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Citations for Cayman Chemical :
451 - 495 of 495 citations for 10 Undecenamide N N bis 2 hydroxyethyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 55 μL of samples (4 μg protein/μL) and standards (Item No. 780014) were incubated with 10 μL each of nitrate reductase cofactors (Item No. 780012; Cayman Chemicals) and enzymes (Item No ...
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bioRxiv - Cell Biology 2020Quote: ... and that was supplemented with 10 μM of ROCK inhibitor and 50 ng/ml of DAPT (Notch signalling inhibitor; Cayman Chemicals). Although we initially established spheroid cultures from a laboratory rabbit (duodenum ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase activity of focal adhesion kinase (FAK) was inhibited by treating the cells with 10 μM PF573228 (Cayman Chemical: 14924)51 at 37°C for 10 min.
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bioRxiv - Immunology 2022Quote: For the pathway studies neutrophils were incubated for 30 min before stimulation with 10 μM BB-Cl-amidine (PADi, Cayman Chemicals), 15 μM Dipenyleneiodium (DPI ...
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bioRxiv - Neuroscience 2022Quote: ... lysates were diluted 1:5 (cingulate cortex, cerebellum, putamen) or 1:10 (frontal cortex) into assay buffer containing 0.25% sodium taurocholate (Cayman Chemical, 16215), 0.25% triton-X-100 (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... SV fusion during stimulation was measured by stimulating sypHy-transfected neurons (10 Hz, 90 s) in the presence of 1 µM bafilomycin A1 (Cayman Chemical Company ...
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bioRxiv - Cell Biology 2023Quote: ... P53 knock-out status of clones was confirmed by testing sensitivity for the p53 stabilizer Nutlin-3a (10 µM for 16h, Cayman Chemical) and Western Blotting for p53 protein.
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bioRxiv - Neuroscience 2023Quote: ... Cells were irradiated with a single dose of 4 Gy in the presence of QTP (10 µM) and treated either with zaragozic acid (squalene synthase inhibitor; #17452, Cayman Chemical,), GGTI-298 (GGTase inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... and seeded on 1:30 Matrigel in 12-well plates in TeSR-E8 media supplemented with Y-27632 (10 μM, #10005583, Cayman Chemical). Media was changed to fresh TeSR-E8 after 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... at a density of 30 000 cells/cm2 in a neural precursor cell differentiation medium (NPCM) supplemented with 10 μM Y-27632 (Cayman Chemicals) and 0.1 μM compound E (Cayman Chemicals) ...
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bioRxiv - Biochemistry 2023Quote: ... The G quadruplex/hemin RNAzyme was allowed to react with 10-Acetyl-3,7-dihydroxyphenoxazine (Amplex Red, Cayman Chemical Company, Cat#10010469) and Hydrogen peroxide for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Medium for AF cells after seeding and organoid cultures after splitting additionally contained ROCK inhibitor Y-27632 (10 μM, Cayman Chemical). The AFKO cultures were split on average every 16 days ...
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bioRxiv - Cell Biology 2023Quote: ... Single-cell suspensions were resuspended in 100–200 µl (PBS) containing 2.5 μg/ml propidium iodide (PI) (Cayman Chemical #14289-10), for 10 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... on Experimental Day 1 and one injection of 5-iodo-2’-deoxyuridine (IdU: 56.75 mg/kg; i.p., Cayman Chemical, Ann Arbor, MI, USA) on Experimental Day 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... and BMS493 was further diluted to a concentration of 2 mg/mL in DPBS supplemented with 0.15M hydroxypropyl β-cyclodextrin (HP-β-CD; Cayman Chemicals 16169), for a total volume of 0.5 mL per dose (1 mg/dose) ...
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bioRxiv - Biochemistry 2022Quote: ... females were anaesthetized and injected (2×69nl) with tyrosine kinase inhibitors (all 200μM) SU6656 (CAS 330161-87-0, Cayman Chemical, Ann Arbor, USA), genistein (CAS 446-72-0 ...
