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Citations for Cayman Chemical :
651 - 694 of 694 citations for 1 Hydroxymethyl benzo cd indol 2 1H one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Serum thromboxane B2 was measured to confirm COX-1 inhibition (39, 40) by ELISA per the manufacturer’s instructions (Cayman Chemical 19030). COX-2 specific enzymatic activity was measured using the cyclooxygenase activity assay kit (abcam ab204699 ...
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bioRxiv - Pathology 2022Quote: ... 20 µL of supernatant were taken and added to 175 µL of reaction buffer containing 1 µL of decanoyl p-nitroaniline fatty acid amide hydrolase substrate (Cayman Chemicals). The reaction was photocolorimetrically measured at 390 nm ...
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bioRxiv - Microbiology 2023Quote: ... Arachidonic acid (AA) (d11) and ceramide (Cer) d18:1-15:0 (d7) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Isopropanol (IPA) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 100 ng/ml spliceostatin A (kindly shared by Dr. Minoru Yoshida [RIKEN]) or 1 μM pladienolide B (Cayman Chemical) for 30 min or 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Animals were randomized into a vehicle or treatment groups once tumors were palpable (100–150 mm3) and treatment was initiated with either DMSO:Tween80:PBS (10%:10%:80%) or 20 mg kg−1 riociguat (Cayman Chemicals, 9000554) or cinaciguat (Cayman Chemicals ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were seeded into 12-well plates or chamber slides at a density of 1-5 million cells/mL and treated with vehicle (DMSO) or 5nM Docetaxel (Cayman Chemical) in DMSO for 72h ...
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bioRxiv - Cell Biology 2024Quote: ... Drug treatments used for pseudoislet studies included 40 μM 84-B10 (MedChem Express) for 48 h or 1 μM MKT077 (Cayman Chemical) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa or HEK293 wild-type cells were treated with 100 nM or 1 µM of Pladienolide B (PlaB, Cayman Chemical Company), 1 µM of Herboxidiene (HB ...
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bioRxiv - Neuroscience 2024Quote: ... modulators of the electron transport chain were injected at 1 μM concentration to the cells in a following order: oligomycin (#11342, Cayman Chemical), carbonyl cyanide-4 (trifluoromethoxy ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... were water-restricted and trained to press two levers on a fixed-ratio 1 schedule for oral fentanyl (fentanyl hydrochloride from Cayman Chemical Company ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed with 1X PBS and the media was changed to mTeSR+ supplemented with 1 µM CHIR-99021 (Cayman Chemical). After 24 hours (Day 0) ...
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bioRxiv - Physiology 2024Quote: ... and treated with or without 1 μg/mg insulin in the presence of 20 μM cytochalasin B (cat. no.11328, Cayman Chemical), a GLUT inhibitor for measuring non-specific glucose uptake ...
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bioRxiv - Cancer Biology 2024Quote: ... cis-11-eicosenoic acid/11(Z) eicosenoic acid (cis-11-C20:1, Sigma-Aldrich, Cat# 44878 or Cayman Chemical, Cat# 20606), 13(Z)-docosenoic acid (cis-13-C22:1 ...
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bioRxiv - Cell Biology 2022Quote: ... These cells were sub-cultured either in 6-well plates or T-25 flask and treated with 100nM (1:5000 µl of media) PMA (Phorbo 12-Myristate 13-Acetate, Cayman Chemicals, USA) to yield 60-70% adherent cells at the end of 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... for 15 min before incubation with the last diluted antibody: rabbit anti-V5-tag (GKPIPNPLLGLDST) conjugated with DyLight 550 at 1:300 dilution (Cayman Chemical, 11261) for another 24 h at room temperature.
