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Citations for Synaptic Systems :
601 - 650 of 678 citations for Fc Receptor Like Protein 3 FCRL3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Then the section was incubated for 60 h at 4°C with a 1:200 dilution of a rabbit anti MAP-2 primary antibody (188 003, Synaptic Systems, Germany) and for 3 h with a 1:250 dilution of a goat anti-rabbit secondary antibody conjugated to DyLight 680 fluorophore (35569 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals; Iba1: anti-iba1 [chicken], 1:1500 Synaptic Systems) for 48hrs at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated with primary antibodies (anti-Cre IgG1, Millipore, 1:1000; anti-NEUN, Cell Signaling, Danvers, MA, 1:1000; anti-vGLUT2, Synaptic Systems, Goettingen ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit polyclonal anti-GluK2 or anti-GluK5 antibodies (1:200) were used to label KAR subunits (Synaptic Systems: #180003 and 180103 respectively) on APP/PS1 and CA3-PS-KO hippocampal sections ...
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bioRxiv - Neuroscience 2021Quote: ... a monoclonal antibody (diluted 1:500) directed against a luminal epitope of Synaptotagmin-1 fluorescently labeled with Oyster®550 (105 103C3, Synaptic System) were added to the culture medium 30 min before fixing ...
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bioRxiv - Neuroscience 2021Quote: ... The Arc antibody (1:1000 for immunocytochemistry [ICC] and 1:2000 for WB, #156 003, RRID: AB_887694) was from Synaptic Systems (Goettingen, Germany) while the horseradish peroxidase (HRP)-conjugated FLAG (1:1000 for WB ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were used to amplify native mCitrine signal (1:1000, polyclonal guinea pig anti-GFP; 132-004; Synaptic Systems, Göttingen, Germany) and to identify astrocytes (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... and the sections were incubated with a primary antibody solution (rabbit anti cFos (1:800, cat. No. 226003, Synaptic Systems, Göttingen, Germany)) containing 3% NDS in PBS for ∼17 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: α-SYT1 (1:500) (DSHB; #mAB 48; RRID:AB_2199314) and α-SYT9 (1:500) (Synaptic Systems Cat# 105 053, RRID:AB_2199639). Secondary antibodies were goat α-mouse IgG-HRP (Biorad ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated overnight at 4 °C with the following primary antibodies: guinea pig anti-vGlut2 (1:10000, Synaptic Systems, 135404), chicken anti-GFP (1:3000 ...
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bioRxiv - Neuroscience 2023Quote: The uptake of 13C-creatine was assayed according to a conventional procedure 260 with slight modifications: the immunoisolated synaptic vesicles by 10 μg Syp antibody (101011, Synaptic Systems, Germany) were resuspended with the uptake buffer (150mM meglumine-tartrate ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with primary antibodies diluted in PBS containing 0.3% BSA: anti-VGLUT1 (1:2000, Synaptic Systems, guinea pig polyclonal, RRID:AB_887878), anti-PSD-95 (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections and primary neurons were incubated in primary antibody (Synapsin, 1:2 000, Thermo Fisher Scientific, Waltham, MA, USA, A-6442, PSD95, 1:500, Synaptic Systems, Göttingen ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C with the primary mouse anti-vesicular glutamate transporter 1 (VGLUT1) antibody (1:200, Synaptic Systems, RRID:AB_887879) to analyze presynaptic excitatory punctae and the guinea pig anti-vesicular GABA transporter (VGAT ...
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bioRxiv - Neuroscience 2023Quote: ... to analyze presynaptic excitatory punctae and the guinea pig anti-vesicular GABA transporter (VGAT) antibody (1:200, cytoplasmic domain, Synaptic Systems, RRID:AB_887871) to evaluate presynaptic inhibitory punctae ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 48 hours in rabbit anti-c-Fos primary antibody (1:1000 in blocking solution, 226_003, waiting on RRID approval; Synaptic Systems, Goettingen, Germany). Next ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 48h at 4°C while rocking with a primary antibody: guinea pig anti-Vglut2 (1:500, Synaptic Systems #135404), followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... were washed twice in coIP-solution and consecutively incubated for 1 hour at 4°C with an anti-synaptopodin antibody (rabbit anti-Synaptopodin, Synaptic Systems, #163002). Beads were carefully washed twice to remove unbound antibodies and incubated for 1 hour with the precleared supernatant (‘input’ ...
