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• Pentameric assembly of glycine receptor intracellular domains provides insights into gephyrin clustering

  Arthur Macha, et al., bioRxiv - Biochemistry 2022

  Quote: ... Primary antibodies anti-gephyrin (1:50, cell culture supernatant, clone 3B11, Synaptic Systems) and anti-GABAAR subunit α2 (1:50 ...
• A selectivity filter in the EMC limits protein mislocalization to the ER

  Tino Pleiner, et al., bioRxiv - Cell Biology 2022

  Quote: ... CAML (Synaptic Systems, Germany); Anti-HA-HRP (Millipore-Sigma ...
• Single Cell transcriptional analysis of ex vivo models of cortical and hippocampal development identifies unique longitudinal trends

  Daniel K. Krizay, et al., bioRxiv - Genetics 2022

  Quote: ... Primary antibodies: Synapsin 1 (SYN) antibody (106 011, Synaptic Systems, 1:1000, mouse), Glial Fibrillary Acidic Protein (GFAP ...
• TMPRSS2 is essential for SARS-CoV-2 Beta and Omicron infection

  Kristin Metzdorf, et al., bioRxiv - Immunology 2022

  Quote: ... 3 μm thick FFPE sections were stained for SARS-CoV-2 with mouse-anti-nucleocapsid CoV-1/2 (Synaptic Systems, HS-452 11, clone 53E2, subtype: IgG2a) and for macrophages with rat-anti-mouse-MAC2 (Biozol Diagnostica/CEDARLANE,CL8942AP ...
• Engineered retrovirus-like nanocarriers for messenger RNA delivery into neurons

  Wenchao Gu, et al., bioRxiv - Bioengineering 2022

  Quote: ... an Arc antibody (Synaptic systems 156 003) was used to immunoprecipitate the Arc capsids carrying the mRNA cargo inside ...
• A Parabrachial to Hypothalamic Pathway Mediates Defensive Behavior

  Fan Wang, et al., bioRxiv - Neuroscience 2022

  Quote: ... The primary antibodies used were: Guinea pig anti-c-fos (Synaptic Systems, Cat# 226004), Rabbit anti-Dsred (Takara ...
• Perturbation of the mitochondrial import machinery by aggregation prone Tau affects organelle morphology and reduces neuronal complexity

  Hope I Needs, et al., bioRxiv - Cell Biology 2022

  Quote: ... Gephyrin (Synaptic Systems; 147111), and Synaptophysin (Merck Millipore ...
• Spatial modeling of telomere intra-nuclear distribution reveals non-random organization that varies during cell cycle and depends on LAP2 and BAF

  Debora Keller, et al., bioRxiv - Cell Biology 2022

  Quote: ... 2.5 μg m6A antibody (Synaptic Systems) and rotated overnight at 4°C ...
• Spatial modeling of telomere intra-nuclear distribution reveals non-random organization that varies during cell cycle and depends on LAP2 and BAF

  Debora Keller, et al., bioRxiv - Cell Biology 2022

  Quote: ... m6A antibody (Synaptic Systems) was diluted to 1:2000 in 5% non-fat dry milk and 0.1% TBST ...
• Human mutations in SLITRK3 implicated in GABAergic synapse development in mice

  Stephanie Efthymiou, et al., bioRxiv - Genetics 2022

  Quote: ... anti-Gephyrin (1:500, 147018, Synaptic Systems), and anti-vGAT (1:500 ...
• Single Cell transcriptional analysis of ex vivo models of cortical and hippocampal development identifies unique longitudinal trends

  Daniel K. Krizay, et al., bioRxiv - Genetics 2022

  Quote: ... MAP2 antibody (188 004, Synaptic Systems, 1:1000, guinea pig). Secondary antibodies ...
• Arc ubiquitination regulates endoplasmic reticulum-mediated Ca²⁺ release and CaMKII signaling

