5 Chloro 1 vinyl 2 pyridone citations for Cayman Chemical

Quote: ... cells media was changed to DMEM containing 2% charcoal striped and dialyzed media with 0.1 uM of T0901317 and/or 1uM of LG100268 (noted LG268) (Cayman Chemical, USA). After overnight treatment ...

Palmitoylation targets the Calcineurin phosphatase to the Phosphatidylinositol 4-kinase complex at the plasma membrane

Idil Ulengin-Talkish, et al., bioRxiv - Biochemistry 2021

Quote: ... media was replaced with DMEM containing 2% FBS and labelled with 30 μM 17-ODYA (17-Octadecynoic Acid,34450, Cayman Chemicals) or DMSO for 3hr at 37°C incubator ...

A palmitate-rich metastatic niche enables metastasis growth via p65 acetylation

Patricia Altea-Manzano, et al., bioRxiv - Cancer Biology 2022

Quote: ... Etomoxir and the KAT2A specific inhibitor cyclopentylidene-[4-(4’-chlorophenyl)thiazol-2-yl)hydrazone (CPTH2)79 were purchased from Cayman Chemical, dissolved in ethanol and used at concentrations of 50 and 2 μM ...

APC and P53 mutations synergize to create a therapeutic vulnerability to NOTUM inhibition in advanced colorectal cancer

Yuhua Tian, et al., bioRxiv - Cancer Biology 2022

Quote: ... and seeded at a density of 500,000 cells per well of a matrigel-coated 6-well plate in iPS-Brew XF supplemented with 2 uM Thiazovivin ROCK inhibitor (Cayman Chemical). Definitive endoderm was derived using STEMdiff™ Definitive Endoderm Kit (STEMCELL Technologies ...

Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

KayLee K. Steiner, et al., bioRxiv - Immunology 2024

Quote: ... Cells were then washed in PBS and resuspended at 1×106 cells/mL in fresh media and pre-treated for 2 hours with 0.5mM Glutathione ethyl ester (Et-GSH, Cayman Chemical, 14953) or 50nM UAMC-3203 (Cayman Chemical ...

Discovery of Novel and Selective GPR17 Antagonists as Pharmacological Tools for Developing New Therapeutic Strategies in Diabetes and Obesity

Hu Zhu, et al., bioRxiv - Pharmacology and Toxicology 2024

Quote: Leukotriene D4 (LTD4, Cat# 20310) and 2-Methylthioadenosine diphosphate (2MeS-ADP, Cat# 21230) were purchased from Cayman Chemical (Ann Arbor, Michigan). HAMI 3379 was purchased from MedChemExpress (Monmouth Junction ...

Spatiotemporal coordination of actin regulators generates invasive protrusions in cell-cell fusion

Yue Lu, et al., bioRxiv - Cell Biology 2024

Quote: ... the cells were trypsinized and plated at 40% confluence on fibronectin-coated cover glass in regular GM containing 1μM ERK1/2 inhibitor (Cayman Chemicals; SCH772984). Subsequently ...

Expression of the type III secretion system genes in epiphytic Erwinia amylovora cells on apple stigmas benefits endophytic infection at the hypanthium

Zhouqi Cui, et al., bioRxiv - Molecular Biology 2020

Quote: ... Suspensions were centrifuge at 3,000 g for 5 min and the cAMP levels in supernatants were measured using the cAMP EIA Kit (Cayman Chemical, Ann Arbor, MI, US) according to the manufacturer’s instructions ...

Ventral tegmental area glutamate neurons mediate the nonassociative consequences of traumatic stress

Dillon J. McGovern, et al., bioRxiv - Neuroscience 2021

Quote: ... Baseline firing in response to current injections was collected for 5 min followed by a 10 min bath application of 10 μM clozapine (Cayman Chemical, Ann Arbor, MI). The average number of action potentials generated during five consecutive sweeps was used to quantify baseline activity and the aver-age of five consecutive sweeps during clozapine application was used to quantify firing during hM4Di receptor activation ...

Partitioning of ribonucleoprotein complexes from the cellular actin cortex

Isaac Angert, et al., bioRxiv - Biophysics 2022

Quote: ... were induced along the PM of cultured HeLa cells by adding 10-15% ethanol (25) or 5 µM cytochalasin D (Cayman Chemical, Ann Arbor, MI) to the cell growth medium and incubating for ∼15 min at 37°C and 5% CO2 ...