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bioRxiv - Neuroscience 2023Quote: ... the relevant brain slices were incubated for 7 days at 4#x00B0;C with rabbit anti-cFos primary antibody (1:10000, Synaptic system, #226003), and for the mCherry staining ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% bovine serum albumin dissolved in 1X PBS + 0.03% Triton) for 1 hour at room temperature with gentle shaking and then incubated with anti-IBA1 primary antibody (chicken; Synaptic Systems (234009); 1:1000 ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... total RNA or mRNA was crosslinked to a charged nylon-based membrane using UV light and was incubated with primary rabbit anti-m6A antibody (Synaptic Systems, 202003) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... sections were placed in primary antibody (cFos, 1:2000, Cell Signaling, #2250; Vglut, 1:500, Invitrogen, #PA5-119621; Vgat, 1:500, Synaptic Systems, #131-004;-Homer ...
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bioRxiv - Neuroscience 2024Quote: ... + 10 mg/ml heparin in PBS (PTwH solution) followed by incubation with primary antibody solution (rabbit anti-Fos, 1:1000; Synaptic Systems 226008) in 5% DMSO + 3% NDS + PTwH at 37°C for 7 d ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then incubated for seven days in a primary antibody solution (rabbit anti-cFos, 1:1000; Synaptic Systems #226-003) diluted in PBS containing 0.2% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... Membrane was then blocked in blocking buffer (5% milk in TBST) for 1 h at room temperature and incubated with N6-mA specific antibody (Synaptic Systems, 1:2000) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the area of manually demarcated complete active zones were also compared with those demarcated using a mixture of active zone-marker antibodies for RIM1/2 (5 µg/ml; Synaptic Systems, Göttingen, Germany), neurexin (5 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies (PV; 1:1000, Swant CS56; 1:100, Sigma-Aldrich; Biotin-WFA; 1:100, Sigma-Aldrich, Gephyrin; 1:200, Synaptic system) were incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies and their dilutions were used: rabbit anti GluN2B (Frontier Institute, 1:100) and rabbit anti synaptoporin (Synaptic Systems, 1:400). The following secondary antibodies and their dilutions were used ...
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bioRxiv - Pathology 2021Quote: ... saturated with PBS 2% BSA for 1 hour at RT and stained with primary antibodies specific for whole VAMP-2 (Synaptic System 104 211). After extensive washing with PBS 0.5% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... perineuronal nets were revealed by incubating the neurons with 1:500 mouse anti-TNR antibodies (#217 011; clone 619; Synaptic Systems, Göttingen, Germany) or 1:500 biotinylated Wisteria floribunda agglutinin WFA (#L8258 ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were incubated with 50 μg/mL unlabeled Fab fragments directed against TNR (custom-made from the same TNR antibody used in the rest of the work; Synaptic Systems, Göttingen, Germany), applied together with 1 mg/mL unlabeled anti-mouse nanobody ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were incubated for 5 min with 1:100 polyclonal rabbit antibodies directed against the lumenal domain of Syt1 conjugated to the fluorescent dye Oyster488 (#105 103C2; Synaptic Systems, Göttingen, Germany) in either plain Tyrode (stimulated condition ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 1:500 polyclonal rabbit antibodies directed against the lumenal domain of Syt1 conjugated to the fluorescent dye Oyster488 (#105 103C2; Synaptic Systems, Göttingen, Germany) during the TNR labeling step.