  Mohammad A. Ghane, et al., bioRxiv - Neuroscience 2022

  Quote: ... Cells were then incubated with primary antibodies (rabbit anti-Arc (Synaptic Systems #156003; 1:500); rabbit anti-CaMKIIα/β (Abcam #ab52476 ...
• Arc ubiquitination regulates endoplasmic reticulum-mediated Ca²⁺ release and CaMKII signaling

  Mohammad A. Ghane, et al., bioRxiv - Neuroscience 2022

  Quote: ... overnight at 4°C and then incubated with primary antibodies (Arc, Synaptic Systems #156003 ...
• A Purkinje cell to parabrachial nucleus pathway enables broad cerebellar influence over the forebrain

  Christopher H. Chen, et al., bioRxiv - Neuroscience 2022

  Quote: ... Sections were stained for vGAT (Synaptic Systems, #131 004, 1:500) and tyrosine hydroxylase to identify LC neurons (Immunostar ...
• Neurexins in serotonergic neurons regulate serotonin transmission and complex mouse behaviors

  Amy Cheung, et al., bioRxiv - Neuroscience 2022

  Quote: ... RIM1/2 (Rabbit, Synaptic Systems, 140203, RRID: AB_887775, 1:1000), synaptophysin (guinea pig ...
• Long-term potentiation in neurogliaform cells modulates excitation-inhibition balance in the temporoammonic pathway

  Marion S. Mercier, et al., bioRxiv - Neuroscience 2021

  Quote: ... 0.5% BSA and guinea pig anti-GFP antibody (1:1000; Synaptic Systems, 132 005) and left overnight at 4°C ...
• Versatile, do-it-yourself, low-cost spinning disk confocal microscope

  Aaron R. Halpern, et al., bioRxiv - Biophysics 2021

  Quote: ... rabbit anti-homer1 (Synaptic Systems, 160003) and mouse anti-bassoon (Abcam ...
• Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release

  Benoît Delignat-Lavaud, et al., bioRxiv - Neuroscience 2021

  Quote: ... and rabbit anti-Syt4 or anti-Syt7 primary antibodies (Synaptic Systems; 1:1000) subsequently detected using Alexa Fluor-488-conjugated and Alexa Fluor-546-conjugated secondary antibodies (Invitrogen ...
• Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release

  Benoît Delignat-Lavaud, et al., bioRxiv - Neuroscience 2021

  Quote: ... and incubated overnight at room temperature with the first primary antibody (rabbit anti-Syt1, anti-Syt4 or anti Syt7 from Synaptic Systems, Germany; 1:1000), followed by 2h with a first secondary antibody (rabbit Alexa Fluor-488–conjugated ...
• Single-cell RNA transcriptome analysis reveals CXCL16/CXCR6 as maintenance factors for CNS tissue resident T cells that drive synapse elimination during viral recovery

  Sarah F. Rosen, et al., bioRxiv - Immunology 2021

  Quote: ... IBA1 (1:200, Synaptic Systems, cat. no. 234 006), IL-1β (1:100 ...
• Single-cell RNA transcriptome analysis reveals CXCL16/CXCR6 as maintenance factors for CNS tissue resident T cells that drive synapse elimination during viral recovery

  Sarah F. Rosen, et al., bioRxiv - Immunology 2021

  Quote: ... synaptophysin (1:250; Synaptic Systems, cat. no. 101004, polyclonal), CD68 (1:200 ...
• Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKIIα-Cre mouse line

  Ken Matsuura, et al., bioRxiv - Genetics 2021

  Quote: ... Antibodies used were rabbit polyclonal antibody (RpAb) to Syt1 (1:1000) from Synaptic Systems (105103), mouse monoclonal antibody (MmAb ...
• N⁶-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner

  Leilei Zhou, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 5 μL of fragmented mRNAs was taken as the input control and 100 μL were incubated with 5 µg of anti-m6A polyclonal antibody (Synaptic Systems, 202003) at 4 °C for 2 h in 450 µL of IP buffer containing 10 mM Tris-HCl ...
• Single-cell transcriptomic analysis unveils spinal motor neuron subtype diversity underpinning the water-to-land transition in vertebrates

