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Citations for Synaptic Systems :
551 - 600 of 649 citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Turnover of synaptic vesicles in mature cultures (DIV 15-25) was determined by live labeling with Atto647N-labeled mouse antibody against the luminal domain of synaptotagmin-1 (Synaptic Systems, cat ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were blocked with 3% BSA in PBS with 0.3% Triton X-100 for 1h at room temperature and subsequently incubated with primary antibodies (rabbit anti-glutamate, 1:500, Sigma; rabbit anti-SOM, 1:500, Novus; rabbit anti-c-Fos, 1:500, Synaptic Systems) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked in PBS containing 5% normal donkey serum for 1 h at room temperature before overnight incubation at 4°C with the following primary antibodies: MAP2 (188006, Synaptic Systems); HOMER1 (160004 ...
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bioRxiv - Neuroscience 2023Quote: ... and 5% bovine serum albumin (BSA) for 1hr at RT followed by incubation in guinea pig anti-calbindin primary antibody (1:1000, Synaptic Systems) solution overnight (18-20hrs ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was then incubated overnight with anti-m6A antibody (Abcam, catalog no. Ab151230; or Synaptic Systems, catalog no. 202 003) at a 1:250 dilution in 5% non-fat milk prepared in 1X PBS containing 0.02% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... then blocked in 5% normal donkey serum / 0.2% triton-X PBS solution for 60 min and incubated overnight in a polyclonal rabbit anti-cfos igG primary antibody (1:2500; Synaptic Systems) and chicken polyclonal anti-GFP igY primary antibody (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... Then the cleaved RNA fragments were subjected to incubation for 2h at 4°C with m6A-specific antibody (cat. #202003, Synaptic Systems) in IP buffer (50 mM Tris- HCl ...
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bioRxiv - Neuroscience 2024Quote: ... with the primary antibody diluted in TBST solution containing 0,5% dry milk: rabbit anti-NLGN1 (Synaptic Systems, #129-013, 1:1000); rabbit anti-NLGN2 (Synaptic Systems ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970], guinea pig anti-FOS [1:1000, Synaptic Systems, 226308] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... sections (20 μm) were incubated for 48 hrs at 4 °C with primary antibody for IBA1 (1:2000; Cat# 234 308, Synaptic Systems), P2Y12R (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% milk in PBST and probed overnight at 4°C with a rabbit m6A antibody (1:250; Synaptic Systems), followed by incubation with HRP-conjugated anti-rabbit IgG secondary antibody (1:5000) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM CaCl2) and immediately incubated with an Oyster 550-labeled anti-synaptotagmin-1 rabbit antibody (Synaptic Systems, #105 103C3; 1:500) for 20 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μL of fragmented mRNAs was taken as the input control and 100 μL were incubated with 5 µg of anti-m6A polyclonal antibody (Synaptic Systems, 202003) at 4 °C for 2 h in 450 µL of IP buffer containing 10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4 °C in vehicle with primary antibodies (rabbit anti-Syt3, 1:500, Synaptic Systems 105133; guinea pig anti-vGlut1, 1:500, Synaptic Systems 135304 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in PBS-T + 5% NGS as follows: guinea pig anti-c-Fos (1:1000, Synaptic Systems, #226 004), chicken anti-GFP (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: Recycling synaptic vesicles were labelled using a mouse monoclonal antibody against the lumenal (intravesicular) domain of Synaptotagmin 1 (clone 604.2; Synaptic Systems, Göttingen, Germany) conjugated to either Sulfo-Cyanine 3 (#105 311C3 ...
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bioRxiv - Neuroscience 2021Quote: ... GABAergic synapses were also identified by live immunolabelling of the presynaptic marker vGAT using the anti-vGAT-Oyster550 antibody (Synaptic Systems, Germany) which is directed against the luminal part of the protein ...
