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Citations for anatrace :
251 - 300 of 477 citations for 6 Chloro 2 4 chlorophenyl N N dimethylimidazo 1 2 α pyridine 3 methanamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... protein sample was spun down at 290,000 x g for 20 min and mixed with either n-octyl-β-D-glucopyranoside (OG; Anatrace) or n-nonyl-β-D-glucoside (NG ...
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bioRxiv - Biochemistry 2020Quote: ... with or without 1.5 mg/ml cardiolipin was prepared in water and solubilized using 8% (w/vol) n-octyl-β-D-maltopyranoside (Anatrace). Protein that had been purified by SEC in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: A total of 4.0 μl of 20 μM TpCorC protein in 20 mM HEPES (pH 7.5) containing 0.03% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, USA) and either 150 mM NaCl or 150 mM KCl was mixed with 0.5 μl of 2 mM EDTA ...
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding 50 mM n-Dodecyl-β-D-maltopyranoside with 0.25% cholesteryl hemisuccinate (Anatrace, D310-CH210) and nutating for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Cells membranes were isolated by ultracentrifugation at 200,000 x g (45 min) and solubilised with 40 mM n-dodecyl-β-D-maltopyranoside (DDM, Anatrace). Solubilised membranes were incubated with Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... the transfected 293T cells were harvested and lysed in PBS buffer containing 0.3 % n-decyl-β-D-maltopyranoside (DDM, Anatrace). Cell lysates were incubated with Protein A/G PLUS Agarose (Santa Cruz ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was incubated for 1 h at room temperature and the complex was solubilized from the membrane using 0.5% (w/v) n-dodecyl-β-d-maltoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... 7[mM NMG (pH 8.0)) and solubilized by adding 40[mM DM (n-Decyl-β-D-Maltoside) (Sol-Grade from Anatrace). KtrB alone ...
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bioRxiv - Biophysics 2023Quote: ... Protein was extracted via the addition of n-Dodecyl-β-D-maltopyranoside (DDM) with cholesteryl hemisuccinate (CHS) (Anatrace, D310-CH210) to a final concentration of 50 mM DDM / 4 mM CHS and rotated for 1 hour at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... 2 mM pepstatin A (which are all protease inhibitors) plus 1% GDN detergent (Anatrace). Protein was extracted from the membrane by whole-cell solubilization for 1 h at 4 °C with rotation ...
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bioRxiv - Microbiology 2019Quote: ... 0.6% 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS, Anatrace), 0.5% Triton X-100 reduced ...
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bioRxiv - Biophysics 2021Quote: ... and 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS, Anatrace) and purified by Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... 2-Aminoethyl methane thiosulfonate hydrobromide (MTSEA) and [2-(trimethylammonium)ethyl] methane thiosulfonate bromide (MTSET) were purchased from Anatrace. CHIBA-3007 and desmethyl-CHIBA-3007 were generous gifts of Dr ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were lysed by cryogenic milling and then solubilized using the detergent n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, solgrade). CST was purified from the clarified lysate using Talon resin (Clontech ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently incubated for 5 min at 37°C and lysed by the addition of 0.5% N-Dodecyl- ß-D-maltoside (DDM) (Anatrace D310S). Cells were incubated at 37°C for additional 5 to 10 min while monitoring lysis ...
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bioRxiv - Biophysics 2019Quote: ... together with a surfactant mixture of 1.2 nM of DDM and 1.1 nM n-Octyl-β-D-Glucopyranoside (OG, Anatrace, OH, USA). The protein was incubated for 2 hours before removal of the surfactant ...
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bioRxiv - Cell Biology 2021Quote: ... Two membrane pellets (corresponding to half a fermenter growth) were thawed and solubilized with addition of n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to 1% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... and extracted with detergent by adding drop-wise a 10% (w/v) stock solution of n-dodecyl β-D-maltoside (DDM, Anatrace) to a final concentration of 1.3% (w/v) ...
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bioRxiv - Immunology 2020Quote: ... RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological ...
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bioRxiv - Plant Biology 2022Quote: ... Then the suspension was incubated in the buffer with additional l.5 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells (for SBA clarified lysate preparation) and HEK293T cells (for expression analysis by Western Blot) were solubilized with n-Dodecyl-β-d-maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
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bioRxiv - Neuroscience 2021Quote: ... 2% (wt/vol final concentration) digitonin (Anatrace) powder was directly dissolved in the cell resuspension ...
