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Citations for Cayman Chemical :
651 - 699 of 699 citations for 2' 5' Dichloro 3 3 4 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and [1H,15N]-TROSY-HSQC of the LBD with or without 2 molar equivalents of rosiglitazone (Cayman Chemical #71740). 1D [1H]-NMR spectra were collected using natural abundance proteins that were not isotopically labeled ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... we performed the reaction under identical conditions using 1-NBD-decanoyl-2-decanoyl-sn-Gly (NBD-DAG) (Cayman Chemical) as the substrate ...
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bioRxiv - Microbiology 2024Quote: ... The following reagents were added at the indicated concentrations unless otherwise noted: 2-deoxyglucose [10 mM (Cayman Chemical Company)] ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were treated with Erastin(10 μM) for 6 or 18 hours or RSL3(2 μM)(Cayman Chemicals) for 2 hours in the presence and absence of Liproxstatin (1 μM) ...
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bioRxiv - Biochemistry 2024Quote: ... Crystals of the dPCoA complex were obtained by adding 0.8 µL 6.7 mg mL-1 CarI TAD Δhelix protein containing 2 mM dPCoA (Cayman Chemical) to 0.4 µL 100 mM bis-tris propane pH 8.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... age and gender-matched 10-16 week old Tal1-creERT2 control mice and Dnmt3a R878H Tal1-creERT2 mice were treated with tamoxifen (4 mg/kg/day; Cayman Chemical, Ann Arbor, Michigan) for 2 doses ...
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bioRxiv - Neuroscience 2024Quote: ... and GALC enzyme assays using the respective 4-Methylumbelliferyl (4MU)-conjugated fluorometric substrates [PPT1 – 4MU-6-thio-palmitate-β-D-glucopyranoside (Cayman Chemical Co, #19524), GALC – 6HMU-β-D-galactoside (Carbosynth ...
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bioRxiv - Neuroscience 2022Quote: We validated our physiologically compatible THC solution (1:1:18 THC:cremophor:saline at 2.5, 5, or 10 mg/kg of THC; Cayman Chemicals, Ann Arbor, MI, USA) by measuring plasma levels of THC and two THC metabolites (11-hydroxy-delta-9-THC and 11nor-9carboxy delta-9-THC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The experiment was stopped by the addition of 5% trichloroacetic acid (TCA) and the concentration of cGMP was measured using the cGMP enzyme immuno assay (EIA) kit (Cayman Chemical Company).
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bioRxiv - Biochemistry 2023Quote: ... cell lysates were incubated for 5 min with competitor (Arachidonic acid, docosanoic acid, Cayman Chemical 9000338, or 8-HETE, Cayman Chemical 34340) at 37°C before addition of other substrates and initiation with NADPH.
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bioRxiv - Biochemistry 2023Quote: Purified human PPP1R15A single repeat peptides (UK2997, UK3098) were labelled with 10-fold molar concentration of fluorescein-5-malamide (16383, Cayman Chemical Company) in the presence of 1 mM TCEP at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: Glioblastoma samples were prepared with the following matrix application protocol: 100 µL of a 5 mg/mL deuterated tryptophan (D5-Trp) solution (Cayman Chemicals, 34829) in ACN/H2O (50:50 ...
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bioRxiv - Cancer Biology 2022Quote: ... using a glutathione assay kit based on Ellman’s reagent (DTNB, 5,5′-dithio-bis-2-nitrobenzoic acid) according to the manufacturer’s protocol (Cayman Chemical, t#703002). Data are presented as DNTB fluorescence normalized to total protein ...
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bioRxiv - Developmental Biology 2022Quote: ... cohorts were pre-treated for 2 hours in mTOR inhibitor (Torin, final concentration 100 nM in fish water, Cayman Chemical). For experiments using rapamycin (LC Labs ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then transferred to a fresh 96 well plate and diluted to 1.0 × 106 cells per ml in hTCM+IL-2 and 0.05 µM of the small molecule inhibitor TSA (Cayman Chemical). The 96 well plate was then spun at 200xg for 7 min and incubated in a tissue culture incubator overnight ...
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bioRxiv - Physiology 2024Quote: ... The uptake of fluorescent 2-NBDG was assessed according to manufacturer’s instructions (Cayman Chemicals - Glucose Uptake Cell-Based Assay Kit) on a Synergy HTX plate reader (BioTek).
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bioRxiv - Immunology 2024Quote: ... while the remaining cells were subjected to the same regimen with 2 µM vemurafenib and an additional 1.5 nM cobimetinib (GDC-0973, Cayman Chemical) until a final concentration of 200 nM cobimetinib was attained ...
