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Citations for Cayman Chemical :
401 - 450 of 470 citations for 5 Fluoro 2 methoxy 4 pyridinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Samples were prepared for HPLC injection by mixing 5 µL of lipid extract with 2.5 µL of an internal standard mixture (all from Cayman Chemicals) consisting of 2.5 ng LPC(O-16:0- D4/0:0) ...
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bioRxiv - Molecular Biology 2023Quote: The remaining compounds were sourced commercially without additional purification: necrostatin 1 and necrostatin 5 (Cayman Chemical, Ann Arbor, MI, USA), necrostatin 1S (MedChemExpress ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: ... then blocked for 60 minutes in PBST containing 5% normal goat serum (NGS) (Cayman Chemical, Cat#10006577, Ann Arbor, MA). Solution was then switched to 5% NGS/PBST containing primary antibodies (see below ...
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bioRxiv - Cancer Biology 2020Quote: ... Aphidicolin (working concentration 0,4 μM) and AZ3146 (working concentration 1 or 2 μM) were purchased from Cayman Chemical; SB203580 (working concentration 10 μM ...
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bioRxiv - Physiology 2022Quote: ... This medium was supplemented with either 2 mM linoleic acid dissolved in ethanol (Cayman Chemicals, catalog no. 9015050) or an equal volume of ethanol ...
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bioRxiv - Molecular Biology 2023Quote: Synthetic maresin-2 (13R, 14S-dihydroxy-4Z, 7Z, 9E, 11E, 16Z, 19Z-docosahexaenoic acid; cat# 16369, Cayman Chemical) were administered by i.p ...
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bioRxiv - Genomics 2023Quote: ... Quantitation was done by the external standards calibration of DPPC and PC 16:0/18:2 (Cayman Chemicals) where instrument response for the analyte was measured as a ratio between analytes and internal standard’s peak areas.
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bioRxiv - Cell Biology 2024Quote: ... Cells were pre-treated with the GSK3 inhibitor CHIR99021 (2 µM) (Cat # 13122, Cayman Chemicals, Ann Arbor, USA) for ∼20 days until analysis on Day 40.
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bioRxiv - Cell Biology 2022Quote: ... cells were grown in YES at 32°C to mid-log phase and treated with 4-amino-1-tert-butyl-3-(3-methylbenzyl)pyrazolo[3,4-3]pyrimidine (3MB-PP1) (Toronto Research Chemical; A602960 or Cayman Chemical; 17860) at a final concentration of 15 µM for 30 min ...
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bioRxiv - Plant Biology 2020Quote: Metazachlor treatment was performed on seedlings grown for 5 days on half MS plates containing 50 or 100 nM metazachlor (Cayman Chemical). Metazachlor was added to the medium from a 100 mM stock in dimethylsulfoxide by using an intermediate diluted stock at 100 μM (extemporarily prepared).
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bioRxiv - Cancer Biology 2022Quote: ... organoid medium was replaced with AP selection medium (colonic differentiation medium lacking CHIR99021 and supplemented with 5 uM Nutlin-3 (Cayman Chemical) to select for organoids with loss of APC and TP53 function ...
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bioRxiv - Immunology 2022Quote: ... Plates were blocked with 5% low-fat milk in PBS and antigens citrullinated with 280mU/ml recombinant human PAD4 (Cayman chemicals) in 50 mM Tris 10 mM CaCl2 1 mM DTT for 3h at 37C ...
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bioRxiv - Neuroscience 2022Quote: ... lysates were diluted 1:5 (cingulate cortex, cerebellum, putamen) or 1:10 (frontal cortex) into assay buffer containing 0.25% sodium taurocholate (Cayman Chemical, 16215), 0.25% triton-X-100 (Thermo Fisher ...
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bioRxiv - Genomics 2019Quote: ... SpenRRM-FLAG expressing cells were treated with 10 mM HDAC1/2 inhibitor (Cayman Chemical Company, BRD6688, 1404562-17-9) for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-231 or BT-549 cells were treated with 2 μM NVP-BKM120 (Cayman Chemical, CAS #944396-07-0), 5 μM of Afuresertib (GSK2110183 ...
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bioRxiv - Biochemistry 2022Quote: ... and [1H,15N]-TROSY-HSQC of the LBD with or without 2 molar equivalents of rosiglitazone (Cayman Chemical #71740). 1D [1H]-NMR spectra were collected using natural abundance proteins that were not isotopically labeled ...
