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Citations for anatrace :
1 - 50 of 63 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding 50 mM n-Dodecyl-β-D-maltopyranoside with 0.25% cholesteryl hemisuccinate (Anatrace, D310-CH210) and nutating for 1 hr at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The bound proteins were then reconstituted on-column into peptidisc22 or amphipol by incubation in 5ml of washing buffer supplemented with 10mg peptidisc peptide (Peptidisc Lab) or amphipol A8-35 (Anatrace) for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... To reconstitute MBOAT7 proteins in PMAL-C8 (Anatrace), MBOAT7 in detergent was mixed with PMAL-C8 at a 1:3 (w:w ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM imidazole and 0.05% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG; Anatrace), then bound protein was eluted with 20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was extracted by incubating with 1% GDN (Anatrace) on a rotator for 2 hours at 4C ...
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bioRxiv - Biophysics 2022Quote: SEC-purified protein [in SEC buffer containing 3 mM DM (Anatrace)] was reconstituted into liposomes ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated protein was screened for crystallization with the MCSG Crystallization Suite (Anatrace). Long ...
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bioRxiv - Biophysics 2022Quote: ... Protein was solubilized with the addition of 0.1% (v/v) DDM/CHS (Anatrace) for 2 hours at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to a final concentration of 2% ...
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bioRxiv - Cell Biology 2023Quote: ... Expressed protein was solubilized in 2 % lauryl maltose neopentyl glycol (LMNG) (Anatrace, NG310), 0.2 % Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Neuroscience 2024Quote: ... protein was extracted from the pellet with 0.5% Fos-choline 14 (#F312, Anatrace) at 4°C for 40 minutes and centrifuged at 21 000 g for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in 500 μl solubilization buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 1% (w/v) DDM (Anatrace), protease inhibitor cocktail set III (Merck Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were collected and centrifuged at 500 x g for 3 minutes, resuspended with lysis buffer (150 mM NaCl, 50 mM HEPES, pH 7.5, 1% (w/v) DDM (Anatrace), 1 mM PMSF) ...
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bioRxiv - Biochemistry 2021Quote: ... pre-equilibrated with size exclusion buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 1 mM DDM (Anatrace)) in line with a fluorescence detector (RF-20A ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 8 column buffers of GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.006 % (w/v) glyco-diosgenin (GDN, Anatrace), pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein-bound resin was washed with 10 mM imidazole and eluted with imidazole (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 20 % glycerol, 0.1% DDM (Anatrace, USA), 1 M imidazole) ...
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bioRxiv - Biophysics 2022Quote: ... before being resuspended in buffer (50 mM Tris-HCl pH 7.4, 10% [v/v] glycerol, 1% [w/w] DDM [Anatrace] ...
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bioRxiv - Biophysics 2023Quote: ... and the cell pellets were resuspended in lysis buffer (50 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 5 mM MgCl2, 20 mM imidazole, 1mM CHAPS (Anatrace), 5mM 2-mercaptoethanol ...
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bioRxiv - Biophysics 2020Quote: 3.5 μL of protein-embedded nanodiscs supplemented with 1.5 mM Fos-Choline-8 (Anatrace) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Biophysics 2022Quote: The pelleted membrane fractions were then solubilized in resuspension buffer (50 mM Tris/Mes, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5) containing 1% β-DDM (Anatrace) and stirred on ice for 60minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed in ATF6 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 1x protease inhibitor, and 1% LMNG (Anatrace NG322) for 15 min at 4°C as previously described68 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were solubilized by resuspending in buffer (50 mM Tris-HCl, pH 7.4, 10% [v/v] glycerol, 1% [w/w] dodecyl-β-D-maltoside [DDM, Anatrace] ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was lysed in 500 μl of lysis buffer (50 mM Tris-HCl pH 7.2, 10% v/v glycerol, 150 mM NaCl, 1% w/v DIBMA (Anatrace BMA101), with protease inhibitors (1% v/v ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction pellets were solubilized in solubilization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2% n-dodecyl-beta-d-maltopyranoside (DDM) (Anatrace, USA) with 1 mM PMSF ...
