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Citations for AAT Bioquest :
1 - 50 of 65 citations for Cyanine2 NHS ester minimal dye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Cy3/405 NHS ester (AAT Bioquest), Alexa Fluor 488 NHS aster (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... or Cy3 NHS ester (AAT Bioquest; 271) were dissolved in DMSO at a concentration of 10 mg/mL and incubated with the corresponding proteins at 1:1 (molar ratio ...
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bioRxiv - Cell Biology 2023Quote: ... or Cy3 NHS ester (AAT Bioquest; 271) were dissolved in DMSO at a concentration of 10 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: Fluro 488 NHS ester (AAT Bioquest; 1810) or Cy3 NHS ester (AAT Bioquest ...
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bioRxiv - Neuroscience 2024Quote: ... and iFluor 405 NHS ester (AAT Bioquest), were dissolved in DMSO at a stock concentration of 5 mM and stored at -20°C ...
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bioRxiv - Biochemistry 2022Quote: ... or iFluor-488/Cy3 NHS ester (AAT Bioquest) were dissolved by DMSO making stock solutions at the concentration of 10 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: The Fluro 488 NHS ester (AAT Bioquest; 1810) or Cy3 NHS ester (AAT Bioquest ...
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bioRxiv - Biochemistry 2024Quote: ... were fluorescently labeled with Atto647N NHS ester (AAT Bioquest, 2856) following manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1 mg of NIR-resonant Raman dyes of SQ-740-NHS (SQ740, SETA Biomedicals) or Tide Quencher™ 7WS succinimidyl ester (T7WS, AAT Bioquest) was added to the incubating solution and held at room temperature overnight ...
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bioRxiv - Neuroscience 2024Quote: JF646-NHS Ester (24 mg, 40 μmol, 1.0 eq., obtained from AAT Bioquest) and HaloTag(O2)amine (13 mg ...
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bioRxiv - Neuroscience 2024Quote: ... by using succinimidyl ester (AAT bioquest)41.
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bioRxiv - Cell Biology 2023Quote: ... HRP-NHS (AAT Bioquest, Ref. 11025), sialidase (neuraminidase from Arthrobacter ureafaciens ...
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bioRxiv - Neuroscience 2020Quote: ... PSD-95 and CaMKII was labeled by iFluor 405 succinimidyl ester or iFluor 488 succinimidyl ester (AAT Bioquest, CA, USA) as previously described 13 ...
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bioRxiv - Cell Biology 2024Quote: ... and JC10 dye (AAT Bioquest) for MMP ...
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bioRxiv - Bioengineering 2024Quote: IL-12 was labeled with indocyanine green (ICG) tetrafluorophenyl (TFP) ester (AAT Bioquest) by solubilizing the dye in dimethyl sulfoxide at 1 mg/mL and adding it to IL-12 at 3 mg/mL in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescent calcium dye (Calbryte 520, AAT Bioquest) was loaded for 40 minutes prior to imaging.
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bioRxiv - Biochemistry 2021Quote: ... HspB8 and variants thereof were labelled with Cyanine-3-monosuccinimidyl ester (AAT bioquest, ABD-141) at equimolar ratio in HspB8 buffer and excess dye was removed by dialysis against HspB8 buffer with 1 mM DTT ...
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bioRxiv - Physiology 2022Quote: Excised hearts were loaded with Rhod-2 AM (rhodamine 2 acetoxymethyl ester; 5μM, AAT Bioquest) in Krebs–Henseleit (KH ...
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bioRxiv - Neuroscience 2023Quote: The DRACC1 protein was labeled by iFluor 488- or iFluor 568-succinimidyl ester (AAT Bioquest) as previously described (Hosokawa et al. ...
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bioRxiv - Cell Biology 2024Quote: ... and the corresponding dye iFluorTM568-streptavidin (AAT Bioquest) were used ...
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bioRxiv - Molecular Biology 2024Quote: ... TCO-dyes used were TCO-Cy3 (AAT Bioquest), TCO-Cy5 (Click Chemistry Tools) ...
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bioRxiv - Neuroscience 2024Quote: ... the calcium-sensitive dye Cal-590 (Aat Bioquest) was dissolved in 4 µl pluronic F-127 ...
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bioRxiv - Neuroscience 2023Quote: Purified proteins were labeled by iFluor-488/568/633 succinimidyl ester (AAT Bioquest 1023/1049/1030) as previously described 28 ...
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bioRxiv - Physiology 2024Quote: ... a potentiometric dye was loaded (62 μg RH237 AAT Bioquest). The epicardial surface was illuminated using high-powered LED light sources (Solis-525C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and/or a potentiometric dye (62 μg RH237; AAT Bioquest). Fluorescence signals were acquired by illuminating the epicardial surface with an LED light with excitation filter (535 ± 25 nm ...
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bioRxiv - Neuroscience 2023Quote: ... A dye solution of 1mM Cal-520 AM (AAT Bioquest), 2.5% Alexa Fluor™ 568 Hydrazide (Thermo Fisher) ...
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bioRxiv - Biochemistry 2021Quote: ... The lipophilic dye FM 4-64 (1 μg/mL) (AAT Bioquest) was used to stain membranes ...
