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Citations for anatrace :
1 - 50 of 55 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... receptor was solubilized in 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... was performed to separate receptor-nanobody complex from excess nanobody and to exchange the detergent from DM to HEGA-10 (Anatrace) for crystallization by vapour diffusion ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biochemistry 2024Quote: ... and resuspended in buffer C and 40 mM sodium cholate (Anatrace) at a final lipid concentration of 20 mM ...
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bioRxiv - Biochemistry 2020Quote: ... was added before cruel-extraction at 4°C in 1.5% DDM and 0.3% CHS (Anatrace) for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... The plasma membranes were solubilized at 4 °C with 10x critical micelle concentration (CMC) DDM (Anatrace) in a solution of 20mM HEPES (pH8.0) ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... The resin was subsequently washed with buffer C (buffer A supplemented with 0.03% (w/v) glycol-diosgenin (GDN, Anatrace)) for 10 column volumes ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently incubated for 5 min at 37°C and lysed by the addition of 0.5% N-Dodecyl- ß-D-maltoside (DDM) (Anatrace D310S). Cells were incubated at 37°C for additional 5 to 10 min while monitoring lysis ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Biochemistry 2023Quote: ... and PACT kits (Molecular Dimensions, Anatrace, Inc.) at 20 ℃ ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... crystal conditions screened using the MCSG2 crystallization kit (Anatrace Products). X-ray quality crystals grew after 24 hours at 4 ℃ using the condition ...
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bioRxiv - Biochemistry 2022Quote: ... protein complexes were overexpressed in BL21(DE3) cells and then extracted from cell membranes using lauryl maltose neopentyl glycol (LMNG; Anatrace). Protein complexes were then affinity-purified using StrepTactin XT resin (IBA ...
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bioRxiv - Biochemistry 2024Quote: ... BtuG1 was screened for crystals using the MCSG1 crystallization kit (Anatrace Products). Several hits were observed after 24 hours at 4℃ ...
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bioRxiv - Biochemistry 2019Quote: Cells were solubilized with dodecyl maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
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bioRxiv - Biophysics 2021Quote: ... coli BL21(DE3) RP cells and purified in DDM (Anatrace) (42) ...
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bioRxiv - Biophysics 2019Quote: ... Cell membranes were solubilized by addition of LMNG or Cymal5-NG (Anatrace) to a final concentration of 0.5 % (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were solubilized by supplementing dodecyl maltoside (DDM) (Anatrace, Sol-grade) to final concentrations of cell at 20 % (w/v ...
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bioRxiv - Biophysics 2021Quote: ... Cell membrane was solubilized in n-dodecyl-b-D-maltoside (DDM, Anatrace) and 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS ...
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bioRxiv - Neuroscience 2019Quote: ... cells were homogenized in the ice cold lysis buffer comprising 1.5% DDM (Anatrace), 10% Glycerol (Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... Whole cells were solubilized overnight in 1% Lauryl Maltose Neopentyl Glycol (LMNG Anatrace) and 0.2% Cholesteryl Hemisuccinate (CHS ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were solubilized with solubilization buffer containing 1% glyco-diosgenin (GDN) (Anatrace, USA). The wash buffer contained 0.05% [w/v] GDN ...
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bioRxiv - Biochemistry 2023Quote: ... Cell membranes were solubilized with 30 mM n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace). Home-made eGFP nanobody-crosslinked resins (NHS-Activated Sepharose 4 Fast Flow ...
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bioRxiv - Biophysics 2020Quote: ... The cell mixture was extracted with 1% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) for 2 h with stirring at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Lysed cells were solubilized by continuous stirring in 1% L-MNG (Anatrace, Cat. no. NG310) for two hours at 4 °C in the presence of 0.01% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysis was then solubilized in 0.5% (w/v) lauryl maltose neopentylglycol (LMNG, Anatrace), and 0.1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were solubilized in detergent (1 % (w/v) n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace), n-Decyl-β-D-Maltopyranoside (DM ...
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bioRxiv - Biochemistry 2024Quote: ... BtuG2 was concentrated to 22 mg/mL prior to substrate addition and screened for crystals using the MCSG3 crystallization kit (Anatrace Products). Several hits were observed after 48 hours at 4 ℃ using the following condition ...
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bioRxiv - Biophysics 2020Quote: ... Cell membranes were solubilized in buffer containing 1% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) for 2 h with stirring at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was supplemented with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Cell membranes were solubilized with 1% (w/v) n-dodecyl β-D-maltopyranoside (#D3105GM, DDM, Anatrace) for 1h ...
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bioRxiv - Biophysics 2023Quote: ... The cell mixture was extracted with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, NG310, Anatrace) for 2 hours with stirring at 4℃ and then centrifuged for 1 hour at 30,000g ...
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bioRxiv - Biochemistry 2023Quote: ... Infected Hi5 cells were collected at 72 hr post infection and lysed in 2% CHAPSO (Anatrace) buffer (25 mM Pipes pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed by the addition of 1% v/w n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and by stirring the homogenate for 3 hr at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... membrane proteins were extracted from the cell with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... Solubilization of cell membranes was achieved by incubating with 30 mM n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace) for 2 h at 4°C under gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... the transfected 293T cells were harvested and lysed in PBS buffer containing 0.3 % n-decyl-β-D-maltopyranoside (DDM, Anatrace). Cell lysates were incubated with Protein A/G PLUS Agarose (Santa Cruz ...
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bioRxiv - Biophysics 2023Quote: ... Cell powder was added at a ratio of 1 g cell powder to 3 ml lysis buffer (200 mM KCl, 21 mM OGNG (octyl glucose neopentyl glycol, Anatrace), 30 mM HTG (n-heptyl-β-D-thioglucopyranoside ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were lysed by cryogenic milling and then solubilized using the detergent n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, solgrade). CST was purified from the clarified lysate using Talon resin (Clontech ...