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Citations for anatrace :
51 - 70 of 70 citations for 6 methoxypyridine 3 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Cell powder was added at a ratio of 1 g cell powder to 3 ml lysis buffer (200 mM KCl, 21 mM OGNG (octyl glucose neopentyl glycol, Anatrace), 30 mM HTG (n-heptyl-β-D-thioglucopyranoside ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... 4 mL (1/10 volume) of 1 M n-octyl-β-D-glucopyranoside in H2O (β-OG, Anatrace) was added to 100 mM final concentration ...
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bioRxiv - Microbiology 2024Quote: ... and pellets were dissolved in solubilization buffer (20 mM Tris, 150 NaCl, 15% glycerol, 3% (% w/v) n-dodecyl-β-D-maltoside (DDM; Anatrace)) ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or concentrated to 4 mg/mL and vitrified in the presence of 1.5 mM fluorinated fos-choline (FFosC, Anatrace). 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2022Quote: ... UBR5 was frozen in GF Buffer with the addition of 4 mM CHAPSO or 0.005 % fluorinated octyl β-maltoside (Anatrace) prior to freezing.
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: Native and selenomethionine-labeled LapD2 was concentrated to 10 mg/ml and screened for crystallization conditions using the MCSG1-4 crystallization suites (Anatrace) and the hanging drop vapour diffusion method ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Biochemistry 2022Quote: ... diluted with 4 mL GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.004% [w/v] glyco-diosgenin [GDN; Anatrace], pH 7.4) and concentrated to ~200 μL in the same concentrator ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... at 2.0 mg Chl/mL and solubilized with 4% (w/v) n-dodecyl-α-D-maltoside (α-DDM as previously abbreviated as α-DM; Anatrace) for 30 min with gentle agitation on ice ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biochemistry 2022Quote: ... Drops of 450 nL of 10 mg/mL protein in SEC buffer and 150 nL of MCSG-1 H10 (Anatrace; 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...