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Citations for Cayman Chemical :
201 - 245 of 245 citations for NADH Cytochrome B5 Reductase 2 CYB5R2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... The uptake of fluorescent 2-NBDG was assessed according to manufacturer’s instructions (Cayman Chemicals - Glucose Uptake Cell-Based Assay Kit) on a Synergy HTX plate reader (BioTek).
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used in immunohistochemical study: HIF-1α (RRID:AB_409037,1:200,Cayman Chemical #100642,), Sox10 (RRID ...
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bioRxiv - Microbiology 2021Quote: ... Immunoblotting was performed using antibodies against HIF-1α (Cayman Chemical, Ann Arbor, USA, 1:700; 10006421), HIF-2α (Novus Biologicals ...
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bioRxiv - Genomics 2020Quote: ... at 1:1,000 dilution and rabbit HIF-1α (C-Term) Polyclonal Antibody (Cayman Chemical Item 10006421) 1:3,000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... USA), while ponasterone (2β,3β,14α,20β,22R-pentahydroxy-5β-cholest-7-en-6-one, 2) was obtained from Cayman Chemical Company (Ann Arbor ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C in 2% normal goat serum together with mouse anti-nitrotyrosine (1:150; Cayman Chemical). Slides were then washed and incubated in goat anti-mouse biotin secondary antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with the following primary antibodies prepared in antibody diluent (TBS solution with 0.02% Triton X-100 plus 1% bovine serum albumin and 2% normal goat serum): mouse anti-adropin (Cat. No. 14117, 1:200; Cayman Chemical), rat anti-CD31 (Cat ...
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bioRxiv - Pathology 2021Quote: ... cells media was changed to DMEM containing 2% charcoal striped and dialyzed media with 0.1 uM of T0901317 and/or 1uM of LG100268 (noted LG268) (Cayman Chemical, USA). After overnight treatment ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with DMEM containing 2% FBS and labelled with 30 μM 17-ODYA (17-Octadecynoic Acid,34450, Cayman Chemicals) or DMSO for 3hr at 37°C incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... inhibitors were pre-incubated with MB solution 2 h before irradiation: Fer-1 (Cayman Chemical, Ann Arbor, Michigan, EUA, 1 µM); Nec-1 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and seeded at a density of 500,000 cells per well of a matrigel-coated 6-well plate in iPS-Brew XF supplemented with 2 uM Thiazovivin ROCK inhibitor (Cayman Chemical). Definitive endoderm was derived using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... Etomoxir and the KAT2A specific inhibitor cyclopentylidene-[4-(4’-chlorophenyl)thiazol-2-yl)hydrazone (CPTH2)79 were purchased from Cayman Chemical, dissolved in ethanol and used at concentrations of 50 and 2 μM ...
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bioRxiv - Synthetic Biology 2023Quote: Thin films were prepared by dissolving 1mM 1-palmitoyl-2-oleoyl-sn-glycero-3-PC (POPC) and 1 mM cholesterol (Catalog No. 15102, Catalog No. 9003100, Cayman Chemical) in chloroform or dichloromethane in glass vials ...
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bioRxiv - Neuroscience 2020Quote: ... or with a mixture of 25 mM KCl and 5 μM NMDA receptor antagonist D-2-amino-5-phosphopentanoic acid (D-APV, Cayman Chemical Company) to study BDNF autoregulation or neuronal activity-dependent expression of the BDNF gene ...
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bioRxiv - Developmental Biology 2022Quote: ... (gift from Jeremy Nathans (41)) or C3H10T1/2-STF (ATCC CCL-226) cells were treated with 1 µM LGK-974 (Cayman Chemical 14072) for 48 h to inhibit endogenous WNT secretion ...
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bioRxiv - Neuroscience 2022Quote: ... were immobilized in a PDMS microfluidic olfactory chip [58,59] and exposed to either a control buffer or a stimulus buffer containing 100mM 2-AG (Cayman Chemical Co. #62160). The required volume of 2-AG required to make 100mM solution was dissolved in 0.1% ethanol before being added to S-Basal buffer ...
