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Citations for Cayman Chemical :
201 - 250 of 254 citations for Caspase 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 20 µL 10 mM N-[6-(biotinamido)hexyl]-3′-(2′-pyridyl dithio)propionamide (biotin-HPDP, Cayman Chemical Company, 16459) in DMSO was added to each sample ...
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bioRxiv - Microbiology 2023Quote: DDD85646/IMP-366(2,6-dichloro-4-[2-(1-piperazinyl)-4-pyridinyl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)-benzenesulfonamide) was purchased from Cayman Chemicals and used as an inhibitor of viral assembly at the final concentration of 5 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was performed according to the manufactureŕs recommendation (10009365; Cayman Chemical). After incubation with the MTT reagent ...
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bioRxiv - Cancer Biology 2022Quote: ... Arp 2/3 complex inhibitor (CK-666≤200μM) and CDC42 inhibitor (ML-141≤20μM) were obtained from Cayman Chemical. The PI3K pathway inhibitor (LY294002≤100μM) ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... we performed the reaction under identical conditions using 1-NBD-decanoyl-2-decanoyl-sn-Gly (NBD-DAG) (Cayman Chemical) as the substrate ...
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bioRxiv - Microbiology 2023Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) assay was performed using an MTT assay kit (Cayman Chemical). Cells in 96-well plates were treated with DMSO or desired chemical inhibitors at different concentrations and incubated for desired time ...
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bioRxiv - Biochemistry 2023Quote: Thin films were prepared by dissolving 1-palmitoyl-2-oleoyl-sn-glycero-3-PC (POPC) and cholesterol (Cayman Chemical) in chloroform to create 50 mg/mL solutions that were partitioned into amber glass vials ...
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bioRxiv - Neuroscience 2022Quote: ... for 15 min before incubation with the last diluted antibody: rabbit anti-V5-tag (GKPIPNPLLGLDST) conjugated with DyLight 550 at 1:300 dilution (Cayman Chemical, 11261) for another 24 h at room temperature.
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bioRxiv - Cell Biology 2023Quote: ... MDM treated or not as described above were cultured with latex bead-rabbit IgG-FITC complexes (Cayman Chemical phagocytosis assay kit 500290) at 1:300 for 1 h at 37°C ...
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bioRxiv - Bioengineering 2021Quote: 1-Palmitylthio-2-palmitoylamido-1,2-dideoxy-sn-glycero-3-phosphorylcholine (thioetheramide-PC) was purchased from Cayman Chemical (Ann Arbor, Michigan). Hydrogenated soy phosphatidylcholine (HSPC) ...
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bioRxiv - Cancer Biology 2022Quote: ... using a glutathione assay kit based on Ellman’s reagent (DTNB, 5,5′-dithio-bis-2-nitrobenzoic acid) according to the manufacturer’s protocol (Cayman Chemical, t#703002). Data are presented as DNTB fluorescence normalized to total protein ...
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bioRxiv - Cell Biology 2020Quote: ... PLD assay reagent amplex red 10-Acetyl-3,7-dihydroxyphenoxazine and 2-dioctanoyl-sn-glycero-3-phosphocholine (C8-PC) were purchased from Cayman Chemical. Horseradish peroxidase and choline oxidase were purchased from VWR ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Level of 2′3′-cGAMP within neural cell lysates after oligomeric Aβ42 treatment was measured by 2′3′-cGAMP competitive ELISA kit (501700, Cayman Chemical) accordingly to the manufacture’s instruction.
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bioRxiv - Developmental Biology 2022Quote: ... cohorts were pre-treated for 2 hours in mTOR inhibitor (Torin, final concentration 100 nM in fish water, Cayman Chemical). For experiments using rapamycin (LC Labs ...
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bioRxiv - Plant Biology 2023Quote: ... Approximate 80 μg of total protein was used for biotin labeling in the presence of 0.4 mM thiol-reactive N-[6-(Biotinamido) hexyl]-3’-(2’-pyridyldithio) propionamide (biotin-HPDP; Cayman Chemical). The sample was then incubated for 60 min at 25°C with mild shaking in the dark followed by acetone precipitation and cleanup ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then transferred to a fresh 96 well plate and diluted to 1.0 × 106 cells per ml in hTCM+IL-2 and 0.05 µM of the small molecule inhibitor TSA (Cayman Chemical). The 96 well plate was then spun at 200xg for 7 min and incubated in a tissue culture incubator overnight ...
