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Citations for anatrace :
51 - 100 of 104 citations for 6 Fluoro 2 4 dihydroxyquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The membrane pellets were solubilized in 2% lauryl maltose neopentyl glycol (LMNG, Anatrace) in the membrane solubilization buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... Detergent was added to the homogenate (final 2% octyl β-D-glucopyranoside, Anatrace) and solubilized overnight with rocking at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Detergent was added to the homogenate (final 2% octyl ß-D-glucopyranoside, Anatrace) and solubilized overnight with rocking at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... 5 μg/μL leupeptin and 2% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), mixed using a Dounce homogenizer and incubated for ~16 hr at 4°C with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Expressed protein was solubilized in 2 % lauryl maltose neopentyl glycol (LMNG) (Anatrace, NG310), 0.2 % Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... 4 mL (1/10 volume) of 1 M n-octyl-β-D-glucopyranoside in H2O (β-OG, Anatrace) was added to 100 mM final concentration ...
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bioRxiv - Biophysics 2020Quote: ... 2% (w/v) n-Dodecyl-β-D-maltopyranoside (DDM; Anatrace, Maumee, OH, USA; #D310), 1% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1 mM PMSF and 2% w/v lauryl maltose neopentyl glycol (L-MNG, Anatrace) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: 2.5mg of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) in chloroform (Anatrace) were dried for 3h under argon flow ...
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bioRxiv - Biophysics 2022Quote: ... rat cerebellar membranes were solubilized in 2 mM Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.1% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... 2 mM pepstatin A (which are all protease inhibitors) plus 1% GDN detergent (Anatrace). Protein was extracted from the membrane by whole-cell solubilization for 1 h at 4 °C with rotation ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or concentrated to 4 mg/mL and vitrified in the presence of 1.5 mM fluorinated fos-choline (FFosC, Anatrace). 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was solubilized in 2% (w/v) n-Dodecyl β-D-maltoside (DDM, Anatrace) for 2 hours while stirring ...
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bioRxiv - Biophysics 2023Quote: ... Protein extraction was carried out with 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace #D322S25GM) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... protein was extracted with 2% (w/v) decyl-ý-D-maltopyranoside (DM; Anatrace, Maumee, OH) for 2 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... UBR5 was frozen in GF Buffer with the addition of 4 mM CHAPSO or 0.005 % fluorinated octyl β-maltoside (Anatrace) prior to freezing.
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: Native and selenomethionine-labeled LapD2 was concentrated to 10 mg/ml and screened for crystallization conditions using the MCSG1-4 crystallization suites (Anatrace) and the hanging drop vapour diffusion method ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Biochemistry 2020Quote: TRPC4 membranes were solubilized for 2 hr in buffer B supplemented with 1% LMNG (Anatrace #NG310). Then a protocol similar to that used for DDM purification was followed ...
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bioRxiv - Biochemistry 2020Quote: Membranes were solubilized for 2 hr in buffer B supplemented with 1% dodecyl maltoside (Anatrace #D310). Insoluble material was removed by ultracentrifugation for 1 hr in a Beckman Coulter Type 70 Ti rotor at 40,000 rpm ...
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bioRxiv - Biochemistry 2019Quote: ... then treated with 1 uL of sodium 2-sulfonatoethyl methanethiosulfonate (MTSES, Anatrace, 10x stock in water) to a working concentration of 100 uM ...
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bioRxiv - Molecular Biology 2023Quote: ... Washed membranes were resuspended in solubilisation buffer (25 mM PIPES, 300 mM NaCl, 2% CHAPSO (Anatrace), 5% glycerol ...
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bioRxiv - Biochemistry 2023Quote: ... Infected Hi5 cells were collected at 72 hr post infection and lysed in 2% CHAPSO (Anatrace) buffer (25 mM Pipes pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Biochemistry 2022Quote: ... diluted with 4 mL GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.004% [w/v] glyco-diosgenin [GDN; Anatrace], pH 7.4) and concentrated to ~200 μL in the same concentrator ...
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bioRxiv - Biophysics 2021Quote: ... then incubated for 1 hour in the same resuspension buffer supplemented with 2 % DDM (Anatrace, OH, USA) and 0.1 mg/ml cardiolipin ...
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bioRxiv - Biochemistry 2022Quote: ... Resuspended pellets were lysed by sonication and extracted with 2% n-Decyl-β-D-Maltopyranoside (DM) (Anatrace) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... at ~5 mg/mL in SEC Buffer containing 2 mM fluorinated fos-choline-8 (Anatrace, cat# F300F), was applied to glow discharged Quantifoil R1.2/1.3 grids ...
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bioRxiv - Biochemistry 2023Quote: ... The dried film was then resuspended in buffered water with 2% (w/v) β-n-dodecylmaltoside (Anatrace) and sonicated (Branson sonifier ...
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bioRxiv - Biophysics 2019Quote: ... A column was packed with the Sepharose beads and washed with Wash Buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 2 mM TCEP) containing 0.006% DMNG (Anatrace). TRPV2 was eluted with Wash Buffer containing 0.006% DMNG and 3 mg/ml 1D4 peptide (GenScript USA ...
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bioRxiv - Biophysics 2020Quote: ... The mutant CLC-ec1 proteins were extracted with n-decyl-β-D-maltopyranoside (DM, Anatrace; 2% (w/v)) and allowed to bind to cobalt resin (Talon ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of 0.6 mg/mL LayV G or 2 mg/ml LayV G with 0.01% fluorinated octyl-maltoside (Anatrace) detergent were applied onto a freshly glow discharged 2.0/2.0 UltraFoil79 grid (200 mesh) ...
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bioRxiv - Plant Biology 2020Quote: ... at 2.0 mg Chl/mL and solubilized with 4% (w/v) n-dodecyl-α-D-maltoside (α-DDM as previously abbreviated as α-DM; Anatrace) for 30 min with gentle agitation on ice ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biochemistry 2019Quote: ... and then resuspended to 100 μl with solubilization buffer (85 mM NaCl, 17 mM Tris, pH 7.5 and 2% sodium dodecanoyl sarcosine (Anatrace)) ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) using a Dounce homogenizer and this membrane suspension was solubilized with 1% dodecyl-maltoside (DDM, Anatrace) and 0.1% cholesteryl hemi-succinate (CHS ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction pellets were solubilized in solubilization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2% n-dodecyl-beta-d-maltopyranoside (DDM) (Anatrace, USA) with 1 mM PMSF ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane pellets were solubilized in 1 ml lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol, protease inhibitor cocktail, 2% UDM (Anatrace U300LA) on a rotary shaker for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... 1,2-Dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) and 3((3-cholamidopropyl)- dimethylammonio)-2-hydroxy-1-propanesulphonate (CHAPSO) were purchased from Anatrace. Sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were solubilized by douncing in solubilization buffer (20 mM HEPES pH 8.0, 300 mM NaCl, 2% DDM detergent (Anatrace D310S), 50 mM glutamate ...
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bioRxiv - Biophysics 2020Quote: ... 10 μg of OlZAC (10 μl) was mixed with 200 μg of NAPol (Anatrace, dissolved in 2 µl of buffer B) and incubated for 16 hours ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace, Maumee, OH) and incubating for 1 h at 4° C ...