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Citations for AAT Bioquest :
1 - 50 of 128 citations for 7 7a Dihydro 2 2 4 6 6 pentamethyl 1 3 benzodioxol 5 6H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Immunology 2020Quote: ... Stained samples were washed and resuspended in 500 μL of 1x PBS + 2% FBS (flow buffer) before 5 μg/mL of 7-AAD (AAT Bioquest) was added ...
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bioRxiv - Bioengineering 2022Quote: ... after which 4 μL of 1 mg/mL 7-AAD (AAT Bioquest, Sunnyvale, CA, USA) was added ...
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bioRxiv - Bioengineering 2022Quote: ... and 7-diethylaminocoumarin-3-carboxylic acid was purchased from AAT Bioquest (Sunnyvale, CA). Dibenzocyclooctyne acid was purchased from Click Chemistry Tools (Scottsdale ...
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bioRxiv - Cell Biology 2024Quote: Caspase-3 activity as an important initiator of apoptosis was assessed by the AmpliteTM Fluorimetric Caspase 3/7 Assay Kit (AAT Bioquest) and performed according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2022Quote: Excised hearts were loaded with Rhod-2 AM (rhodamine 2 acetoxymethyl ester; 5μM, AAT Bioquest) in Krebs–Henseleit (KH ...
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bioRxiv - Neuroscience 2023Quote: ... and 2% pluronic acid (AAT Bioquest) in ASW was injected into one of the optic lobes using a glass micropipette needle (Harvard Apparatus Cat ...
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bioRxiv - Cell Biology 2023Quote: Apoptosis was measured using Cell MeterTM Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in GlomaxTM luminometer at Ex/Em= 490/525 nm.
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bioRxiv - Biophysics 2020Quote: ... culture medium were discarded and cells were incubated with 50 μl per well of 4 μM Rhod-2/AM (AAT Bioquest) in TARODES’s buffer (10 mM HEPES ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... anti-LFA-2 iFlour568 (AAT Bioquest, # 100210B0), and SiR-Actin (Cytoskeleton ...
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bioRxiv - Cancer Biology 2024Quote: ... and Fura-2-AM (AAT Bioquest, 21023), were individually dissolved in DMSO and frozen in single use aliquots to prevent freeze thaw ...
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bioRxiv - Biophysics 2019Quote: ... Cells were loaded by incubation with 1-2 μM Cal-520 AM (AAT Bioquest Inc.) in phenol red-free DMEM/F12 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
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bioRxiv - Physiology 2020Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
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bioRxiv - Physiology 2022Quote: To analyze voltage changes in the SAN preparation we loaded it by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator Di-4-ANEPPS (2 μmol/L; AAT Bioquest, Sunnyvale, California). This immersion was performed at room temperature (20-22°C ...
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ No-Wash Live Cell Caspase 3/7 Activity Assay Kits (#20250, AAT Bioquest). Caspase 3/7 activity data were normalized to total number of live cells (determined by CyQUANT kit ...
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bioRxiv - Cell Biology 2019Quote: ... stained with 2 μM Fluo-8 AM (AAT Bioquest) for 30 min ...
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bioRxiv - Physiology 2022Quote: ... we incubated the slices for 50 minutes at RT in the dye-loading solution (6 μM Calbryte 520, AAT Bioquest), 0.03 % Pluronic F-127 (w/v) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 7-AAD or 7-aminoactinomycine (AAT Bioquest, 17501). Data were acquired on a BD AccuriTM C6 flow cytometer (BD Biosciences ...
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bioRxiv - Synthetic Biology 2021Quote: Rhod-2 dextran conjugate (~11,000 Da) was from AAT Bioquest. 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) ...
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bioRxiv - Neuroscience 2019Quote: ... we included 0.2 mM Rhod-2 (AAT Bioquest Cat# 21068) in the patch pipette solution ...
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bioRxiv - Physiology 2022Quote: ... myocytes were loaded with Rhod 2-AM (5μM, AAT Bioquest) for 25 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... samples were incubated for 5 min with 1.2 µg ml-1 of the membrane dye MM 4-64 (AAT Bioquest Sunnyvale, CA) and 0.5 µg ml-1 of 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2023Quote: ... was created by dissolving 2 µL stock solution made according to package insert per 1 mL of Assay Buffer (AAT Bioquest Screen Quest Fluorimetric Fatty Acid Uptake Assay Kit #36385) ...
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bioRxiv - Neuroscience 2021Quote: ... Rhod-2 tripotassium salt was purchased from AAT Bioquest (Sunnyvale, CA, USA).
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bioRxiv - Pathology 2022Quote: ... These cells were loaded with Rhod-2 AM (AAT Bioquest, Inc, Catalog #21062) at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg/µl of hIgG (and 15 ng/µl iFluor647-amine (AAT Bioquest), for fluorescent beads ...
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bioRxiv - Cell Biology 2023Quote: ... and fluorescein-PE [N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine] was obtained from AAT Bioquest Inc.
