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Citations for Cayman Chemical :
451 - 492 of 492 citations for BN80933 CAS 214348 10 4 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... SV fusion during stimulation was measured by stimulating sypHy-transfected neurons (10 Hz, 90 s) in the presence of 1 µM bafilomycin A1 (Cayman Chemical Company ...
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bioRxiv - Cell Biology 2023Quote: ... P53 knock-out status of clones was confirmed by testing sensitivity for the p53 stabilizer Nutlin-3a (10 µM for 16h, Cayman Chemical) and Western Blotting for p53 protein.
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bioRxiv - Cell Biology 2023Quote: ... and seeded on 1:30 Matrigel in 12-well plates in TeSR-E8 media supplemented with Y-27632 (10 μM, #10005583, Cayman Chemical). Media was changed to fresh TeSR-E8 after 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... at a density of 30 000 cells/cm2 in a neural precursor cell differentiation medium (NPCM) supplemented with 10 μM Y-27632 (Cayman Chemicals) and 0.1 μM compound E (Cayman Chemicals) ...
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bioRxiv - Neuroscience 2022Quote: ... lysates were diluted 1:5 (cingulate cortex, cerebellum, putamen) or 1:10 (frontal cortex) into assay buffer containing 0.25% sodium taurocholate (Cayman Chemical, 16215), 0.25% triton-X-100 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Single-cell suspensions were resuspended in 100–200 µl (PBS) containing 2.5 μg/ml propidium iodide (PI) (Cayman Chemical #14289-10), for 10 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2023Quote: ... Medium for AF cells after seeding and organoid cultures after splitting additionally contained ROCK inhibitor Y-27632 (10 μM, Cayman Chemical). The AFKO cultures were split on average every 16 days ...
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bioRxiv - Biochemistry 2023Quote: ... The G quadruplex/hemin RNAzyme was allowed to react with 10-Acetyl-3,7-dihydroxyphenoxazine (Amplex Red, Cayman Chemical Company, Cat#10010469) and Hydrogen peroxide for 5 minutes ...
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bioRxiv - Immunology 2022Quote: For the pathway studies neutrophils were incubated for 30 min before stimulation with 10 μM BB-Cl-amidine (PADi, Cayman Chemicals), 15 μM Dipenyleneiodium (DPI ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 1,3,5(10)-estratrien-2,3,17β-triol (2-hydroxyestradiol or 2-OH-E2) were obtained from Cayman Chemical (Ann Arbor, MI, USA). E1 ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were dissociated with accutase and replated at a density of 5 x 105 cells/well in Matrigel-coated 6-well plates using E8 Flex media supplemented with 10 µM Rock inhibitor (Y27632; Cayman Chemical). One day later (day -1) ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were dissociated with accutase and replated at a density of 5.5 x 104 cells/cm2 in Matrigel-coated 24- or 12-well plates or 4.3 x 104 cells/cm2 in Matrigel-coated 6-well plates and 100 mm-dishes using E8 Flex media supplemented with 10 µM Rock inhibitor (Y27632; Cayman Chemical). Next day (day -1) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells at a density of 5 x 105 cells/mL were first treated with 10 µM 8-pCPT-2’-O-Me-cAMP (aka cAMP; Cayman Chemical) in HMI-9 medium at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 10 μl/well of a 10x concentration of coelenterazine 400a (final concentration ∼7.5 μM Cayman Chemical Co., Ann Arbor, MI, USA) was added 5 minutes prior to reading ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 10 μl/well of a 10x concentration of coelenterazine h (final concentration 4.7 μM; Cayman Chemical Co., Ann Arbor, MI, USA) was added 10 minutes prior to reading ...
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bioRxiv - Physiology 2024Quote: ... Sections were then incubated with 10 µM DAz-2: Cy-5 mix (DAz-2, Cayman Chemical, #13382; Cy-5, Lumiprobe, #B30B0) in PBS for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... were transduced with 2.5 x 108 particles of AAV2/DJ-hSyn1-tauP301S-(1N4R)-WPRE-hGHpA per well or treated with PBS vehicle and treated with 10 µM S3QEL2 (Cayman Chemical) or DMSO vehicle during a half-media exchange (with B-27 minus antioxidants) ...
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bioRxiv - Cell Biology 2024Quote: ... human colonoids were cultured in IFE medium supplemented with 10 µM Y-27632 and 5 µM Prostaglandin E2 (PGE2) (CAY14010, Cayman Chemical) 76 for 3-5 days after splitting ...
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bioRxiv - Neuroscience 2022Quote: We validated our physiologically compatible THC solution (1:1:18 THC:cremophor:saline at 2.5, 5, or 10 mg/kg of THC; Cayman Chemicals, Ann Arbor, MI, USA) by measuring plasma levels of THC and two THC metabolites (11-hydroxy-delta-9-THC and 11nor-9carboxy delta-9-THC ...
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bioRxiv - Physiology 2021Quote: ... neurons cultured on glass coverslips were incubated in the presence of 10 μg/ml propidium iodide (PI) (Cayman Chemical, Ann Arbor, USA) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Biochemistry 2023Quote: Purified human PPP1R15A single repeat peptides (UK2997, UK3098) were labelled with 10-fold molar concentration of fluorescein-5-malamide (16383, Cayman Chemical Company) in the presence of 1 mM TCEP at 4 °C overnight ...
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: The same amount of RNP mentioned above was firstly premixed and then added to 1/10 volume of premixed SM102-LNP (Cayman Chemical, USA) according to the manufacturer’s instruction for the mRNA loading ...