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bioRxiv - Neuroscience 2022Quote: ... Then the section was incubated for 60 h at 4°C with a 1:200 dilution of a rabbit anti MAP-2 primary antibody (188 003, Synaptic Systems, Germany) and for 3 h with a 1:250 dilution of a goat anti-rabbit secondary antibody conjugated to DyLight 680 fluorophore (35569 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals; Iba1: anti-iba1 [chicken], 1:1500 Synaptic Systems) for 48hrs at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated with primary antibodies (anti-Cre IgG1, Millipore, 1:1000; anti-NEUN, Cell Signaling, Danvers, MA, 1:1000; anti-vGLUT2, Synaptic Systems, Goettingen ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% Triton X-100) and then for 20h with anti-c-fos primary rabbit antibody (1:500, 226.003; Synaptic Systems, Göttingen, Germany) for 24 hr ...
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bioRxiv - Neuroscience 2021Quote: ... a monoclonal antibody (diluted 1:500) directed against a luminal epitope of Synaptotagmin-1 fluorescently labeled with Oyster®550 (105 103C3, Synaptic System) were added to the culture medium 30 min before fixing ...
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bioRxiv - Neuroscience 2021Quote: ... The Arc antibody (1:1000 for immunocytochemistry [ICC] and 1:2000 for WB, #156 003, RRID: AB_887694) was from Synaptic Systems (Goettingen, Germany) while the horseradish peroxidase (HRP)-conjugated FLAG (1:1000 for WB ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was blocked again (4% BSA, 3% donkey serum, 0.1% Triton) for 1 hour before incubation in synaptobrevin-2 primary antibody (Synaptic Systems; 104 211C3) (1:200 in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 48 hours in rabbit anti-c-Fos primary antibody (1:1000 in blocking solution, 226_003, waiting on RRID approval; Synaptic Systems, Goettingen, Germany). Next ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4°C with the primary mouse anti-vesicular glutamate transporter 1 (VGLUT1) antibody (1:200, Synaptic Systems, RRID:AB_887879) to analyze presynaptic excitatory punctae and the guinea pig anti-vesicular GABA transporter (VGAT ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections and primary neurons were incubated in primary antibody (Synapsin, 1:2 000, Thermo Fisher Scientific, Waltham, MA, USA, A-6442, PSD95, 1:500, Synaptic Systems, Göttingen ...
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bioRxiv - Neuroscience 2023Quote: ... to analyze presynaptic excitatory punctae and the guinea pig anti-vesicular GABA transporter (VGAT) antibody (1:200, cytoplasmic domain, Synaptic Systems, RRID:AB_887871) to evaluate presynaptic inhibitory punctae ...
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bioRxiv - Neuroscience 2022Quote: ... and the sections were incubated with a primary antibody solution (rabbit anti cFos (1:800, cat. No. 226003, Synaptic Systems, Göttingen, Germany)) containing 3% NDS in PBS for ∼17 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: α-SYT1 (1:500) (DSHB; #mAB 48; RRID:AB_2199314) and α-SYT9 (1:500) (Synaptic Systems Cat# 105 053, RRID:AB_2199639). Secondary antibodies were goat α-mouse IgG-HRP (Biorad ...
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bioRxiv - Neuroscience 2023Quote: The uptake of 13C-creatine was assayed according to a conventional procedure 260 with slight modifications: the immunoisolated synaptic vesicles by 10 μg Syp antibody (101011, Synaptic Systems, Germany) were resuspended with the uptake buffer (150mM meglumine-tartrate ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with primary antibodies diluted in PBS containing 0.3% BSA: anti-VGLUT1 (1:2000, Synaptic Systems, guinea pig polyclonal, RRID:AB_887878), anti-PSD-95 (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated overnight at 4 °C with the following primary antibodies: guinea pig anti-vGlut2 (1:10000, Synaptic Systems, 135404), chicken anti-GFP (1:3000 ...
