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Citations for Cayman Chemical :
801 - 850 of 871 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... 1mL of the AggreWell medium was manually replaced with supplemented medium containing WNT inhibitor (IWR1-ε, 3 µM, Cayman Chemical, 13659, days 1-10) and Nodal/Activin inhibitor (SB431542 ...
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bioRxiv - Synthetic Biology 2022Quote: Cell lysis was evaluated by measuring the optical density at 600 nm (OD600) of cell cultures after either the addition of N-3-oxo-dodecanoyl-L-homoserine lactone (AI-1, Cayman Chemicals, Ann Arbor, MI), hydrogen peroxide ...
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bioRxiv - Cancer Biology 2022Quote: ... Trp53 and Smad4 mutations were selected by removing L-WRN conditioned media and adding 10 µm Nutlin-3 (an Mdm2 inhibitor enabling functional selection for Trp53 loss of function) (Cayman Chemical Company #10004372-1). 10 subclones were picked from the engineered bulk organoids ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 U/ml penicillin and 0.1 mg/ml streptomycin) supplemented with 2 mM D-luciferin (Cayman Chemicals) and 10 μM 9-cis-retinal in experiments with light-activated receptors ...
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bioRxiv - Immunology 2020Quote: ... sodium nitrite and Polyclonal antibody against mouse COX-2 (Item no. 160126) were obtained from Cayman Chemicals, USA ...
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bioRxiv - Pathology 2020Quote: ... SCH79797 (PAR-1 antagonist) and GB-83 (PAR-2 antagonist) were purchased from Cayman Chemicals (Michigan, USA). U0126 (MEK 1/2 inhibitor) ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were centrifuged at 10,000 xg for 10 minutes at 4°C and the supernatant was processed (undiluted) according to manufacturer instructions at room temperature (Cayman Chemical, 10010303).
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were centrifuged at 10,000 xg for 10 minutes at 4°C and the supernatant was processed (undiluted) according to manufacturer instructions at room temperature (Cayman Chemical, 10010303). Mouse placenta samples (stored at -80°C ...
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bioRxiv - Biophysics 2022Quote: ... Modifications in DNA ultrastructure were done to condense or decondense chromatin with the use of 600 nM trichostatin A (TSA) for 4 h (Cayman Chemical Company), to increase euchromatin ...
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bioRxiv - Physiology 2024Quote: ... Plasma was isolated from the terminal blood collections via centrifugation (3000rpm for 15 minutes at 4°C) and was run in duplicate on a competitive corticosterone ELISA (Cayman Chemicals #501320) after a single freeze-thaw ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSCs were passed and replated to be maintained in E8 medium with 5 μM of Y-27632 (Cayman Chemical, USA) with daily changes of media ...
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bioRxiv - Neuroscience 2022Quote: ... Offspring were then randomly assigned to receive daily injections (i.p.) of THC (1:1:18 THC 5 mg/kg [Cayman Chemicals] ...
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bioRxiv - Immunology 2022Quote: ... 100 μM apocynin was used to block ROS generation via NOX2 complex by PMA and 5 μM mitoTEMPO (Cayman Chemical) was used to inhibit mitoROS production ...
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bioRxiv - Immunology 2023Quote: ... Samples were prepared for HPLC injection by mixing 5 µL of lipid extract with 2.5 µL of an internal standard mixture (all from Cayman Chemicals) consisting of 2.5 ng LPC(O-16:0- D4/0:0) ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: ... then blocked for 60 minutes in PBST containing 5% normal goat serum (NGS) (Cayman Chemical, Cat#10006577, Ann Arbor, MA). Solution was then switched to 5% NGS/PBST containing primary antibodies (see below ...
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bioRxiv - Molecular Biology 2023Quote: The remaining compounds were sourced commercially without additional purification: necrostatin 1 and necrostatin 5 (Cayman Chemical, Ann Arbor, MI, USA), necrostatin 1S (MedChemExpress ...
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bioRxiv - Microbiology 2022Quote: The effects of NDM-1 and NMC-A carbapenemases on 5 mg/ml of imipenem (imipenem hydrate ≥98%, Cayman Chemicals) solution containing 16 mM NaHCO3 and 0.15 mM ZnSO4 as well as on 5 mg/ml imipenem – 5 mg/ml cilastatin in 0.15 mM ZnSO4 prepared from the brand name Primaxin formulation (Merck ...