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bioRxiv - Biochemistry 2024Quote: ... and 15 μL of 10X SARS-CoV-2 Main Protease (recombinant) solution (Cayman Chemical, Item No. 701963, Batch 0673677-1) were added in triplicate ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with the following primary antibodies prepared in antibody diluent (TBS solution with 0.02% Triton X-100 plus 1% bovine serum albumin and 2% normal goat serum): mouse anti-adropin (Cat. No. 14117, 1:200; Cayman Chemical), rat anti-CD31 (Cat ...
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bioRxiv - Pathology 2021Quote: ... cells media was changed to DMEM containing 2% charcoal striped and dialyzed media with 0.1 uM of T0901317 and/or 1uM of LG100268 (noted LG268) (Cayman Chemical, USA). After overnight treatment ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with DMEM containing 2% FBS and labelled with 30 μM 17-ODYA (17-Octadecynoic Acid,34450, Cayman Chemicals) or DMSO for 3hr at 37°C incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... inhibitors were pre-incubated with MB solution 2 h before irradiation: Fer-1 (Cayman Chemical, Ann Arbor, Michigan, EUA, 1 µM); Nec-1 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and seeded at a density of 500,000 cells per well of a matrigel-coated 6-well plate in iPS-Brew XF supplemented with 2 uM Thiazovivin ROCK inhibitor (Cayman Chemical). Definitive endoderm was derived using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed in PBS and resuspended at 1×106 cells/mL in fresh media and pre-treated for 2 hours with 0.5mM Glutathione ethyl ester (Et-GSH, Cayman Chemical, 14953) or 50nM UAMC-3203 (Cayman Chemical ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Leukotriene D4 (LTD4, Cat# 20310) and 2-Methylthioadenosine diphosphate (2MeS-ADP, Cat# 21230) were purchased from Cayman Chemical (Ann Arbor, Michigan). HAMI 3379 was purchased from MedChemExpress (Monmouth Junction ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were trypsinized and plated at 40% confluence on fibronectin-coated cover glass in regular GM containing 1μM ERK1/2 inhibitor (Cayman Chemicals; SCH772984). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... Suspensions were centrifuge at 3,000 g for 5 min and the cAMP levels in supernatants were measured using the cAMP EIA Kit (Cayman Chemical, Ann Arbor, MI, US) according to the manufacturer’s instructions ...
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Ventral tegmental area glutamate neurons mediate the nonassociative consequences of traumatic stressbioRxiv - Neuroscience 2021Quote: ... Baseline firing in response to current injections was collected for 5 min followed by a 10 min bath application of 10 μM clozapine (Cayman Chemical, Ann Arbor, MI). The average number of action potentials generated during five consecutive sweeps was used to quantify baseline activity and the aver-age of five consecutive sweeps during clozapine application was used to quantify firing during hM4Di receptor activation ...
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bioRxiv - Biophysics 2022Quote: ... were induced along the PM of cultured HeLa cells by adding 10-15% ethanol (25) or 5 µM cytochalasin D (Cayman Chemical, Ann Arbor, MI) to the cell growth medium and incubating for ∼15 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were washed for 3 h and incubated for 30 min (37 °C, 5% CO2) with either 25 µM spermine-NONOate (a nitric oxide donor; Cayman Chemical, cat. no. 82150) or 25 µM sulpho-NONOate (control compound ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were then injected 30 minutes prior to behavioral experiments intraperitoneally with 5 mg/kg of the iDREADD agonist clozapine-n-oxide (CNO; Cayman Chemical, #34233-69-7) and returned to their homecage until behavioral testing ...
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bioRxiv - Developmental Biology 2022Quote: ... (gift from Jeremy Nathans (41)) or C3H10T1/2-STF (ATCC CCL-226) cells were treated with 1 µM LGK-974 (Cayman Chemical 14072) for 48 h to inhibit endogenous WNT secretion ...
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bioRxiv - Microbiology 2020Quote: Neutralizing assay was performed using SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA; Item No. 502070) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... were immobilized in a PDMS microfluidic olfactory chip [58,59] and exposed to either a control buffer or a stimulus buffer containing 100mM 2-AG (Cayman Chemical Co. #62160). The required volume of 2-AG required to make 100mM solution was dissolved in 0.1% ethanol before being added to S-Basal buffer ...
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bioRxiv - Physiology 2023Quote: ... The reaction proceeded for 2 hours at 37°C in presence of either 300 nM MS-275 (Cayman Chemical, Ann Arbor, MI) or vehicle (0.01% DMSO) ...
