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Citations for Cayman Chemical :
501 - 550 of 563 citations for Rat Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: We measured total antioxidant activity in lung tissue homogenates using an Antioxidant Assay Kit according to the manufacturer’s instructions (Item No. 709001; Cayman Chemical). The assay compares the ability of tissue samples to inhibit the oxidation of 2,2’-Azino-di-3-ethylbenzthiazoline sulphonate to Trolox ...
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bioRxiv - Physiology 2024Quote: ... The uptake of fluorescent 2-NBDG was assessed according to manufacturer’s instructions (Cayman Chemicals - Glucose Uptake Cell-Based Assay Kit) on a Synergy HTX plate reader (BioTek).
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bioRxiv - Cancer Biology 2024Quote: ... were incubated for 24 h with SpiD3 and stained using the ROS-detection cell-based assay kit (DCFDA; Cayman Chemical) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Analysis of mitochondrial respiratory chain complex V activity was measured with Complex V Mitocheck kit (Cayman Chemical, Ann Arbor, MI, USA). Mitochondrial extracts (50 μg ...
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bioRxiv - Neuroscience 2021Quote: ... Levels of nitrite/nitrate in plasma were measured using a commercially available kit (Cat. No. 780001, Cayman Chemical, Ann Arbor, MI) as instructed in the manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2019Quote: We obtained one measure of the levels of non-enzymatic antioxidants (Monaghan et al., 2009) by quantifying total antioxidant capacity (TAC) using the TAC kit from Cayman Chemical. Plasma was diluted in assay buffer and assayed according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: We obtained one measure of the levels of enzymatic antioxidants (Monaghan et al., 2009) by quantifying levels of superoxide dismutase (SOD) using the SOD kit from Cayman Chemical. SOD was expressed as units/mg/ml protein (quantified using a Biotek Take3 protocol) ...
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bioRxiv - Cell Biology 2020Quote: ... and tSETD2-Flag (a.a. 1418-2564) was measured over 3-4 hours using a Methyltransferase Fluorescence Assay Kit (Cayman Chemical, 700150), a continuous enzyme-coupled assay that continuously monitors SAM-dependent methyltransferase activity27,28 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cAMP levels were determined by a cAMP enzyme immunoassay kit following the manufacturer’s instructions (Cayman Chemical Co., Ann Arbor, MI, USA)
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bioRxiv - Immunology 2022Quote: Basolateral-to-apical PMN transmigration was quantified by measurement of PMN MPO using a commercial kit (Cayman Chemical, Ann Arbor, MI) as previously described (75) ...
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bioRxiv - Microbiology 2021Quote: The assessment of astodrimer sodium cytotoxicity occurred on Day 4 by measuring lactate dehydrogenase (LDH) activity in the cytoplasm using an LDH detection kit (Cayman Chemical), with 0.5% saponin used as the positive cytotoxic control.
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bioRxiv - Developmental Biology 2020Quote: ... The concentrations of testosterone in the serum samples were measured by using a Testosterone EIA kit (Cayman Chemical Company, Michigan, USA).
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bioRxiv - Microbiology 2021Quote: Phagocytosis assays for testing the phagocytic uptake and route of a substrate in the non-functional and RIDD resistant Blos1 variants in RAW264.7 murine macrophages were performed using the Phagocytosis Assay Kit (IgG FITC) (Cayman Chemical, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Isozyme-specific inhibition study was performed by following the COX Fluorescent Inhibitor Screening Assay Kit (Cayman Chemicals, Ann Arbor, MI, USA) guidelines.59 Shortly ...
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bioRxiv - Neuroscience 2021Quote: ... Aliquots of the medium were collected 15 minutes after stimulation and analyzed for PGE2 content uising an immunoassay kit (Cayman Chemicals) following the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... CAT and GSH-Px in the brain specimens were determined using the commercially available kits (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s procedures (5).
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bioRxiv - Developmental Biology 2021Quote: Catalase (CAT) and superoxide dismutase (SOD) activities were measured using commercially available kits (#707002 and #706002, respectively; Cayman Chemical, MI, USA). After amputation ...
