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Citations for AAT Bioquest :
51 - 100 of 117 citations for 8 4 ethoxy 3 methoxyphenyl 1 5 diazabicyclo 3.2.1 octane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Dispersed islet cells were laded with 4 μm Calbryte 520 AM (AAT Bioquest 20650) in loading solution (2 mM Glucose in KRBH buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Texas Red-DHPE (1,2-dihexadecanoly-snglvero-3-phosphoethanolamine-[N-(Texas Red sulfonyl)]) was purchased from AAT Bioquest. PEG-biotin (Biotin-PEG SVA ...
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bioRxiv - Cell Biology 2023Quote: Apoptosis was measured using Cell MeterTM Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in GlomaxTM luminometer at Ex/Em= 490/525 nm.
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bioRxiv - Physiology 2021Quote: ... The cells were subsequently incubated with Cal520-AM (5 µM; AAT Bioquest #21130) and ci-IP3/PM (1 μM ...
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bioRxiv - Neuroscience 2022Quote: ... Relative intracellular pH was measured using Protonex Red 600 (AAT Bioquest, 5 μM). Mitochondrial membrane potential was measured using MitoSpy Red dye (BioLegend ...
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bioRxiv - Physiology 2021Quote: ... Ratiometric Ca2+ levels were assessed using Fura Red-AM (5 μM; AAT Bioquest). Detailed methodology is included in the supplement.
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bioRxiv - Physiology 2022Quote: ... The cells were subsequently incubated with Cal520-AM (5 µM; AAT Bioquest #21130) and ci-IP3/PM (1 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... some of which were further treated with 5 μM Calbryte 520AM (AAT Bioquest) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... iMG were loaded by incubation with Cal-590 AM (5 μM, AAT Bioquest) plus 0.1% Pluronic F-127 for 20 min at 37° C in microglia culture medium and then incubated for further 30 min at room temperature (RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Isolated single FDB cells were incubated with 4 µM Cal520-AM (AAT Bioquest, CA, USA) or fura-2-AM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... we loaded cytotoxic T cells for 30 minutes with 4 μM Cal-520 (AAT Bioquest), and transferred 2 x 104 cells per well into flat-bottom 96 well plates coated with a CD3 antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... T-tubules from intact hearts were stained with MM 4-64 (2.5 μM, AAT BioQuest) in Ca2+ free Tyrode solution via Langendorff perfusion at room temperature for 30 min as described previously (53 ...
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bioRxiv - Immunology 2024Quote: ... The standard curve was created with an online 4-PL curve calculator from AAT Bioquest (https://www.aatbio.com/tools/four-parameter-logistic-4pl-curve-regression-online-calculator) ...
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
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bioRxiv - Physiology 2020Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
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bioRxiv - Neuroscience 2023Quote: iDANoids were incubated for 30 min at 37 °C with 3 µM Calbryte 520 AM calcium indicator (AAT Bioquest) in maturation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with 5 μmol/L Cal-590™ AM (AAT Bioquest, Sunnyvale, CA) with 0.02% Pluronic F127 for 90 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... segregated cardiac μT was stained with 5 μM of Cal-520 AM (AAT Bioquest, 21130), a calcium indicator dye ...
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bioRxiv - Cell Biology 2022Quote: ... Cells loaded with a cytosolic Ca2+ indicator (5 μM Cal Red R525/650, AAT Bioquest) were imaged by a confocal microscope (SP8 ...
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bioRxiv - Physiology 2023Quote: ... FDB fibers were loaded with 5 µM fura-FF AM (AAT Bioquest, Sunnyvale, CA, USA), a low-affinity ratiometric Ca2+ dye ...
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bioRxiv - Cancer Biology 2020Quote: Cell samples were incubated with the calcium indicator dye (4 mM of Cal-520 AM, AAT Bioquest) for 1 hr ...
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ No-Wash Live Cell Caspase 3/7 Activity Assay Kits (#20250, AAT Bioquest). Caspase 3/7 activity data were normalized to total number of live cells (determined by CyQUANT kit ...
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bioRxiv - Genomics 2024Quote: Fluorescent probes were generated using the Nick translation DNA labeling system 2.0 (Enzo) with XFD 488-dUTP or Cyanine-3-dUTP (AAT Bioquest). Unincorporated nucleotides were removed using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were loaded with 5 μM Cal Red™ R525/650 AM (AAT Bioquest) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Spinal cord slices were incubated with Calbryte 520 AM (5 μM in RaCSF, 20650, AAT Bioquest) for 30-300 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with the Ca2+ indicator dye Fluo-4 AM (10 μM, AAT Bioquest, Sunnyvale, CA, USA) for another 30-45 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... HLOs on the 36PillarPlate were treated with 5 nM cholyl-lysyl-fluorescein (AAT Bioquest Inc., CA, USA) in a 384-well plate ...
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bioRxiv - Physiology 2024Quote: ... and incubated at 37°C under continued static or FSS conditions for 3 hours with 50-1000 nM Texas Red gentamicin conjugate (GTTR; AAT Bioquest, 24300) apically in DF+H medium ...
