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Citations for AAT Bioquest :
201 - 250 of 449 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The calcium indicator Cal-520 (AAT Bioquest, USA) was introduced into the culture medium at a final concentration of 5 μM and perfused for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Assay plates for detection of intracellular calcium were washed twice in phosphate buffered saline (PBS) before incubated (37 °C for at least 30 min) with DMEM containing 2 µM of FluoAM-8 dye (AAT Bioquest) per well ...
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bioRxiv - Neuroscience 2023Quote: ... agonist-induced increases in fluorescence intensity from an intracellular Ca2+-sensitive dye (Fluo8, AAT Bioquest) were measured in GluK1 and GluK2 expressing cells cultured in 96-well plates using a FlexStation I plate reader (Molecular Devices).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... New Zealand White rabbits were immunized subcutaneously with 500 μg of KLH-conjugated fentanyl (AAT Bioquest, Pleasanton, CA) emulsified with complete Freund’s adjuvant (CFA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The activity of various ligands for inducing intracellular calcium mobilization [Ca2+]i was measured using Calbryte 520 AM (AAT Bioquest, USA) using the stably transfected cell lines described above ...
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bioRxiv - Physiology 2023Quote: ... FDB fibers were loaded with 5 µM fura-FF AM (AAT Bioquest, Sunnyvale, CA, USA), a low-affinity ratiometric Ca2+ dye ...
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bioRxiv - Neuroscience 2023Quote: The intracellular Ca2+ levels were measured using Cal-520 (21130, AAT Bioquest, Inc., Sunnyvale, USA) as previously described [15] ...
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bioRxiv - Physiology 2023Quote: ... 1 µM Fura-FF (AAT Bioquest #21028) was used to monitor extra-mitochondrial Ca2+ and 4.8 µM JC-1 (Enzo Life Sciences #52304 ...
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bioRxiv - Immunology 2023Quote: ... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
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bioRxiv - Physiology 2023Quote: ... We used the total antioxidant capacity (TAC) assay kit (Cell Meter™ Colorimetric Antioxidant Activity Assay Kit, # 15900) according to the manufacturer’s protocol (AAT Bioquest). The antioxidant capacity of the sample was compared with that of Trolox ...
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bioRxiv - Neuroscience 2023Quote: ... and purified TfR1 antibody (OKT-9, AAT Bioquest, 10713000) at a 1:200 dilution were added to wells to reach a final concentration of 1 µg/mL each ...
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bioRxiv - Cell Biology 2023Quote: ... Cal-590-AM and Cal520-AM were from AAT Bioquest. BCECF was from Life technologies ...
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bioRxiv - Immunology 2023Quote: ... CytoTell blue or CytoTell green (both form AAT BioQuest). The Ramos B cells were then resuspended in a mixture 1:1 of both tracers and incubated in the dark for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: iDANoids were incubated for 30 min at 37 °C with 3 µM Calbryte 520 AM calcium indicator (AAT Bioquest) in maturation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... protected from light (iFluor488 1:1000 AAT Bioquest #16773 ...
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bioRxiv - Neuroscience 2023Quote: ... protected from light (iFluor488 1:1000 AAT Bioquest #16773, iFluor 555 1:1000 AAT Bioquest, #16831 ...
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bioRxiv - Bioengineering 2023Quote: ... Tissues were first incubated at 37°C for 90 minutes in Tyrode’s solution containing 5 µM calcium indicator Cal 520 AM (AAT Bioquest, 21130) and 0.025% Pluronic F127 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Coelentrazine-H (AAT Bioquest). Stock solutions of compounds were prepared according to manufacturers’ recommendations ...
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bioRxiv - Cell Biology 2023Quote: To image cytoplasmic Ca2+ cells were loaded for 30 min at room temperature with either 2 µM Calbryte 590 AM (AAT Bioquest), Fluo4-AM or Fura 2-AM (Molecular Probes) ...
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bioRxiv - Cancer Biology 2023Quote: ... was derivatized with streptavidin (AAT Bioquest, Cat.#16885) molecules using carbodiimide chemistry via the carboxylic groups of the sugar residues.[46] Coating of the microfluidic chips with a streptavidin derivatized ...
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bioRxiv - Cancer Biology 2023Quote: Quantification of lipids droplets accumulated in PDAC or CAFs cells (5000 cells per well in a 96-well plate) was conducted using the Lipid Droplets Fluorescence Assay Kit (Cayman #No. 500001 or AAT Bioquest #22730) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: A standard curve was generated using the Four Parameter Logistic (4PL) Curve Calculator (AAT bioquest). The 4PL curve was used to calculate the amount of HBs in the supernatants.
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bioRxiv - Neuroscience 2023Quote: ... the intracellular solution in the second patch contained a Ca2+ indicator at the concentration of 2 mM which was either Cal-520FF (AAT Bioquest, Sunnyvale ...