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bioRxiv - Immunology 2024Quote: ... and either 166.6 μM BSA-conjugated palmitate (stock of 5 mM palmitate:0.8 mM BSA at a 6:1 molar ratio; cat. no. 29558, Cayman Chemical) or 27.8 µM BSA control (cat ...
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bioRxiv - Cancer Biology 2020Quote: ... Aphidicolin (working concentration 0,4 μM) and AZ3146 (working concentration 1 or 2 μM) were purchased from Cayman Chemical; SB203580 (working concentration 10 μM ...
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bioRxiv - Genomics 2023Quote: ... Quantitation was done by the external standards calibration of DPPC and PC 16:0/18:2 (Cayman Chemicals) where instrument response for the analyte was measured as a ratio between analytes and internal standard’s peak areas.
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bioRxiv - Plant Biology 2020Quote: Metazachlor treatment was performed on seedlings grown for 5 days on half MS plates containing 50 or 100 nM metazachlor (Cayman Chemical). Metazachlor was added to the medium from a 100 mM stock in dimethylsulfoxide by using an intermediate diluted stock at 100 μM (extemporarily prepared).
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bioRxiv - Neuroscience 2022Quote: ... lysates were diluted 1:5 (cingulate cortex, cerebellum, putamen) or 1:10 (frontal cortex) into assay buffer containing 0.25% sodium taurocholate (Cayman Chemical, 16215), 0.25% triton-X-100 (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Plates were blocked with 5% low-fat milk in PBS and antigens citrullinated with 280mU/ml recombinant human PAD4 (Cayman chemicals) in 50 mM Tris 10 mM CaCl2 1 mM DTT for 3h at 37C ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were seeded into 12-well plates or chamber slides at a density of 1-5 million cells/mL and treated with vehicle (DMSO) or 5nM Docetaxel (Cayman Chemical) in DMSO for 72h ...
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bioRxiv - Microbiology 2024Quote: ... was obtained from Abcam and used at a concentration of 5 μM. 7-Keto Cholesterol (7-KC) (Cat. No. 16339) was obtained from Cayman Chemical Company and was used at 30 μM ...
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bioRxiv - Cell Biology 2024Quote: ... human colonoids were cultured in IFE medium supplemented with 10 µM Y-27632 and 5 µM Prostaglandin E2 (PGE2) (CAY14010, Cayman Chemical) 76 for 3-5 days after splitting ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-231 or BT-549 cells were treated with 2 μM NVP-BKM120 (Cayman Chemical, CAS #944396-07-0), 5 μM of Afuresertib (GSK2110183 ...
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bioRxiv - Biochemistry 2022Quote: ... and [1H,15N]-TROSY-HSQC of the LBD with or without 2 molar equivalents of rosiglitazone (Cayman Chemical #71740). 1D [1H]-NMR spectra were collected using natural abundance proteins that were not isotopically labeled ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... we performed the reaction under identical conditions using 1-NBD-decanoyl-2-decanoyl-sn-Gly (NBD-DAG) (Cayman Chemical) as the substrate ...
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bioRxiv - Microbiology 2024Quote: ... The following reagents were added at the indicated concentrations unless otherwise noted: 2-deoxyglucose [10 mM (Cayman Chemical Company)] ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were treated with Erastin(10 μM) for 6 or 18 hours or RSL3(2 μM)(Cayman Chemicals) for 2 hours in the presence and absence of Liproxstatin (1 μM) ...
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bioRxiv - Biochemistry 2024Quote: ... Crystals of the dPCoA complex were obtained by adding 0.8 µL 6.7 mg mL-1 CarI TAD Δhelix protein containing 2 mM dPCoA (Cayman Chemical) to 0.4 µL 100 mM bis-tris propane pH 8.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... age and gender-matched 10-16 week old Tal1-creERT2 control mice and Dnmt3a R878H Tal1-creERT2 mice were treated with tamoxifen (4 mg/kg/day; Cayman Chemical, Ann Arbor, Michigan) for 2 doses ...
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bioRxiv - Neuroscience 2024Quote: ... and GALC enzyme assays using the respective 4-Methylumbelliferyl (4MU)-conjugated fluorometric substrates [PPT1 – 4MU-6-thio-palmitate-β-D-glucopyranoside (Cayman Chemical Co, #19524), GALC – 6HMU-β-D-galactoside (Carbosynth ...
