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Citations for Cayman Chemical :
451 - 495 of 495 citations for 7 NITRO 3 4 DIHYDRO 1H QUINOXALIN 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were centrifuged at 10,000 xg for 10 minutes at 4°C and the supernatant was processed (undiluted) according to manufacturer instructions at room temperature (Cayman Chemical, 10010303). Mouse placenta samples (stored at -80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... A metabolic labeling protocol was followed[6] and the experimental HEK293 cells were incubated in the presence of 40µM unphosphorylated 4-thiouridine (Cayman Chemical, CAY16373-5). Total RNA was harvested from the cells using Trizol (Ambion ...
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bioRxiv - Biophysics 2022Quote: ... Modifications in DNA ultrastructure were done to condense or decondense chromatin with the use of 600 nM trichostatin A (TSA) for 4 h (Cayman Chemical Company), to increase euchromatin ...
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bioRxiv - Cancer Biology 2020Quote: ... Aphidicolin (working concentration 0,4 μM) and AZ3146 (working concentration 1 or 2 μM) were purchased from Cayman Chemical; SB203580 (working concentration 10 μM ...
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bioRxiv - Physiology 2022Quote: ... This medium was supplemented with either 2 mM linoleic acid dissolved in ethanol (Cayman Chemicals, catalog no. 9015050) or an equal volume of ethanol ...
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bioRxiv - Molecular Biology 2023Quote: Synthetic maresin-2 (13R, 14S-dihydroxy-4Z, 7Z, 9E, 11E, 16Z, 19Z-docosahexaenoic acid; cat# 16369, Cayman Chemical) were administered by i.p ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pre-treated with the GSK3 inhibitor CHIR99021 (2 µM) (Cat # 13122, Cayman Chemicals, Ann Arbor, USA) for ∼20 days until analysis on Day 40.
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bioRxiv - Genomics 2023Quote: ... Quantitation was done by the external standards calibration of DPPC and PC 16:0/18:2 (Cayman Chemicals) where instrument response for the analyte was measured as a ratio between analytes and internal standard’s peak areas.
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bioRxiv - Genomics 2019Quote: ... SpenRRM-FLAG expressing cells were treated with 10 mM HDAC1/2 inhibitor (Cayman Chemical Company, BRD6688, 1404562-17-9) for 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was performed according to the manufacturer’s recommendation (10009365, Cayman Chemicals). After incubation with the MTT reagent ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-231 or BT-549 cells were treated with 2 μM NVP-BKM120 (Cayman Chemical, CAS #944396-07-0), 5 μM of Afuresertib (GSK2110183 ...
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bioRxiv - Cancer Biology 2023Quote: ... The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was performed according to the manufactureŕs recommendation (10009365; Cayman Chemical). After incubation with the MTT reagent ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... we performed the reaction under identical conditions using 1-NBD-decanoyl-2-decanoyl-sn-Gly (NBD-DAG) (Cayman Chemical) as the substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... age and gender-matched 10-16 week old Tal1-creERT2 control mice and Dnmt3a R878H Tal1-creERT2 mice were treated with tamoxifen (4 mg/kg/day; Cayman Chemical, Ann Arbor, Michigan) for 2 doses ...
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bioRxiv - Cancer Biology 2022Quote: ... using a glutathione assay kit based on Ellman’s reagent (DTNB, 5,5′-dithio-bis-2-nitrobenzoic acid) according to the manufacturer’s protocol (Cayman Chemical, t#703002). Data are presented as DNTB fluorescence normalized to total protein ...
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bioRxiv - Developmental Biology 2022Quote: ... cohorts were pre-treated for 2 hours in mTOR inhibitor (Torin, final concentration 100 nM in fish water, Cayman Chemical). For experiments using rapamycin (LC Labs ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then transferred to a fresh 96 well plate and diluted to 1.0 × 106 cells per ml in hTCM+IL-2 and 0.05 µM of the small molecule inhibitor TSA (Cayman Chemical). The 96 well plate was then spun at 200xg for 7 min and incubated in a tissue culture incubator overnight ...