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bioRxiv - Bioengineering 2020Quote: ... Cardiomyocytes were imaged in Tyrode’s medium containing 5 µM calcium indicator dye Cal 520 AM (21130, AAT Bioquest), and paced at 1 and 2 Hz using two parallel platinum electrodes with an 80V ...
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bioRxiv - Biophysics 2020Quote: ... culture medium were discarded and cells were incubated with 50 μl per well of 4 μM Rhod-2/AM (AAT Bioquest) in TARODES’s buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... T50 was calculated by fitting a 4-parameter logistic equation to the dataset using the calculator at (AAT BioQuest Inc, 2022), see example in Figure S4 ...
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bioRxiv - Biochemistry 2021Quote: ... the DNA template has a 5’ C and the dNTPs included 0.5 μM of fluorescein-12-dGTP (AAT Bioquest) in addition to 50 μM of dATP ...
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bioRxiv - Neuroscience 2021Quote: ... we loaded the ORNs with a calcium sensitive dextran coupled dye (Cal 520 dextran conjugate, 10 kDa, AAT Bioquest; 3 mM in Frog Ringer) via bulk electroporation (Weiss et al. ...
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bioRxiv - Physiology 2022Quote: To analyze voltage changes in the SAN preparation we loaded it by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator Di-4-ANEPPS (2 μmol/L; AAT Bioquest, Sunnyvale, California). This immersion was performed at room temperature (20-22°C ...
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bioRxiv - Immunology 2024Quote: ... then fit to a sigmoidal 4 parameter logistic (4PL) model by utilizing the Four Parameter Logistic (4PL) Curve Calculator (AAT Bioquest Inc.). The sigmoidal 4PL equations were then used to calculate the concentration of individual samples ...
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bioRxiv - Bioengineering 2024Quote: ... HLOs formed in 50 µL Matrigel domes were treated with 5 µM cholyl-lysyl-fluorescein (AAT Bioquest Inc., CA, USA) in complete maturation medium ...
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bioRxiv - Microbiology 2020Quote: The cell length and width were calculated by first staining the cell with the membrane dye MM 4-64 (AAT Bioquest Sunnyvale, CA) to visualize the cell contour ...
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bioRxiv - Biophysics 2019Quote: ... we measured conduction velocity in well-connected cardiac cultures and co-cultures utilizing the dye Rhod-4-AM (10 µM: AAT Bioquest, Sunnyvale, CA). Rhod-4 AM diluted in Tyrode’s solution containing the following in mM ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC-microglia were loaded for 20 min at 37° C with either Cal520 AM or Cal590 AM (5 μM, AAT Bioquest), and the cell permeable ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at 37 °C for 30 minutes in oxygenated aCSF containing 5 μl of Cal520 AM (AAT Bioquest), 1 mM DMSO and 20% pluronic acid ...
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bioRxiv - Biophysics 2021Quote: ... and 262 mM Sucrose with a final concentration of 25 uM Fluo-5N (Stock concentration 5 mM in DMSO, AAT Bioquest). Next ...
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bioRxiv - Immunology 2020Quote: ... Stained samples were washed and resuspended in 500 μL of 1x PBS + 2% FBS (flow buffer) before 5 μg/mL of 7-AAD (AAT Bioquest) was added ...
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bioRxiv - Bioengineering 2023Quote: ... Tissues were first incubated at 37°C for 90 minutes in Tyrode’s solution containing 5 µM calcium indicator Cal 520 AM (AAT Bioquest, 21130) and 0.025% Pluronic F127 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... protected from light (iFluor488 1:1000 AAT Bioquest #16773, iFluor 555 1:1000 AAT Bioquest, #16831 ...
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bioRxiv - Neuroscience 2021Quote: ... we placed dried dye crystals of fluorophore-coupled dextrans (Alexa Fluor dextran 488, 594, Cascade Blue dextran, Thermo Fisher or Cal520 dextran conjugate, AAT Bioquest, 10kDa, 3 mM in Frog Ringer) in the nostrils and applied six electric square pulses using two platinum electrodes (15 V ...
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bioRxiv - Physiology 2023Quote: ... 1 µM Fura-FF (AAT Bioquest #21028) was used to monitor extra-mitochondrial Ca2+ and 4.8 µM JC-1 (Enzo Life Sciences #52304 ...
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bioRxiv - Neuroscience 2023Quote: ... Oregon Green 488 BAPTA-1 (AAT Bioquest). To do this ...
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bioRxiv - Physiology 2023Quote: ... 1 µM Fura-FF (AAT Bioquest #21028) was used to monitor extra-mitochondrial Ca2+ and 4.8 µM JC-1 (Enzo Life Sciences #52304 ...
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bioRxiv - Microbiology 2023Quote: ... Half of the wells in the plate were stained with eDNA-specific DiTO-1 (1 μM, AAT Bioquest, #17575) for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:250 OxiVisionTM Green peroxide sensor (AAT Bioquest, Cat No ...