  Ee Shan Liau, et al., bioRxiv - Developmental Biology 2021

  Quote: ... guinea pig anti-Satb2 (1:1000 Synaptic Systems Cat# 327004, RRID:AB_2620070); rat anti-Bcl11b/Ctip2 (1:1000 Abcam Cat# ab18465 ...
• Cue-specific remodeling of the neuronal transcriptome through intron retention programs

  Maxime Mazille, Peter Scheiffele, Oriane Mauger, bioRxiv - Neuroscience 2021

  Quote: ... anti-MAP2 (Synaptic systems, 188004), Cy5-conjugated donkey anti-guinea pig (Jackson ...
• Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses

  Tania López-Hernández, et al., bioRxiv - Neuroscience 2021

  Quote: ... labelling of hippocampal neurons with a cypHer-conjugated antibody directed against the luminal domain of VGAT (#131103CpH; Synaptic Systems) was performed by incubating primary neurons with anti-VGAT cypHer at 1:120 (from a 1 mg/ml stock ...
• Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD

  Luisa Speranza, et al., bioRxiv - Neuroscience 2021

  Quote: ... guinea pig anti-Synaptopodin (1/500; Synaptic Systems, RRID: AB_10549419) chicken anti-MAP2 (1/500 ...
• Intermediary role of lung alveolar type 1 cells in epithelial repair upon Sendai virus infection

  Belinda J Hernandez, et al., bioRxiv - Developmental Biology 2021

  Quote: ... guinea pig anti-lysosomal-associated membrane protein 3 (LAMP3, 1:500, Synaptic Systems, 391005), rat anti-advanced glycosylation end product-specific receptor (RAGE ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... antibodies against parvalbumin (1:500, Synaptic Systems), gephyrin (1:250 ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... together with anti-parvalbumin primary antibody (1:500, Synaptic Systems, Goettigen, Germany) were used ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... and vGAT (1:200, inh. Presynaptic, Synaptic Systems) were used ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... bassoon (1:250, Excyt. presynaptic active zone, Synaptic Systems) and vGAT (1:200 ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... vGAT (1:1000, Synaptic Systems), SNAP25 (1:1000 ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... gephyrin (1:250, inh. Postsynaptic, Synaptic Systems), bassoon (1:250 ...
• Perineuronal nets affect memory and learning after synapse withdrawal

  Jiri Ruzicka, et al., bioRxiv - Neuroscience 2021

  Quote: ... SNAP25 (1:1000, Synaptic Systems). Secondary antibodies Goat anti rabbit-HRP (1:15000 ...
• N⁶-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner

  Leilei Zhou, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 5 µg of fragmented mRNAs was mixed with 10 µg of anti-m6A polyclonal antibody (Synaptic Systems, 202003) and incubated at 4 °C for 2 h in 450 µ L of IP buffer consisting of 10 mM Tris-HCl ...
• Dual expression of Atoh1 and Ikzf2 promotes transformation of adult cochlear supporting cells into outer hair cells

  Suhong Sun, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and anti-vGlut3 (rabbit, 1:500, 135203, Synaptic System). Cochlear tissues were counterstained with Hoechst 33342 solution in PBST (1:1000 ...
• Synaptic Activity-Dependent Changes in the Hippocampal Palmitoylome

  Glory Nasseri, et al., bioRxiv - Neuroscience 2021

  Quote: ... Syntaxin-1a (1:500; Synaptic Systems 132 002), VGLUT1 (1:1,000 ...
• IL-37 expression reduces acute and chronic neuroinflammation and rescues cognitive impairment in an Alzheimer’s disease mouse model

  Niklas Lonnemann, et al., bioRxiv - Neuroscience 2021

  Quote: ... Slices were incubated overnight at 4°C with anti-ionized calcium-binding adaptor molecule 1 (IBA-1) (1:1000; rabbit polyclonal, Synaptic System) primary antibody diluted in 1X PBS ...
• Probing the segregation of evoked and spontaneous neurotransmission via photobleaching and recovery of a fluorescent glutamate sensor

