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Citations for Cayman Chemical :
901 - 950 of 979 citations for Carbamic acid 2 4 hexadienyl 1 1 dimethylethyl ester E E 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... washed with PBS 1X twice and incubated with 100 μM of 2-NBDG (Cayman Chemical) for 30 minutes at 37°C in a 5% CO2 humid atmosphere ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μM 5-ethynyl-2’-deoxyuridine (EdU, Cayman Chemical Cat#20518, CAS# 61135-33-9). For drug treatments and time courses ...
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bioRxiv - Neuroscience 2021Quote: Plasma samples were diluted to 1:30 and assayed using a testosterone ELISA according to the manufacturer’s instructions (#582701, Cayman Chemical, Ann Arbor, MI, USA). For measurements of testicular testosterone levels ...
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bioRxiv - Neuroscience 2020Quote: ... Oxytocin concentrations in 1-ml plasma samples were measured in triplicate using a commercial ELISA assay (Cayman Chemical, Ann Arbor, Michigan USA Kit 500440) and a prior extraction step was performed in accordance with the manufacturers recommended protocol ...
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bioRxiv - Microbiology 2021Quote: ... The fixed cells were pelleted by centrifugation for 1 min at 4000 rpm then resuspended in PBS with 10 μg/mL Hoechst 33342 (Cayman Chemical; Item no. 15542). Aliquots of stained cells were loaded onto glass microscope slides and visualized using an Olympus IX70 Epifluorescent Microscope fitted with a Hamamatsu C11440 camera.
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bioRxiv - Bioengineering 2022Quote: ... 1mL of the AggreWell medium was manually replaced with supplemented medium containing WNT inhibitor (IWR1-ε, 3 µM, Cayman Chemical, 13659, days 1-10) and Nodal/Activin inhibitor (SB431542 ...
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bioRxiv - Microbiology 2020Quote: ... After 24 to 48 hours of incubation at 30 °C (depending on the growth speed of target strain) 1 ml of the dye resazurin (Cayman Chemical Company, Michigan, USA) was added to each well at a concentration of 50 mg L−1 and incubated for half an hour before the surplus was removed ...
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bioRxiv - Cancer Biology 2022Quote: ... Trp53 and Smad4 mutations were selected by removing L-WRN conditioned media and adding 10 µm Nutlin-3 (an Mdm2 inhibitor enabling functional selection for Trp53 loss of function) (Cayman Chemical Company #10004372-1). 10 subclones were picked from the engineered bulk organoids ...
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bioRxiv - Synthetic Biology 2022Quote: Cell lysis was evaluated by measuring the optical density at 600 nm (OD600) of cell cultures after either the addition of N-3-oxo-dodecanoyl-L-homoserine lactone (AI-1, Cayman Chemicals, Ann Arbor, MI), hydrogen peroxide ...
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bioRxiv - Molecular Biology 2022Quote: ... IMR-32 or U118-MG cells were plated in 96 well plates and treated with BMAA and/or 1 uM XAV939 (Cayman Chemicals Item No. 13596) after 24 hours of incubation at normal conditions.
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bioRxiv - Immunology 2023Quote: ... Filtered serum was diluted 1:8 in assay buffer and measured for total nitrate and nitrite using Nitrate/Nitrite Colorimetric Assay Kit (Cayman Chemical Cat. No. 780001). Absorbance was measured at 540 nm using a BioTek Synergy H1 Microplate Reader ...
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bioRxiv - Immunology 2023Quote: ... violaceum colony was grown overnight in BHI broth then diluted to 1×106 CFU/mL of PBS and mixed with various concentrations of DEA NONOate (Cayman Chemical Cat. No. 82100) with or without itaconic acid (Sigma-Aldrich Cat ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: Phalloidin staining at E13.5 coronal sections was performed by incubating 1X Phalloidin working solution (1X PBS, 10% BSA and Phalloidin-iFluor™ 488) 1000x conjugate in DMSO (1:1000; Cayman Chemical Company 20549) for 45 minutes ...
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bioRxiv - Genomics 2020Quote: ... and addition of serum-free DMEM containing 4 μg/mL DyLight549-conjugated human LDL (Cayman Chemical, Ann Arbor MI, 10011229). Cells were incubated for 1 hr at 37°C then harvested with trypLE express ...