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bioRxiv - Immunology 2024Quote: ... then the reaction was quenched by adding an equal volume of ice-cold 2X quenching buffer (DPBS containing 10 mM trolox (Cayman Chemical, 10011659), 20 mM sodium ascorbate (Sigma ...
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bioRxiv - Immunology 2024Quote: 3-D organoids grown for 4 days in stem cell enrichment media comprising organoid growth media without Wnt3a CM and Penicillin/Streptomycin and supplemented with 10 μM CHIR99021 (Cayman Chemicals, UK)) were dissociated into a single cell suspension using TrypLE Express (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... DOX (250 ng/ml) or AP-1 inhibitors (SR11302, Santa Cruz Biotechnology Cat #: sc-204295 at 10 uM concentration; T5224, Cayman Chemical, Cat # ...
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bioRxiv - Developmental Biology 2024Quote: ... were cultured in 2 ml retina culture medium in a 24-well plate with or without 10 µM retinoic acid (Cayman Chemical 11017) and incubated at 37°C in 5% CO2 ...
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bioRxiv - Physiology 2024Quote: ... LMCAs were washed and stimulated with a sub-maximal dose of U-46619 (10−6.5 M, Cayman Chemical, Ann Arbor, MI, USA), once the contraction was stable a dose response curve to acetylcholine (10−10-10−4 M ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then resuspended in 0.5 ml BG11 and incubated with 10 μl calcein-AM (Cayman Chemical, 1 mg ml−1 in DMSO) for 1 h at 30 °C in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... These concentrations ensure that a sufficient number of wells contain a single cell.12 E2 (10 ng/mL, Cayman Chemical, Ann Arbor, MI) or OSM (100 ng/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Spheroids of the small intestine were cultured and expanded in conditioned medium supplemented with 10 μM of Rho-associated protein kinase (ROCK) inhibitor (Y-27632; Cayman Chemicals, Michigan, USA) and 10 μM of transforming growth factor-β (TGF-β ...
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bioRxiv - Developmental Biology 2021Quote: ... aNSCs were cultured for 1 d and the medium was supplemented either with 10 μM GW4869 (Cayman Chemical Co., Ann Arbor, MI, USA) or dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... tubacin (10 and 20µM; Selleckchem, Houston, TX), Nintedanib (24h 2 µM; Selleckchem, Houston, TX) and PGE2 (96hr, 1 μM, Cayman Chemicals, Ann Arbor, MI) or FGF2 (96 h ...
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bioRxiv - Cell Biology 2022Quote: ... were filled up to 2 mL methanol and 1 mL PBS was added containing deuterium-labelled internal standards (200 nM d8-5S-HETE, d4-LTB4, d5-LXA4, d5-RvD2, d4-PGE2 and 10 μM d8-AA; Cayman Chemical/Biomol GmbH) to facilitate quantification and sample recovery ...
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bioRxiv - Cell Biology 2024Quote: ... larvae were pre-incubated for 30-45 or 180 min in hypotonic(Π=10 mOsm) E3 supplemented with the following compounds: 50μM Licofelone (Cayman Chemical Company, 100007692), 10μM MK886 (Cayman Chemical Company ...
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bioRxiv - Microbiology 2021Quote: ... The fixed cells were pelleted by centrifugation for 1 min at 4000 rpm then resuspended in PBS with 10 μg/mL Hoechst 33342 (Cayman Chemical; Item no. 15542). Aliquots of stained cells were loaded onto glass microscope slides and visualized using an Olympus IX70 Epifluorescent Microscope fitted with a Hamamatsu C11440 camera.
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bioRxiv - Biophysics 2022Quote: ... were induced along the PM of cultured HeLa cells by adding 10-15% ethanol (25) or 5 µM cytochalasin D (Cayman Chemical, Ann Arbor, MI) to the cell growth medium and incubating for ∼15 min at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... 1mL of the AggreWell medium was manually replaced with supplemented medium containing WNT inhibitor (IWR1-ε, 3 µM, Cayman Chemical, 13659, days 1-10) and Nodal/Activin inhibitor (SB431542 ...
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bioRxiv - Cancer Biology 2022Quote: ... Trp53 and Smad4 mutations were selected by removing L-WRN conditioned media and adding 10 µm Nutlin-3 (an Mdm2 inhibitor enabling functional selection for Trp53 loss of function) (Cayman Chemical Company #10004372-1). 10 subclones were picked from the engineered bulk organoids ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: Phalloidin staining at E13.5 coronal sections was performed by incubating 1X Phalloidin working solution (1X PBS, 10% BSA and Phalloidin-iFluor™ 488) 1000x conjugate in DMSO (1:1000; Cayman Chemical Company 20549) for 45 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... resuspended in PBS pH 7.4 plus 1 mM CaCl2 (ice-cold; 1 mL) and stimulated with the Ca2+-ionophore A23187 (Cayman Chemical; 2.5 µM, 10 min) at 37 °C.
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bioRxiv - Plant Biology 2024Quote: Approximately 100 mg of each sample was ground and spiked with an internal standard containing 10 µg nonadecanoic acid (1 mg/mL in ethanol) and JA-d5 (Cayman Chemical, Ann Arbor, MI) (JA-d5 ...
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bioRxiv - Cell Biology 2022Quote: ... were first mixed with 2 mL of ice-cold methanol containing deuterium-labelled internal standards (200 nM d8-5S-HETE, d4-LTB4, d5-LXA4, d5-RvD2, d4-PGE2 and 10 μM d8-AA; Cayman Chemical/Biomol GmbH, Hamburg, Germany) to facilitate quantification and sample recovery ...