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Citations for Synaptic Systems :
551 - 600 of 621 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... to analyze presynaptic excitatory punctae and the guinea pig anti-vesicular GABA transporter (VGAT) antibody (1:200, cytoplasmic domain, Synaptic Systems, RRID:AB_887871) to evaluate presynaptic inhibitory punctae ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was blocked again (4% BSA, 3% donkey serum, 0.1% Triton) for 1 hour before incubation in synaptobrevin-2 primary antibody (Synaptic Systems; 104 211C3) (1:200 in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 48 hours in rabbit anti-c-Fos primary antibody (1:1000 in blocking solution, 226_003, waiting on RRID approval; Synaptic Systems, Goettingen, Germany). Next ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 48h at 4°C while rocking with a primary antibody: guinea pig anti-Vglut2 (1:500, Synaptic Systems #135404), followed by a 4 h incubation with a secondary donkey anti-guinea pig IgG [F(ab’)2fragments] (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... were washed twice in coIP-solution and consecutively incubated for 1 hour at 4°C with an anti-synaptopodin antibody (rabbit anti-Synaptopodin, Synaptic Systems, #163002). Beads were carefully washed twice to remove unbound antibodies and incubated for 1 hour with the precleared supernatant (‘input’ ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% bovine serum albumin dissolved in 1X PBS + 0.03% Triton) for 1 hour at room temperature with gentle shaking and then incubated with anti-IBA1 primary antibody (chicken; Synaptic Systems (234009); 1:1000 ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... total RNA or mRNA was crosslinked to a charged nylon-based membrane using UV light and was incubated with primary rabbit anti-m6A antibody (Synaptic Systems, 202003) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Subsequently membranes were solubilized with CL-91 and processed as described above for APs with a mixture of anti-Nrx1-3 antibodies (anti-Nrx1, #ABN161, Millipore; anti-Nrx1, Synaptic Systems, #175103) and subjected to MS-analysis.
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bioRxiv - Neuroscience 2024Quote: ... sections were placed in primary antibody (cFos, 1:2000, Cell Signaling, #2250; Vglut, 1:500, Invitrogen, #PA5-119621; Vgat, 1:500, Synaptic Systems, #131-004;-Homer ...
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bioRxiv - Neuroscience 2024Quote: ... + 10 mg/ml heparin in PBS (PTwH solution) followed by incubation with primary antibody solution (rabbit anti-Fos, 1:1000; Synaptic Systems 226008) in 5% DMSO + 3% NDS + PTwH at 37°C for 7 d ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then incubated for seven days in a primary antibody solution (rabbit anti-cFos, 1:1000; Synaptic Systems #226-003) diluted in PBS containing 0.2% Tween-20 ...
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bioRxiv - Neuroscience 2020Quote: ... Membrane was then blocked in blocking buffer (5% milk in TBST) for 1 h at room temperature and incubated with N6-mA specific antibody (Synaptic Systems, 1:2000) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the area of manually demarcated complete active zones were also compared with those demarcated using a mixture of active zone-marker antibodies for RIM1/2 (5 µg/ml; Synaptic Systems, Göttingen, Germany), neurexin (5 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies (PV; 1:1000, Swant CS56; 1:100, Sigma-Aldrich; Biotin-WFA; 1:100, Sigma-Aldrich, Gephyrin; 1:200, Synaptic system) were incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies and their dilutions were used: rabbit anti GluN2B (Frontier Institute, 1:100) and rabbit anti synaptoporin (Synaptic Systems, 1:400). The following secondary antibodies and their dilutions were used ...
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bioRxiv - Pathology 2021Quote: ... saturated with PBS 2% BSA for 1 hour at RT and stained with primary antibodies specific for whole VAMP-2 (Synaptic System 104 211). After extensive washing with PBS 0.5% BSA ...