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bioRxiv - Neuroscience 2023Quote: ... mice in the URB597 group were subjected to intraperitoneal injection with 1 mg/kg URB597 [25] (Cayman Chemicals, Ann Abor, MI, USA), and mice in the PE+AM630 group were subjected to intraperitoneal injection with 1 mg/kg AM630 [26] at 6 weeks before induction of the ischemia-reperfusion model (Fig ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The SOD activity of liver homogenate (containing 50 mM MES, pH 6.5, 1 mM EDTA) was determined using commercial SOD assay kit (Cayman Chemical Co., # 66970752). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were grown until confluency and differentiation of pre-adipocytes was induced by treatment with an adipogenic cocktail (1 µM rosiglitazone (Cayman Chemical 71740), 0.5 mM 3-Isobutyl-1-methylxanthine (IBMX ...
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: The same amount of RNP mentioned above was firstly premixed and then added to 1/10 volume of premixed SM102-LNP (Cayman Chemical, USA) according to the manufacturer’s instruction for the mRNA loading ...
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bioRxiv - Microbiology 2023Quote: ISE6 and IDE12 cells were seeded at 1 x 106 cells per well in a 24-well plate and pretreated with ISRIB (Cayman Chemical, 16258), or salubrinal (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 3-isobutyl-1-methylxantine (IBMX, Cat. No. 13347) and H-89 dihydrochloride (H-89, Cat. No. 10010556) were purchased from Cayman Chemical (USA). Dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... DOX (250 ng/ml) or AP-1 inhibitors (SR11302, Santa Cruz Biotechnology Cat #: sc-204295 at 10 uM concentration; T5224, Cayman Chemical, Cat # ...
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bioRxiv - Molecular Biology 2024Quote: ... the assay was assembled as follows: 1 μM PLpro in 50 mM HEPES buffer was incubated with increased concentration of compounds (Cayman chemicals, USA) ranging from 0.1 μM to 500 μM and was dispensed into wells of 96-well plate ...
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bioRxiv - Cell Biology 2022Quote: ... cells were grown in YES at 32°C to mid-log phase and treated with 4-amino-1-tert-butyl-3-(3-methylbenzyl)pyrazolo[3,4-3]pyrimidine (3MB-PP1) (Toronto Research Chemical; A602960 or Cayman Chemical; 17860) at a final concentration of 15 µM for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... aNSCs were cultured for 1 d and the medium was supplemented either with 10 μM GW4869 (Cayman Chemical Co., Ann Arbor, MI, USA) or dimethyl sulfoxide (DMSO ...
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bioRxiv - Genomics 2021Quote: ... at the appropriate dilution (typically 1:12 in PBS) for total cholesterol content using a commercial fluorometric assay (Cayman Chemical, Ann Arbor MI). Fluorescence outputs were measured on a Tecan SPARK plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... the media was changed into fresh HMC3 media containing fluorescent latex beads (1:500, latex -eads-rabbit IgG-FITC complex - Cayman chemical cat#500290). The cells were incubated for 30 min at 37°C before being washed as the manufacturer protocol ...
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bioRxiv - Developmental Biology 2023Quote: The FAAH inhibitor URB597 and the non-hydrolysable anandamide analog R-1 methanandamide (metAEA) were purchased from Cayman Chemical (Ann Arbor, MI, USA). The FAAH inhibitor BIA 10-2474 was purchased from Medchem Express (Monmouth Junction ...
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bioRxiv - Neuroscience 2021Quote: Plasma samples were diluted to 1:30 and assayed using a testosterone ELISA according to the manufacturer’s instructions (#582701, Cayman Chemical, Ann Arbor, MI, USA). For measurements of testicular testosterone levels ...
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bioRxiv - Neuroscience 2020Quote: ... Oxytocin concentrations in 1-ml plasma samples were measured in triplicate using a commercial ELISA assay (Cayman Chemical, Ann Arbor, Michigan USA Kit 500440) and a prior extraction step was performed in accordance with the manufacturers recommended protocol ...
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bioRxiv - Microbiology 2021Quote: ... The fixed cells were pelleted by centrifugation for 1 min at 4000 rpm then resuspended in PBS with 10 μg/mL Hoechst 33342 (Cayman Chemical; Item no. 15542). Aliquots of stained cells were loaded onto glass microscope slides and visualized using an Olympus IX70 Epifluorescent Microscope fitted with a Hamamatsu C11440 camera.
