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Citations for Worthington Biochemical :
251 - 280 of 280 citations for 6 Amino 2 bromopurine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Mouse adult cardiocytes were obtained by digesting adult mouse hearts by retrograde perfusion of a solution of collagenase type 2 (Worthington, Lakewood, NJ) in calcium-free solutions ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/ml of papain (Worthington Biochem., USA), 1 mM EDTA (PanReac AppliChem ...
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bioRxiv - Bioengineering 2021Quote: ... Muscles were minced to create a homogenous cell slurry and then incubated for 2 h at 37C in 0.2% collagenase (Worthington Biochemicals, Lakewood, NJ) in DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... ovarian cortex samples were digested in DMEM containing 25 mM HEPES and 2 mg/mL collagenase type III (Worthington Biochemical Corporation, LS004183) for 2 hours at 37°C with occasional swirling ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... The hippocampal neurons were then dissociated via incubation at 37°C for 30 minutes in 2% papain solution (Worthington; catalog no. LS003127). Following incubation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Systems Biology 2022Quote: ... testes were placed in a 15 ml conical tube containing 2 ml of prewarmed (35 °C) collagenase solution (1 mg/ml type I collagenase (Worthington, cat. # LS004196) and 60 U/ml Turbo Nuclease (Nacalai ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2019Quote: ... An excised muscle was pinned to the bottom of a 3.5 cm Petri dish lined with Silgard and was enzymatically digested using 4mg/ml type-2 collagenase (Worthington Biochemical Corporation, USA) dissolved in HEPES-buffered Tyrode ...
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bioRxiv - Physiology 2020Quote: ... the heart was perfused with the same solutions with 50 μM Ca2+ and collagenase type II (1 mg/mL, CLS-2, Worthington, NJ, USA) for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... and cut into rings (about 1 mm in length) that were subsequently incubated in 2 mg/ml collagenase type II (Worthington Biochemical Corporation) for 2 hr ...
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bioRxiv - Neuroscience 2023Quote: ... The digestion solution is then aspirated off and the tissue is resuspended in a 2 mg/ml trypsin inhibitor ovomucoid solution (Lo Ovo) (Worthington, Cat# LS003083). Cells were then spun in a centrifuge at 300g for 11 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Tissue samples were dissected into small pieces and digested with 1-2 mg/ml (275 U/ml) collagenase type II (Worthington, Columbus, USA) in 30 ml Dulbecco’s minimal essential medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: The primary neuronal cells were isolated from the brain tissues of 1∼2-day-old SD pups using the papain dissociation system (Worthington Biochemical Corporation) as instructed by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... for 5 min at 4°C to remove circulating cells and then digested in RPMI 1640 with 1 mg/mL Type 2 collagenase (Worthington Biochemical LS004177) for 45 min at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
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bioRxiv - Bioengineering 2024Quote: ... the cusps were denuded of their endothelium before being minced and placed into an enzymatic mixture of 2 mg/mL collagenase III (Worthington Biochemical, Lakewood, NJ), 0.1 mg/mL hyaluronidase (Worthington Biochemical ...
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bioRxiv - Microbiology 2024Quote: ... cells were cultured in 24-well plates to ∼95% confluency and then infected in triplicate wells for each time point in Opti-MEM™ medium (Thermo Fischer Scientific, Waltham, MA) supplemented with 1 µg/ml L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK, Worthington Biochemicals, Lakewood, NJ). The starting multiplicity of infection (MOI ...
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Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: ... Once fluids ran clear the perfusion was switched to an isolation buffer which included 2 mg/mL collagenase type II (Worthington Biochemical, catalog: LS004176) for 7 minutes at 2 mL/min ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2019Quote: ... Tissues were cut and minced with scissors and placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) and incubated at 37 °C for 40 min with gentle shaking at 100rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Organoid tissue retained on the filter was then back-flushed with 25 ml of cold HBSS and this tissue was further disaggregated in 2 ml Collagenase II (Celase GMP, Worthington Biochemical Corporation, Lakewood, NJ) also containing 200 μg DNAse1 ...
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bioRxiv - Cell Biology 2021Quote: ... Each tissue was cut and minced with scissors and then placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) for 40 min of incubation at 37°C with gentle shaking at 100 rpm ...
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bioRxiv - Biophysics 2021Quote: ... The excess tissue was removed from the cerebral cortex and the cortices were minced (0.5 mm) and digested in a solution of Hibernate A (HA)–CaCl2 (Brain Bits LLC) + Papain (2 mg/mL; Worthington, Biochemical Corporation, Lakewood, NJ) for 30 minutes at 37°C with gentle agitation every 5 minutes ...
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bioRxiv - Microbiology 2019Quote: ... tissue supernatants were serially diluted 10-fold in virus infection medium supplemented with 1 µg/mL L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ). The plates were rinsed twice with PBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 12-to 16-week old) were euthanized and flexor digitorum brevis (FDB) muscles were dissected and incubated with 2 mg/mL collagenase (Worthington Biochemical Co., NJ, USA) in the HEPES-Krebs solution containing 2 mg/mL bovine serum albumin for 2-3 hours at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The viruses were propagated in MDCK cells in the presence of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ).
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM Na-pyruvate) and dissociated them by adding an equal volume of 2 x Papain stock solution (Worthington Biochemical Corporation, Lakewood, USA) at 37°C for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... These chunks were immersed in 5 ml of pre-warmed enzyme solution (2 mg/mL STEMzyme I, 4 µg/mL DNAse I obtained from Worthington Biochemical #LS004107 and #LS002139) in Hanks’ Balanced Salt Solution (HBSS ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004107, 5x50 mg, $495.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... the digestion was stopped by a solution of trypsin inhibitors (Leibovitz’s L15 medium, 10% 1 mg/ml trypsin inhibitor [Worthington], and 2% 10 mg/ml DNAse) and cells were washed with L15 medium ...
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bioRxiv - Physiology 2019Quote: ... Heart tissue (infarct and surrounding border) was digested in Collagenase II and DNase 1 (600 U/ml Collagenase II; CLS-2 Worthington, 60 U/ml DNAse 1; Ambion, Warrington) in Hank’s Balanced Salt Solution (HBSS ...