cat no ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse PDI antibody (ADI-SPA-891, RRID:AB_10615355) was purchased from Enzolifesciences (Farmingdale, NY, USA). Anti-Sec22b antibody (Cat. 186003) was purchased from Synaptic Systems (Goettingen, Germany). Anti-GM130 antibody (Cat# ab52649 ...
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bioRxiv - Physiology 2022Quote: ... amino acids 2–115 on rat VGAT) was validated by co-staining with anti-VGAT mouse antibody (1:200; 131011, Synaptic Systems; epitope ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was then incubated overnight in 5 % milk TBS-T containing mouse anti-GluA1 (1:1000: Synaptic Systems, Cat# 182011) and mouse anti-GAPDH (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Inhibitory postsynaptic sites were detected by immunolabeling against gephyrin using mouse anti-gephyrin antibody (1:500, Synaptic Systems, catalog # 147111, RRID:AB_887719). Secondary antibodies used were as follows ...
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bioRxiv - Neuroscience 2023Quote: ... Then the cells were fixed with 4% PFA and stained with the following antibodies (1: 500 dilutions used for all): rabbit anti-m6A (202 003, Synaptic Systems), rabbit anti-METTL3 (15073-1-AP ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were diluted in TBS ++ (TBS with 0,1% Triton-X 100 and 6% normal donkey serum): anti-S100b (Rabbit, 1:1000; Synaptic systems, #287003) and anti-Ki67 (Rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were subsequently incubated overnight at 4°C under agitation with the primary antibodies: anti-tyrosine hydroxylase (1:500, Synaptic System) and anti-DNA (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia and astrocytes were labeled using polyclonal rabbit anti-Iba-1 (cat. No. 234 003, 1:1000; Synaptic Systems, Göttingen, Germany) and anti-GFAP antibody (cat ...
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bioRxiv - Bioengineering 2023Quote: ... Later the cultures were incubated at 4 °C with rabbit anti-cFos (Cat# 226 008, RRID:AB 2891278, Synaptic Systems, 1 : 1000) in PBS with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C in blocking solution containing primary antibody: rabbit anti-c-Fos (1:3000; Synaptic Systems). The following day ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was then incubated overnight with anti-m6A antibody (Abcam, catalog no. Ab151230; or Synaptic Systems, catalog no. 202 003) at a 1:250 dilution in 5% non-fat milk prepared in 1X PBS containing 0.02% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... with the primary antibody diluted in TBST solution containing 0,5% dry milk: rabbit anti-NLGN1 (Synaptic Systems, #129-013, 1:1000); rabbit anti-NLGN2 (Synaptic Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μL of fragmented mRNAs was taken as the input control and 100 μL were incubated with 5 µg of anti-m6A polyclonal antibody (Synaptic Systems, 202003) at 4 °C for 2 h in 450 µL of IP buffer containing 10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... GABAergic synapses were also identified by live immunolabelling of the presynaptic marker vGAT using the anti-vGAT-Oyster550 antibody (Synaptic Systems, Germany) which is directed against the luminal part of the protein ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% (v/v) Triton X-100 containing PBS at 4°C overnight with rabbit anti-synaptopodin (Synaptic Systems, #163002; 1:1000 dilution) or rabbit anti-NeuN (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Then the section was incubated for 60 h at 4°C with a 1:200 dilution of a rabbit anti MAP-2 primary antibody (188 003, Synaptic Systems, Germany) and for 3 h with a 1:250 dilution of a goat anti-rabbit secondary antibody conjugated to DyLight 680 fluorophore (35569 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% Triton X-100) and then for 20h with anti-c-fos primary rabbit antibody (1:500, 226.003; Synaptic Systems, Göttingen, Germany) for 24 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated in 600 ul total volume of TBS-0.05% NP-40 with 15 ug of mouse anti U1-70K (Synaptic systems, 203 0111), mouse anti-SmB/B’ (Y12) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100) and then for 20 hrs with anti-cfos primary rabbit antibody (1:500, 226.003; Synaptic Systems, Göttingen, Germany). Sections were washed and incubated with anti-rabbit Alexa-488 secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and the sections were incubated with a primary antibody solution (rabbit anti cFos (1:800, cat. No. 226003, Synaptic Systems, Göttingen, Germany)) containing 3% NDS in PBS for ∼17 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... incubated first with the primary antibody (microglia/macrophage specific polyclonal rabbit anti-IBA1 antibody 1:2000, 234 003, Synaptic Systems, Göttingen, Germany) at 4 °C for 18 h and subsequently with the secondary antibody (AlexaFluor 568 goat-anti-rabbit 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... 10µg/ml heparin in PBS) and placed in primary solution of rabbit anti-Fos (226 003, Synaptic Systems,Goettingen, Germany, 1:1000) and/or rabbit anti-RFP (600-401-379 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell membranes were permeabilized with 0.5% CHAPS in DPBS and blocked before being labeled with either rabbit anti-synaptophysin (Synaptic Systems, 101 002) or mouse anti-tau (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C with monoclonal rabbit anti c-Fos (1:8000, catalog #226 008, Synaptic Systems; RRID: AB_2891278) and monoclonal rat anti-mCherry (1:3000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 48 hours in rabbit anti-c-Fos primary antibody (1:1000 in blocking solution, 226_003, waiting on RRID approval; Synaptic Systems, Goettingen, Germany). Next ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C with the primary mouse anti-vesicular glutamate transporter 1 (VGLUT1) antibody (1:200, Synaptic Systems, RRID:AB_887879) to analyze presynaptic excitatory punctae and the guinea pig anti-vesicular GABA transporter (VGAT ...