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bioRxiv - Bioengineering 2022Quote: ... 1mL of the AggreWell medium was manually replaced with supplemented medium containing WNT inhibitor (IWR1-ε, 3 µM, Cayman Chemical, 13659, days 1-10) and Nodal/Activin inhibitor (SB431542 ...
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bioRxiv - Microbiology 2020Quote: ... After 24 to 48 hours of incubation at 30 °C (depending on the growth speed of target strain) 1 ml of the dye resazurin (Cayman Chemical Company, Michigan, USA) was added to each well at a concentration of 50 mg L−1 and incubated for half an hour before the surplus was removed ...
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bioRxiv - Synthetic Biology 2022Quote: Cell lysis was evaluated by measuring the optical density at 600 nm (OD600) of cell cultures after either the addition of N-3-oxo-dodecanoyl-L-homoserine lactone (AI-1, Cayman Chemicals, Ann Arbor, MI), hydrogen peroxide ...
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bioRxiv - Molecular Biology 2022Quote: ... IMR-32 or U118-MG cells were plated in 96 well plates and treated with BMAA and/or 1 uM XAV939 (Cayman Chemicals Item No. 13596) after 24 hours of incubation at normal conditions.
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bioRxiv - Immunology 2023Quote: ... Filtered serum was diluted 1:8 in assay buffer and measured for total nitrate and nitrite using Nitrate/Nitrite Colorimetric Assay Kit (Cayman Chemical Cat. No. 780001). Absorbance was measured at 540 nm using a BioTek Synergy H1 Microplate Reader ...
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bioRxiv - Immunology 2023Quote: ... violaceum colony was grown overnight in BHI broth then diluted to 1×106 CFU/mL of PBS and mixed with various concentrations of DEA NONOate (Cayman Chemical Cat. No. 82100) with or without itaconic acid (Sigma-Aldrich Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Trp53 and Smad4 mutations were selected by removing L-WRN conditioned media and adding 10 µm Nutlin-3 (an Mdm2 inhibitor enabling functional selection for Trp53 loss of function) (Cayman Chemical Company #10004372-1). 10 subclones were picked from the engineered bulk organoids ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and (3Z)-5-[(1S,2R,3R,5S)-3,5-dihydroxy-2-[(1E,3S)-3-hydroxy-1-octen-1-yl]cyclopentyl]-3-pentenoic acid (2,3-dinor-8-isoPGF2α, CAS 221664-05-7) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Formic acid was from Merck (Darmstadt ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: Phalloidin staining at E13.5 coronal sections was performed by incubating 1X Phalloidin working solution (1X PBS, 10% BSA and Phalloidin-iFluor™ 488) 1000x conjugate in DMSO (1:1000; Cayman Chemical Company 20549) for 45 minutes ...
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bioRxiv - Plant Biology 2024Quote: Approximately 100 mg of each sample was ground and spiked with an internal standard containing 10 µg nonadecanoic acid (1 mg/mL in ethanol) and JA-d5 (Cayman Chemical, Ann Arbor, MI) (JA-d5 ...
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bioRxiv - Biochemistry 2022Quote: ... Nitrite concentrations were determined by reacting 100 μL aliquots of reaction sample with 50 μL of deoxygenated Griess reagent R1 (Cayman Chemical, 1% sulfanilamide in 5% H3PO4) followed by addition of 50 μL of deoxygenated Griess reagent R2 (Cayman Chemical ...
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bioRxiv - Bioengineering 2024Quote: ... cells were allowed to rest for 5 minutes then added to pre-warmed wells of a 24 well plate containing media (as specified in CD34+ cell culture) supplemented with small molecules as indicated for specific experiments (AZD7648, RS-1, Cayman Chemicals; M-3814, Toronto Research Chemicals). Where AAV donor was used ...
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bioRxiv - Molecular Biology 2022Quote: ... Calcein-AM was purchased from Thermo (Waltham, MA, USA; Cat: C1430) and 1-Ethoxycarbonylmethyl-6-methoxyquinolinium bromide (MQAE) was purchased from Cayman Chemical (Ann Arbor, MI, USA; Cat:19585). Pluronic acid (Pluronic F-127 ...