Labshake search
Citations for anatrace :
251 - 300 of 309 citations for 6 Quinoxalinamine N 3 8 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... 7[mM NMG (pH 8.0)) and solubilized by adding 40[mM DM (n-Decyl-β-D-Maltoside) (Sol-Grade from Anatrace). KtrB alone ...
-
bioRxiv - Biophysics 2023Quote: ... Protein was extracted via the addition of n-Dodecyl-β-D-maltopyranoside (DDM) with cholesteryl hemisuccinate (CHS) (Anatrace, D310-CH210) to a final concentration of 50 mM DDM / 4 mM CHS and rotated for 1 hour at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... and receptor was subsequently extracted from membranes in a volume of 50 ml by addition of 2× solubilisation buffer (100 mM HEPES pH 7.5, 250 mM NaCl, 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... the membranes were solubilized by incubating with an addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace) and 0.2% (w/v ...
-
Structural insight into guanylyl cyclase receptor hijacking of the kinase–Hsp90 regulatory mechanismbioRxiv - Biochemistry 2023Quote: ... Membranes were thawed and solubilized with the addition of 1% n-dodecyl β-D-maltoside (DDM) and 0.1% cholesteryl hemisuccinate (CHS) (10:1) (Anatrace). Debris and unsolubilized membranes were pelleted by centrifugation at 46,000 x g ...
-
bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
-
bioRxiv - Biophysics 2023Quote: ... the PSII-enriched membranes (0.5 mg Chl/mL) were solubilized with 1.0% (w/v) α-DDM (n-dodecyl-α-D-maltopyranoside, Anatrace) in a buffer containing 25 mM MES-NaOH (pH 6.0 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Giβ1ψ2 G-protein were lysed in hypotonic buffer and G-protein was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Detergent was exchanged from cholate/DDM to DDM on Ni Sepharose column ...
-
bioRxiv - Biochemistry 2021Quote: ... Cell lysate was dounce homogenized (8 strokes with the A pestle) and a 1% Dodecyl maltoside (DDM)/0.2% Cholesteryl hemisuccinate (CHS) (Anatrace) w:w mixture was added for protein solubilization ...
-
bioRxiv - Biochemistry 2021Quote: ... followed by 8 column buffers of GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.006 % (w/v) glyco-diosgenin (GDN, Anatrace), pH 8.0) ...
-
bioRxiv - Biophysics 2022Quote: ... Cryo-EM grids of anaerobically-purified FHL were prepared similarly within the anaerobic chamber except that 0.5x CMC fluorinated fos-choline-8 (Anatrace) was added immediately before the sample was applied to the grid ...
-
bioRxiv - Biochemistry 2024Quote: ... Nanodiscs were formed with a mixture of phospholipids (48% POPC, 32% POPE, 12% DOPS, 8% cardiolipid (w/w)) (Anatrace). The cell-free synthesis protocol was detailed previously [32,36,70] ...
-
bioRxiv - Biochemistry 2020Quote: ... The cells were lysed by cryogenic milling and then solubilized using the detergent n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, solgrade). CST was purified from the clarified lysate using Talon resin (Clontech ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were subsequently incubated for 5 min at 37°C and lysed by the addition of 0.5% N-Dodecyl- ß-D-maltoside (DDM) (Anatrace D310S). Cells were incubated at 37°C for additional 5 to 10 min while monitoring lysis ...
-
bioRxiv - Biophysics 2019Quote: ... together with a surfactant mixture of 1.2 nM of DDM and 1.1 nM n-Octyl-β-D-Glucopyranoside (OG, Anatrace, OH, USA). The protein was incubated for 2 hours before removal of the surfactant ...
-
bioRxiv - Biophysics 2022Quote: ... and the membrane pellet was resuspended in Lysis buffer and the protein was solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.04% cholesteryl hemisuccinate (CHS ...
-
bioRxiv - Biochemistry 2020Quote: AcrB was extracted from homogenized membranes by overnight incubation with 1 % (w/v) n-dodecyl-β-D-maltoside (DDM) detergent (Anatrace) at 4 °C with gentle agitation ...
-
bioRxiv - Cell Biology 2021Quote: ... Two membrane pellets (corresponding to half a fermenter growth) were thawed and solubilized with addition of n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to 1% (w/v ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG, Anatrace) at 4 °C for 30 min ...
-
bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biophysics 2022Quote: ... for 30 min at 4 °C and then solubilized by addition of 2× buffer (50 mM HEPES, 500 mM NaCl, 2 %w/v n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.4 %w/v cholesteryl hemisuccinate (CHS ...
-
bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
-
bioRxiv - Microbiology 2022Quote: ... and extracted with detergent by adding drop-wise a 10% (w/v) stock solution of n-dodecyl β-D-maltoside (DDM, Anatrace) to a final concentration of 1.3% (w/v) ...
-
bioRxiv - Immunology 2020Quote: ... RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological ...
