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Citations for anatrace :
1 - 50 of 59 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The bound proteins were then reconstituted on-column into peptidisc22 or amphipol by incubation in 5ml of washing buffer supplemented with 10mg peptidisc peptide (Peptidisc Lab) or amphipol A8-35 (Anatrace) for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... and resuspended in buffer C and 40 mM sodium cholate (Anatrace) at a final lipid concentration of 20 mM ...
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bioRxiv - Biochemistry 2022Quote: ... To reconstitute MBOAT7 proteins in PMAL-C8 (Anatrace), MBOAT7 in detergent was mixed with PMAL-C8 at a 1:3 (w:w ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was extracted by incubating with 1% GDN (Anatrace) on a rotator for 2 hours at 4C ...
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bioRxiv - Biophysics 2022Quote: SEC-purified protein [in SEC buffer containing 3 mM DM (Anatrace)] was reconstituted into liposomes ...
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bioRxiv - Biochemistry 2020Quote: ... was added before cruel-extraction at 4°C in 1.5% DDM and 0.3% CHS (Anatrace) for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated protein was screened for crystallization with the MCSG Crystallization Suite (Anatrace). Long ...
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bioRxiv - Neuroscience 2020Quote: ... The plasma membranes were solubilized at 4 °C with 10x critical micelle concentration (CMC) DDM (Anatrace) in a solution of 20mM HEPES (pH8.0) ...
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bioRxiv - Biophysics 2022Quote: ... Protein was solubilized with the addition of 0.1% (v/v) DDM/CHS (Anatrace) for 2 hours at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to a final concentration of 2% ...
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bioRxiv - Cell Biology 2023Quote: ... Expressed protein was solubilized in 2 % lauryl maltose neopentyl glycol (LMNG) (Anatrace, NG310), 0.2 % Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Neuroscience 2024Quote: ... protein was extracted from the pellet with 0.5% Fos-choline 14 (#F312, Anatrace) at 4°C for 40 minutes and centrifuged at 21 000 g for 1 hour at 4°C ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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bioRxiv - Biophysics 2020Quote: 3.5 μL of protein-embedded nanodiscs supplemented with 1.5 mM Fos-Choline-8 (Anatrace) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 ...
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bioRxiv - Plant Biology 2021Quote: Thylakoids (100 µg of proteins) were solubilized with 0.75% n-Dodecyl-α-D-maltoside (Anatrace) at RT for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein was extracted by 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Protein extraction was carried out with 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace #D322S25GM) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... protein was extracted with 2% (w/v) decyl-ý-D-maltopyranoside (DM; Anatrace, Maumee, OH) for 2 h at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... The resin was subsequently washed with buffer C (buffer A supplemented with 0.03% (w/v) glycol-diosgenin (GDN, Anatrace)) for 10 column volumes ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Outer-membrane proteins were solubilized using 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in a buffer containing 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Neuroscience 2021Quote: ... Protein was extracted with a final concentration of 1% n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.2% cholesteryl hemisuccinate (CHS ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... Protein was extracted by addition of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was solubilized by addition of 1% w/v n-decyl-β-D-maltopyranoside (DM) (Anatrace) and 0.1% w/v cholesteryl hemisuccinate (CHS ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently incubated for 5 min at 37°C and lysed by the addition of 0.5% N-Dodecyl- ß-D-maltoside (DDM) (Anatrace D310S). Cells were incubated at 37°C for additional 5 to 10 min while monitoring lysis ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Cell Biology 2021Quote: His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... and membrane proteins were extracted by stirring with 30 mM n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was concentrated to 10 mg/ml using a Proteus X-spinner with 10 kDa filter (Anatrace) and snap frozen in liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... membrane proteins were extracted from the cell with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 μL of 10% DDM (β-D-dodecyl maltoside, Anatrace) and 13 μL of 27mM mal-PEG (Methoxypolyethylene glycol maleimide ...
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bioRxiv - Biophysics 2020Quote: ... The mutant CLC-ec1 proteins were extracted with n-decyl-β-D-maltopyranoside (DM, Anatrace; 2% (w/v)) and allowed to bind to cobalt resin (Talon ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were extracted by supplementing the lysis buffer with 1% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), and incubated for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 µl of 10% β-d-dodecyl maltoside (Anatrace 329370010) and 13 µl of freshly dissolved 27 mM 5 kDa mal-PEG ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biophysics 2022Quote: ... the membrane fraction was prepared by ultracentrifugation at 100,000 x g for one hour and protein was solubilized by addition of lysis buffer containing 0.5 % LMNG (Anatrace) for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the GLUT3 protein was purified in 0.06% (w/v) 6-cyclohexyl-1-hexyl-ß-D-maltoside (Cymal-6, Anatrace) and concentrated to ~ 40 mg/ml ...
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bioRxiv - Biophysics 2020Quote: ... Concentrated PilQ (150 µL of ∼1 mg/mL protein) was exchanged into Amphipol A8-35 (0.585 mg for a 3:1 ratio, Anatrace, A835) and allowed to incubate at 4 C for 1 hr ...
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bioRxiv - Biophysics 2021Quote: A total of 4.0 μl of 20 μM TpCorC protein in 20 mM HEPES (pH 7.5) containing 0.03% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, USA) and either 150 mM NaCl or 150 mM KCl was mixed with 0.5 μl of 2 mM EDTA ...
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding 50 mM n-Dodecyl-β-D-maltopyranoside with 0.25% cholesteryl hemisuccinate (Anatrace, D310-CH210) and nutating for 1 hr at 4°C ...