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bioRxiv - Molecular Biology 2023Quote: ... 2% Lauryl Maltose Neopentyl Glycol (LMNG; Anatrace), supplemented with EDTA-free protease inhibitor at 4°C for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... N-dodecyl-β-D-maltoside (DDM) and Anapoe® X-100 (Triton® X-100) were purchased from Anatrace (Maumee, OH, USA). 50 mesh BioBeads® were purchased from Bio-Rad (Hercules ...
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bioRxiv - Biochemistry 2020Quote: TRPC4 membranes were solubilized for 2 hr in buffer B supplemented with 1% LMNG (Anatrace #NG310). Then a protocol similar to that used for DDM purification was followed ...
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bioRxiv - Biochemistry 2020Quote: Membranes were solubilized for 2 hr in buffer B supplemented with 1% dodecyl maltoside (Anatrace #D310). Insoluble material was removed by ultracentrifugation for 1 hr in a Beckman Coulter Type 70 Ti rotor at 40,000 rpm ...
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bioRxiv - Biochemistry 2019Quote: ... then treated with 1 uL of sodium 2-sulfonatoethyl methanethiosulfonate (MTSES, Anatrace, 10x stock in water) to a working concentration of 100 uM ...
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bioRxiv - Biophysics 2021Quote: ... then incubated for 1 hour in the same resuspension buffer supplemented with 2 % DDM (Anatrace, OH, USA) and 0.1 mg/ml cardiolipin ...
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bioRxiv - Biophysics 2022Quote: ... Membrane proteins were solubilized from 2.4 L of pelleted HEK293S GnTI-cells expressing wild-type hIP3R3 for 2 hours by rotation in 2% lauryl maltose neopentyl glycol (LMNG; Anatrace Cat# NG310), 150 mM sodium chloride (NaCl) ...
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bioRxiv - Biochemistry 2022Quote: ... 2% (w/v) β-dodecyl maltoside (β-DDM, Anatrace), 0.5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... 2% w/v lauryl maltose neopentyl glycol (LMNG; Anatrace) and 0.2% w/v cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biophysics 2019Quote: ... 35 mM of 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS (Anatrace, Maumee OH)) detergent was added and the solution was sonicated in a cylindrical bath sonicator for 30–60 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1:3 (w/w) PmalC8 (Anatrace) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 200 mM NaCl, 2 mM MgAc2, 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche], 1% [w/v] glyco-diosgenin [GDN; Anatrace], 1 mM DTT) at a ratio 6.8 ml solubilization buffer per 1 g cell pellet ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the GLUT3 protein was purified in 0.06% (w/v) 6-cyclohexyl-1-hexyl-ß-D-maltoside (Cymal-6, Anatrace) and concentrated to ~ 40 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... Initial crystals formed in the MCSG-2 crystallization screen (Anatrace) and optimized crystals were grown in 100 mM sodium acetate ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) using a Dounce homogenizer and this membrane suspension was solubilized with 1% dodecyl-maltoside (DDM, Anatrace) and 0.1% cholesteryl hemi-succinate (CHS ...
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bioRxiv - Biochemistry 2023Quote: ... Destabilization of liposomes was incubated with 2% (wt/vol) DDM (Anatrace) as a final concentration at 25 °C for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... with 2 × CMC (critical micelle concentration) of the LDAO (Anatrace D360) detergent using a 7 kDa Zeba spin desalting columns (Thermo) ...
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bioRxiv - Biochemistry 2020Quote: Membrane was solubilized by addition of 2% final concentration of LMNG (Anatrace) and stirring for 1.5 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... the 10% DDM stock was supplemented with 2% CHS (Anatrace, Cat#CH210), which resulted in a final lipid:CHS molar ratio of ∼60:40 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cymal-6 (Anatrace) was added to the Env(- ...
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bioRxiv - Biophysics 2020Quote: ... The membrane pellets were solubilized in 2% lauryl maltose neopentyl glycol (LMNG, Anatrace) in the membrane solubilization buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... Detergent was added to the homogenate (final 2% octyl β-D-glucopyranoside, Anatrace) and solubilized overnight with rocking at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Detergent was added to the homogenate (final 2% octyl ß-D-glucopyranoside, Anatrace) and solubilized overnight with rocking at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Expressed protein was solubilized in 2 % lauryl maltose neopentyl glycol (LMNG) (Anatrace, NG310), 0.2 % Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biochemistry 2024Quote: 3.5 uL of nhTMEM16-containing nanodiscs (3-7mg ml-1) supplemented with 1.5 or 3 mM Fos-Choline-8-Fluorinated (Anatrace) was applied to a glow-discharged UltrAuFoil R1.2/1.3 300-mesh gold grid (Quantifoil ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...