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bioRxiv - Immunology 2023Quote: ... 20 µL 10 mM N-[6-(biotinamido)hexyl]-3′-(2′-pyridyl dithio)propionamide (biotin-HPDP, Cayman Chemical Company, 16459) in DMSO was added to each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Arp 2/3 complex inhibitor (CK-666≤200μM) and CDC42 inhibitor (ML-141≤20μM) were obtained from Cayman Chemical. The PI3K pathway inhibitor (LY294002≤100μM) ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... we performed the reaction under identical conditions using 1-NBD-decanoyl-2-decanoyl-sn-Gly (NBD-DAG) (Cayman Chemical) as the substrate ...
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bioRxiv - Microbiology 2023Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) assay was performed using an MTT assay kit (Cayman Chemical). Cells in 96-well plates were treated with DMSO or desired chemical inhibitors at different concentrations and incubated for desired time ...
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bioRxiv - Biochemistry 2023Quote: Thin films were prepared by dissolving 1-palmitoyl-2-oleoyl-sn-glycero-3-PC (POPC) and cholesterol (Cayman Chemical) in chloroform to create 50 mg/mL solutions that were partitioned into amber glass vials ...
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bioRxiv - Cancer Biology 2022Quote: ... age and gender-matched 10-16 week old Tal1-creERT2 control mice and Dnmt3a R878H Tal1-creERT2 mice were treated with tamoxifen (4 mg/kg/day; Cayman Chemical, Ann Arbor, Michigan) for 2 doses ...
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bioRxiv - Neuroscience 2022Quote: We validated our physiologically compatible THC solution (1:1:18 THC:cremophor:saline at 2.5, 5, or 10 mg/kg of THC; Cayman Chemicals, Ann Arbor, MI, USA) by measuring plasma levels of THC and two THC metabolites (11-hydroxy-delta-9-THC and 11nor-9carboxy delta-9-THC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The experiment was stopped by the addition of 5% trichloroacetic acid (TCA) and the concentration of cGMP was measured using the cGMP enzyme immuno assay (EIA) kit (Cayman Chemical Company).
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bioRxiv - Immunology 2022Quote: ... MAIT cells were stimulated by APCs pulsed 3 h with 5-OP-RU as previously described (83) or with 30 µM 6,7-dimethyl-8-Ribityllumazine (Cayman Chemical Cat#23370).
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bioRxiv - Biochemistry 2023Quote: ... cell lysates were incubated for 5 min with competitor (Arachidonic acid, docosanoic acid, Cayman Chemical 9000338, or 8-HETE, Cayman Chemical 34340) at 37°C before addition of other substrates and initiation with NADPH.
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bioRxiv - Biochemistry 2023Quote: Purified human PPP1R15A single repeat peptides (UK2997, UK3098) were labelled with 10-fold molar concentration of fluorescein-5-malamide (16383, Cayman Chemical Company) in the presence of 1 mM TCEP at 4 °C overnight ...
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bioRxiv - Bioengineering 2021Quote: 1-Palmitylthio-2-palmitoylamido-1,2-dideoxy-sn-glycero-3-phosphorylcholine (thioetheramide-PC) was purchased from Cayman Chemical (Ann Arbor, Michigan). Hydrogenated soy phosphatidylcholine (HSPC) ...
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bioRxiv - Cancer Biology 2022Quote: ... using a glutathione assay kit based on Ellman’s reagent (DTNB, 5,5′-dithio-bis-2-nitrobenzoic acid) according to the manufacturer’s protocol (Cayman Chemical, t#703002). Data are presented as DNTB fluorescence normalized to total protein ...
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bioRxiv - Cell Biology 2020Quote: ... PLD assay reagent amplex red 10-Acetyl-3,7-dihydroxyphenoxazine and 2-dioctanoyl-sn-glycero-3-phosphocholine (C8-PC) were purchased from Cayman Chemical. Horseradish peroxidase and choline oxidase were purchased from VWR ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Level of 2′3′-cGAMP within neural cell lysates after oligomeric Aβ42 treatment was measured by 2′3′-cGAMP competitive ELISA kit (501700, Cayman Chemical) accordingly to the manufacture’s instruction.
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bioRxiv - Developmental Biology 2022Quote: ... cohorts were pre-treated for 2 hours in mTOR inhibitor (Torin, final concentration 100 nM in fish water, Cayman Chemical). For experiments using rapamycin (LC Labs ...
