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Citations for Synaptic Systems :
551 - 593 of 593 citations for OsMPK1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... We incubated sections overnight at 4°C with a primary c-Fos antibody in blocking buffer (1:5000, rabbit anti-c-Fos, Synaptic Systems, 226 003), a primary FosB/ΔFosB antibody (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... and Syt1Ab uptake in the concentration indicated as follows: rabbit antibodies against Syt1 (labelled with Oyster 550; live staining: 1:70, # 105103C3, Synaptic System, Göttingen, Germany), pThr138SNAP25 (WB:1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with shaking in primary antibodies diluted in milk (1:1000 Rabbit-anti-Neuroligin2 (Synaptic Systems 129-202), or 1:200 Mouse-anti-HA-tag (supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were then incubated for 1h at room temperature with primary antibodies in blocking buffer (1:500 Rabbit anti-NL2 (Synaptic Systems, 129-203), or 1:400 Mouse anti-MPM2 (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Nitrocellulose membranes were blocked with 5% milk in TBST buffer and used to detect m6A levels by probing with m6A specific antibodies (Synaptic Systems; 202 003). Images were taken by Amersham Biosciences Imager 600 (GE Healthcare ...
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bioRxiv - Genetics 2019Quote: ... Cells were incubated with primary antibodies (1:500 mouse anti-PSD-95 (ABR, IL, USA) or 1:300 mouse anti-gephyrin (Synaptic Systems, Goettingen, Germany) overnight at 4°C in 1% goat serum/PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 1:1000 for IF) and the anti-MAP2 Polyclonal Guinea Pig Antibody (188 004, 1:1000 for IF) were purchased from Synaptic Systems (Goettingen, Germany). The anti-Tyrosine Hydroxylase Antibody (AB9702 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 % Triton X-100 % and 0.2 % BSA in PBS) containing the primary antibody (polyclonal rabbit anti-TH, 1:1000, Synaptic Systems Cat# 213 104) overnight at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the cryo-sections were incubated for 48 h at 4 °C (while shaking) with a solution containing mouse primary antibody against GluA1 (1:1000; Synaptic Systems, Cat# 182011) in 0.01 M PBS with 10 % NGS and 0.1 % Triton X-100.
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bioRxiv - Neuroscience 2022Quote: ... The washed tissue were then moved into new 1.5 ml centrifuge tubes for primary antibody staining (usually diluted 1:100 – 1:500) with: chicken anti-Homer (160006, Synaptic Systems, Goettngen, Germany), guinea pig anti-Bassoon (#141004 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 HZ in the presence of 1:100 anti VGAT luminal domain polyclonal rabbit antibody conjugated to Alexa Fluor 488 (Synaptic Systems 131 103C2). Following washout ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated for two nights in primary antibody (1:500 Rabbit-anti-GABAA-α1; Synaptic Systems 224 203, RRID:AB_2232180 or Rabbit-anti-vGAT, Synaptic Systems 131 002, RRID:AB_887871) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated with a rat anti-cFos primary antibody (1:1000 dilution in 1% BSA in PBST, Synaptic Systems, 226 017) for 24 hours at room temperature on a shaker ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNA at 6 μg were added to 10 μl Slurry of PierceTM Protein A Agarose beads pre-incubated with 1 μg of an anti-m6A polyclonal antibody (Synaptic Systems, 202-003) by rocking at 4°C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were then incubated in the blocking solution containing the guinea pig anti-c-Fos primary antibody (1:1000; Cat No. 226-308, Synaptic Systems; Gottingen, Germany) overnight at 4°C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... RIP-seq was performed according to the “Refined RIP-seq” protocol for low input material (40) with the following specifications: 2 µg anti-m6A antibody (Synaptic Systems 202 003) was conjugated to magnetic beads and incubated with 6 ug total RNA that was previously fragmented to ∼300 nt fragments with 10 mM ZnCl2 in 10 mM Tris-HCl for 4 minutes at 70°C (5% of fragmented RNA was set aside as input) ...
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bioRxiv - Biochemistry 2023Quote: ... BSA in PBS for 1 hour at RT and incubated overnight at 4 °C with primary anti-m6A antibody (Synaptic systems, 202-003) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used with 4% paraformaldehyde/4% sucrose fixation include Synapsin I/II (anti-guinea pig; Synaptic Systems: 106 004; 1:1000 dilution), PSD-95 (anti-rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... spontaneously active synaptic boutons were labeled by incubating cells with CypHer5E-conjugated antibodies against the luminal domain of vGAT (1:500 from a 1 mg/ml stock; Synaptic Systems; Cat#131,103CpH) for 2 h in their respective conditioned culture media at 37 °C and 5 % CO2 prior to imaging.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at RT with one of the following primary antibodies: guinea pig anti-c-Fos (1:2000, Synaptic Systems, Cat#226004), rabbit anti-CGRP (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... with one or a mix of the following primary antibodies: Rat anti-c-Fos (1:1000, Cat. No. 226 017, Synaptic Systems, RRID: AB_2864765), Rabbit anti-c-Fos (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was incubated overnight at 4°C in primary antibody diluted in blocking solution [rabbit anti-cFos 1:1000 (Synaptic Systems, Cat#20079)] ...