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bioRxiv - Genetics 2024Quote: ... and analyzed using Ca2+ indicator dye Cal-520 AM (Aat Bioquest). The cells were loaded with 1 µM Cal-520 and incubated at 37 °C for 30 min in modified Krebs solution (150 NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... the AM-dye Cal 520 (1 µM, AAT Bioquest, CA, USA) and Alexa 594 (50 µM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3 µL Calbryte™ 520NW dye-loading solution (AAT Bioquest, # 36319) was dispensed into each well ...
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bioRxiv - Neuroscience 2022Quote: ... The neurons were then stained with a JC-10 dye (AAT Bioquest) before having their mitochondrial membrane potential measured by detecting signals in two wavelengths emitted by the dye ...
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bioRxiv - Biophysics 2024Quote: For labelling of LifA with the fluorescent dye Cy3B maleimide (AAT Bioquest), washing and elution during IMAC were performed at pH 8.0 to have LifA in suitable buffer for the subsequent conjugation reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM MitoTracker Green FM and 100 nM MitoTracker Deep Red FM or 200 μM tetramethylrhodamine ethyl ester perchlorate (TMRE, AAT Bioquest). Immediately before imaging ...
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bioRxiv - Neuroscience 2023Quote: Ca2+ imaging was performed by loading calcium dyes Cal 590 (50 μM, AAT Bioquest) together with a structural marker Alexa 488 (100 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... calcium signal was simultaneously measured using Calbryte 590 AM dye (AAT Bioquest Cat #20700) with fluorescence viewed through an mCherry 587/610 excitation and emission filter ...
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bioRxiv - Developmental Biology 2024Quote: ... The calcium dye working solution was prepared by mixing CalbryteTM-520 AM (AAT Bioquest) with 4% FBS-EBM to achieve a final concentration of 5 μM ...
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bioRxiv - Neuroscience 2023Quote: ... agonist-induced increases in fluorescence intensity from an intracellular Ca2+-sensitive dye (Fluo8, AAT Bioquest) were measured in GluK1 and GluK2 expressing cells cultured in 96-well plates using a FlexStation I plate reader (Molecular Devices).
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bioRxiv - Bioengineering 2024Quote: ... Cell suspensions were first stained with viability dye 7-AAD (AAT Bioquest, Sunnyvale, CA, USA), followed by blocking Fc receptors with anti- CD16/32 (clone 93 ...
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bioRxiv - Biophysics 2021Quote: ... His6-FisB ECD G123C was labeled with iFluor555™ maleimide dye (AAT Bioquest, Sunnyvale, CA, USA) by adding a 20x molar excess of dye to the protein and the reaction was incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: [Ca2+]i was monitored using the ratiometric calcium indicator dye CalRed R525/650 AM (AAT Bioquest); when this indicator is excited at 488 nm upon Ca2+ binding ...
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bioRxiv - Cancer Biology 2020Quote: Cell samples were incubated with the calcium indicator dye (4 mM of Cal-520 AM, AAT Bioquest) for 1 hr ...
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bioRxiv - Physiology 2024Quote: ... Hearts were loaded with a calcium indicator dye (50 μg Rhod-2, AM; AAT Bioquest: California US), which was recirculated for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Cardiomyocytes were imaged in Tyrode’s medium containing 5 µM calcium indicator dye Cal 520 AM (21130, AAT Bioquest), and paced at 1 and 2 Hz using two parallel platinum electrodes with an 80V ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with the Ca2+ indicator dye Fluo-4 AM (10 μM, AAT Bioquest, Sunnyvale, CA, USA) for another 30-45 min at 37°C ...
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bioRxiv - Physiology 2023Quote: ... isolated hearts were loaded with a potentiometric dye (RH237: 62 μg rat or guinea pig heart; AAT Bioquest). Fluorescent signals were acquired by illuminating the epicardial surface with a light emitting diode equipped with excitation filter (525 ± 25 nm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated hearts were loaded with a calcium indicator dye (100 μg Rhod-2, AM; AAT Bioquest, Sunnyvale CA) and/or a potentiometric dye (62 μg RH237 ...
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bioRxiv - Biochemistry 2021Quote: ... DCP2 proteins for fluorescence microscopy studies were labelled with maleimide-reactive XFD488 dye (AAT Bioquest #1878, identical to Alexafluor488) using a similar protocol as for Cy3-labelling of the protein ...
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bioRxiv - Cell Biology 2023Quote: ... 40 U/ml RNase inhibitor) were fluorescently labeled with 2 µM of the lipophilic dye DiD Perchlorate (AAT Bioquest) for 30 min on ice ...
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bioRxiv - Physiology 2022Quote: ... we incubated the slices for 50 minutes at RT in the dye-loading solution (6 μM Calbryte 520, AAT Bioquest), 0.03 % Pluronic F-127 (w/v) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells or Matrigel-encased islets from individual animals were loaded with the Ca2+ responsive dye Cal-520 AM (AAT Bioquest), pre-incubated for 1 hr in KRBH G6 ...