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bioRxiv - Neuroscience 2022Quote: We validated our physiologically compatible THC solution (1:1:18 THC:cremophor:saline at 2.5, 5, or 10 mg/kg of THC; Cayman Chemicals, Ann Arbor, MI, USA) by measuring plasma levels of THC and two THC metabolites (11-hydroxy-delta-9-THC and 11nor-9carboxy delta-9-THC ...
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bioRxiv - Biochemistry 2023Quote: Purified human PPP1R15A single repeat peptides (UK2997, UK3098) were labelled with 10-fold molar concentration of fluorescein-5-malamide (16383, Cayman Chemical Company) in the presence of 1 mM TCEP at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: Glioblastoma samples were prepared with the following matrix application protocol: 100 µL of a 5 mg/mL deuterated tryptophan (D5-Trp) solution (Cayman Chemicals, 34829) in ACN/H2O (50:50 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then transferred to a fresh 96 well plate and diluted to 1.0 × 106 cells per ml in hTCM+IL-2 and 0.05 µM of the small molecule inhibitor TSA (Cayman Chemical). The 96 well plate was then spun at 200xg for 7 min and incubated in a tissue culture incubator overnight ...
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bioRxiv - Physiology 2024Quote: ... The uptake of fluorescent 2-NBDG was assessed according to manufacturer’s instructions (Cayman Chemicals - Glucose Uptake Cell-Based Assay Kit) on a Synergy HTX plate reader (BioTek).
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bioRxiv - Developmental Biology 2022Quote: ... cohorts were pre-treated for 2 hours in mTOR inhibitor (Torin, final concentration 100 nM in fish water, Cayman Chemical). For experiments using rapamycin (LC Labs ...
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bioRxiv - Immunology 2024Quote: ... while the remaining cells were subjected to the same regimen with 2 µM vemurafenib and an additional 1.5 nM cobimetinib (GDC-0973, Cayman Chemical) until a final concentration of 200 nM cobimetinib was attained ...
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bioRxiv - Biochemistry 2024Quote: ... and 15 μL of 10X SARS-CoV-2 Main Protease (recombinant) solution (Cayman Chemical, Item No. 701963, Batch 0673677-1) were added in triplicate ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with the following primary antibodies prepared in antibody diluent (TBS solution with 0.02% Triton X-100 plus 1% bovine serum albumin and 2% normal goat serum): mouse anti-adropin (Cat. No. 14117, 1:200; Cayman Chemical), rat anti-CD31 (Cat ...
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bioRxiv - Pathology 2021Quote: ... cells media was changed to DMEM containing 2% charcoal striped and dialyzed media with 0.1 uM of T0901317 and/or 1uM of LG100268 (noted LG268) (Cayman Chemical, USA). After overnight treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... inhibitors were pre-incubated with MB solution 2 h before irradiation: Fer-1 (Cayman Chemical, Ann Arbor, Michigan, EUA, 1 µM); Nec-1 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and seeded at a density of 500,000 cells per well of a matrigel-coated 6-well plate in iPS-Brew XF supplemented with 2 uM Thiazovivin ROCK inhibitor (Cayman Chemical). Definitive endoderm was derived using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed in PBS and resuspended at 1×106 cells/mL in fresh media and pre-treated for 2 hours with 0.5mM Glutathione ethyl ester (Et-GSH, Cayman Chemical, 14953) or 50nM UAMC-3203 (Cayman Chemical ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Leukotriene D4 (LTD4, Cat# 20310) and 2-Methylthioadenosine diphosphate (2MeS-ADP, Cat# 21230) were purchased from Cayman Chemical (Ann Arbor, Michigan). HAMI 3379 was purchased from MedChemExpress (Monmouth Junction ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were trypsinized and plated at 40% confluence on fibronectin-coated cover glass in regular GM containing 1μM ERK1/2 inhibitor (Cayman Chemicals; SCH772984). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... Suspensions were centrifuge at 3,000 g for 5 min and the cAMP levels in supernatants were measured using the cAMP EIA Kit (Cayman Chemical, Ann Arbor, MI, US) according to the manufacturer’s instructions ...