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bioRxiv - Immunology 2021Quote: Neutrophil phagocytic activity was examined by measuring the amount of uptake of latex beads coated with FITC-labeled rabbit IgG into cells using a phagocytosis assay kit (Cayman Chemical) as previously described (21).
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bioRxiv - Cancer Biology 2022Quote: ... The cells were collected by scraping and glutathione (GSH+GSSG) was measured using a Cayman Chemical Glutathione Assay Kit (Cayman Chemical), according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: The ability of plasma to inhibit recombinant HDAC1 was assessed with HDAC1 inhibitor screening assay kit (Cayman Chemical, Ann Arbor, MI) following manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and stored at -70°C until the TXB2 assay was performed using a kit from Cayman Chemicals (Ann Arbor, MI, USA) and a FLUOstar Omega microplate reader (BMG 379 Labtech) ...
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bioRxiv - Physiology 2023Quote: The 2NDBG uptake assay in C2C12 myotubes and mouse primary skeletal myotubes was performed using a commercial assay kit (Cayman chemicals) following previously published protocol (5) ...
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bioRxiv - Cell Biology 2023Quote: ... Glycerol concentrations in the samples were determined using a glycerol colorimetric assay kit (item no. 10010755, Cayman Chemical Company, MI, USA) with an appropriate dilution.
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bioRxiv - Physiology 2023Quote: ... TAG and glycogen levels were quantified in female pelts (five per tube) at 24 h intervals up to 96 h PBM using the Glycogen Assay Kit (Cayman Chemical) and Triglycerides Reagent (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... MTT metabolic assay was performed according to the manufacturer’s protocol (MTT Cell Proliferation Assay Kit (Cayman Chemical, Ann Arbor, MI, USA)) using 4000 and 5000 cells treated or not with etoposide for 21 h ...
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bioRxiv - Physiology 2023Quote: Liver tissues were collected from experimental mice and 20-60mg of tissue was homogenized and quantified for triglyceride content using a colorimetric assay kit from Cayman Chemical. Serum levels of ALT and AST were assessed by the University of Minnesota’s Veterinary Medical Center Clinical Pathology Laboratory
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bioRxiv - Neuroscience 2024Quote: ... and medium (from ex vivo experiments) was collected and used to quantify the concentration of neuropeptide using an enzyme linked immunosorbent kit (CGRP: Cayman Chemicals #589001 ...
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bioRxiv - Physiology 2024Quote: Lipase activity was measured in thawed aliquots of the fat body supernatant using a fluorometric Lipase Activity Assay Kit (Cayman Chemical) as has been previously done with honey bee fat body tissue (Corby-Harris ...
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bioRxiv - Molecular Biology 2024Quote: ... Total S-nitrosylated proteins were measured using an S-nitrosylated Protein Detection Assay Kit (Cayman Chemical Company, Ann Arbor, MI, USA). Both carbonylated and S-nitrosylated proteins were detected using Supersignal West Pico Chemiluminescent substrate (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant Scl1 proteins were examined for MPO activity using the Myeloperoxidase Inhibitor Screening Assay Kit (700170, Cayman Chemical, Ann Arbor, MI). The proteins were reconstituted in PBS and used in the assay according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... or CpG-stimulated patient-derived CLL samples (1e7 cells/mL) treated with inhibitors (24 h) or cycloheximide (30 min) were processed using the Protein Synthesis Assay Kit (Cayman Chemical) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Total glutathione (GSH) levels in the cells were estimated using a glutathione assay kit (Cayman Chemical, 703002, Ann Arbor, MI, USA) by analyzing the absorbance measured after the addition of the assay cocktail reagent mixture to the deproteinated lysate in the 96-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... plasma was isolated from tail blood and measured (3 μL in technical duplicates) using a commercially available kit (Cayman Chemicals #10010303) as per the manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2019Quote: We measured oxidative damage to proteins (Monaghan et al., 2009) using the protein carbonyl colorimetric kit by Cayman Chemical (Ann Arbor, USA). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... An equal amount of mitochondrial fraction was subjected to the MitoCheck Complex I Activity Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). The Complex I activity was determined by calculating the slope of the initial linear portion of the curve according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ALT and AST levels from plasma of mice were measured with the ALT and AST colorimetric activity assay kits (Cayman Chemical, MI). ALT activity was measured by monitoring the rate of NADH oxidation in a coupled reaction using lactate dehydrogenase (LDH) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Oxygen consumption rates of RBL-2H3 mast cells and of primary human keratinocytes were assessed via the Oxygen Consumption Rate Assay Kit (Cayman Chemical Company) as published (Weatherly et al. ...