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bioRxiv - Microbiology 2022Quote: To estimate pulmonary oxidative stress we measured oxidized guanosine (8-hydroxyguanosine, 8-hydroxy-2’-deoxyguanosine, and 8-hydroxyguanine) in the lungs of mice using an ELISA (Cayman Chemical cat. 589320) following the manufacturer’s instructions and analysis template.
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bioRxiv - Neuroscience 2024Quote: ... containing the deuterated internal standards 2-arachidonoylglycerol-d5 (100 ng/ml) and arachidonoylethanolamide-d (1 ng/ml) (Cayman Chemical, Ann Arbor, MI, USA). Samples were homogenized and centrifuged on an Eppendorf miniSpin microtube centrifuge at 13,400 rpm for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were centrifuged at 10,000 xg for 10 minutes at 4°C and the supernatant was processed (undiluted) according to manufacturer instructions at room temperature (Cayman Chemical, 10010303).
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were centrifuged at 10,000 xg for 10 minutes at 4°C and the supernatant was processed (undiluted) according to manufacturer instructions at room temperature (Cayman Chemical, 10010303). Mouse placenta samples (stored at -80°C ...
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bioRxiv - Neuroscience 2022Quote: We validated our physiologically compatible THC solution (1:1:18 THC:cremophor:saline at 2.5, 5, or 10 mg/kg of THC; Cayman Chemicals, Ann Arbor, MI, USA) by measuring plasma levels of THC and two THC metabolites (11-hydroxy-delta-9-THC and 11nor-9carboxy delta-9-THC ...
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bioRxiv - Physiology 2021Quote: ... neurons cultured on glass coverslips were incubated in the presence of 10 μg/ml propidium iodide (PI) (Cayman Chemical, Ann Arbor, USA) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Biochemistry 2023Quote: Purified human PPP1R15A single repeat peptides (UK2997, UK3098) were labelled with 10-fold molar concentration of fluorescein-5-malamide (16383, Cayman Chemical Company) in the presence of 1 mM TCEP at 4 °C overnight ...
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: The same amount of RNP mentioned above was firstly premixed and then added to 1/10 volume of premixed SM102-LNP (Cayman Chemical, USA) according to the manufacturer’s instruction for the mRNA loading ...
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bioRxiv - Immunology 2019Quote: ... The inhibition of MK2 (p38/mitogen-activated protein kinase-activated protein kinase 2) was performed with 5 μM MK2 IV (CAYMAN chemical, Ann Arbor, MI). The inhibition of ADAM-17 (a disintegrin and metalloprotease-17 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were washed three times at 5 minutes each with TBS-T and were incubated for 2-hours with a 1:10,000 dilution of an appropriate secondary antibody conjugated to horseradish peroxidase in 2% skim milk in TBS-T (Cayman Chemical Company, Ann Arbor, MI). Signal detection was achieved with a Super Signal West Pico Chemiluminescence kit (Pierce Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: 2-DG-6-Phosphate phosphatase assays were performed in 250 µL of reaction containing 1.5mM 2-DG-6-Phosphate (#17149, Cayman Chemicals, Ann Arbor, Michigan, USA) in 50 mM HEPES pH 6.7 ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples are briefly allowed to cool to room temperature (2-3 min), then working quickly, 1μL 30 mM aldehyde reactive probe (ARP, aminooxy) biotin (22582, Cayman Chemicals, stock made in water) is added first ...
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bioRxiv - Neuroscience 2023Quote: HDAC activity of class I HDACs (HDAC1, 2, 3 and 8) was measured with the fluorometric HDAC Activity Assay kit: HDAC1 (10011563, Cayman Chemical, Ann Arbor, MI), HDAC2 ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was supplemented on the different days as follows: Day 0 to Day 2, 10ng/ml bFGF (R&D, Cat. No. 100-18B) and 5 µM LGK (Cayman Chemical Company, Cat. No. 1.800.364.9897); Day 2 to Day 3 for 20 h ...
