Labshake search
Citations for anatrace :
201 - 250 of 315 citations for Who Coplanar & Mono Ortho Pcb + Pcb 170 & Pcb 180 13C12 99% 20 Ng Ml In N Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... membranes were thawed on ice and solubilized using 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM, Anatrace), 0.2% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... Immobilization buffer was 10 mM HEPES buffer pH 7.4 containing 0.05% n-octyl-β-D-glucopyranoside (OGPS, Anatrace), 150 mM NaCl2 ...
-
bioRxiv - Biochemistry 2021Quote: ... Solubilization of cell membranes was achieved by incubating with 30 mM n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace) for 2 h at 4°C under gentle agitation ...
-
bioRxiv - Microbiology 2020Quote: ... The reaction was quenched after 60 minutes at RT with 0.5 μL of 4.8% Lauryl Dimethylamine-N-Oxide (Anatrace), followed by addition of 6 μL Detection Solution ...
-
bioRxiv - Biophysics 2022Quote: Solubilization was done with drop-wise addition of resuspension buffer with 10% n-octyl-β-D-glucoside (Anatrace) to a final concentration of 5% ...
-
bioRxiv - Biophysics 2022Quote: ... XWnt8 was eluted with HBS containing 0.1% (v/v) n-dodecyl β-D-maltoside (DDM, Anatrace, Sol-Grade) and 500 mM NaCl ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane pellet was homogenized in buffer E supplemented with 1% n-Decyl-β-D-Maltopyranoside (DM, Anatrace) for YidC or n-Dodecyl-N ...
-
bioRxiv - Biochemistry 2022Quote: ... AcrAL was solubilised from homogenised membranes with 1% (w/v) n-dodecyl-β-D-maltoside (DDM) detergent (Anatrace) at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were extracted by supplementing the lysis buffer with 1% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), and incubated for 1.5 h at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... The detergents n-dodecylmaltoside (DDM) and lauryl maltose neopentyl glycol (LMNG) and the amphipol A8-35 were from Anatrace. p-benzoyl-phenylalanine was from Bachem ...
-
bioRxiv - Biophysics 2021Quote: ... Frozen membranes were thawed on ice and extracted by addition of solid n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to a final concentration of 20 mM at 4 °C for 2 h with gentle agitation ...
-
bioRxiv - Biochemistry 2022Quote: ... Heterotrimeric Giβ1γ2 was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Ni- NTA chromatography is performed and the detergent was exchanged from cholate/DDM to DDM on column ...
-
bioRxiv - Biophysics 2020Quote: ... The membrane fraction was collected by centrifugation at 100,000xg and then solubilized by addition of 0.375 g n-dodecyl-β-D-maltoside (DDM, Anatrace) per gram of the membrane pellet for 2 h at 4°C in lysis buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... the liposome sample was sonicated to transparency and incubated with 40 mM n-decyl-β-D-maltoside (DM, Anatrace) for 2 h at room temperature under gentle agitation ...
-
bioRxiv - Biophysics 2023Quote: ... After resuspension in buffer B (buffer A supplemented with 1% (w/v) n-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.15% (w/v ...
-
bioRxiv - Biophysics 2023Quote: ... ConstantSystems) and isolated crude membranes were solubilized with 1.5 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM) (anagrade, Anatrace). Solubilized LeuT was incubated with Ni2+-NTA resin (Thermo Fischer Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... 20 CV WB with 0.06 % (w/v) glyco-diosgenin (GDN) (Anatrace) and 20 CV WB with 0.02 % (w/v ...
-
bioRxiv - Biophysics 2023Quote: ... 20 mM imidazole and 85 μM glyco-diosgenin (GDN, GDN101, Anatrace). The protein was eluted with 200 mM imidazole and digested with PreScission protease at 4 ℃ overnight to remove the C-terminal GFP-His10 tag ...
-
bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
-
bioRxiv - Biophysics 2021Quote: ... with 1 % (v/v) membrane protease inhibitors and 1.0 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM) 40:1 (w/w) CHS (Anatrace). After centrifugation ...
-
bioRxiv - Biophysics 2022Quote: ... The pellet containing the membrane fraction was solubilized in 1% N-dodecyl-β-D-maltopyranoside (DDM) and 0.1% cholesteryl hemisuccinate (CHS; Tris Salt, Anatrace) for 1 hour at 4°C ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding 50 mM n-Dodecyl-β-D-maltopyranoside with 0.25% cholesteryl hemisuccinate (Anatrace, D310-CH210) and nutating for 1 hr at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells membranes were isolated by ultracentrifugation at 200,000 x g (45 min) and solubilised with 40 mM n-dodecyl-β-D-maltopyranoside (DDM, Anatrace). Solubilised membranes were incubated with Ni-NTA resin (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: ... the transfected 293T cells were harvested and lysed in PBS buffer containing 0.3 % n-decyl-β-D-maltopyranoside (DDM, Anatrace). Cell lysates were incubated with Protein A/G PLUS Agarose (Santa Cruz ...