  Camille S. Wang, et al., bioRxiv - Neuroscience 2021

  Quote: ... Primary antibody against GFP (rabbit, Synaptic Systems, 1:300) was added to detect iGluSnFR molecules overnight ...
• Deficiency in endocannabinoid synthase DAGLB contributes to Parkinson’s disease and dopaminergic neuron dysfunction

  Zhenhua Liu, et al., bioRxiv - Neuroscience 2021

  Quote: ... mouse monoclonal anti-CB1 (Synaptic systems, 258011, 1:500), rabbit monoclonal anti-TH (Pel-Freez Biologicals ...
• The m⁶A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells

  Fugui Niu, et al., bioRxiv - Neuroscience 2021

  Quote: ... m6A antibody (Synaptic Systems, 202003) and corresponding control IgG were used in this experiment ...
• BDNF-dependent modulation of axonal transport is selectively impaired in ALS

  Andrew P. Tosolini, et al., bioRxiv - Neuroscience 2021

  Quote: ... TUJ1 (Synaptic systems, 302306, synaptophysin, 1:500), TrkB (Millipore ...
• Disrupting ATXN1 Nuclear Localization in a Knock-in SCA1 Mouse Model Improves a Spectrum of SCA1-Like Phenotypes and their Brain Region Associated Transcriptomic Profiles

  Hillary P. Handler, et al., bioRxiv - Neuroscience 2021

  Quote: ... 1:250 anti-guinea pig CALB1 (Synaptic Systems)) at 4°C ...
• Inhibitory hippocampo-septal projection controls locomotion and exploratory behavior

  Yuh-Tarng Chen, Jun Guo, Wei Xu, bioRxiv - Neuroscience 2021

  Quote: ... sections were incubated overnight in primary antibodies (anti-cFos antibody: 1:1000, catalog # 226 003, Synaptic Systems (SYSY)) with 1% horse serum ...
• Myelin speeds cortical oscillations by consolidating phasic parvalbumin-mediated inhibition

  Mohit Dubey, et al., bioRxiv - Neuroscience 2021

  Quote: ... mouse anti-PV (1:1000; Swant) rabbit anti-syt2 (1:500, Synaptic Systems) in PBS blocking solution containing 5% BSA with 5% NGS and 0.3% Triton-X ...
• Synaptic and intrinsic mechanisms impair reticular thalamus and thalamocortical neuron function in a Dravet syndrome mouse model

  Carleigh Studtmann, et al., bioRxiv - Neuroscience 2021

  Quote: ... and immunostained with antibodies against: VGLUT1 (1:100; Synaptic Systems; 135-511), VGLUT2 (1:200 ...
• Off-target expression of Cre-dependent adeno-associated viruses in wild type C57BL/6J mice

  Justin J. Botterill, et al., bioRxiv - Neuroscience 2021

  Quote: ... Sections were then incubated overnight at 4 °C with polyclonal rabbit anti-cFos (#226 003, Synaptic Systems, RRID: AB_2231974) and monoclonal rat anti-mCherry (#M11217 ...
• Fast resupply of synaptic vesicles requires Synaptotagmin-3

  Dennis J. Weingarten, et al., bioRxiv - Neuroscience 2021

  Quote: ... then incubated overnight at 4 °C in vehicle with primary antibodies (rabbit anti-Syt3, 1:500, Synaptic Systems 105133; guinea pig anti-vGlut1, 1:500, Synaptic Systems 135304 ...
• Fast resupply of synaptic vesicles requires Synaptotagmin-3

  Dennis J. Weingarten, et al., bioRxiv - Neuroscience 2021

  Quote: ... then incubated overnight at 4 °C in vehicle with primary antibodies (rabbit anti-Syt3, 1:500, Synaptic Systems 105133 ...