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bioRxiv - Physiology 2024Quote: ... The uptake of fluorescent 2-NBDG was assessed according to manufacturer’s instructions (Cayman Chemicals - Glucose Uptake Cell-Based Assay Kit) on a Synergy HTX plate reader (BioTek).
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bioRxiv - Physiology 2019Quote: ... N = 4) (all chemicals from Sigma Aldrich, St. Louis, MO, USA, except for the bafilomycin A1 which was from Cayman Chemicals, Ann Arbor, MI, USA). For all treatments ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The cortisol was reconstituted in PBS overnight at 4°C and quantified using the Cortisol ELISA Kit (Item № 500360, Cayman Chemical, Ann Arbor MI, USA) following the kit protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... blots were incubated overnight in a cold room in 1:200 COX-2 (mouse) polyclonal antibody (aa 570-598) from Cayman chemical or 1:1000 monoclonal anti-β-actin antibody produced in mouse (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with the following primary antibodies prepared in antibody diluent (TBS solution with 0.02% Triton X-100 plus 1% bovine serum albumin and 2% normal goat serum): mouse anti-adropin (Cat. No. 14117, 1:200; Cayman Chemical), rat anti-CD31 (Cat ...
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bioRxiv - Pathology 2021Quote: ... cells media was changed to DMEM containing 2% charcoal striped and dialyzed media with 0.1 uM of T0901317 and/or 1uM of LG100268 (noted LG268) (Cayman Chemical, USA). After overnight treatment ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with DMEM containing 2% FBS and labelled with 30 μM 17-ODYA (17-Octadecynoic Acid,34450, Cayman Chemicals) or DMSO for 3hr at 37°C incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... inhibitors were pre-incubated with MB solution 2 h before irradiation: Fer-1 (Cayman Chemical, Ann Arbor, Michigan, EUA, 1 µM); Nec-1 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and seeded at a density of 500,000 cells per well of a matrigel-coated 6-well plate in iPS-Brew XF supplemented with 2 uM Thiazovivin ROCK inhibitor (Cayman Chemical). Definitive endoderm was derived using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... or with a mixture of 25 mM KCl and 5 μM NMDA receptor antagonist D-2-amino-5-phosphopentanoic acid (D-APV, Cayman Chemical Company) to study BDNF autoregulation or neuronal activity-dependent expression of the BDNF gene ...
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bioRxiv - Developmental Biology 2022Quote: ... (gift from Jeremy Nathans (41)) or C3H10T1/2-STF (ATCC CCL-226) cells were treated with 1 µM LGK-974 (Cayman Chemical 14072) for 48 h to inhibit endogenous WNT secretion ...
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bioRxiv - Microbiology 2020Quote: Neutralizing assay was performed using SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA; Item No. 502070) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... were immobilized in a PDMS microfluidic olfactory chip [58,59] and exposed to either a control buffer or a stimulus buffer containing 100mM 2-AG (Cayman Chemical Co. #62160). The required volume of 2-AG required to make 100mM solution was dissolved in 0.1% ethanol before being added to S-Basal buffer ...
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bioRxiv - Physiology 2023Quote: ... The reaction proceeded for 2 hours at 37°C in presence of either 300 nM MS-275 (Cayman Chemical, Ann Arbor, MI) or vehicle (0.01% DMSO) ...
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bioRxiv - Neuroscience 2024Quote: Visually and electrophysiologically identified layer II-III pyramidal cells were filled with the calcium-sensitive dye Rhod-2 (200 µM, Cayman chemicals, 20777) using patch pipettes ...
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bioRxiv - Cancer Biology 2022Quote: ... and BMS493 was further diluted to a concentration of 2 mg/mL in DPBS supplemented with 0.15M hydroxypropyl β-cyclodextrin (HP-β-CD; Cayman Chemicals 16169), for a total volume of 0.5 mL per dose (1 mg/dose) ...