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bioRxiv - Plant Biology 2023Quote: ... Approximate 80 μg of total protein was used for biotin labeling in the presence of 0.4 mM thiol-reactive N-[6-(Biotinamido) hexyl]-3’-(2’-pyridyldithio) propionamide (biotin-HPDP; Cayman Chemical). The sample was then incubated for 60 min at 25°C with mild shaking in the dark followed by acetone precipitation and cleanup ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then transferred to a fresh 96 well plate and diluted to 1.0 × 106 cells per ml in hTCM+IL-2 and 0.05 µM of the small molecule inhibitor TSA (Cayman Chemical). The 96 well plate was then spun at 200xg for 7 min and incubated in a tissue culture incubator overnight ...
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bioRxiv - Physiology 2024Quote: ... The uptake of fluorescent 2-NBDG was assessed according to manufacturer’s instructions (Cayman Chemicals - Glucose Uptake Cell-Based Assay Kit) on a Synergy HTX plate reader (BioTek).
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bioRxiv - Physiology 2019Quote: ... N = 4) (all chemicals from Sigma Aldrich, St. Louis, MO, USA, except for the bafilomycin A1 which was from Cayman Chemicals, Ann Arbor, MI, USA). For all treatments ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The cortisol was reconstituted in PBS overnight at 4°C and quantified using the Cortisol ELISA Kit (Item № 500360, Cayman Chemical, Ann Arbor MI, USA) following the kit protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... blots were incubated overnight in a cold room in 1:200 COX-2 (mouse) polyclonal antibody (aa 570-598) from Cayman chemical or 1:1000 monoclonal anti-β-actin antibody produced in mouse (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... USA), while ponasterone (2β,3β,14α,20β,22R-pentahydroxy-5β-cholest-7-en-6-one, 2) was obtained from Cayman Chemical Company (Ann Arbor ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with the following primary antibodies prepared in antibody diluent (TBS solution with 0.02% Triton X-100 plus 1% bovine serum albumin and 2% normal goat serum): mouse anti-adropin (Cat. No. 14117, 1:200; Cayman Chemical), rat anti-CD31 (Cat ...
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bioRxiv - Pathology 2021Quote: ... cells media was changed to DMEM containing 2% charcoal striped and dialyzed media with 0.1 uM of T0901317 and/or 1uM of LG100268 (noted LG268) (Cayman Chemical, USA). After overnight treatment ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with DMEM containing 2% FBS and labelled with 30 μM 17-ODYA (17-Octadecynoic Acid,34450, Cayman Chemicals) or DMSO for 3hr at 37°C incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... inhibitors were pre-incubated with MB solution 2 h before irradiation: Fer-1 (Cayman Chemical, Ann Arbor, Michigan, EUA, 1 µM); Nec-1 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and seeded at a density of 500,000 cells per well of a matrigel-coated 6-well plate in iPS-Brew XF supplemented with 2 uM Thiazovivin ROCK inhibitor (Cayman Chemical). Definitive endoderm was derived using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: Thin films were prepared by dissolving 1mM 1-palmitoyl-2-oleoyl-sn-glycero-3-PC (POPC) and 1 mM cholesterol (Catalog No. 15102, Catalog No. 9003100, Cayman Chemical) in chloroform or dichloromethane in glass vials ...
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bioRxiv - Molecular Biology 2020Quote: ... Suspensions were centrifuge at 3,000 g for 5 min and the cAMP levels in supernatants were measured using the cAMP EIA Kit (Cayman Chemical, Ann Arbor, MI, US) according to the manufacturer’s instructions ...
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Ventral tegmental area glutamate neurons mediate the nonassociative consequences of traumatic stressbioRxiv - Neuroscience 2021Quote: ... Baseline firing in response to current injections was collected for 5 min followed by a 10 min bath application of 10 μM clozapine (Cayman Chemical, Ann Arbor, MI). The average number of action potentials generated during five consecutive sweeps was used to quantify baseline activity and the aver-age of five consecutive sweeps during clozapine application was used to quantify firing during hM4Di receptor activation ...
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bioRxiv - Biophysics 2022Quote: ... were induced along the PM of cultured HeLa cells by adding 10-15% ethanol (25) or 5 µM cytochalasin D (Cayman Chemical, Ann Arbor, MI) to the cell growth medium and incubating for ∼15 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were washed for 3 h and incubated for 30 min (37 °C, 5% CO2) with either 25 µM spermine-NONOate (a nitric oxide donor; Cayman Chemical, cat. no. 82150) or 25 µM sulpho-NONOate (control compound ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were then injected 30 minutes prior to behavioral experiments intraperitoneally with 5 mg/kg of the iDREADD agonist clozapine-n-oxide (CNO; Cayman Chemical, #34233-69-7) and returned to their homecage until behavioral testing ...