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Citations for Cayman Chemical :
1 - 50 of 84 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Recombinant human protein arginine deiminase 4 (PAD4) was from Cayman Chemical, Inc.
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bioRxiv - Cancer Biology 2024Quote: ... 1pmol of DNA oligo was combined with the indicated concentration of recombinant human cGAS protein (Cayman Chemicals) in EMSA buffer (150mM NaCl ...
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bioRxiv - Immunology 2023Quote: 2 µg recombinant human cGAS (Cayman Chemical, #22810) was incubated with 8 µg IgG control or 4H2 ± nucleic acid (1.25 µg PvuII-digested pcDNA3 and 50 µM GTP ...
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bioRxiv - Immunology 2021Quote: ... 500nM and 1uM of human recombinant Histone 3 (Cayman chemical) and 1ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... with or without the addition of human recombinant irisin (Cayman Chemicals, MI, USA) at 250 ng/mL concentration (27) ...
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bioRxiv - Cancer Biology 2020Quote: ... human COX2 protein (Cayman Chemical, 60122, 4ng), and 25μg cell line lysates in RIPA buffer were separated by WES automated gel electrophoresis System (Protein Simple ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with human recombinant irisin (Cayman Chemical CAS NO: 9037-90-5) at different concentrations ranging from 5 to 70 nM dissolved in phosphate buffer saline (PBS).
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bioRxiv - Immunology 2021Quote: ... In vitro stimulation was performed with 500nM of human recombinant Histone 3 (Cayman chemical) and 5ng/ml recombinant human G-CSF (BioLegend) ...
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bioRxiv - Biochemistry 2024Quote: ... Biotin-X-NHS (Biotin-NHS, Cayman Chemical), Sulfo-NHS-LC-Biotin (Sulfo-Biotin-NHS ...
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bioRxiv - Cell Biology 2020Quote: ... biotin switch technique was performed using S-Nitrosylated Protein Detection Kit (Cayman Chemical# 10006518). Immunofluorescence staining was performed following the manufacturer’s protocol and images were captured using Olympus IX51 microscope attached with Olympus DP72 camera ...
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bioRxiv - Molecular Biology 2024Quote: Biotin switch assay was performed using the S-Nitrosylated Protein Detection Kit (Cayman chemical: 10006518) and HEK293T cells ...
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bioRxiv - Cell Biology 2022Quote: Recombinant TEAD4 protein (500 ng) was incubated with 1 mM alkyne palmitoyl-CoA (Cayman Chemical) for 0.5 h in 20mM Tris 8.0 and 100mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Purified human PPP1R15A single repeat peptides (UK2997, UK3098) were labelled with 10-fold molar concentration of fluorescein-5-malamide (16383, Cayman Chemical Company) in the presence of 1 mM TCEP at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... and 2mM biotin (Cayman chemicals) and boiled for 20 minutes ...
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bioRxiv - Microbiology 2019Quote: ... alkyne-labelled palmitic acid (Cayman Chemical) was added to a final concentration of 45 μM ...
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bioRxiv - Immunology 2022Quote: ... Plates were blocked with 5% low-fat milk in PBS and antigens citrullinated with 280mU/ml recombinant human PAD4 (Cayman chemicals) in 50 mM Tris 10 mM CaCl2 1 mM DTT for 3h at 37C ...
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bioRxiv - Cell Biology 2021Quote: Recombinant MCH (Cayman Chemical) was dissolved in sterile ddH2O (1ug/ul ...
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bioRxiv - Immunology 2022Quote: NETs were pre-incubated with A1AT protein from human plasma (Cayman Chemical #24560) 1h before exposure to HBE ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were cultured in 50 IU/mL recombinant human IL-2 (rhIL-2) (Proleukin) and in some experiments 1 μM dasatinib (Cayman Chemical, 11498) or 10 mM NAC (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... ARP (Biotin-containing reagent, Cayman Chemical # 10009350) modification of the remaining ADPr-Ub were carried out in the presence of 1 mM ARP in 0.5 mM acetic acid at room temperature for 60 min [19] ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat. # 10500), 2.3 mM DTT ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant Scl1 proteins were examined for MPO activity using the Myeloperoxidase Inhibitor Screening Assay Kit (700170, Cayman Chemical, Ann Arbor, MI). The proteins were reconstituted in PBS and used in the assay according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... 50nM recombinant HSPA1A (Cayman Chemicals, 22739) or 50nM recombinant GFP (Sino Biological ...
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bioRxiv - Biochemistry 2023Quote: ... Biotin azide (5) was purchased from Cayman Chemical. Azide-SS-biotin (6 ...
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bioRxiv - Biochemistry 2023Quote: ... 50 nM recombinant HSPA1A (Cayman Chemicals, 22739) or 50 nM recombinant GFP (Sino Biological ...