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bioRxiv - Developmental Biology 2024Quote: ... were cultured in 2 ml retina culture medium in a 24-well plate with or without 10 µM retinoic acid (Cayman Chemical 11017) and incubated at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and BMS493 was further diluted to a concentration of 2 mg/mL in DPBS supplemented with 0.15M hydroxypropyl β-cyclodextrin (HP-β-CD; Cayman Chemicals 16169), for a total volume of 0.5 mL per dose (1 mg/dose) ...
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bioRxiv - Biochemistry 2022Quote: ... females were anaesthetized and injected (2×69nl) with tyrosine kinase inhibitors (all 200μM) SU6656 (CAS 330161-87-0, Cayman Chemical, Ann Arbor, USA), genistein (CAS 446-72-0 ...
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bioRxiv - Microbiology 2022Quote: To estimate pulmonary oxidative stress we measured oxidized guanosine (8-hydroxyguanosine, 8-hydroxy-2’-deoxyguanosine, and 8-hydroxyguanine) in the lungs of mice using an ELISA (Cayman Chemical cat. 589320) following the manufacturer’s instructions and analysis template.
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bioRxiv - Neuroscience 2024Quote: ... containing the deuterated internal standards 2-arachidonoylglycerol-d5 (100 ng/ml) and arachidonoylethanolamide-d (1 ng/ml) (Cayman Chemical, Ann Arbor, MI, USA). Samples were homogenized and centrifuged on an Eppendorf miniSpin microtube centrifuge at 13,400 rpm for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... tubacin (10 and 20µM; Selleckchem, Houston, TX), Nintedanib (24h 2 µM; Selleckchem, Houston, TX) and PGE2 (96hr, 1 μM, Cayman Chemicals, Ann Arbor, MI) or FGF2 (96 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Lipid mediator formation was stopped with ice-cold methanol (2 mL for PBMCs and 3.5 mL for macrophages) containing deuterium-labeled internal standards (Cayman Chemical, Ann Arbor, MI), i.e. ...
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bioRxiv - Biochemistry 2022Quote: ... Nitrite concentrations were determined by reacting 100 μL aliquots of reaction sample with 50 μL of deoxygenated Griess reagent R1 (Cayman Chemical, 1% sulfanilamide in 5% H3PO4) followed by addition of 50 μL of deoxygenated Griess reagent R2 (Cayman Chemical ...
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bioRxiv - Microbiology 2021Quote: Infected C57BL/6 WT and FPR2−/− mice were treated with 5 μg/kg/day aspirin-triggered Resolvin D1 (AT-RvD1) (Cayman Chemicals®, Ann Harbor, MI, USA), 30 mg/kg/day Bz (Abarax ‐ Elea Laboratory ...
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bioRxiv - Immunology 2022Quote: ... The membranes were washed three times at 5 minutes each with TBS-T and were incubated for 2-hours with a 1:10,000 dilution of an appropriate secondary antibody conjugated to horseradish peroxidase in 2% skim milk in TBS-T (Cayman Chemical Company, Ann Arbor, MI). Signal detection was achieved with a Super Signal West Pico Chemiluminescence kit (Pierce Biotechnology ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.18 mM KH2PO4) containing 1 mM CaCl2 or with 0.4 μg/ml BsClpP14 in the presence of 11 μM ADEP1 (Cayman Chemical, A-54556A, ~2-fold molar ratio)11 in the same PBS at 40 °C ...
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bioRxiv - Immunology 2022Quote: Skin samples stored in 700 µL Trizol were homogenized in a Precellys® 2 mL Soft Tissue Homogenizing Ceramic Beads Tube (Cayman Chemical, Ann Arbor, MI, USA) using the Precellys® 24 machine ...
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bioRxiv - Physiology 2023Quote: ... LD211 was used as a selective agonist for MC1-R (compound 28 in the original publication),23 [D-Trp8]-γ-MSH as a selective MC3-R agonist,24 THIQ as a selective MC4-R agonist (Cayman Chemical, USA, # 312637-48-2)25 and PG-901 as a selective MC5-R agonist.26 PG-20N was used as a selective MC5-R antagonist.27 LD211 ...
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bioRxiv - Microbiology 2024Quote: Lung lysates containing protease inhibitors were used to determine COX-2 activities using a COX fluorescent activity assay kit (Cayman Chemical, #700200, Ann Arbor, MI, USA). The assay utilizes the peroxidase component of COXs and the production of resorufin ...