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bioRxiv - Physiology 2023Quote: ... The reaction proceeded for 2 hours at 37°C in presence of either 300 nM MS-275 (Cayman Chemical, Ann Arbor, MI) or vehicle (0.01% DMSO) ...
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bioRxiv - Neuroscience 2024Quote: Visually and electrophysiologically identified layer II-III pyramidal cells were filled with the calcium-sensitive dye Rhod-2 (200 µM, Cayman chemicals, 20777) using patch pipettes ...
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bioRxiv - Neuroscience 2022Quote: ... on Experimental Day 1 and one injection of 5-iodo-2’-deoxyuridine (IdU: 56.75 mg/kg; i.p., Cayman Chemical, Ann Arbor, MI, USA) on Experimental Day 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... and BMS493 was further diluted to a concentration of 2 mg/mL in DPBS supplemented with 0.15M hydroxypropyl β-cyclodextrin (HP-β-CD; Cayman Chemicals 16169), for a total volume of 0.5 mL per dose (1 mg/dose) ...
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bioRxiv - Biochemistry 2022Quote: ... females were anaesthetized and injected (2×69nl) with tyrosine kinase inhibitors (all 200μM) SU6656 (CAS 330161-87-0, Cayman Chemical, Ann Arbor, USA), genistein (CAS 446-72-0 ...
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bioRxiv - Microbiology 2022Quote: To estimate pulmonary oxidative stress we measured oxidized guanosine (8-hydroxyguanosine, 8-hydroxy-2’-deoxyguanosine, and 8-hydroxyguanine) in the lungs of mice using an ELISA (Cayman Chemical cat. 589320) following the manufacturer’s instructions and analysis template.
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bioRxiv - Neuroscience 2024Quote: ... containing the deuterated internal standards 2-arachidonoylglycerol-d5 (100 ng/ml) and arachidonoylethanolamide-d (1 ng/ml) (Cayman Chemical, Ann Arbor, MI, USA). Samples were homogenized and centrifuged on an Eppendorf miniSpin microtube centrifuge at 13,400 rpm for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... tubacin (10 and 20µM; Selleckchem, Houston, TX), Nintedanib (24h 2 µM; Selleckchem, Houston, TX) and PGE2 (96hr, 1 μM, Cayman Chemicals, Ann Arbor, MI) or FGF2 (96 h ...
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bioRxiv - Immunology 2021Quote: ... The concentration of anti-OVA IgG antibodies was determined using Anti-Ovalbumin IgG1 (mouse) ELISA Kit (Cayman Chemical).
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bioRxiv - Immunology 2019Quote: ... The inhibition of MK2 (p38/mitogen-activated protein kinase-activated protein kinase 2) was performed with 5 μM MK2 IV (CAYMAN chemical, Ann Arbor, MI). The inhibition of ADAM-17 (a disintegrin and metalloprotease-17 ...
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bioRxiv - Cancer Biology 2019Quote: ... was cloned into the pAdd2 expression plasmid as described above and expressed in Expi293 cells in the presence of 10 µM Kifunensine (Cayman Chemical, 109944-15-2). After nickel affinity chromatography ...
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bioRxiv - Cell Biology 2019Quote: 2-DG-6-Phosphate phosphatase assays were performed in 250 µL of reaction containing 1.5mM 2-DG-6-Phosphate (#17149, Cayman Chemicals, Ann Arbor, Michigan, USA) in 50 mM HEPES pH 6.7 ...
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bioRxiv - Cell Biology 2020Quote: ... The ROCK1/2 inhibitor Trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride (Y-27632) was obtained from Cayman Chemical (Ann Arbor, MI, USA) and the calcium indicator Fura-2 AM from Life Technologies (Carlsbad ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples are briefly allowed to cool to room temperature (2-3 min), then working quickly, 1μL 30 mM aldehyde reactive probe (ARP, aminooxy) biotin (22582, Cayman Chemicals, stock made in water) is added first ...