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bioRxiv - Biophysics 2022Quote: ... for 30 min at 4 °C and then solubilized by addition of 2× buffer (50 mM HEPES, 500 mM NaCl, 2 %w/v n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.4 %w/v cholesteryl hemisuccinate (CHS ...
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bioRxiv - Neuroscience 2019Quote: ... Cell powder was added to lysis buffer (50 mM Tris pH 8.0, 150 mM KCl, 60 mM dodecyl-β-D-maltoside (DDM, Anatrace Inc.), 100 μg/ml DNAse 1 ...
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bioRxiv - Biochemistry 2022Quote: ... diluted with 4 mL GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.004% [w/v] glyco-diosgenin [GDN; Anatrace], pH 7.4) and concentrated to ~200 μL in the same concentrator ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 8 column buffers of GTBS (50 mM Tris HCl, 150 mM NaCl, 0.006% [w/v] glyco-diosgenin [GDN; Anatrace], pH 8.0). Ycf1p was eluted with 6 column volumes of GTBS containing 150 μg/mL 3×FLAG peptide ...
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bioRxiv - Plant Biology 2021Quote: Thylakoids (100 µg of proteins) were solubilized with 0.75% n-Dodecyl-α-D-maltoside (Anatrace) at RT for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein was extracted by 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Protein extraction was carried out with 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace #D322S25GM) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... protein was extracted with 2% (w/v) decyl-ý-D-maltopyranoside (DM; Anatrace, Maumee, OH) for 2 h at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... Membrane pellets were scooped into a dounce homogenizer containing GDN Extraction Buffer (50 mM HEPES, 150 mM KCl, 1 mM EDTA, 1% Glyco-diosgenin (GDN: Anatrace, Maumee, OH), and all lysis buffer protease inhibitors except PMSF ...
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bioRxiv - Microbiology 2019Quote: ... Outer-membrane proteins were solubilized using 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in a buffer containing 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Neuroscience 2021Quote: ... Protein was extracted with a final concentration of 1% n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.2% cholesteryl hemisuccinate (CHS ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... Protein was extracted by addition of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was solubilized by addition of 1% w/v n-decyl-β-D-maltopyranoside (DM) (Anatrace) and 0.1% w/v cholesteryl hemisuccinate (CHS ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was discarded and membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
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bioRxiv - Biophysics 2022Quote: ... Membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM HEPES, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Biophysics 2021Quote: ... Membrane pellets were scooped into a dounce homogenizer containing Extraction Buffer (50 mM Tris, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... The cell pellet was re-dissolved and dounced in solubilization buffer (50 mM Tris-Cl, pH 8.0, 150 mM NaCl, 5 mM β-mercaptoethanol, 1% DDM (Anatrace, Cat. no. D310), 1 mM PMSF ...
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bioRxiv - Cell Biology 2021Quote: His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... and membrane proteins were extracted by stirring with 30 mM n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was concentrated to 10 mg/ml using a Proteus X-spinner with 10 kDa filter (Anatrace) and snap frozen in liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... membrane proteins were extracted from the cell with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 μL of 10% DDM (β-D-dodecyl maltoside, Anatrace) and 13 μL of 27mM mal-PEG (Methoxypolyethylene glycol maleimide ...
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bioRxiv - Biophysics 2020Quote: ... The mutant CLC-ec1 proteins were extracted with n-decyl-β-D-maltopyranoside (DM, Anatrace; 2% (w/v)) and allowed to bind to cobalt resin (Talon ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were extracted by supplementing the lysis buffer with 1% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), and incubated for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 µl of 10% β-d-dodecyl maltoside (Anatrace 329370010) and 13 µl of freshly dissolved 27 mM 5 kDa mal-PEG ...