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bioRxiv - Pathology 2021Quote: ... Neutralization was determined by monitoring the cleavage of VAMP-2 with a primary antibody specific for the intact form of the protein (Synaptic System 104 211) and an antibody specific for SNAP-25 (clone SMI81 Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... perineuronal nets were revealed by incubating the neurons with 1:500 mouse anti-TNR antibodies (#217 011; clone 619; Synaptic Systems, Göttingen, Germany) or 1:500 biotinylated Wisteria floribunda agglutinin WFA (#L8258 ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were incubated with 50 μg/mL unlabeled Fab fragments directed against TNR (custom-made from the same TNR antibody used in the rest of the work; Synaptic Systems, Göttingen, Germany), applied together with 1 mg/mL unlabeled anti-mouse nanobody ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were incubated for 5 min with 1:100 polyclonal rabbit antibodies directed against the lumenal domain of Syt1 conjugated to the fluorescent dye Oyster488 (#105 103C2; Synaptic Systems, Göttingen, Germany) in either plain Tyrode (stimulated condition ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 1:500 polyclonal rabbit antibodies directed against the lumenal domain of Syt1 conjugated to the fluorescent dye Oyster488 (#105 103C2; Synaptic Systems, Göttingen, Germany) during the TNR labeling step.
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bioRxiv - Neuroscience 2020Quote: ... surface TNR epitopes were blocked with 10 μg/mL unlabeled Fab fragments directed against TNR (custom-made from the same TNR antibody used in the rest of the work; Synaptic Systems, Göttingen, Germany), applied together with 1 mg/mL unlabeled anti-mouse nanobody (custom-made ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated 48 hrs at 4°C with the following primary antibodies: rabbit anti-cFos (1:1000, Synaptic Systems, #226 003), rabbit anti-TH (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... and Syt1Ab uptake in the concentration indicated as follows: rabbit antibodies against Syt1 (labelled with Oyster 550; live staining: 1:70, # 105103C3, Synaptic System, Göttingen, Germany), pThr138SNAP25 (WB:1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with shaking in primary antibodies diluted in milk (1:1000 Rabbit-anti-Neuroligin2 (Synaptic Systems 129-202), or 1:200 Mouse-anti-HA-tag (supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were then incubated for 1h at room temperature with primary antibodies in blocking buffer (1:500 Rabbit anti-NL2 (Synaptic Systems, 129-203), or 1:400 Mouse anti-MPM2 (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Nitrocellulose membranes were blocked with 5% milk in TBST buffer and used to detect m6A levels by probing with m6A specific antibodies (Synaptic Systems; 202 003). Images were taken by Amersham Biosciences Imager 600 (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... 1:1000 for IF) and the anti-MAP2 Polyclonal Guinea Pig Antibody (188 004, 1:1000 for IF) were purchased from Synaptic Systems (Goettingen, Germany). The anti-Tyrosine Hydroxylase Antibody (AB9702 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 % Triton X-100 % and 0.2 % BSA in PBS) containing the primary antibody (polyclonal rabbit anti-TH, 1:1000, Synaptic Systems Cat# 213 104) overnight at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the cryo-sections were incubated for 48 h at 4 °C (while shaking) with a solution containing mouse primary antibody against GluA1 (1:1000; Synaptic Systems, Cat# 182011) in 0.01 M PBS with 10 % NGS and 0.1 % Triton X-100.