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bioRxiv - Neuroscience 2023Quote: ... the relevant brain slices were incubated for 7 days at 4#x00B0;C with rabbit anti-cFos primary antibody (1:10000, Synaptic system, #226003), and for the mCherry staining ...
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bioRxiv - Physiology 2023Quote: ... The second c-fos antibody used was a monoclonal rat anti-c-fos antibody (1:250, Synaptic Systems, catalog #226017, RRID: AB_2864765). Similar to the above-mentioned c-fos antibody ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: rabbit polyclonal anti-Ca2+ channel P/Q-type alpha-1A antibody (1:400 dilution, Synaptic Systems 152203); rabbit monoclonal anti-phospho-CaMKII (Thr286 ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% bovine serum albumin dissolved in 1X PBS + 0.03% Triton) for 1 hour at room temperature with gentle shaking and then incubated with anti-IBA1 primary antibody (chicken; Synaptic Systems (234009); 1:1000 ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... total RNA or mRNA was crosslinked to a charged nylon-based membrane using UV light and was incubated with primary rabbit anti-m6A antibody (Synaptic Systems, 202003) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... + 10 mg/ml heparin in PBS (PTwH solution) followed by incubation with primary antibody solution (rabbit anti-Fos, 1:1000; Synaptic Systems 226008) in 5% DMSO + 3% NDS + PTwH at 37°C for 7 d ...
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bioRxiv - Neuroscience 2021Quote: Tissue sections were washed three times in 0.1 M PBS and then transferred to a primary antibody solution containing 1:500 rabbit anti-c-Fos antibody (226003, Synaptic Systems, Gottingen, Germany), 4% normal goat serum ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.1M PBS before being incubated in a primary antibody solution of 1:2000 rabbit anti-c-Fos primary antibody (226 003, Synaptic Systems, Göttingen, Germany), 3% normal donkey serum ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were incubated at room temperature for 2 days in a 1/15000 dilution of rabbit-anti-cFos (Synaptic Systems, Göttingen, Germany) primary antibody in diluent containing 2% NGS ...
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bioRxiv - Neuroscience 2020Quote: ... perineuronal nets were revealed by incubating the neurons with 1:500 mouse anti-TNR antibodies (#217 011; clone 619; Synaptic Systems, Göttingen, Germany) or 1:500 biotinylated Wisteria floribunda agglutinin WFA (#L8258 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were blocked with 2-5% non-fat milk or BSA in TBS-Tween 20 (0.2%) and incubated with primary antibodies with the following dilutions: anti-Hsp90α (1:2000; antibodies from Synaptic Systems and Enzo Lifesciences), anti-Hsp90β (1:2000) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated 48 hrs at 4°C with the following primary antibodies: rabbit anti-cFos (1:1000, Synaptic Systems, #226 003), rabbit anti-TH (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... We incubated sections overnight at 4°C with a primary c-Fos antibody in blocking buffer (1:5000, rabbit anti-c-Fos, Synaptic Systems, 226 003), a primary FosB/ΔFosB antibody (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 37°C overnight (16-18 hr) with rabbit anti-Ribeye A domain (1:200, Synaptic Systems Cat# 192 103, RRID:AB_2086775) and mouse anti-GluR2 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with shaking in primary antibodies diluted in milk (1:1000 Rabbit-anti-Neuroligin2 (Synaptic Systems 129-202), or 1:200 Mouse-anti-HA-tag (supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were then incubated for 1h at room temperature with primary antibodies in blocking buffer (1:500 Rabbit anti-NL2 (Synaptic Systems, 129-203), or 1:400 Mouse anti-MPM2 (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 % Triton X-100 % and 0.2 % BSA in PBS) containing the primary antibody (polyclonal rabbit anti-TH, 1:1000, Synaptic Systems Cat# 213 104) overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then washed 3 times (10min each) and finally incubated overnight at 4°C with primary antibody solution containing anti-parvalbumin (Parvalbumin antibody, 195004, Synaptic System 1:1000) 1% BSA and 0.1% Triton in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The washed tissue were then moved into new 1.5 ml centrifuge tubes for primary antibody staining (usually diluted 1:100 – 1:500) with: chicken anti-Homer (160006, Synaptic Systems, Goettngen, Germany), guinea pig anti-Bassoon (#141004 ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated with a rat anti-cFos primary antibody (1:1000 dilution in 1% BSA in PBST, Synaptic Systems, 226 017) for 24 hours at room temperature on a shaker ...
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bioRxiv - Biochemistry 2023Quote: ... BSA in PBS for 1 hour at RT and incubated overnight at 4 °C with primary anti-m6A antibody (Synaptic systems, 202-003) diluted in the blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNA at 6 μg were added to 10 μl Slurry of PierceTM Protein A Agarose beads pre-incubated with 1 μg of an anti-m6A polyclonal antibody (Synaptic Systems, 202-003) by rocking at 4°C for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... RIP-seq was performed according to the “Refined RIP-seq” protocol for low input material (40) with the following specifications: 2 µg anti-m6A antibody (Synaptic Systems 202 003) was conjugated to magnetic beads and incubated with 6 ug total RNA that was previously fragmented to ∼300 nt fragments with 10 mM ZnCl2 in 10 mM Tris-HCl for 4 minutes at 70°C (5% of fragmented RNA was set aside as input) ...