-
bioRxiv - Biophysics 2021Quote: ... The suspension was incubated for 1 h at room temperature and the complex was solubilized from the membrane using 0.5% (w/v) n-dodecyl-β-d-maltoside (DDM, Anatrace) and 0.1% (w/v ...
-
bioRxiv - Microbiology 2023Quote: ... 7[mM NMG (pH 8.0)) and solubilized by adding 40[mM DM (n-Decyl-β-D-Maltoside) (Sol-Grade from Anatrace). KtrB alone ...
-
bioRxiv - Biophysics 2023Quote: ... The membrane fraction pellets were solubilized in solubilization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2% n-dodecyl-beta-d-maltopyranoside (DDM) (Anatrace, USA) with 1 mM PMSF ...
-
bioRxiv - Cell Biology 2023Quote: ... the membranes were solubilized by incubating with an addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace) and 0.2% (w/v ...
-
Structural insight into guanylyl cyclase receptor hijacking of the kinase–Hsp90 regulatory mechanismbioRxiv - Biochemistry 2023Quote: ... Membranes were thawed and solubilized with the addition of 1% n-dodecyl β-D-maltoside (DDM) and 0.1% cholesteryl hemisuccinate (CHS) (10:1) (Anatrace). Debris and unsolubilized membranes were pelleted by centrifugation at 46,000 x g ...
-
bioRxiv - Biophysics 2023Quote: ... Protein was extracted via the addition of n-Dodecyl-β-D-maltopyranoside (DDM) with cholesteryl hemisuccinate (CHS) (Anatrace, D310-CH210) to a final concentration of 50 mM DDM / 4 mM CHS and rotated for 1 hour at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... and receptor was subsequently extracted from membranes in a volume of 50 ml by addition of 2× solubilisation buffer (100 mM HEPES pH 7.5, 250 mM NaCl, 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Giβ1ψ2 G-protein were lysed in hypotonic buffer and G-protein was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Detergent was exchanged from cholate/DDM to DDM on Ni Sepharose column ...
-
bioRxiv - Biochemistry 2020Quote: ... The cells were lysed by cryogenic milling and then solubilized using the detergent n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, solgrade). CST was purified from the clarified lysate using Talon resin (Clontech ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were subsequently incubated for 5 min at 37°C and lysed by the addition of 0.5% N-Dodecyl- ß-D-maltoside (DDM) (Anatrace D310S). Cells were incubated at 37°C for additional 5 to 10 min while monitoring lysis ...
-
bioRxiv - Biophysics 2019Quote: ... together with a surfactant mixture of 1.2 nM of DDM and 1.1 nM n-Octyl-β-D-Glucopyranoside (OG, Anatrace, OH, USA). The protein was incubated for 2 hours before removal of the surfactant ...
-
bioRxiv - Biophysics 2022Quote: ... and the membrane pellet was resuspended in Lysis buffer and the protein was solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.04% cholesteryl hemisuccinate (CHS ...
-
bioRxiv - Biochemistry 2020Quote: AcrB was extracted from homogenized membranes by overnight incubation with 1 % (w/v) n-dodecyl-β-D-maltoside (DDM) detergent (Anatrace) at 4 °C with gentle agitation ...
-
bioRxiv - Cell Biology 2021Quote: ... Two membrane pellets (corresponding to half a fermenter growth) were thawed and solubilized with addition of n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to 1% (w/v ...
-
bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biophysics 2022Quote: ... for 30 min at 4 °C and then solubilized by addition of 2× buffer (50 mM HEPES, 500 mM NaCl, 2 %w/v n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.4 %w/v cholesteryl hemisuccinate (CHS ...
-
bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
-
bioRxiv - Microbiology 2022Quote: ... and extracted with detergent by adding drop-wise a 10% (w/v) stock solution of n-dodecyl β-D-maltoside (DDM, Anatrace) to a final concentration of 1.3% (w/v) ...
-
bioRxiv - Immunology 2020Quote: ... RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological ...
-
bioRxiv - Plant Biology 2022Quote: ... Then the suspension was incubated in the buffer with additional l.5 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) at 4 °C for 2 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [HN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biochemistry 2022Quote: HEK293F cells (for SBA clarified lysate preparation) and HEK293T cells (for expression analysis by Western Blot) were solubilized with n-Dodecyl-β-d-maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
-
bioRxiv - Biophysics 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biochemistry 2023Quote: Frozen membranes were thawed in a tepid water bath and solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM; Anatrace) with mixing for ∼1 h ...
-
bioRxiv - Biophysics 2023Quote: ... washed once with PBS and were resuspended with 500 μL buffer A containing 1% N-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.1% cholesteryl hemisuccinate (CHS ...
-
bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at room temperature (RT) for 1 hour and the membranes were solubilised by adding 1% (w/v) n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace), and 0.2% (w/v ...