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bioRxiv - Neuroscience 2023Quote: HDAC activity of class I HDACs (HDAC1, 2, 3 and 8) was measured with the fluorometric HDAC Activity Assay kit: HDAC1 (10011563, Cayman Chemical, Ann Arbor, MI), HDAC2 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used overnight in 1% skimmed milk solution: anti-p50 (1:1000, 13755, Cayman Chemicals), anti-IκBα (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was supplemented on the different days as follows: Day 0 to Day 2, 10ng/ml bFGF (R&D, Cat. No. 100-18B) and 5 µM LGK (Cayman Chemical Company, Cat. No. 1.800.364.9897); Day 2 to Day 3 for 20 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1-[2-chloro-6-[[(3-iodophenyl)methyl]amino]-9H-purin-9-yl]-1-deoxy-N-methyl-β-D-ribofuranuronamide (2-Chloro-IB-MECA; Cayman Chemicals, 163042-96-4), [2-Amino-4-[3-(trifluoromethyl)phenyl]-3-thienyl] phenylmethanone (VCP 171 ...
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bioRxiv - Microbiology 2023Quote: ... for 12 hours at 37°C/5% CO2 in the presence of vehicle (DMSO) or 2 μM JQ1+ to enhance reactivation (Cayman Chemical CAS: 1268524-70-4). Pools of 6 ganglia were homogenized in 1 ml TriPure isolation reagent (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.18 mM KH2PO4) containing 1 mM CaCl2 or with 0.4 μg/ml BsClpP14 in the presence of 11 μM ADEP1 (Cayman Chemical, A-54556A, ~2-fold molar ratio)11 in the same PBS at 40 °C ...
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bioRxiv - Immunology 2022Quote: Skin samples stored in 700 µL Trizol were homogenized in a Precellys® 2 mL Soft Tissue Homogenizing Ceramic Beads Tube (Cayman Chemical, Ann Arbor, MI, USA) using the Precellys® 24 machine ...
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bioRxiv - Physiology 2023Quote: ... LD211 was used as a selective agonist for MC1-R (compound 28 in the original publication),23 [D-Trp8]-γ-MSH as a selective MC3-R agonist,24 THIQ as a selective MC4-R agonist (Cayman Chemical, USA, # 312637-48-2)25 and PG-901 as a selective MC5-R agonist.26 PG-20N was used as a selective MC5-R antagonist.27 LD211 ...
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bioRxiv - Pathology 2021Quote: ... whereas positive controls always had detectable PrPCWD upon proteinase K digestion and visualization by western blotting using an anti-cervid PrP antibody BAR-224 (Cayman Chemical) conjugated to horseradish peroxidase (HRP).
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with horseradish peroxidase (HRP)—conjugated anti-mouse or anti-rabbit secondary antibodies for 1 h at RT: Goat Anti-Mouse IgG HRP (1:4000, 10004302, Cayman Chemicals) or Goat Anti-Rabbit IgG HRP (1:10.000 ...
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bioRxiv - Cell Biology 2023Quote: ... blocked with blocking solution (1% BSA and 0.3% Triton-X in PBS) and stained with FK2 antibody at a 1:500 dilution (Cayman Chemicals; Milipore) overnight (Klickstein et al. ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed in 0.1M PB and then incubated in their respective secondary antibody (1:2000) with DAPI (1:5000; Cayman Chemical; Cat # 14285) diluted in blocking buffer for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... for 15 min before incubation with the last diluted antibody: rabbit anti-V5-tag (GKPIPNPLLGLDST) conjugated with DyLight 550 at 1:300 dilution (Cayman Chemical, 11261) for another 24 h at room temperature.
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bioRxiv - Bioengineering 2020Quote: Serum antibody concentrations and affinity for the OVA model vaccine were measured using an anti-ovalbumin mouse IgG1 ELISA (Cayman Chemicals, 500830). For time course measurements the serum was diluted 1:1,000 in assay buffer and for post-challenge analysis the serum was diluted 1:100,000 ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed in 0.1M PB and then incubated in their respective secondary antibody diluted at 1:2000 with DAPI (1:5000; Cayman Chemical; Cat 14285) in blocking buffer for 1 hour at room temperature ...