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bioRxiv - Biochemistry 2022Quote: ... females were anaesthetized and injected (2×69nl) with tyrosine kinase inhibitors (all 200μM) SU6656 (CAS 330161-87-0, Cayman Chemical, Ann Arbor, USA), genistein (CAS 446-72-0 ...
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bioRxiv - Microbiology 2022Quote: To estimate pulmonary oxidative stress we measured oxidized guanosine (8-hydroxyguanosine, 8-hydroxy-2’-deoxyguanosine, and 8-hydroxyguanine) in the lungs of mice using an ELISA (Cayman Chemical cat. 589320) following the manufacturer’s instructions and analysis template.
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bioRxiv - Neuroscience 2024Quote: ... containing the deuterated internal standards 2-arachidonoylglycerol-d5 (100 ng/ml) and arachidonoylethanolamide-d (1 ng/ml) (Cayman Chemical, Ann Arbor, MI, USA). Samples were homogenized and centrifuged on an Eppendorf miniSpin microtube centrifuge at 13,400 rpm for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... tubacin (10 and 20µM; Selleckchem, Houston, TX), Nintedanib (24h 2 µM; Selleckchem, Houston, TX) and PGE2 (96hr, 1 μM, Cayman Chemicals, Ann Arbor, MI) or FGF2 (96 h ...
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bioRxiv - Immunology 2019Quote: ... The inhibition of MK2 (p38/mitogen-activated protein kinase-activated protein kinase 2) was performed with 5 μM MK2 IV (CAYMAN chemical, Ann Arbor, MI). The inhibition of ADAM-17 (a disintegrin and metalloprotease-17 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were washed three times at 5 minutes each with TBS-T and were incubated for 2-hours with a 1:10,000 dilution of an appropriate secondary antibody conjugated to horseradish peroxidase in 2% skim milk in TBS-T (Cayman Chemical Company, Ann Arbor, MI). Signal detection was achieved with a Super Signal West Pico Chemiluminescence kit (Pierce Biotechnology ...
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bioRxiv - Cancer Biology 2019Quote: ... was cloned into the pAdd2 expression plasmid as described above and expressed in Expi293 cells in the presence of 10 µM Kifunensine (Cayman Chemical, 109944-15-2). After nickel affinity chromatography ...
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bioRxiv - Cell Biology 2019Quote: 2-DG-6-Phosphate phosphatase assays were performed in 250 µL of reaction containing 1.5mM 2-DG-6-Phosphate (#17149, Cayman Chemicals, Ann Arbor, Michigan, USA) in 50 mM HEPES pH 6.7 ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was supplemented on the different days as follows: Day 0 to Day 2, 10ng/ml bFGF (R&D, Cat. No. 100-18B) and 5 µM LGK (Cayman Chemical Company, Cat. No. 1.800.364.9897); Day 2 to Day 3 for 20 h ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.18 mM KH2PO4) containing 1 mM CaCl2 or with 0.4 μg/ml BsClpP14 in the presence of 11 μM ADEP1 (Cayman Chemical, A-54556A, ~2-fold molar ratio)11 in the same PBS at 40 °C ...
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bioRxiv - Immunology 2022Quote: Skin samples stored in 700 µL Trizol were homogenized in a Precellys® 2 mL Soft Tissue Homogenizing Ceramic Beads Tube (Cayman Chemical, Ann Arbor, MI, USA) using the Precellys® 24 machine ...
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bioRxiv - Physiology 2023Quote: ... LD211 was used as a selective agonist for MC1-R (compound 28 in the original publication),23 [D-Trp8]-γ-MSH as a selective MC3-R agonist,24 THIQ as a selective MC4-R agonist (Cayman Chemical, USA, # 312637-48-2)25 and PG-901 as a selective MC5-R agonist.26 PG-20N was used as a selective MC5-R antagonist.27 LD211 ...