Labshake search
Citations for AAT Bioquest :
1 - 50 of 89 citations for 7 Bromo 2H 1 3 benzodioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... and 7-diethylaminocoumarin-3-carboxylic acid was purchased from AAT Bioquest (Sunnyvale, CA). Dibenzocyclooctyne acid was purchased from Click Chemistry Tools (Scottsdale ...
-
bioRxiv - Cell Biology 2024Quote: Caspase-3 activity as an important initiator of apoptosis was assessed by the AmpliteTM Fluorimetric Caspase 3/7 Assay Kit (AAT Bioquest) and performed according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2022Quote: ... or Calbryte™ 630 AM (10 μM, 2h; AAT Bioquest) in HBS ...
-
bioRxiv - Cell Biology 2023Quote: Apoptosis was measured using Cell MeterTM Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in GlomaxTM luminometer at Ex/Em= 490/525 nm.
-
bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
-
bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
-
bioRxiv - Physiology 2020Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
-
bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ No-Wash Live Cell Caspase 3/7 Activity Assay Kits (#20250, AAT Bioquest). Caspase 3/7 activity data were normalized to total number of live cells (determined by CyQUANT kit ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 7-AAD or 7-aminoactinomycine (AAT Bioquest, 17501). Data were acquired on a BD AccuriTM C6 flow cytometer (BD Biosciences ...
-
bioRxiv - Immunology 2020Quote: ... Stained samples were washed and resuspended in 500 μL of 1x PBS + 2% FBS (flow buffer) before 5 μg/mL of 7-AAD (AAT Bioquest) was added ...
-
bioRxiv - Bioengineering 2022Quote: ... after which 4 μL of 1 mg/mL 7-AAD (AAT Bioquest, Sunnyvale, CA, USA) was added ...
-
bioRxiv - Cell Biology 2023Quote: ... and fluorescein-PE [N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine] was obtained from AAT Bioquest Inc.
-
bioRxiv - Biochemistry 2020Quote: ... followed by 0.5 μg/mL 7-AAD (AAT Bioquest). Single-stain and FMO (fluorescence-minus-one ...
-
bioRxiv - Biophysics 2022Quote: ... #14175103)] followed by incubation for 1 hour at 37°C and 5% CO2 in 1800 µl of imaging buffer containing 3 mM Cal-520-AM (AAT Bioquest; #21130) Cal-520-AM-loaded cells were removed from the CO2 incubator and equilibrated at room temperature for 5 minutes prior to IP3 stimulation by the addition of 200 µl of 1 mM carbachol (Alfa Aesar ...
-
bioRxiv - Bioengineering 2023Quote: ... The conjugates were further incubated with 1.1:1 Cyanine 5 trans-cyclooctene (AAT Bioquest):Met-NHS for a minute and run through NuPAGE 4-12% Bis-Tris premade polyacrylamide gel (Invitrogen by Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were cooled down on ice and incubated with 5 μM DiYO-1 (AAT Bioquest) for 5 min at RT ...
-
bioRxiv - Microbiology 2019Quote: ... 32 using the AmpliteTM Fluorimetric sn-Glycerol-3-Phosphate (Gro-3-P) Assay Kit (AAT Bioquest). GAC was released from cell wall by sequential digestion with mutanolysin hydrolase and PlyC amidase ...
-
bioRxiv - Developmental Biology 2022Quote: ... TO-PRO-3 (AAT Bioquest 17572) was used to distinguish the live GFP+ cells and added at 1:1000 concentration (in PBS ...
-
bioRxiv - Bioengineering 2023Quote: Calcium handling analysis was performed by incubating cells for 1 hour at 37 ℃ with 5 μM Cal520-AM (AAT Bioquest). Cells were then returned to conditioned media preserved prior to adding the calcium sensitive dye and allowed to equilibrate for >30 min at 37 ℃ and 5% CO2 ...
-
bioRxiv - Physiology 2023Quote: ... 3 μM Fluo-8H AM (AAT Bioquest, Pleasanton, CA) for 15 min at 37 °C and seeded on 5% 3-aminopropyltriethoxysilane-coated coverslip for 15 min at 37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... and Cal590 (5 μM, AAT Bioquest) were used for the indication of mitochondria ...
-
bioRxiv - Microbiology 2020Quote: ... samples were incubated for 5 min with 1.2 µg ml-1 of the membrane dye MM 4-64 (AAT Bioquest Sunnyvale, CA) and 0.5 µg ml-1 of 4′,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Neuroscience 2019Quote: ... or Cal520 AM (5 μM, AAT Bioquest) in Ringer [(mM) ...
-
bioRxiv - Neuroscience 2023Quote: ... Fluo-8 (AAT Bioquest; final 5 μM), Probenecid (final 1 mM) ...
-
bioRxiv - Neuroscience 2022Quote: ... the cells were loaded with Cal590 (3 μg/ml, AAT Bioquest) for 50 minutes and solutions containing OT with various concentrations (0 ...
-
bioRxiv - Bioengineering 2023Quote: ... tissues were incubated at 37°C and 5% O2 for 90 min in Tyrode’s solution containing 5 μM calcium indicator Cal 520 AM (21130, AAT Bioquest) and 0.025% Pluronic F127 (P2443 ...
