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Citations for AAT Bioquest :
1 - 50 of 53 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... cells were loaded at 37°C for 1 hr with 10 μg/ml Fluo-8 AM (AAT Bioquest, Inc.) supplemented with 0.01% Pluronic F-127 (w/v ...
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bioRxiv - Microbiology 2022Quote: ... virus dilutions and number of deaths/group after challenge were used to calculate the LD50 (Quest Graph™ LD50 Calculator, AAT Bioquest, Inc.). The Kaplan-Meier curve was used to show survival rate differences among groups of animals challenged with different doses of SARS-CoV-2 virus ...
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bioRxiv - Immunology 2023Quote: ... virus dilutions and number of deaths/group after challenge were used to calculate the LD50 (Quest Graph™ LD50 Calculator, AAT Bioquest, Inc., S. Francisco, CA, USA). When indicated ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... for 20 min at 37°C with Hoechst 33342 (AAT Bioquest #17535).
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... for 20 min at 37°C with or without Hoechst 33342 (AAT Bioquest #17535).
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bioRxiv - Biochemistry 2021Quote: ... using calculated extinction coefficients (ExPASy, ProtParam software for protein; AAT Bioquest for crRNA).The concentration of VmeCmr complex was estimated using an extinction coefficient of 610,240 M−1 cm−1 ...
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bioRxiv - Neuroscience 2023Quote: The DRACC1 protein was labeled by iFluor 488- or iFluor 568-succinimidyl ester (AAT Bioquest) as previously described (Hosokawa et al. ...
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bioRxiv - Biochemistry 2021Quote: ... the DNA template has a 5’ C and the dNTPs included 0.5 μM of fluorescein-12-dGTP (AAT Bioquest) in addition to 50 μM of dATP ...
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bioRxiv - Neuroscience 2023Quote: iDANoids were incubated for 30 min at 37 °C with 3 µM Calbryte 520 AM calcium indicator (AAT Bioquest) in maturation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: Purified proteins were labeled by iFluor-488/568/633 succinimidyl ester (AAT Bioquest 1023/1049/1030) as previously described 28 ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC-microglia were loaded for 20 min at 37° C with either Cal520 AM or Cal590 AM (5 μM, AAT Bioquest), and the cell permeable ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at 37 °C for 30 minutes in oxygenated aCSF containing 5 μl of Cal520 AM (AAT Bioquest), 1 mM DMSO and 20% pluronic acid ...
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bioRxiv - Neuroscience 2023Quote: ... Assay plates for detection of intracellular calcium were washed twice in phosphate buffered saline (PBS) before incubated (37 °C for at least 30 min) with DMEM containing 2 µM of FluoAM-8 dye (AAT Bioquest) per well ...
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bioRxiv - Bioengineering 2023Quote: ... Tissues were first incubated at 37°C for 90 minutes in Tyrode’s solution containing 5 µM calcium indicator Cal 520 AM (AAT Bioquest, 21130) and 0.025% Pluronic F127 (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Attached cells were loaded with the Ca2+ dye Fluo-8 at room temperature (23 – 25 °C) by adding 4 μM Fluo-8 AM (AAT Bioquest) and 0.02% Pluronic F-127 (SiChem ...
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bioRxiv - Immunology 2023Quote: ... After a wash step the latter was detected with PE-Cy7 conjugated streptavidin protein (AAT bioquest, 16917). The proportion of antibody-bound microbes was evaluated on a bacterial gate based on SSC-A/FSC-A to differentiate debris and microbial aggregates from bacteria ...
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bioRxiv - Biophysics 2022Quote: ... #14175103)] followed by incubation for 1 hour at 37°C and 5% CO2 in 1800 µl of imaging buffer containing 3 mM Cal-520-AM (AAT Bioquest; #21130) Cal-520-AM-loaded cells were removed from the CO2 incubator and equilibrated at room temperature for 5 minutes prior to IP3 stimulation by the addition of 200 µl of 1 mM carbachol (Alfa Aesar ...
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bioRxiv - Physiology 2024Quote: ... and incubated at 37°C under continued static or FSS conditions for 3 hours with 50-1000 nM Texas Red gentamicin conjugate (GTTR; AAT Bioquest, 24300) apically in DF+H medium ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... Cell monolayers were treated with 50 µM BODIPY™ FL C5-Ganglioside GM1 for 20 minutes at 37°C with Hoescht 33342 (AAT Bioquest #17535).
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bioRxiv - Biochemistry 2021Quote: ... DCP2 proteins for fluorescence microscopy studies were labelled with maleimide-reactive XFD488 dye (AAT Bioquest #1878, identical to Alexafluor488) using a similar protocol as for Cy3-labelling of the protein ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Neuroscience 2023Quote: ... protected from light (iFluor488 1:1000 AAT Bioquest #16773, iFluor 555 1:1000 AAT Bioquest, #16831 ...
