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Citations for anatrace :
1 - 50 of 72 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and resuspended in buffer C and 40 mM sodium cholate (Anatrace) at a final lipid concentration of 20 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was concentrated to 10 mg/ml using a Proteus X-spinner with 10 kDa filter (Anatrace) and snap frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... was added before cruel-extraction at 4°C in 1.5% DDM and 0.3% CHS (Anatrace) for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... The plasma membranes were solubilized at 4 °C with 10x critical micelle concentration (CMC) DDM (Anatrace) in a solution of 20mM HEPES (pH8.0) ...
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bioRxiv - Biochemistry 2024Quote: ... Ligand-coated anti-Flag beads were serially 3x washed with Buffer A containing 0.5 mM DDM (Anatrace) and eluted with Buffer A containing 0.5 mM DDM and 250 µg/ml of Flag peptide (SinoBiological) ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... The resin was subsequently washed with buffer C (buffer A supplemented with 0.03% (w/v) glycol-diosgenin (GDN, Anatrace)) for 10 column volumes ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently incubated for 5 min at 37°C and lysed by the addition of 0.5% N-Dodecyl- ß-D-maltoside (DDM) (Anatrace D310S). Cells were incubated at 37°C for additional 5 to 10 min while monitoring lysis ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biophysics 2024Quote: ... N-dodecyl-β-D-maltoside (DDM) and Anapoe® X-100 (Triton® X-100) were purchased from Anatrace (Maumee, OH, USA). 50 mesh BioBeads® were purchased from Bio-Rad (Hercules ...
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bioRxiv - Cell Biology 2023Quote: ... Triton X-100™ (Anatrace, Ref. T1001-500 mL), DAB (Sigma Aldrich ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... containing 0.1% (v/v) Triton X-100 (Anatrace, catalog no. APX100) nonionic surfactant and 500 μL Tris 7.0 buffer containing 0.1% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... Lipids were completely solubilized in 1.2% (w/v; 14.8mM) Triton X-100 (Anatrace) for 20 min at room temperature ...
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Structural insight into guanylyl cyclase receptor hijacking of the kinase–Hsp90 regulatory mechanismbioRxiv - Biochemistry 2023Quote: ... 0.0005% CHS (10:1) (Anatrace), and 5 mM ATP ...
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bioRxiv - Biophysics 2022Quote: ... 10% C12M (Anatrace, Maumee, OH, USA) stock solution was mixed into membrane homogenate to a final concentration of 2% ...
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bioRxiv - Biophysics 2022Quote: ... the membrane fraction was prepared by ultracentrifugation at 100,000 x g for one hour and protein was solubilized by addition of lysis buffer containing 0.5 % LMNG (Anatrace) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... protein sample was spun down at 290,000 x g for 20 min and mixed with either n-octyl-β-D-glucopyranoside (OG; Anatrace) or n-nonyl-β-D-glucoside (NG ...
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bioRxiv - Neuroscience 2021Quote: ... Cells membranes were isolated by ultracentrifugation at 200,000 x g (45 min) and solubilised with 40 mM n-dodecyl-β-D-maltopyranoside (DDM, Anatrace). Solubilised membranes were incubated with Ni-NTA resin (Qiagen ...
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bioRxiv - Biophysics 2022Quote: ... 0.005 w/v% LMNG-CHS (10:1, Anatrace), and 0.0004 w/v% GDN ...
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bioRxiv - Biochemistry 2022Quote: ... and DDM-CHS (10:1 pre-mix, Anatrace) was then added to a final concentration of 1 % (w/v) ...
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bioRxiv - Biophysics 2020Quote: ... 10 μg of OlZAC (10 μl) was mixed with 200 μg of NAPol (Anatrace, dissolved in 2 µl of buffer B) and incubated for 16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1% sol-grade dodecylmaltoside (DDM, Anatrace), mixed at 4°C for 1 hour and ultracentrifuged ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μM GHRH and 0.001% (w/v) digitonin (Anatrace). The monomeric fractions of the SV1–Gs complex were collected and concentrated to 10-20 mg/mL for cryo-EM examination.
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bioRxiv - Biophysics 2022Quote: ... and 0.1 w/v% LMNG-CHS (10:1, Anatrace), pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1 w/v% LMNG-CHS (10:1, Anatrace), pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace, Maumee, OH) and incubating for 1 h at 4° C ...
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bioRxiv - Biophysics 2021Quote: ... then destabilized with 10 mM Fos-choline 12 (Anatrace Inc). For liposome flux assay ...
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bioRxiv - Biochemistry 2020Quote: ... 1 ml 10 % (w/v) lauryl maltose neopentyl glycol (LMNG; Anatrace) solution was added per g of membranes and the suspension was gently stirred at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... the 10% DDM stock was supplemented with 2% CHS (Anatrace, Cat#CH210), which resulted in a final lipid:CHS molar ratio of ∼60:40 ...
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bioRxiv - Biochemistry 2023Quote: ... Lipids were solubilized in 10% (w/v) lauryl maltose neopentyl glycol (Anatrace) by vortexing for 4 hours at room temperature to give 10 mg mL-1 lipid in a 10% (w/v ...
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bioRxiv - Biophysics 2024Quote: Membranes (10 mg/mL) were solubilized with 1% glyco-diosgenin (GDN, Anatrace), a synthetic drop-in substitute for Digitonin ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM; Anatrace) by stirring at room temperature for 1h ...
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bioRxiv - Biophysics 2021Quote: n-Decyl- and n-dodecylphosphocholine (Fos-Choline-10 and DPC) were obtained from Anatrace Products (Maumee ...
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bioRxiv - Immunology 2019Quote: ... we added to the pooled Strepactin elute a 10% solution of PMAL-C16 (Anatrace, Maumee, OH), dropwise with mixing ...
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bioRxiv - Biophysics 2020Quote: ... Membranes were first isolated and solubilized with 10 mM n-Dodecyl-β-D-maltoside (C12M; Anatrace) in binding buffer containing 20 mM HEPES-Tris (pH 7.5) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 30 mL of 10X Detergent buffer [10% (w/v) lauryl maltose neopentyl glycol (L-MNG; Anatrace) and 1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... KimA at a concentration of 12 mg/ml and amphipols PMAL C8 (10% in water; Anatrace) were incubated at w/w ratio of 1:10 for 1 h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μM E-64 and 5 mM Benzamidine HCl) containing 1% Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% cholesteryl hemisuccinate (CHS ...