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bioRxiv - Physiology 2024Quote: ... Sections were counterstained with phalloidin labelled with AF-647 (Cayman Chemicals) and mounted in pro-long diamond with DAPI (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Non-labelled amisulpride (MW 369.5 g/mol, >LJ98% purity) was purchased from Cayman Chemicals, UK (#71675-85-9) ...
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bioRxiv - Immunology 2024Quote: ... with fresh stimulation cocktail and fully labelled D-(+)-Glucose-13C6 (Cayman Chemical Company #26707) at a final concentration of 11.1mM ...
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bioRxiv - Immunology 2022Quote: ... Cells were left stimulating for the desired times and 500µM biotin (Cayman chemicals) was added in each well 30 minutes prior to harvest ...
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bioRxiv - Genomics 2023Quote: ... an internal standard containing isotopically-labelled d9-dipalmitoyl phosphatidylcholine (d9-DPPC; Cayman Chemical, MI USA) was added to the samples ...
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bioRxiv - Microbiology 2024Quote: All endogenous and isotope labelled lipid mediator standards were purchased from Cayman Chemical (Ann Arbor, MI, USA). Flax paper (Zig-zac ...
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bioRxiv - Biophysics 2020Quote: ... 17-Octadecynoic Acid (17-ODYA) and biotin-azide were purchased from Cayman Chemicals (Ann Arbor, USA). HeLa cell lines were acquired from ATCC (Manassas ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-human sPLA2-IIA (Cayman Chemical, 160502 ...
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bioRxiv - Molecular Biology 2023Quote: We used the biotin switch method that converts –SNO into biotinylated groups using a detection kit (Cayman Chemical), to detect S-nitrosylation of METTL3 in EndoC-βH1 cells treated with IFN-α and IL-1β according to the protocols of the manufacturer.
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bioRxiv - Immunology 2023Quote: ... 20 µL 10 mM N-[6-(biotinamido)hexyl]-3′-(2′-pyridyl dithio)propionamide (biotin-HPDP, Cayman Chemical Company, 16459) in DMSO was added to each sample ...
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bioRxiv - Neuroscience 2023Quote: ... human sPLA2-IIA ELISA kit (Cayman Chemical, 501380)
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with DMEM containing 2% FBS and labelled with 30 μM 17-ODYA (17-Octadecynoic Acid,34450, Cayman Chemicals) or DMSO for 3hr at 37°C incubator ...
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bioRxiv - Plant Biology 2023Quote: ... Approximate 80 μg of total protein was used for biotin labeling in the presence of 0.4 mM thiol-reactive N-[6-(Biotinamido) hexyl]-3’-(2’-pyridyldithio) propionamide (biotin-HPDP; Cayman Chemical). The sample was then incubated for 60 min at 25°C with mild shaking in the dark followed by acetone precipitation and cleanup ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-human GPR17 (Cayman Chemical, 10136, 1:100), MBP (Rat anti MBP aa82-87 BioRad 1:300) ...
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bioRxiv - Neuroscience 2019Quote: ... Protein content was determined using a Protein Determination Kit (Cayman Chemical, Cat# 704002) and absorbance was measured at 595 nm using a Nanodrop 2000 spectrophotometer (Thermofisher ...
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bioRxiv - Immunology 2022Quote: ... Assays were performed to measure human NE (Cayman Chemical 600610), CG (Cayman Chemical 14993 ...
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bioRxiv - Immunology 2022Quote: ... The Cu(I)-catalyzed click reaction was initiated by adding Click-iT reaction buffer and 50 µl of Azide-PEG3-biotin in 50 mM (Cayman Chemical, 23419) to each sample and incubated on a rotator for 20 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589) in the presence of 50 mM Tris pH-7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples are briefly allowed to cool to room temperature (2-3 min), then working quickly, 1μL 30 mM aldehyde reactive probe (ARP, aminooxy) biotin (22582, Cayman Chemicals, stock made in water) is added first ...
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bioRxiv - Physiology 2023Quote: The ability of plasma to inhibit recombinant HDAC1 was assessed with HDAC1 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... OPP protein synthesis was measured using the O-Propargyl-puromycin (OPP) Protein Synthesis Assay Kit (Cayman Chemical). T cells were incubated in complete T-cell media ...
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bioRxiv - Immunology 2022Quote: ... NETs were pre-incubated with protease inhibitors: human A1AT (Cayman Chemical 24560), sivelestat (Cayman Chemical #177749) ...
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bioRxiv - Neuroscience 2019Quote: ... The amount of protein carbonyls was determined using a Protein Carbonyl Colorimetric Assay Kit (Cayman Chemical, Cat#10005020), per manufacturer instructions and absorbance was measured at a wavelength between 360-385 nm using a plate reader ...
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bioRxiv - Cell Biology 2021Quote: ... Centromere Protein E Inhibitor (GSK923295; Cayman Chemicals), Actin Depolymerizer (Cytocholasin-D ...
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bioRxiv - Neuroscience 2020Quote: Protein carbonyl colorimetric assay kit (Cayman chemical 10005020) was used to assay the carbonyl content in human and mouse brains or hearts ...