Minimal genetically encoded tags for fluorescent protein labeling in living neurons

Aleksandra Arsić, et al., bioRxiv - Neuroscience 2021

Quote: ... neurons were washed for 3 h and incubated for 30 min (37 °C, 5% CO2) with either 25 µM spermine-NONOate (a nitric oxide donor; Cayman Chemical, cat. no. 82150) or 25 µM sulpho-NONOate (control compound ...

A neural mechanism for discriminating social threat from social safety

Pegah Kassraian, et al., bioRxiv - Neuroscience 2023

Quote: ... Mice were then injected 30 minutes prior to behavioral experiments intraperitoneally with 5 mg/kg of the iDREADD agonist clozapine-n-oxide (CNO; Cayman Chemical, #34233-69-7) and returned to their homecage until behavioral testing ...

Interaction of sortilin with apolipoprotein E3 enables neurons to use long-chain fatty acids as alternative metabolic fuel

Anna K Greda, et al., bioRxiv - Neuroscience 2024

Quote: ... C18:2-d4, C20:4-d11, C20:5-d5, C22:0-d43, C22:6-d5, C24:0-d4; 10 ng each; Cayman Chemical, Ann Arbor MI). HPLC measurements were performed using an Agilent 1290 HPLC system with binary pump ...

Significant inactivation of SARS-CoV-2 by a green tea catechin, a catechin-derivative and galloylated theaflavins in vitro

Eriko Ohgitani, et al., bioRxiv - Microbiology 2020

Quote: Neutralizing assay was performed using SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA; Item No. 502070) according to the manufacturer’s protocol.

Endothelial Histone Deacetylase 1 Activity Impairs Kidney Microvascular NO Signaling in Rats fed a High Salt Diet

Luke S. Dunaway, et al., bioRxiv - Physiology 2023

Quote: ... The reaction proceeded for 2 hours at 37°C in presence of either 300 nM MS-275 (Cayman Chemical, Ann Arbor, MI) or vehicle (0.01% DMSO) ...

Cannabinoids Activate the Insulin Pathway to Modulate Mobilization of Cholesterol in C. elegans

Bruno Hernandez-Cravero, et al., bioRxiv - Neuroscience 2022

Quote: ... were immobilized in a PDMS microfluidic olfactory chip [58,59] and exposed to either a control buffer or a stimulus buffer containing 100mM 2-AG (Cayman Chemical Co. #62160). The required volume of 2-AG required to make 100mM solution was dissolved in 0.1% ethanol before being added to S-Basal buffer ...

Single cell transcriptomics reveals early photoreceptor states, cell-specific transcript isoforms, and cancer-predisposing features

Dominic W.H. Shayler, et al., bioRxiv - Developmental Biology 2024

Quote: ... were cultured in 2 ml retina culture medium in a 24-well plate with or without 10 µM retinoic acid (Cayman Chemical 11017) and incubated at 37°C in 5% CO2 ...

Gre factors protect against phenotypic diversification and cheating in Escherichia coli populations under toxic metabolite stress

Darshan M. Sivaloganathan, et al., bioRxiv - Microbiology 2023

Quote: ... (Z)-1-[N-(3-aminopropyl)-N-(3-ammoniopropyl)amino]diazen-1-ium-1,2-diolate (DPTA NONOate) (Cayman Chemical), was dissolved in 10 mM NaOH to a concentration of 72 mM and stored on ice ...

The autophagy activator Spermidine reduces neuroinflammation and soluble amyloid beta in an Alzheimer’s disease mouse model

Kiara Freitag, et al., bioRxiv - Neuroscience 2021

Quote: A® 1-42 peptides (Cayman Chemicals) were resuspended in hexafluoroisopropanol to obtain 1 mM solution ...

Synaptic RTP801 Contributes to Motor Learning Dysfunction in Huntington’s Disease

Núria Martín-Flores, et al., bioRxiv - Neuroscience 2020

Quote: ... PHLPP1 rabbit polyclonal (1:500, Cayman Chemical), PSD-95 mouse monoclonal (1:1000 ...

Unique features of magic spot metabolism in Clostridioides difficile

Asia Poudel, et al., bioRxiv - Microbiology 2021

Quote: ... 0.67 µg mL−1 fidaxomicin (Cayman Chemical), 1.0 mM diamide (MP Biomedicals) ...

SKAP2 as a new regulator of oligodendroglial migration and myelin sheath formation

Julia Ghelman, et al., bioRxiv - Neuroscience 2021

Quote: ... 1 µM SAG (#11914, Cayman Chemical Company), 200 µM L-ascorbic acid (#A4544 ...

Labshake search