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bioRxiv - Cell Biology 2024Quote: ... 10% of RNA fragments were saved as “Input,” and the remaining fragments were used for m6A immunoprecipitation (IP) using a specific anti-m6A antibody (Synaptic Systems, 202203, Germany). RNA samples from both input and IP were prepared using TRIzol reagent (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... blocking solution for 1 hr at room temperature and incubated overnight at 4 °C with rabbit c-fos antibody (1:5000 dilution, Synaptic Systems, Goettingen, Germany). Following incubation with the primary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... 2015) using 10 μg of purified mRNA from Drosophila S2R+ cells and 5 μg of anti-m6A antibody (Synaptic Systems, Lot# 202003/2-82). Immunoprecipitations were performed in quadruplicates and as a control one immunoprecipitation was performed where UV-crosslinking was ommited ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at room temperature with the first primary antibody (rabbit anti-Syt1, anti-Syt4 or anti Syt7 from Synaptic Systems, Germany; 1:1000), followed by 2h with a first secondary antibody (rabbit Alexa Fluor-488–conjugated ...
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bioRxiv - Neuroscience 2021Quote: ... cultured hippocampal neurons were incubated live for 10 min at 37 °C with a mouse monoclonal antibody raised against the N-terminal domain of GluA2 that also recognizes GluA1 and GluA3 (Synaptic Systems, 182411 Clone 248B7) and diluted to 1:100 in the culture medium ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were rinsed in TBST and cut to be incubated for 1 h at room temperature in 0.5% non-fat dried milk in TBST containing the appropriate primary antibody anti-SP (rabbit polyclonal, Synaptic Systems, 163 002, 1:1000), anti-Beta-actin (mouse monoclonal ...
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bioRxiv - Neuroscience 2022Quote: ... Inhibitory presynaptic sites were detected by immunolabeling against vesicular GABA transporter (vGAT) using rabbit anti-vGAT antibody (1:100, Synaptic Systems, catalog # 131002, RRID:AB_887871). Inhibitory postsynaptic sites were detected by immunolabeling against gephyrin using mouse anti-gephyrin antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... and stained according to the immunohistochemistry protocol above (primary antibodies: anti-ZnT3, rabbit polyclonal, 1:500, Synaptic Systems; anti-puromycin 1:1000, EMD Millipore) and imaged on the Zeiss LSM 880 with Airyscan using a LD LCI Plan-Apochromat 25x/0.8 mm Korr DIC M27 and 1.8x zoom ...
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bioRxiv - Neuroscience 2023Quote: ... 20 μg of protein lysate were loaded and analyzed by SDS-PAGE followed by immunoblotting: CaV2.1 α1 CT antibody (Synaptic Systems, #152 203, rabbit polyclonal) diluted 1:1000 (final concentration 2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated in rabbit anti-c-Fos antibody (1:4000 in 3% NGS + 0.1% Triton; Synaptic Systems, Gottingen, Germany; Lot# 226003/3-45) for 16 h at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated for 1 day at 4 °C in blocking solution with following antibodies: rabbit anti-cFos (226003, Synaptic Systems; Goettingen, GERMANY, 1:1000); goat anti-Wheat Germ Agglutinin (WGA ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices then underwent a double immunostaining for EYFP and cFos using a primary antibody cocktail of rabbit anti-cFos (1:2000, Synaptic Systems, Cat. No. 226 008) and goat anti-GFP (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was blocked with 10% NGS and 0.3% Triton X-100 at RT for 1h then incubated in primary antibodies for AnkG (1:500, Cat#386-006, Synaptic Systems, Gao and Heldt, 2016) and GABAA α2 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... containing KP-Cre/ZsGreen neurons were processed to detect the cholinergic marker vesicular acetylcholine transporter (VAChT) through the following steps: Guinea pig polyclonal VAChT antibody (#139 103; Synaptic Systems; 1:1000; 12h; RT), biotin-conjugated anti-guinea pig IgG (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.3% Tween-20 PBS for 1 h then incubated in rabbit anti-c-Fos antibody (1:500, Synaptic Systems, catalog # 226-003, RRID: AB_2231974) diluted in the same solution for 72 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked with 10% normal donkey serum in 1xPBS-T (0.5% Triton X-100) for 1 h at room temperature and then probed overnight with rabbit anti-VGAT antibody (Synaptic Systems, cat# 131 003, 1:500) in blocking solution at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 750 mM NaCl and 0.5% (vol/vol) Igepal CA-630) and RNase-free water and incubated with 10 μg m6A antibody (Synaptic Systems, cat. no. 202 003, Goettingen, Germany). The mixture was rotated at 4 °C for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... in 0.3% Triton X-100 for 2 hours before being incubated in rabbit anti-c-Fos antibody (1:4000 in 3% NGS + 0.1% Triton; Synaptic Systems, Goettingen, Germany; Lot# 226003/3-45) for 16 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and then cells were fixed and permeabilized and incubated a second time with primary antibodies to label synaptophysin (1:1000) (α-SYP; Synaptic Systems Cat# 101 004; RRID:AB_1210382) and pHluorin (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... DNA-PAINT samples were incubated for 1 hour at room temperature with an anti-bassoon guinea pig antibody (Synaptic Systems cat. 141 004, 1:1000 dilution) as counterstaining and ...
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bioRxiv - Bioengineering 2022Quote: ... Immunocytochemistry was performed after premixing the primary anti-tubulin antibodies (Ab, #T6199 Sigma-Aldrich, #302211 Synaptic Systems, Göttingen, Germany, #302203 Synaptic Systems, and #ab18251 Abcam, Cambridge, UK), using AbberiorStar635P (AS635P ...