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bioRxiv - Immunology 2023Quote: The conjugations of peptides to the KLH protein carrier were performed according to the manufacturer instructions of ReadiLink™ KLH Conjugation Kit (AAT Bioquest, Cat.#5502). In brief ...
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bioRxiv - Physiology 2023Quote: ... 1 µM Fura-FF (AAT Bioquest #21028) was used to monitor extra-mitochondrial Ca2+ and 4.8 µM JC-1 (Enzo Life Sciences #52304 ...
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bioRxiv - Neuroscience 2023Quote: ... Oregon Green 488 BAPTA-1 (AAT Bioquest). To do this ...
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bioRxiv - Physiology 2023Quote: ... 1 µM Fura-FF (AAT Bioquest #21028) was used to monitor extra-mitochondrial Ca2+ and 4.8 µM JC-1 (Enzo Life Sciences #52304 ...
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bioRxiv - Microbiology 2023Quote: ... Half of the wells in the plate were stained with eDNA-specific DiTO-1 (1 μM, AAT Bioquest, #17575) for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:250 OxiVisionTM Green peroxide sensor (AAT Bioquest, Cat No ...
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bioRxiv - Neuroscience 2023Quote: ... protected from light (iFluor488 1:1000 AAT Bioquest #16773 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or Cal-520 AM (also at 1:500, AAT Bioquest), along with PowerLoad (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... DiYO-1 (CAS 143413-85-8) was purchased from AAT Bioquest. X-31 influenza virus (A/Aichi/68 ...
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bioRxiv - Biochemistry 2021Quote: ... The lipophilic dye FM 4-64 (1 μg/mL) (AAT Bioquest) was used to stain membranes ...
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bioRxiv - Biophysics 2022Quote: ... DiYO-1 (CAS 143413-85-8) was purchased from AAT Bioquest. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanloamine (TR-DHPE ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:400 DAX-J2™ PON Green (AAT Bioquest, Cat No. 16317) at 37 °C for 60 min ...
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bioRxiv - Neuroscience 2023Quote: ... Phalloidin-Alexa FluorTM 594 (1:1000 stock; AAT Bioquest; Cat# ABD-23158) was added in the secondary incubation step ...
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bioRxiv - Cell Biology 2020Quote: ... a cell permeable fluorogenic caspase probe (1:250, AAT Bioquest, Sunnyvale, CA, USA) at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated in 1:500 MitoROS™ 520 stock solution (AAT Bioquest), 10 μM BioTracker ATP-Red Live Cell Dye (Millipore Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Horseradish peroxidase (HRP) or iFluor 546 (AAT Bioquest, 1:300 dilution, Cat# 16958) was used for the detection of dUTP-biotin ...
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bioRxiv - Neuroscience 2023Quote: ... the culture medium was exchanged to 1 μM Cal630-AM (20721, AAT Bioquest) dissolved in 10% Pluronic F-127 with imaging solution containing 140 mM NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... separate wells of neurons were incubated with 1 μM CytoCalcein Violet 450 (AAT Bioquest) and 1 μM propidium iodide (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The conjugates were further incubated with 1.1:1 Cyanine 5 trans-cyclooctene (AAT Bioquest):Met-NHS for a minute and run through NuPAGE 4-12% Bis-Tris premade polyacrylamide gel (Invitrogen by Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... Cells were loaded by incubation with 1-2 μM Cal-520 AM (AAT Bioquest Inc.) in phenol red-free DMEM/F12 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Samples were cooled down on ice and incubated with 5 μM DiYO-1 (AAT Bioquest) for 5 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... after which 4 μL of 1 mg/mL 7-AAD (AAT Bioquest, Sunnyvale, CA, USA) was added ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 1 μM Calbryte-590 AM Ca2+ dye (AAT Bioquest, cat. 20701) or Calbryte-520 AM (AAT Bioquest ...
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bioRxiv - Developmental Biology 2023Quote: ... and/or fluorescently conjugated phalloidin (1:400; iFluor 405 or iFluor594; AAT Bioquest, Sunnyvale CA). Hoechst 33342 (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... about 1 mg/ml of purified TOM-CC was reacted with Cy3-maleimide (AAT Bioquest, USA) at a molar ratio complex to dye of 1:5 in 20 mM HEPES (pH 7.2) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... transparent 96-well plates were coated with 1 μg/mL BSA-conjugated fentanyl (AAT Bioquest, Pleasanton, CA). Cell culture medium was diluted 100 times and added to the wells to incubate for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the media in each plate was replaced with 40 μL of 1× Hank’s Balanced Salt Solution (HBSS) containing 20 mM M coelenterazine h (AAT Bioquest). The assay plate was incubated at 27 °C in the dark for 4 h ...
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bioRxiv - Bioengineering 2023Quote: Calcium handling analysis was performed by incubating cells for 1 hour at 37 ℃ with 5 μM Cal520-AM (AAT Bioquest). Cells were then returned to conditioned media preserved prior to adding the calcium sensitive dye and allowed to equilibrate for >30 min at 37 ℃ and 5% CO2 ...