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Citations for anatrace :
1 - 50 of 375 citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... 4 mL (1/10 volume) of 1 M n-octyl-β-D-glucopyranoside in H2O (β-OG, Anatrace) was added to 100 mM final concentration ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... Drops of 450 nL of 10 mg/mL protein in SEC buffer and 150 nL of MCSG-1 H10 (Anatrace; 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Biophysics 2020Quote: ... 10 μg of OlZAC (10 μl) was mixed with 200 μg of NAPol (Anatrace, dissolved in 2 µl of buffer B) and incubated for 16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2020Quote: TRPC4 membranes were solubilized for 2 hr in buffer B supplemented with 1% LMNG (Anatrace #NG310). Then a protocol similar to that used for DDM purification was followed ...
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bioRxiv - Biochemistry 2020Quote: Membranes were solubilized for 2 hr in buffer B supplemented with 1% dodecyl maltoside (Anatrace #D310). Insoluble material was removed by ultracentrifugation for 1 hr in a Beckman Coulter Type 70 Ti rotor at 40,000 rpm ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM Cholesteryl Hemisuccinate (CHS, Anatrace) and 2 μM RTx and incubated at 4° C for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... 5 mM n-Decyl-B-D-Maltopyranoside (DM, Anatrace, Maumee, OH), pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: Native and selenomethionine-labeled LapD2 was concentrated to 10 mg/ml and screened for crystallization conditions using the MCSG1-4 crystallization suites (Anatrace) and the hanging drop vapour diffusion method ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 1% n-Dodecyl-b-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% Cholesterol Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biochemistry 2021Quote: ... 1% n-Dodecyl-b-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% Cholesterol Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biophysics 2020Quote: ... Thawed membranes were solubilized in 1% b-DDM (Anatrace, D310) nutating at 4°C for 1 hour then centrifuged to separate solubilized membranes from the pellet ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μM E-64 and 5 mM Benzamidine HCl) containing 1% Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biophysics 2023Quote: ... and then solubilized with 4% n-octyl-β-D-glucopyranoside (OG; Anatrace). Solubilized AQP0 was purified using anion-exchange (MonoQ ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% (wt/vol) Cymal-5 (Anatrace), and a cocktail of protease inhibitors (Roche Complete EDTA-free tablets) ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biochemistry 2023Quote: Thawed membranes were solubilized with 1% (w/v) dodecyl-B-D-maltoside (DDM) (Anatrace). Insoluble material was removed by centrifugation at 200,000 g for 60 min ...
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Structural insight into guanylyl cyclase receptor hijacking of the kinase–Hsp90 regulatory mechanismbioRxiv - Biochemistry 2023Quote: ... 0.0005% CHS (10:1) (Anatrace), and 5 mM ATP ...
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bioRxiv - Biochemistry 2020Quote: ... was added before cruel-extraction at 4°C in 1.5% DDM and 0.3% CHS (Anatrace) for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... 5 μg/μL leupeptin and 2% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), mixed using a Dounce homogenizer and incubated for ~16 hr at 4°C with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... The plasma membranes were solubilized at 4 °C with 10x critical micelle concentration (CMC) DDM (Anatrace) in a solution of 20mM HEPES (pH8.0) ...
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bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was exchanged into 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG, Anatrace) or amphipol A8-35 (Anatrace) ...
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bioRxiv - Biophysics 2020Quote: ... 500 μg of OlZAC (500 μl) was mixed with 10 mg of NAPol (Anatrace, dissolved in 100 µl of buffer B) and incubated for 16 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... the 10% DDM stock was supplemented with 2% CHS (Anatrace, Cat#CH210), which resulted in a final lipid:CHS molar ratio of ∼60:40 ...
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bioRxiv - Biophysics 2022Quote: ... 1 mg mL−1 KcsA in 5 mM decyl-β-maltopyranoside (Anatrace) detergent (DM ...
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bioRxiv - Biochemistry 2023Quote: A mixture of 2.75:1.25:1 mg of chloroform-dissolved 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphoethanolamine (POPE): 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphoglycerol (POPG):cholesterol (Anatrace) were dissolved and mixed in chloroform ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or concentrated to 4 mg/mL and vitrified in the presence of 1.5 mM fluorinated fos-choline (FFosC, Anatrace). 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... After resuspension in buffer B (buffer A supplemented with 1% (w/v) n-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.15% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... 0.005 w/v% LMNG-CHS (10:1, Anatrace), and 0.0004 w/v% GDN ...
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bioRxiv - Biochemistry 2022Quote: ... and DDM-CHS (10:1 pre-mix, Anatrace) was then added to a final concentration of 1 % (w/v) ...
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bioRxiv - Biochemistry 2022Quote: ... UBR5 was frozen in GF Buffer with the addition of 4 mM CHAPSO or 0.005 % fluorinated octyl β-maltoside (Anatrace) prior to freezing.
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bioRxiv - Cell Biology 2022Quote: ... at ~5 mg/mL in SEC Buffer containing 2 mM fluorinated fos-choline-8 (Anatrace, cat# F300F), was applied to glow discharged Quantifoil R1.2/1.3 grids ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction was homogenized on ice with Buffer B (20 mL/pellet) containing 1% dodecylphosphocholine (DPC, FOS-CHOLINE-12, Anatrace). Homogenized cells were ultracentrifuged at 100,000 x g for 30 minutes to remove the insoluble fraction ...
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1% sol-grade dodecylmaltoside (DDM, Anatrace), mixed at 4°C for 1 hour and ultracentrifuged ...
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bioRxiv - Biophysics 2022Quote: ... and 0.1 w/v% LMNG-CHS (10:1, Anatrace), pH 7.4 ...