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bioRxiv - Bioengineering 2023Quote: Calcium handling analysis was performed by incubating cells for 1 hour at 37 ℃ with 5 μM Cal520-AM (AAT Bioquest). Cells were then returned to conditioned media preserved prior to adding the calcium sensitive dye and allowed to equilibrate for >30 min at 37 ℃ and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Phalloidin-Alexa FluorTM 594 (1:1000 stock; AAT Bioquest; Cat# ABD-23158) was added in the secondary incubation step ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and/or a potentiometric dye (62 μg RH237; AAT Bioquest). Fluorescence signals were acquired by illuminating the epicardial surface with an LED light with excitation filter (535 ± 25 nm ...
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bioRxiv - Molecular Biology 2023Quote: Dispersed islet cells were laded with 4 μm Calbryte 520 AM (AAT Bioquest 20650) in loading solution (2 mM Glucose in KRBH buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... transparent 96-well plates were coated with 1 μg/mL BSA-conjugated fentanyl (AAT Bioquest, Pleasanton, CA). Cell culture medium was diluted 100 times and added to the wells to incubate for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated hearts were loaded with a calcium indicator dye (100 μg Rhod-2, AM; AAT Bioquest, Sunnyvale CA) and/or a potentiometric dye (62 μg RH237 ...
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bioRxiv - Neuroscience 2023Quote: ... we injected a crystal of a calcium indicator (Cal-520-Dextran Conjugate MW 10’000, AAT Bioquest | Biomol GmbH, Hamburg, Germany) into the medial calixes of both mushroom bodies for a retrograde backfill of AL projection neurons ...
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bioRxiv - Bioengineering 2023Quote: ... CytoTraceTM Red CMTPX (22015) was purchased from AAT Bioquest (CA, USA). Minimum essential medium eagle (MEM ...
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bioRxiv - Neuroscience 2023Quote: ... transfected cells were washed two times in phosphate bovine serum (PBS) buffer and loaded with a FluoAM-8 dye (AAT Bioquest) solution in plain DMEM (r.t.) ...
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bioRxiv - Neuroscience 2023Quote: ... the culture medium was exchanged to 1 μM Cal630-AM (20721, AAT Bioquest) dissolved in 10% Pluronic F-127 with imaging solution containing 140 mM NaCl ...
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bioRxiv - Pathology 2023Quote: ... cAMP was measured by using a cAMP enzyme immunoassay kit (AAT Bioquest, Inc.) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... white opaque 96-well plates were coated with 0.2 μg/mL BSA-conjugated fentanyl (AAT Bioquest, Pleasanton, CA). The NanoLuc-fused antibodies were first incubated with the test samples containing fentanyl or its analogs for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: Intracellular calcium labeling was prepared using Calbryte-590 AM (AAT Bioquest) following manufacturer-provided protocol ...
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bioRxiv - Cell Biology 2023Quote: Apoptosis was measured using Cell MeterTM Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in GlomaxTM luminometer at Ex/Em= 490/525 nm.
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bioRxiv - Physiology 2023Quote: ... 3 μM Fluo-8H AM (AAT Bioquest, Pleasanton, CA) for 15 min at 37 °C and seeded on 5% 3-aminopropyltriethoxysilane-coated coverslip for 15 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... After 10min centrifugation at 10000xg free glucose was measured using the Amplite Fluorimetric Glucose Quantification Kit (AAT Bioquest, Inc., United States) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and 2% pluronic acid (AAT Bioquest) in ASW was injected into one of the optic lobes using a glass micropipette needle (Harvard Apparatus Cat ...
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bioRxiv - Neuroscience 2023Quote: ... A dye solution of 1mM Cal-520 AM (AAT Bioquest), 2.5% Alexa Fluor™ 568 Hydrazide (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: The DRACC1 protein was labeled by iFluor 488- or iFluor 568-succinimidyl ester (AAT Bioquest) as previously described (Hosokawa et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Fluo-8 (AAT Bioquest; final 5 μM), Probenecid (final 1 mM) ...
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bioRxiv - Microbiology 2023Quote: ... Half of the wells in the plate were stained with eDNA-specific DiTO-1 (1 μM, AAT Bioquest, #17575) for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... The conjugates were further incubated with 1.1:1 Cyanine 5 trans-cyclooctene (AAT Bioquest):Met-NHS for a minute and run through NuPAGE 4-12% Bis-Tris premade polyacrylamide gel (Invitrogen by Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 µM Cal-520 (AAT Bioquest). Following a 1 h incubation (37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... HLOs on the 36PillarPlate were treated with 5 nM cholyl-lysyl-fluorescein (AAT Bioquest Inc., CA, USA) in a 384-well plate ...
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bioRxiv - Bioengineering 2023Quote: ... tissues were incubated at 37°C and 5% O2 for 90 min in Tyrode’s solution containing 5 μM calcium indicator Cal 520 AM (21130, AAT Bioquest) and 0.025% Pluronic F127 (P2443 ...
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bioRxiv - Bioengineering 2023Quote: ... Calcein Red AM (AAT Bioquest) was added to the cell solution at a 1:100 volume ratio to stain live cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:400 DAX-J2™ PON Green (AAT Bioquest, Cat No. 16317) at 37 °C for 60 min ...