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Citations for anatrace :
251 - 300 of 321 citations for 7 methoxy 4 piperidin 1 ium 1 ylmethyl 3 4 dihydro 2H 1 benzoxepin 5 one chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The lysate was incubated for 1 h at room temperature and then solubilized by 0.5% (w/v) lauryl maltose neopentylglycol (LMNG, Anatrace) supplemented with 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction was pelleted at 100.000 × g and solvated in 1 % (w/v) Dodecyl-malto-pyranoside (DDM, Anatrace). Affinity chromatography with Ni-NTA-Agarose (Peqlab ...
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bioRxiv - Biophysics 2023Quote: ... After resuspension in buffer B (buffer A supplemented with 1% (w/v) n-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.15% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... then solubilised on ice at 4.5 mg mL−1 by the stepwise addition of lauryl maltose neopentyl glycol (LMNG, Anatrace) from a 5% stock solution to a final concentration of 1.5% ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μM Fos-Choline-8 (Anatrace) was added to the protein sample ...
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bioRxiv - Biophysics 2023Quote: ... and 3% deoxy big CHAP (Anatrace) to the same sample and correcting for dilution (Fig ...
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bioRxiv - Plant Biology 2020Quote: ... and were suspended in a 25 mM MES buffer (pH 6.5) containing 1 M betaine at 0.3 mg Chl/mL and solubilized with 1.0% α-DDM (Anatrace, Maumee, OH) for 10 min on ice ...
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bioRxiv - Biophysics 2021Quote: ... with 1 % (v/v) membrane protease inhibitors and 1.0 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM) 40:1 (w/w) CHS (Anatrace). After centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) using a Dounce homogenizer and this membrane suspension was solubilized with 1% dodecyl-maltoside (DDM, Anatrace) and 0.1% cholesteryl hemi-succinate (CHS ...
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bioRxiv - Biophysics 2022Quote: ... The pellet containing the membrane fraction was solubilized in 1% N-dodecyl-β-D-maltopyranoside (DDM) and 0.1% cholesteryl hemisuccinate (CHS; Tris Salt, Anatrace) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the detergent was exchanged with 16 CVs of wash buffer I supplemented with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... equilibrated with the FPLC buffer (20 mM HEPES, pH7.5, 150 mM NaCl) plus 1 mM (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace).
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bioRxiv - Biochemistry 2021Quote: ... and 2 mM MgCl2 were added to the thawed suspension and the mixture was incubated with additional 1% (w/v) dodecyl-β-D-maltopyranoside (DDM; Bluepus) and 0.2% (w/v) cholesteryl hemisuccinate (CHS, Anatrace) at 8°C for 2 h for membrane solubilization and protein extraction ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were solubilized by resuspending in buffer (50 mM Tris-HCl, pH 7.4, 10% [v/v] glycerol, 1% [w/w] dodecyl-β-D-maltoside [DDM, Anatrace] ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were mechanically broken and were resuspended in buffer A (10ml of buffer per gram of membrane) and supplemented with 1% w/v final of dodecyl-maltoside (DDM, Anatrace) for 1h solubilization under gentle stirring ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was lysed in 500 μl of lysis buffer (50 mM Tris-HCl pH 7.2, 10% v/v glycerol, 150 mM NaCl, 1% w/v DIBMA (Anatrace BMA101), with protease inhibitors (1% v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and receptor was subsequently extracted from membranes in a volume of 50 ml by addition of 2× solubilisation buffer (100 mM HEPES pH 7.5, 250 mM NaCl, 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were solubilized by incubating with an addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Immunology 2023Quote: ... The membrane pellet was thoroughly resuspended in TN buffer using a homogenizer and then mixed with a pre-made solution of 10% (w/v) Dodecyl Maltoside (DDM) and 1% (w/v) Cholesteryl Hemisuccinate (CHS) (Anatrace) to a final concentration of 1% DDM and 0.1% CHS ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction was homogenized on ice with Buffer B (20 mL/pellet) containing 1% dodecylphosphocholine (DPC, FOS-CHOLINE-12, Anatrace). Homogenized cells were ultracentrifuged at 100,000 x g for 30 minutes to remove the insoluble fraction ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Giβ1ψ2 G-protein were lysed in hypotonic buffer and G-protein was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Detergent was exchanged from cholate/DDM to DDM on Ni Sepharose column ...