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bioRxiv - Physiology 2024Quote: ... The uptake of fluorescent 2-NBDG was assessed according to manufacturer’s instructions (Cayman Chemicals - Glucose Uptake Cell-Based Assay Kit) on a Synergy HTX plate reader (BioTek).
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bioRxiv - Neuroscience 2023Quote: ... the media was changed into fresh HMC3 media containing fluorescent latex beads (1:500, latex -eads-rabbit IgG-FITC complex - Cayman chemical cat#500290). The cells were incubated for 30 min at 37°C before being washed as the manufacturer protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... blots were incubated overnight in a cold room in 1:200 COX-2 (mouse) polyclonal antibody (aa 570-598) from Cayman chemical or 1:1000 monoclonal anti-β-actin antibody produced in mouse (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... USA), while ponasterone (2β,3β,14α,20β,22R-pentahydroxy-5β-cholest-7-en-6-one, 2) was obtained from Cayman Chemical Company (Ann Arbor ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C in 2% normal goat serum together with mouse anti-nitrotyrosine (1:150; Cayman Chemical). Slides were then washed and incubated in goat anti-mouse biotin secondary antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with the following primary antibodies prepared in antibody diluent (TBS solution with 0.02% Triton X-100 plus 1% bovine serum albumin and 2% normal goat serum): mouse anti-adropin (Cat. No. 14117, 1:200; Cayman Chemical), rat anti-CD31 (Cat ...
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bioRxiv - Pathology 2021Quote: ... cells media was changed to DMEM containing 2% charcoal striped and dialyzed media with 0.1 uM of T0901317 and/or 1uM of LG100268 (noted LG268) (Cayman Chemical, USA). After overnight treatment ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with DMEM containing 2% FBS and labelled with 30 μM 17-ODYA (17-Octadecynoic Acid,34450, Cayman Chemicals) or DMSO for 3hr at 37°C incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... inhibitors were pre-incubated with MB solution 2 h before irradiation: Fer-1 (Cayman Chemical, Ann Arbor, Michigan, EUA, 1 µM); Nec-1 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and seeded at a density of 500,000 cells per well of a matrigel-coated 6-well plate in iPS-Brew XF supplemented with 2 uM Thiazovivin ROCK inhibitor (Cayman Chemical). Definitive endoderm was derived using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... Etomoxir and the KAT2A specific inhibitor cyclopentylidene-[4-(4’-chlorophenyl)thiazol-2-yl)hydrazone (CPTH2)79 were purchased from Cayman Chemical, dissolved in ethanol and used at concentrations of 50 and 2 μM ...
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bioRxiv - Synthetic Biology 2023Quote: Thin films were prepared by dissolving 1mM 1-palmitoyl-2-oleoyl-sn-glycero-3-PC (POPC) and 1 mM cholesterol (Catalog No. 15102, Catalog No. 9003100, Cayman Chemical) in chloroform or dichloromethane in glass vials ...
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bioRxiv - Neuroscience 2020Quote: ... or with a mixture of 25 mM KCl and 5 μM NMDA receptor antagonist D-2-amino-5-phosphopentanoic acid (D-APV, Cayman Chemical Company) to study BDNF autoregulation or neuronal activity-dependent expression of the BDNF gene ...
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bioRxiv - Developmental Biology 2022Quote: ... (gift from Jeremy Nathans (41)) or C3H10T1/2-STF (ATCC CCL-226) cells were treated with 1 µM LGK-974 (Cayman Chemical 14072) for 48 h to inhibit endogenous WNT secretion ...
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bioRxiv - Microbiology 2020Quote: Neutralizing assay was performed using SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA; Item No. 502070) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... were immobilized in a PDMS microfluidic olfactory chip [58,59] and exposed to either a control buffer or a stimulus buffer containing 100mM 2-AG (Cayman Chemical Co. #62160). The required volume of 2-AG required to make 100mM solution was dissolved in 0.1% ethanol before being added to S-Basal buffer ...
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bioRxiv - Physiology 2023Quote: ... The reaction proceeded for 2 hours at 37°C in presence of either 300 nM MS-275 (Cayman Chemical, Ann Arbor, MI) or vehicle (0.01% DMSO) ...
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bioRxiv - Neuroscience 2024Quote: Visually and electrophysiologically identified layer II-III pyramidal cells were filled with the calcium-sensitive dye Rhod-2 (200 µM, Cayman chemicals, 20777) using patch pipettes ...