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bioRxiv - Immunology 2023Quote: The effect of IgG control or 4H2 (0-160 µg) on cGAMP production was assayed using the cGAS Inhibitor Screening Assay Kit (Cayman Chemical, #701930) as per the manufacturer’s instructions except we used half of the recommended quantity of cGAS to allow room to detect signal amplification in the presence of 4H2 ...
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bioRxiv - Cell Biology 2023Quote: ... MDM treated or not as described above were cultured with latex bead-rabbit IgG-FITC complexes (Cayman Chemical phagocytosis assay kit 500290) at 1:300 for 1 h at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The SOD activity of liver homogenate (containing 50 mM MES, pH 6.5, 1 mM EDTA) was determined using commercial SOD assay kit (Cayman Chemical Co., # 66970752). Briefly ...
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bioRxiv - Physiology 2019Quote: The supernatants of each tissue sample were used to analyze malondialdehyde levels (MDA; lipid peroxide parameter) by a TBARS assay kit (Cayman Chemical Co, USA). The MDA level was expressed as nM/mg of protein.
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bioRxiv - Molecular Biology 2021Quote: The activation of cellular oxidative stress in mammalian cells was detected using a ROS detection cell-based assay kit (Cayman Chemical; Cat#: 601290). The fluorescence of DHE staining was visualized using a BZX fluorescent microscope (Keyence) ...
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bioRxiv - Immunology 2020Quote: Cardiac Troponin-I (CTNI) concentration and Alanine Aminotransferase (ALT) activity in the blood were measured by kits per manufacturers’ instructions (Life Diagnostics and Cayman Chemical, respectively). 50ul of whole blood per mouse were collected via retro-orbital bleeding in heparin coated tubes then counted immediately using HemaTrue (HESK).
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bioRxiv - Molecular Biology 2022Quote: Lactate dehydrogenase (LDH) activity in culture medium was measured using a commercially available LDH Cytotoxicity Assay Kit (Cayman Chemical, Ann Arbor, MI, USA) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: Plasma 11-ketotestosterone (11-KT) levels were measured using a commercially available enzyme immunoassay (EIA) kit (Cayman Chemical Company, Ann Arbor, MI, USA) as previously described and validated for this species [23] ...
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bioRxiv - Developmental Biology 2023Quote: Ecdysteroid concentrations in timed egg collections were measured using the 20-Hydroxyecdysone (20E) Enzyme Immunoassay (EIA) kit (Cayman Chemical Company, Ann Arbor, MI) based on the method developed by Porcheron et al ...
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bioRxiv - Neuroscience 2024Quote: ... was collected and used to quantify the concentration of neuropeptide using an enzyme linked immunosorbent kit (CGRP: Cayman Chemicals #589001, Substance P: Cayman Chemicals #583751) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Suspensions were centrifuge at 3,000 g for 5 min and the cAMP levels in supernatants were measured using the cAMP EIA Kit (Cayman Chemical, Ann Arbor, MI, US) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... albumin was measured by radial immunodiffusion assay (RID) and urine creatinine was determined with a Creatinine (urinary) Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI).(64 ...
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bioRxiv - Microbiology 2020Quote: Nile Red fluorescence staining was assessed with the Lipid Droplets Fluorescence Assay Kit according to the manufacturer’s protocol (Cayman Chemical, Ann Arbor, MI, USA). One day before staining assay ...