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bioRxiv - Cancer Biology 2020Quote: ... for 5 hours or treated with 10 µM of buthionine sulfoximine for 24 hours (BSO, Cayman Chemicals, 83730-53-4) and fixed with 4% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Microbiology 2022Quote: Cell lysates were harvested 4 days post reactivation in RIPA and subjected to enzyme-linked immunosorbent assay (ELISA) analysis for 2’3’-cGAMP (Cayman Chemical) according to the manufacturers’ protocols ...
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bioRxiv - Immunology 2022Quote: ... cGAMP was measured in undiluted BALF using the 2’,3’-Cyclic GAMP ELISA Kit (Cayman Chemical) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells recovered for 2 days in full media before the addition of 100nM LGK974 (Cayman Chemicals). The polyclonal cell lines were validated for editing using TIDE (tracking of insertions and deletions ...
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bioRxiv - Genomics 2022Quote: ... 5-aza-2’-deoxycytidine (5Aza-dC) was purchased from Cayman Chemical (Item No. 11166, Purity > 98%), TDCIPP was purchased from MedChemExpress (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 7.2-7.4) supplemented with 10 mg/ml cytochalasin B (Cayman Chemical Company, 14930-96-2), 2000 nM MitoTracker Green FM (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were pretreated for 2 h with 200 nM bafilomycin A1 (BafA1; Cayman Chemical, 11038). The same volume of DMSO (MP Biomedicals ...
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bioRxiv - Cancer Biology 2024Quote: ... Olaparib was dissolved in DMSO and then diluted in 10% 2-hydroxypropyl-β-cyclodextrin (Cayman Chemicals), and AZD9574 was dissolved in 1M methanesulfonic acid (MSA ...
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bioRxiv - Cell Biology 2020Quote: ... and tSETD2-Flag (a.a. 1418-2564) was measured over 3-4 hours using a Methyltransferase Fluorescence Assay Kit (Cayman Chemical, 700150), a continuous enzyme-coupled assay that continuously monitors SAM-dependent methyltransferase activity27,28 ...
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bioRxiv - Molecular Biology 2021Quote: Mice kept in doxycycline-containing chow were treated for 5 days with 4% w/v DSS (FW 40.000) (Cayman Chemical #23250) dissolved in drinking water ...
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bioRxiv - Microbiology 2021Quote: The assessment of astodrimer sodium cytotoxicity occurred on Day 4 by measuring lactate dehydrogenase (LDH) activity in the cytoplasm using an LDH detection kit (Cayman Chemical), with 0.5% saponin used as the positive cytotoxic control.
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bioRxiv - Molecular Biology 2022Quote: ... cells in selected wells were first washed with pre-warmed serum-free DMEM and then incubated in serum free DMEM containing 4 μg/mL fluorescently DyLight550-conjugated LDL (Cayman Chemical) at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... and microglia were exposed to increasing concentrations of CDDO-Me (CAS number 218600-53-4, Cayman Chemical, Ann Arbor, MI, USA) at the timepoints specified in the figure legends ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 U/ml penicillin and 0.1 mg/ml streptomycin) supplemented with 2 mM D-luciferin (Cayman Chemicals) and 10 μM 9-cis-retinal in experiments with light-activated receptors ...
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bioRxiv - Immunology 2020Quote: ... sodium nitrite and Polyclonal antibody against mouse COX-2 (Item no. 160126) were obtained from Cayman Chemicals, USA ...
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bioRxiv - Neuroscience 2020Quote: ... The following drugs were used: Y-27632 (50 μM, Cayman Chemical, #10005583-5, CAS: 129830-38-2), blebbistatin (100 μM ...
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bioRxiv - Biochemistry 2022Quote: ... Nitrite concentrations were determined by reacting 100 μL aliquots of reaction sample with 50 μL of deoxygenated Griess reagent R1 (Cayman Chemical, 1% sulfanilamide in 5% H3PO4) followed by addition of 50 μL of deoxygenated Griess reagent R2 (Cayman Chemical ...