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bioRxiv - Biophysics 2022Quote: ... and the membrane pellet was resuspended in Lysis buffer and the protein was solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.04% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biochemistry 2020Quote: AcrB was extracted from homogenized membranes by overnight incubation with 1 % (w/v) n-dodecyl-β-D-maltoside (DDM) detergent (Anatrace) at 4 °C with gentle agitation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG, Anatrace) at 4 °C for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... The membrane pellets were homogenized in lysis buffer supplemented with 200 mM NaCl and 10% (v/v) glycerol (solubilization buffer) and then incubated with 1% dodecyl-β-D-maltopyranoside (DDM; Anatrace) 0.2% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Molecular Biology 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [HN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Frozen membranes were thawed in a tepid water bath and solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM; Anatrace) with mixing for ∼1 h ...
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bioRxiv - Biophysics 2023Quote: ... which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-Dodecyl-β-D-Maltopyranoside (Anatrace, D310) and 0.2% Cholesteryl Hemisuccinate Tris salt (Anatrace ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The mixture was transferred to 4 °C and allowed to equilibrate for 20 minutes before solubilization was initiated by addition of a final concentration of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), 0.2% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed with the Microfluidizer LM20 (Microfluidics) cell disruptor and lysate was incubated with 1% N-Dodecyl-β-D-maltoside (Anatrace) for 1 1hour at 4°C with rocking ...
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bioRxiv - Biophysics 2023Quote: ... washed once with PBS and were resuspended with 500 μL buffer A containing 1% N-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.1% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM amino acid mixture (average concentration of each amino acid 0.3 mM) and 0.1% (w/v) MNG-3 (Maltose Neopentyl Glycol-3, Anatrace). The feeding buffer contained 1x SUB-AMIX buffer (CellFree Sciences Japan) ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of 8 mg/ml complex with 3 mM CHAPSO or 0.01% fluorinated octyl-maltoside (FOM) (Anatrace) were applied onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... or either 2 mM lyso PC/PG (50:50 mol% of 14:0 lyso PC and 14:0 lyso PG) or 1 mM β-DDM (n-Dodecyl β-D-maltoside) (Anatrace) were added to the samples ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was resuspended again using Buffer W and solubilized by the addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM) (Anatrace, USA), 0.15% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... 3% n-dodecyl-D-maltoside (DDM, Anatrace, OH) was added and the samples were stirred for overnight at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... 3 mM fluorinated Fos-choline 8 (Anatrace, USA) was added to improve particle distribution in vitreous ice ...
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bioRxiv - Molecular Biology 2022Quote: ... 5-6 mM LMNG (Anatrace) and 1-1.2 mM Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biophysics 2022Quote: ... the initial purification for nanodisc incorporation was performed analogously to the GDN prep with the following modifications: Extraction buffer contained 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% Cholesterol Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biochemistry 2019Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 μL of 10% DDM (β-D-dodecyl maltoside, Anatrace) and 13 μL of 27mM mal-PEG (Methoxypolyethylene glycol maleimide ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 μL of GltPh-containing nanodiscs (7 mg/mL) supplemented with 1.5 mM fluorinated Fos-Choline-8 (Anatrace) were applied to a glow-discharged UltrAuFoil R1.2/1.3 300-mesh gold grid (Quantifoil ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 µl of 10% β-d-dodecyl maltoside (Anatrace 329370010) and 13 µl of freshly dissolved 27 mM 5 kDa mal-PEG ...
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bioRxiv - Biophysics 2021Quote: ... purified protein at a concentration of 6–7 mg/mL was mixed with 2.9 mM Fluorinated Fos-Choline-8 (FFC8) (Anatrace) immediately prior to grid preparation ...
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bioRxiv - Biochemistry 2021Quote: ... 1,2-Diheptanoyl-sn-Glycero-3-Phosphocholine (DHPC; Anatrace #D607).
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bioRxiv - Biochemistry 2021Quote: ... 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC; Anatrace #D514); 1,2-Diheptanoyl-sn-Glycero-3-Phosphocholine (DHPC ...
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bioRxiv - Biochemistry 2024Quote: The Doa10 sample was supplemented with 3 mM FFC8 (Anatrace) before freezing cryo-EM grids ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM analytical-grade DM (Anatrace #D32225GM)) ...
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bioRxiv - Biochemistry 2023Quote: ... 5% n-dodecyl-beta-maltoside (DDM) (Anatrace)) and incubated for 1 h at 4C with end-over-end rotation ...
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bioRxiv - Biophysics 2022Quote: ... the membrane fraction was prepared by ultracentrifugation at 100,000 x g for one hour and protein was solubilized by addition of lysis buffer containing 0.5 % LMNG (Anatrace) for 1 h ...