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bioRxiv - Neuroscience 2023Quote: ... the relevant brain slices were incubated for 7 days at 4#x00B0;C with rabbit anti-cFos primary antibody (1:10000, Synaptic system, #226003), and for the mCherry staining ...
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bioRxiv - Neuroscience 2023Quote: ... were washed twice in coIP-solution and consecutively incubated for 1 hour at 4°C with an anti-synaptopodin antibody (rabbit anti-Synaptopodin, Synaptic Systems, #163002). Beads were carefully washed twice to remove unbound antibodies and incubated for 1 hour with the precleared supernatant (‘input’ ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were used to amplify native mCitrine signal (1:1000, polyclonal guinea pig anti-GFP; 132-004; Synaptic Systems, Göttingen, Germany) and to identify astrocytes (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100) and then for 20 hrs with anti-cfos primary rabbit antibody (1:500, 226.003; Synaptic Systems, Göttingen, Germany). Sections were washed and incubated with anti-rabbit Alexa-488 secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 48h at 4°C while rocking with a primary antibody: guinea pig anti-Vglut2 (1:500, Synaptic Systems #135404), followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then incubated for seven days in a primary antibody solution (rabbit anti-cFos, 1:1000; Synaptic Systems #226-003) diluted in PBS containing 0.2% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were placed in primary antibody (cFos, 1:2000, Cell Signaling, #2250; Vglut, 1:500, Invitrogen, #PA5-119621; Vgat, 1:500, Synaptic Systems, #131-004;-Homer ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% bovine serum albumin dissolved in 1X PBS + 0.03% Triton) for 1 hour at room temperature with gentle shaking and then incubated with anti-IBA1 primary antibody (chicken; Synaptic Systems (234009); 1:1000 ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... total RNA or mRNA was crosslinked to a charged nylon-based membrane using UV light and was incubated with primary rabbit anti-m6A antibody (Synaptic Systems, 202003) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... + 10 mg/ml heparin in PBS (PTwH solution) followed by incubation with primary antibody solution (rabbit anti-Fos, 1:1000; Synaptic Systems 226008) in 5% DMSO + 3% NDS + PTwH at 37°C for 7 d ...
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bioRxiv - Neuroscience 2024Quote: ... Subsequently membranes were solubilized with CL-91 and processed as described above for APs with a mixture of anti-Nrx1-3 antibodies (anti-Nrx1, #ABN161, Millipore; anti-Nrx1, Synaptic Systems, #175103) and subjected to MS-analysis.
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bioRxiv - Neuroscience 2020Quote: ... Membrane was then blocked in blocking buffer (5% milk in TBST) for 1 h at room temperature and incubated with N6-mA specific antibody (Synaptic Systems, 1:2000) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the area of manually demarcated complete active zones were also compared with those demarcated using a mixture of active zone-marker antibodies for RIM1/2 (5 µg/ml; Synaptic Systems, Göttingen, Germany), neurexin (5 µg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies and their dilutions were used: rabbit anti GluN2B (Frontier Institute, 1:100) and rabbit anti synaptoporin (Synaptic Systems, 1:400). The following secondary antibodies and their dilutions were used ...
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bioRxiv - Pathology 2021Quote: ... saturated with PBS 2% BSA for 1 hour at RT and stained with primary antibodies specific for whole VAMP-2 (Synaptic System 104 211). After extensive washing with PBS 0.5% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... perineuronal nets were revealed by incubating the neurons with 1:500 mouse anti-TNR antibodies (#217 011; clone 619; Synaptic Systems, Göttingen, Germany) or 1:500 biotinylated Wisteria floribunda agglutinin WFA (#L8258 ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were incubated with 50 μg/mL unlabeled Fab fragments directed against TNR (custom-made from the same TNR antibody used in the rest of the work; Synaptic Systems, Göttingen, Germany), applied together with 1 mg/mL unlabeled anti-mouse nanobody ...