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bioRxiv - Cell Biology 2020Quote: ... Keratinocytes were loaded through the incubation of 2 μM Cal-520 AM (AAT Bioquest Inc.) with 0.04% Pluronic F-127 (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... followed by 0.5 μg/mL 7-AAD (AAT Bioquest). Single-stain and FMO (fluorescence-minus-one ...
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bioRxiv - Biochemistry 2021Quote: ... The lipophilic dye FM 4-64 (1 μg/mL) (AAT Bioquest) was used to stain membranes ...
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bioRxiv - Neuroscience 2022Quote: ... primary microglia of indicated groups were cultured in heat-inactivated DMEM medium containing 5 μM Fluo-4 AM (AAT Bioquest), 0.4 % F-127 (Beyotime ...
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bioRxiv - Physiology 2024Quote: ... Hearts were loaded with a calcium indicator dye (50 μg Rhod-2, AM; AAT Bioquest: California US), which was recirculated for 10 minutes ...
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bioRxiv - Biophysics 2022Quote: ... #14175103)] followed by incubation for 1 hour at 37°C and 5% CO2 in 1800 µl of imaging buffer containing 3 mM Cal-520-AM (AAT Bioquest; #21130) Cal-520-AM-loaded cells were removed from the CO2 incubator and equilibrated at room temperature for 5 minutes prior to IP3 stimulation by the addition of 200 µl of 1 mM carbachol (Alfa Aesar ...
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bioRxiv - Neuroscience 2021Quote: ... were further incubated at the last hour with 2 μM of Cal-590TM AM (AAT Bioquest, CA, USA) in a humidified incubator at 37°C under 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated hearts were loaded with a calcium indicator dye (100 μg Rhod-2, AM; AAT Bioquest, Sunnyvale CA) and/or a potentiometric dye (62 μg RH237 ...
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bioRxiv - Neuroscience 2021Quote: Neurons were incubated in a calcium indicator dye solution consisting of 5 μM Rhod-4 AM (AAT Bioquest #21122; Sunnyvale, CA, USA), 1X PowerLoad (Thermo Fisher Scientific #P10020) ...
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bioRxiv - Cell Biology 2023Quote: ... 40 U/ml RNase inhibitor) were fluorescently labeled with 2 µM of the lipophilic dye DiD Perchlorate (AAT Bioquest) for 30 min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... The conjugates were further incubated with 1.1:1 Cyanine 5 trans-cyclooctene (AAT Bioquest):Met-NHS for a minute and run through NuPAGE 4-12% Bis-Tris premade polyacrylamide gel (Invitrogen by Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Assay plates for detection of intracellular calcium were washed twice in phosphate buffered saline (PBS) before incubated (37 °C for at least 30 min) with DMEM containing 2 µM of FluoAM-8 dye (AAT Bioquest) per well ...
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bioRxiv - Cell Biology 2023Quote: To image cytoplasmic Ca2+ cells were loaded for 30 min at room temperature with either 2 µM Calbryte 590 AM (AAT Bioquest), Fluo4-AM or Fura 2-AM (Molecular Probes) ...
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bioRxiv - Neuroscience 2023Quote: ... the intracellular solution in the second patch contained a Ca2+ indicator at the concentration of 2 mM which was either Cal-520FF (AAT Bioquest, Sunnyvale ...
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bioRxiv - Microbiology 2020Quote: ... Samples were cooled down on ice and incubated with 5 μM DiYO-1 (AAT Bioquest) for 5 min at RT ...
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bioRxiv - Microbiology 2019Quote: ... 32 using the AmpliteTM Fluorimetric sn-Glycerol-3-Phosphate (Gro-3-P) Assay Kit (AAT Bioquest). GAC was released from cell wall by sequential digestion with mutanolysin hydrolase and PlyC amidase ...
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bioRxiv - Developmental Biology 2022Quote: ... TO-PRO-3 (AAT Bioquest 17572) was used to distinguish the live GFP+ cells and added at 1:1000 concentration (in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The normalized intensity was recorded and fitted to equation 2 for determination of the IC50 value (AAT Bioquest, equation adapted in Origin Pro) for the experiment.(48–52 ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal neurons were first incubated in regular culture medium with 2 µM fluorescent calcium indicator Cal-590 AM (#20510, AAT Bioquest, CA, USA) for 60 minutes at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Fluo8-AM (4 μM, AAT Bioquest) was loaded in physiological buffer with sulfinpyrazone ...
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bioRxiv - Bioengineering 2023Quote: Calcium handling analysis was performed by incubating cells for 1 hour at 37 ℃ with 5 μM Cal520-AM (AAT Bioquest). Cells were then returned to conditioned media preserved prior to adding the calcium sensitive dye and allowed to equilibrate for >30 min at 37 ℃ and 5% CO2 ...
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bioRxiv - Physiology 2023Quote: ... 3 μM Fluo-8H AM (AAT Bioquest, Pleasanton, CA) for 15 min at 37 °C and seeded on 5% 3-aminopropyltriethoxysilane-coated coverslip for 15 min at 37 °C ...