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bioRxiv - Microbiology 2023Quote: ... for 12 hours at 37°C/5% CO2 in the presence of vehicle (DMSO) or 2 μM JQ1+ to enhance reactivation (Cayman Chemical CAS: 1268524-70-4). Pools of 6 ganglia were homogenized in 1 ml TriPure isolation reagent (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then resuspended in 0.5 ml BG11 and incubated with 10 μl calcein-AM (Cayman Chemical, 1 mg ml−1 in DMSO) for 1 h at 30 °C in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... These concentrations ensure that a sufficient number of wells contain a single cell.12 E2 (10 ng/mL, Cayman Chemical, Ann Arbor, MI) or OSM (100 ng/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Spheroids of the small intestine were cultured and expanded in conditioned medium supplemented with 10 μM of Rho-associated protein kinase (ROCK) inhibitor (Y-27632; Cayman Chemicals, Michigan, USA) and 10 μM of transforming growth factor-β (TGF-β ...
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bioRxiv - Developmental Biology 2021Quote: ... aNSCs were cultured for 1 d and the medium was supplemented either with 10 μM GW4869 (Cayman Chemical Co., Ann Arbor, MI, USA) or dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... were filled up to 2 mL methanol and 1 mL PBS was added containing deuterium-labelled internal standards (200 nM d8-5S-HETE, d4-LTB4, d5-LXA4, d5-RvD2, d4-PGE2 and 10 μM d8-AA; Cayman Chemical/Biomol GmbH) to facilitate quantification and sample recovery ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.18 mM KH2PO4) containing 1 mM CaCl2 or with 0.4 μg/ml BsClpP14 in the presence of 11 μM ADEP1 (Cayman Chemical, A-54556A, ~2-fold molar ratio)11 in the same PBS at 40 °C ...
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bioRxiv - Immunology 2022Quote: Skin samples stored in 700 µL Trizol were homogenized in a Precellys® 2 mL Soft Tissue Homogenizing Ceramic Beads Tube (Cayman Chemical, Ann Arbor, MI, USA) using the Precellys® 24 machine ...
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bioRxiv - Physiology 2023Quote: ... LD211 was used as a selective agonist for MC1-R (compound 28 in the original publication),23 [D-Trp8]-γ-MSH as a selective MC3-R agonist,24 THIQ as a selective MC4-R agonist (Cayman Chemical, USA, # 312637-48-2)25 and PG-901 as a selective MC5-R agonist.26 PG-20N was used as a selective MC5-R antagonist.27 LD211 ...
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bioRxiv - Microbiology 2021Quote: ... The fixed cells were pelleted by centrifugation for 1 min at 4000 rpm then resuspended in PBS with 10 μg/mL Hoechst 33342 (Cayman Chemical; Item no. 15542). Aliquots of stained cells were loaded onto glass microscope slides and visualized using an Olympus IX70 Epifluorescent Microscope fitted with a Hamamatsu C11440 camera.
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bioRxiv - Biophysics 2022Quote: ... were induced along the PM of cultured HeLa cells by adding 10-15% ethanol (25) or 5 µM cytochalasin D (Cayman Chemical, Ann Arbor, MI) to the cell growth medium and incubating for ∼15 min at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... 1mL of the AggreWell medium was manually replaced with supplemented medium containing WNT inhibitor (IWR1-ε, 3 µM, Cayman Chemical, 13659, days 1-10) and Nodal/Activin inhibitor (SB431542 ...
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bioRxiv - Cancer Biology 2022Quote: ... Trp53 and Smad4 mutations were selected by removing L-WRN conditioned media and adding 10 µm Nutlin-3 (an Mdm2 inhibitor enabling functional selection for Trp53 loss of function) (Cayman Chemical Company #10004372-1). 10 subclones were picked from the engineered bulk organoids ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: Phalloidin staining at E13.5 coronal sections was performed by incubating 1X Phalloidin working solution (1X PBS, 10% BSA and Phalloidin-iFluor™ 488) 1000x conjugate in DMSO (1:1000; Cayman Chemical Company 20549) for 45 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... were first mixed with 2 mL of ice-cold methanol containing deuterium-labelled internal standards (200 nM d8-5S-HETE, d4-LTB4, d5-LXA4, d5-RvD2, d4-PGE2 and 10 μM d8-AA; Cayman Chemical/Biomol GmbH, Hamburg, Germany) to facilitate quantification and sample recovery ...