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bioRxiv - Neuroscience 2022Quote: ... The washed tissue were then moved into new 1.5 ml centrifuge tubes for primary antibody staining (usually diluted 1:100 – 1:500) with: chicken anti-Homer (160006, Synaptic Systems, Goettngen, Germany), guinea pig anti-Bassoon (#141004 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 HZ in the presence of 1:100 anti VGAT luminal domain polyclonal rabbit antibody conjugated to Alexa Fluor 488 (Synaptic Systems 131 103C2). Following washout ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated for two nights in primary antibody (1:500 Rabbit-anti-GABAA-α1; Synaptic Systems 224 203, RRID:AB_2232180 or Rabbit-anti-vGAT, Synaptic Systems 131 002, RRID:AB_887871) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated with a rat anti-cFos primary antibody (1:1000 dilution in 1% BSA in PBST, Synaptic Systems, 226 017) for 24 hours at room temperature on a shaker ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNA at 6 μg were added to 10 μl Slurry of PierceTM Protein A Agarose beads pre-incubated with 1 μg of an anti-m6A polyclonal antibody (Synaptic Systems, 202-003) by rocking at 4°C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were then incubated in the blocking solution containing the guinea pig anti-c-Fos primary antibody (1:1000; Cat No. 226-308, Synaptic Systems; Gottingen, Germany) overnight at 4°C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... RIP-seq was performed according to the “Refined RIP-seq” protocol for low input material (40) with the following specifications: 2 µg anti-m6A antibody (Synaptic Systems 202 003) was conjugated to magnetic beads and incubated with 6 ug total RNA that was previously fragmented to ∼300 nt fragments with 10 mM ZnCl2 in 10 mM Tris-HCl for 4 minutes at 70°C (5% of fragmented RNA was set aside as input) ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used with 4% paraformaldehyde/4% sucrose fixation include Synapsin I/II (anti-guinea pig; Synaptic Systems: 106 004; 1:1000 dilution), PSD-95 (anti-rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... spontaneously active synaptic boutons were labeled by incubating cells with CypHer5E-conjugated antibodies against the luminal domain of vGAT (1:500 from a 1 mg/ml stock; Synaptic Systems; Cat#131,103CpH) for 2 h in their respective conditioned culture media at 37 °C and 5 % CO2 prior to imaging.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at RT with one of the following primary antibodies: guinea pig anti-c-Fos (1:2000, Synaptic Systems, Cat#226004), rabbit anti-CGRP (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... with one or a mix of the following primary antibodies: Rat anti-c-Fos (1:1000, Cat. No. 226 017, Synaptic Systems, RRID: AB_2864765), Rabbit anti-c-Fos (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was incubated overnight at 4°C in primary antibody diluted in blocking solution [rabbit anti-cFos 1:1000 (Synaptic Systems, Cat#20079)] ...
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bioRxiv - Cell Biology 2024Quote: ... 10% of RNA fragments were saved as “Input,” and the remaining fragments were used for m6A immunoprecipitation (IP) using a specific anti-m6A antibody (Synaptic Systems, 202203, Germany). RNA samples from both input and IP were prepared using TRIzol reagent (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... blocking solution for 1 hr at room temperature and incubated overnight at 4 °C with rabbit c-fos antibody (1:5000 dilution, Synaptic Systems, Goettingen, Germany). Following incubation with the primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies included PCP4 (Invitrogen, PA5-52209, 1:500, raised in rabbit, and Synaptic Systems, 480 004, 1:500, raised in Guinea pig), GFP (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... Molecular weight regions above 50 kDa were cut from the membrane and incubated overnight at 4°C with primary antibodies against Syt1 (cat# 105 011, Synaptic Systems, Goettingen, Germany) and the region below 50 kDa was incubated with anti-GAPDH (cat#2198 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2015) using 10 μg of purified mRNA from Drosophila S2R+ cells and 5 μg of anti-m6A antibody (Synaptic Systems, Lot# 202003/2-82). Immunoprecipitations were performed in quadruplicates and as a control one immunoprecipitation was performed where UV-crosslinking was ommited ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at room temperature with the first primary antibody (rabbit anti-Syt1, anti-Syt4 or anti Syt7 from Synaptic Systems, Germany; 1:1000), followed by 2h with a first secondary antibody (rabbit Alexa Fluor-488–conjugated ...
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bioRxiv - Neuroscience 2021Quote: ... cultured hippocampal neurons were incubated live for 10 min at 37 °C with a mouse monoclonal antibody raised against the N-terminal domain of GluA2 that also recognizes GluA1 and GluA3 (Synaptic Systems, 182411 Clone 248B7) and diluted to 1:100 in the culture medium ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were rinsed in TBST and cut to be incubated for 1 h at room temperature in 0.5% non-fat dried milk in TBST containing the appropriate primary antibody anti-SP (rabbit polyclonal, Synaptic Systems, 163 002, 1:1000), anti-Beta-actin (mouse monoclonal ...