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bioRxiv - Neuroscience 2022Quote: ... on Experimental Day 1 and one injection of 5-iodo-2’-deoxyuridine (IdU: 56.75 mg/kg; i.p., Cayman Chemical, Ann Arbor, MI, USA) on Experimental Day 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... and BMS493 was further diluted to a concentration of 2 mg/mL in DPBS supplemented with 0.15M hydroxypropyl β-cyclodextrin (HP-β-CD; Cayman Chemicals 16169), for a total volume of 0.5 mL per dose (1 mg/dose) ...
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bioRxiv - Biochemistry 2022Quote: ... females were anaesthetized and injected (2×69nl) with tyrosine kinase inhibitors (all 200μM) SU6656 (CAS 330161-87-0, Cayman Chemical, Ann Arbor, USA), genistein (CAS 446-72-0 ...
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bioRxiv - Microbiology 2022Quote: To estimate pulmonary oxidative stress we measured oxidized guanosine (8-hydroxyguanosine, 8-hydroxy-2’-deoxyguanosine, and 8-hydroxyguanine) in the lungs of mice using an ELISA (Cayman Chemical cat. 589320) following the manufacturer’s instructions and analysis template.
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bioRxiv - Neuroscience 2024Quote: ... containing the deuterated internal standards 2-arachidonoylglycerol-d5 (100 ng/ml) and arachidonoylethanolamide-d (1 ng/ml) (Cayman Chemical, Ann Arbor, MI, USA). Samples were homogenized and centrifuged on an Eppendorf miniSpin microtube centrifuge at 13,400 rpm for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... tubacin (10 and 20µM; Selleckchem, Houston, TX), Nintedanib (24h 2 µM; Selleckchem, Houston, TX) and PGE2 (96hr, 1 μM, Cayman Chemicals, Ann Arbor, MI) or FGF2 (96 h ...
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bioRxiv - Immunology 2019Quote: ... The inhibition of MK2 (p38/mitogen-activated protein kinase-activated protein kinase 2) was performed with 5 μM MK2 IV (CAYMAN chemical, Ann Arbor, MI). The inhibition of ADAM-17 (a disintegrin and metalloprotease-17 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were washed three times at 5 minutes each with TBS-T and were incubated for 2-hours with a 1:10,000 dilution of an appropriate secondary antibody conjugated to horseradish peroxidase in 2% skim milk in TBS-T (Cayman Chemical Company, Ann Arbor, MI). Signal detection was achieved with a Super Signal West Pico Chemiluminescence kit (Pierce Biotechnology ...
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bioRxiv - Cancer Biology 2019Quote: ... was cloned into the pAdd2 expression plasmid as described above and expressed in Expi293 cells in the presence of 10 µM Kifunensine (Cayman Chemical, 109944-15-2). After nickel affinity chromatography ...
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bioRxiv - Cell Biology 2019Quote: 2-DG-6-Phosphate phosphatase assays were performed in 250 µL of reaction containing 1.5mM 2-DG-6-Phosphate (#17149, Cayman Chemicals, Ann Arbor, Michigan, USA) in 50 mM HEPES pH 6.7 ...
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bioRxiv - Cell Biology 2020Quote: ... The ROCK1/2 inhibitor Trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride (Y-27632) was obtained from Cayman Chemical (Ann Arbor, MI, USA) and the calcium indicator Fura-2 AM from Life Technologies (Carlsbad ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples are briefly allowed to cool to room temperature (2-3 min), then working quickly, 1μL 30 mM aldehyde reactive probe (ARP, aminooxy) biotin (22582, Cayman Chemicals, stock made in water) is added first ...
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bioRxiv - Neuroscience 2023Quote: HDAC activity of class I HDACs (HDAC1, 2, 3 and 8) was measured with the fluorometric HDAC Activity Assay kit: HDAC1 (10011563, Cayman Chemical, Ann Arbor, MI), HDAC2 ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h before being incubated overnight at 4°C with primary antibodies directed against the N-terminal extracellular region of the CB1R (1:500, rabbit polyclonal antibody, Cayman Chemical Company, Cat# 101500, Lot# 0580081-1) and βactin (1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was supplemented on the different days as follows: Day 0 to Day 2, 10ng/ml bFGF (R&D, Cat. No. 100-18B) and 5 µM LGK (Cayman Chemical Company, Cat. No. 1.800.364.9897); Day 2 to Day 3 for 20 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1-[2-chloro-6-[[(3-iodophenyl)methyl]amino]-9H-purin-9-yl]-1-deoxy-N-methyl-β-D-ribofuranuronamide (2-Chloro-IB-MECA; Cayman Chemicals, 163042-96-4), [2-Amino-4-[3-(trifluoromethyl)phenyl]-3-thienyl] phenylmethanone (VCP 171 ...