-
bioRxiv - Plant Biology 2022Quote: ... Then the suspension was incubated in the buffer with additional l.5 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) at 4 °C for 2 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [HN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biochemistry 2022Quote: HEK293F cells (for SBA clarified lysate preparation) and HEK293T cells (for expression analysis by Western Blot) were solubilized with n-Dodecyl-β-d-maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
-
bioRxiv - Biophysics 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biochemistry 2023Quote: Frozen membranes were thawed in a tepid water bath and solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM; Anatrace) with mixing for ∼1 h ...
-
bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at room temperature (RT) for 1 hour and the membranes were solubilised by adding 1% (w/v) n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace), and 0.2% (w/v ...
-
bioRxiv - Biophysics 2023Quote: ... which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-Dodecyl-β-D-Maltopyranoside (Anatrace, D310) and 0.2% Cholesteryl Hemisuccinate Tris salt (Anatrace ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The mixture was transferred to 4 °C and allowed to equilibrate for 20 minutes before solubilization was initiated by addition of a final concentration of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), 0.2% (w/v ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were lysed with the Microfluidizer LM20 (Microfluidics) cell disruptor and lysate was incubated with 1% N-Dodecyl-β-D-maltoside (Anatrace) for 1 1hour at 4°C with rocking ...
-
bioRxiv - Biophysics 2023Quote: ... washed once with PBS and were resuspended with 500 μL buffer A containing 1% N-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.1% cholesteryl hemisuccinate (CHS ...
-
bioRxiv - Biophysics 2024Quote: ... 2.7 µL of sample was mixed with 0.3 µL of fluorinated fos-choline-8 solution (Anatrace; final concentration 1.5 mM) and applied to UltrAuFoil 0.6/1 300 mesh gold grids (Quantifoil) ...
-
bioRxiv - Plant Biology 2022Quote: ... Membranes were solubilized by addition of an equal volume of ACA 750 containing 2% n-dodecyl β-D-maltoside (β-DM, Anatrace) for a final concentration of 0.5 mg mL−1 chlorophyll and 1% β-DM ...
-
bioRxiv - Biochemistry 2021Quote: ... and 5 mM BME and further solubilized by stirring with the addition of 1% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, OH) for an hour at 4 °C ...
-
bioRxiv - Biophysics 2021Quote: ... proteins were overexpressed in BL21-AI E.coli competent cells and extracted into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris(2-carboxyethyl)phosphine ...
-
bioRxiv - Biochemistry 2022Quote: ... or either 2 mM lyso PC/PG (50:50 mol% of 14:0 lyso PC and 14:0 lyso PG) or 1 mM β-DDM (n-Dodecyl β-D-maltoside) (Anatrace) were added to the samples ...
-
bioRxiv - Biophysics 2022Quote: ... The membrane was resuspended again using Buffer W and solubilized by the addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM) (Anatrace, USA), 0.15% (w/v ...
-
bioRxiv - Biophysics 2024Quote: ... N-dodecyl-β-D-maltoside (DDM) and Anapoe® X-100 (Triton® X-100) were purchased from Anatrace (Maumee, OH, USA). 50 mesh BioBeads® were purchased from Bio-Rad (Hercules ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by 8 column buffers of GTBS (50 mM Tris HCl, 150 mM NaCl, 0.006% [w/v] glyco-diosgenin [GDN; Anatrace], pH 8.0). Ycf1p was eluted with 6 column volumes of GTBS containing 150 μg/mL 3×FLAG peptide ...
-
bioRxiv - Immunology 2023Quote: ... the complex was extracted from the membrane with 0.8% (w/v) lauryl maltose neopentylglycol (LMNG; Anatrace, CAS. No. 1423315-02-8) and 0.1% (w/v ...
-
bioRxiv - Microbiology 2020Quote: ... 20 mL of solubilization solution supplemented with the 2 % 6-Cyclohexyl-1-Hexyl-β-D-Maltoside (Cymal6, Anatrace) was added gently to the membrane solution and the sample incubated at RT for 30 min under moderate shaking ...
-
bioRxiv - Neuroscience 2021Quote: ... The supernatant was discarded and membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
-
bioRxiv - Biophysics 2022Quote: ... Membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM HEPES, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% cholesteryl hemisuccinate Tris salt (CHS ...
-
bioRxiv - Plant Biology 2020Quote: ... at 2.0 mg Chl/mL and solubilized with 4% (w/v) n-dodecyl-α-D-maltoside (α-DDM as previously abbreviated as α-DM; Anatrace) for 30 min with gentle agitation on ice ...
-
bioRxiv - Biophysics 2021Quote: ... Membrane pellets were scooped into a dounce homogenizer containing Extraction Buffer (50 mM Tris, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
-
bioRxiv - Biophysics 2022Quote: ... the initial purification for nanodisc incorporation was performed analogously to the GDN prep with the following modifications: Extraction buffer contained 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% Cholesterol Hemisuccinate Tris Salt (CHS ...
-
bioRxiv - Biophysics 2023Quote: ... coli cells transformed with the expression plasmid were lysed by sonication and the protein extracted from membrane fragments into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris (2-carboxyethyl ...