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bioRxiv - Bioengineering 2024Quote: ... cells were allowed to rest for 5 minutes then added to pre-warmed wells of a 24 well plate containing media (as specified in CD34+ cell culture) supplemented with small molecules as indicated for specific experiments (AZD7648, RS-1, Cayman Chemicals; M-3814, Toronto Research Chemicals). Where AAV donor was used ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were centrifuged at 10,000 xg for 10 minutes at 4°C and the supernatant was processed (undiluted) according to manufacturer instructions at room temperature (Cayman Chemical, 10010303).
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were centrifuged at 10,000 xg for 10 minutes at 4°C and the supernatant was processed (undiluted) according to manufacturer instructions at room temperature (Cayman Chemical, 10010303). Mouse placenta samples (stored at -80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... A metabolic labeling protocol was followed[6] and the experimental HEK293 cells were incubated in the presence of 40µM unphosphorylated 4-thiouridine (Cayman Chemical, CAY16373-5). Total RNA was harvested from the cells using Trizol (Ambion ...
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bioRxiv - Biophysics 2022Quote: ... Modifications in DNA ultrastructure were done to condense or decondense chromatin with the use of 600 nM trichostatin A (TSA) for 4 h (Cayman Chemical Company), to increase euchromatin ...
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bioRxiv - Physiology 2024Quote: ... Plasma was isolated from the terminal blood collections via centrifugation (3000rpm for 15 minutes at 4°C) and was run in duplicate on a competitive corticosterone ELISA (Cayman Chemicals #501320) after a single freeze-thaw ...
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bioRxiv - Genomics 2023Quote: ... Quantitation was done by the external standards calibration of DPPC and PC 16:0/18:2 (Cayman Chemicals) where instrument response for the analyte was measured as a ratio between analytes and internal standard’s peak areas.
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bioRxiv - Molecular Biology 2022Quote: ... Calcein-AM was purchased from Thermo (Waltham, MA, USA; Cat: C1430) and 1-Ethoxycarbonylmethyl-6-methoxyquinolinium bromide (MQAE) was purchased from Cayman Chemical (Ann Arbor, MI, USA; Cat:19585). Pluronic acid (Pluronic F-127 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained with β-Galactosidase Staining Solution containing 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal: #16495, Cayman Chemical) for 13 h at 37□ ...
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bioRxiv - Cancer Biology 2020Quote: ... The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was performed according to the manufacturer’s recommendation (10009365, Cayman Chemicals). After incubation with the MTT reagent ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-231 or BT-549 cells were treated with 2 μM NVP-BKM120 (Cayman Chemical, CAS #944396-07-0), 5 μM of Afuresertib (GSK2110183 ...
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bioRxiv - Biochemistry 2022Quote: ... and [1H,15N]-TROSY-HSQC of the LBD with or without 2 molar equivalents of rosiglitazone (Cayman Chemical #71740). 1D [1H]-NMR spectra were collected using natural abundance proteins that were not isotopically labeled ...
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bioRxiv - Immunology 2023Quote: ... 20 µL 10 mM N-[6-(biotinamido)hexyl]-3′-(2′-pyridyl dithio)propionamide (biotin-HPDP, Cayman Chemical Company, 16459) in DMSO was added to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was performed according to the manufactureŕs recommendation (10009365; Cayman Chemical). After incubation with the MTT reagent ...
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bioRxiv - Cancer Biology 2022Quote: ... Arp 2/3 complex inhibitor (CK-666≤200μM) and CDC42 inhibitor (ML-141≤20μM) were obtained from Cayman Chemical. The PI3K pathway inhibitor (LY294002≤100μM) ...
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bioRxiv - Microbiology 2023Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) assay was performed using an MTT assay kit (Cayman Chemical). Cells in 96-well plates were treated with DMSO or desired chemical inhibitors at different concentrations and incubated for desired time ...
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bioRxiv - Cell Biology 2024Quote: ... Mice received a single intraperitoneal injection of 5-ethynyl-2’-deoxyuridine (EdU, 10mg/kg, Cayman Chemical, Ann Arbor, MI) immediately after MI surgery and continued daily through post-op Day 14.
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bioRxiv - Microbiology 2024Quote: ... The following reagents were added at the indicated concentrations unless otherwise noted: 2-deoxyglucose [10 mM (Cayman Chemical Company)] ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were treated with Erastin(10 μM) for 6 or 18 hours or RSL3(2 μM)(Cayman Chemicals) for 2 hours in the presence and absence of Liproxstatin (1 μM) ...