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bioRxiv - Neuroscience 2020Quote: ... surface TNR epitopes were blocked with 10 μg/mL unlabeled Fab fragments directed against TNR (custom-made from the same TNR antibody used in the rest of the work; Synaptic Systems, Göttingen, Germany), applied together with 1 mg/mL unlabeled anti-mouse nanobody (custom-made ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated 48 hrs at 4°C with the following primary antibodies: rabbit anti-cFos (1:1000, Synaptic Systems, #226 003), rabbit anti-TH (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... and Syt1Ab uptake in the concentration indicated as follows: rabbit antibodies against Syt1 (labelled with Oyster 550; live staining: 1:70, # 105103C3, Synaptic System, Göttingen, Germany), pThr138SNAP25 (WB:1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with shaking in primary antibodies diluted in milk (1:1000 Rabbit-anti-Neuroligin2 (Synaptic Systems 129-202), or 1:200 Mouse-anti-HA-tag (supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were then incubated for 1h at room temperature with primary antibodies in blocking buffer (1:500 Rabbit anti-NL2 (Synaptic Systems, 129-203), or 1:400 Mouse anti-MPM2 (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Nitrocellulose membranes were blocked with 5% milk in TBST buffer and used to detect m6A levels by probing with m6A specific antibodies (Synaptic Systems; 202 003). Images were taken by Amersham Biosciences Imager 600 (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... 1:1000 for IF) and the anti-MAP2 Polyclonal Guinea Pig Antibody (188 004, 1:1000 for IF) were purchased from Synaptic Systems (Goettingen, Germany). The anti-Tyrosine Hydroxylase Antibody (AB9702 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 % Triton X-100 % and 0.2 % BSA in PBS) containing the primary antibody (polyclonal rabbit anti-TH, 1:1000, Synaptic Systems Cat# 213 104) overnight at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the cryo-sections were incubated for 48 h at 4 °C (while shaking) with a solution containing mouse primary antibody against GluA1 (1:1000; Synaptic Systems, Cat# 182011) in 0.01 M PBS with 10 % NGS and 0.1 % Triton X-100.
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bioRxiv - Neuroscience 2022Quote: ... The washed tissue were then moved into new 1.5 ml centrifuge tubes for primary antibody staining (usually diluted 1:100 – 1:500) with: chicken anti-Homer (160006, Synaptic Systems, Goettngen, Germany), guinea pig anti-Bassoon (#141004 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 HZ in the presence of 1:100 anti VGAT luminal domain polyclonal rabbit antibody conjugated to Alexa Fluor 488 (Synaptic Systems 131 103C2). Following washout ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated for two nights in primary antibody (1:500 Rabbit-anti-GABAA-α1; Synaptic Systems 224 203, RRID:AB_2232180 or Rabbit-anti-vGAT, Synaptic Systems 131 002, RRID:AB_887871) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated with a rat anti-cFos primary antibody (1:1000 dilution in 1% BSA in PBST, Synaptic Systems, 226 017) for 24 hours at room temperature on a shaker ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were then incubated in the blocking solution containing the guinea pig anti-c-Fos primary antibody (1:1000; Cat No. 226-308, Synaptic Systems; Gottingen, Germany) overnight at 4°C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... RIP-seq was performed according to the “Refined RIP-seq” protocol for low input material (40) with the following specifications: 2 µg anti-m6A antibody (Synaptic Systems 202 003) was conjugated to magnetic beads and incubated with 6 ug total RNA that was previously fragmented to ∼300 nt fragments with 10 mM ZnCl2 in 10 mM Tris-HCl for 4 minutes at 70°C (5% of fragmented RNA was set aside as input) ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used with 4% paraformaldehyde/4% sucrose fixation include Synapsin I/II (anti-guinea pig; Synaptic Systems: 106 004; 1:1000 dilution), PSD-95 (anti-rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... spontaneously active synaptic boutons were labeled by incubating cells with CypHer5E-conjugated antibodies against the luminal domain of vGAT (1:500 from a 1 mg/ml stock; Synaptic Systems; Cat#131,103CpH) for 2 h in their respective conditioned culture media at 37 °C and 5 % CO2 prior to imaging.