-
bioRxiv - Immunology 2023Quote: Recombinant TRAF6(50-350) and other variants were labeled with Cyanine 3 (AAT Bioquest), UBE2N ...
-
bioRxiv - Biochemistry 2021Quote: ... HspB8 and variants thereof were labelled with Cyanine-3-monosuccinimidyl ester (AAT bioquest, ABD-141) at equimolar ratio in HspB8 buffer and excess dye was removed by dialysis against HspB8 buffer with 1 mM DTT ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with CalBryte-630AM (AAT Bioquest 20720, 3 µg/mL in 0.3% DMSO) calcium indicator for 30 minutes in the dark ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were subsequently incubated with Cal520-AM (5 µM; AAT Bioquest #21130) and 6-O-[(4,5-Dimethoxy-2-nitrophenyl)methyl]-2,3-O-(1-methylethylidene)-D-myo-Inositol 1,4,5-tris[bis[(1-oxopropoxy)methyl]phosphate] (ci-IP3/PM ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were treated with Fluo-8 AM (5 µM, AAT Bioquest) for 45 min and then washed twice with PBS ...
-
bioRxiv - Immunology 2023Quote: ... NEMO and TAB1(468-504) were labeled with Cyanine 5 (AAT Bioquest), Ub was labeled with iFluor® 488(AAT Bioquest ...
-
bioRxiv - Neuroscience 2021Quote: ... loading with the calcium indicator CalBryte-630AM (AAT Bioquest 20720, 3 μg/mL in 0.3% DMSO) followed the same protocol.
-
bioRxiv - Bioengineering 2021Quote: ... cells were incubated with 3 μL of 10 mM Fluo-8 AM solution (21082, AAT Bioquest, US), 1.2 μL of 10% (w/v) ...
-
bioRxiv - Bioengineering 2023Quote: ... Texas Red-DHPE (1,2-dihexadecanoly-snglvero-3-phosphoethanolamine-[N-(Texas Red sulfonyl)]) was purchased from AAT Bioquest. PEG-biotin (Biotin-PEG SVA ...
-
bioRxiv - Physiology 2021Quote: ... The cells were subsequently incubated with Cal520-AM (5 µM; AAT Bioquest #21130) and ci-IP3/PM (1 μM ...
-
bioRxiv - Neuroscience 2022Quote: ... Relative intracellular pH was measured using Protonex Red 600 (AAT Bioquest, 5 μM). Mitochondrial membrane potential was measured using MitoSpy Red dye (BioLegend ...
-
bioRxiv - Physiology 2021Quote: ... Ratiometric Ca2+ levels were assessed using Fura Red-AM (5 μM; AAT Bioquest). Detailed methodology is included in the supplement.
-
bioRxiv - Physiology 2022Quote: ... The cells were subsequently incubated with Cal520-AM (5 µM; AAT Bioquest #21130) and ci-IP3/PM (1 μM ...
-
bioRxiv - Molecular Biology 2023Quote: ... some of which were further treated with 5 μM Calbryte 520AM (AAT Bioquest) for 30 min ...
-
bioRxiv - Neuroscience 2024Quote: ... iMG were loaded by incubation with Cal-590 AM (5 μM, AAT Bioquest) plus 0.1% Pluronic F-127 for 20 min at 37° C in microglia culture medium and then incubated for further 30 min at room temperature (RT ...
-
bioRxiv - Neuroscience 2023Quote: iDANoids were incubated for 30 min at 37 °C with 3 µM Calbryte 520 AM calcium indicator (AAT Bioquest) in maturation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated with 5 μmol/L Cal-590™ AM (AAT Bioquest, Sunnyvale, CA) with 0.02% Pluronic F127 for 90 min at 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... segregated cardiac μT was stained with 5 μM of Cal-520 AM (AAT Bioquest, 21130), a calcium indicator dye ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells loaded with a cytosolic Ca2+ indicator (5 μM Cal Red R525/650, AAT Bioquest) were imaged by a confocal microscope (SP8 ...
-
bioRxiv - Physiology 2023Quote: ... FDB fibers were loaded with 5 µM fura-FF AM (AAT Bioquest, Sunnyvale, CA, USA), a low-affinity ratiometric Ca2+ dye ...
-
bioRxiv - Genomics 2024Quote: Fluorescent probes were generated using the Nick translation DNA labeling system 2.0 (Enzo) with XFD 488-dUTP or Cyanine-3-dUTP (AAT Bioquest). Unincorporated nucleotides were removed using QIAquick PCR Purification Kit (Qiagen) ...
-
bioRxiv - Immunology 2022Quote: ... CD4+ T cells were loaded with 5 μM Cal Red™ R525/650 AM (AAT Bioquest) at room temperature for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: ... Spinal cord slices were incubated with Calbryte 520 AM (5 μM in RaCSF, 20650, AAT Bioquest) for 30-300 min at RT ...
-
bioRxiv - Bioengineering 2023Quote: ... HLOs on the 36PillarPlate were treated with 5 nM cholyl-lysyl-fluorescein (AAT Bioquest Inc., CA, USA) in a 384-well plate ...