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Citations for anatrace :
1 - 50 of 282 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: The pelleted membrane fractions were then solubilized in resuspension buffer (50 mM Tris/Mes, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5) containing 1% β-DDM (Anatrace) and stirred on ice for 60minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... + 0.1% Tween 20 (Anatrace)) and then probed with primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... at 0.1-0.3mg/mL in EM buffer containing either 0.02% Tween-20 or 0.025% amphipol A8-35 (Anatrace) was applied to Quantifoil or UltrAufoil R1.2/1.3 grids (Quantifoil ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of solubilization solution supplemented with the 2 % 6-Cyclohexyl-1-Hexyl-β-D-Maltoside (Cymal6, Anatrace) was added gently to the membrane solution and the sample incubated at RT for 30 min under moderate shaking ...
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bioRxiv - Biophysics 2022Quote: ... membrane pellets were homogenized in TBS (20 mM Tris-HCl, pH 7.5, 150 mM NaCl) and solubilized in TBS with 1% GDN (Anatrace), supplemented with protease inhibitors (1 mg/ml Leupeptin ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biochemistry 2022Quote: ... equilibrated with the FPLC buffer (20 mM HEPES, pH7.5, 150 mM NaCl) plus 1 mM (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace).
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction was homogenized on ice with Buffer B (20 mL/pellet) containing 1% dodecylphosphocholine (DPC, FOS-CHOLINE-12, Anatrace). Homogenized cells were ultracentrifuged at 100,000 x g for 30 minutes to remove the insoluble fraction ...
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bioRxiv - Biophysics 2023Quote: ... which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-Dodecyl-β-D-Maltopyranoside (Anatrace, D310) and 0.2% Cholesteryl Hemisuccinate Tris salt (Anatrace ...
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bioRxiv - Biophysics 2021Quote: A total of 4.0 μl of 20 μM TpCorC protein in 20 mM HEPES (pH 7.5) containing 0.03% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, USA) and either 150 mM NaCl or 150 mM KCl was mixed with 0.5 μl of 2 mM EDTA ...
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bioRxiv - Biophysics 2021Quote: ... 20 CV WB with 0.06 % (w/v) glyco-diosgenin (GDN) (Anatrace) and 20 CV WB with 0.02 % (w/v ...
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bioRxiv - Biophysics 2023Quote: ... 20 mM imidazole and 85 μM glyco-diosgenin (GDN, GDN101, Anatrace). The protein was eluted with 200 mM imidazole and digested with PreScission protease at 4 ℃ overnight to remove the C-terminal GFP-His10 tag ...
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bioRxiv - Biophysics 2019Quote: Membrane pellets were solubilized in resuspension buffer containing 20 mM β-DDM (Anatrace) and 0.5 mM DTT and incubated on ice with stirring for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... and washed with 20 CV of buffer A supplemented with 0.02% (w/v) GDN (Anatrace). The bound proteins of ERGIC-53 WT were eluted with overnight incubation of 2 CV elution buffer (buffer A supplemented with 0.02% GDN ...
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bioRxiv - Biophysics 2019Quote: ... A column was packed with the Sepharose beads and washed with Wash Buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 2 mM TCEP) containing 0.006% DMNG (Anatrace). TRPV2 was eluted with Wash Buffer containing 0.006% DMNG and 3 mg/ml 1D4 peptide (GenScript USA ...
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bioRxiv - Biochemistry 2020Quote: ... The protein-bound resin was washed with 10 mM imidazole and eluted with imidazole (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 20 % glycerol, 0.1% DDM (Anatrace, USA), 1 M imidazole) ...
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bioRxiv - Biophysics 2023Quote: ... and the cell pellets were resuspended in lysis buffer (50 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 5 mM MgCl2, 20 mM imidazole, 1mM CHAPS (Anatrace), 5mM 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... Resin was loaded onto a column and washed with wash buffer (20 mM HEPES pH 8.0, 300 mM NaCl, 0.05 % DDM (Anatrace D310), 50 mM glutamate ...
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bioRxiv - Neuroscience 2023Quote: ... on a Superose 6 column in SEC buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 0.05 % DDM detergent (Anatrace D310), and 50 mM glutamate).
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bioRxiv - Biophysics 2023Quote: ... and the resin was washed with 20 column volumes of GDN buffer (150 mM NaCl, 20 mM Tris pH 8.0, 0.01% GDN (glycol-diosgenin, Anatrace, GDN101)) ...
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bioRxiv - Biochemistry 2020Quote: ... protein sample was spun down at 290,000 x g for 20 min and mixed with either n-octyl-β-D-glucopyranoside (OG; Anatrace) or n-nonyl-β-D-glucoside (NG ...
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bioRxiv - Biophysics 2023Quote: ... the lipid mixture was solubilized in 500 μL of 20 mM HEPES buffer (pH 7.5, 5%-w/v β-octylglucoside (Anatrace)) at the final lipid concentration of 7.5%-w/v ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane pellets were solubilized in 1 ml lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol, protease inhibitor cocktail, 2% UDM (Anatrace U300LA) on a rotary shaker for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were solubilized by douncing in solubilization buffer (20 mM HEPES pH 8.0, 300 mM NaCl, 2% DDM detergent (Anatrace D310S), 50 mM glutamate ...
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bioRxiv - Microbiology 2024Quote: ... and pellets were dissolved in solubilization buffer (20 mM Tris, 150 NaCl, 15% glycerol, 3% (% w/v) n-dodecyl-β-D-maltoside (DDM; Anatrace)) ...
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bioRxiv - Biochemistry 2023Quote: ... and PACT kits (Molecular Dimensions, Anatrace, Inc.) at 20 ℃ ...
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bioRxiv - Biochemistry 2021Quote: ... 1% DMNG (Anatrace) for 1hr ...
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bioRxiv - Biophysics 2022Quote: ... 1 mg mL−1 KcsA in 5 mM decyl-β-maltopyranoside (Anatrace) detergent (DM ...
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bioRxiv - Biophysics 2020Quote: ... 1% (w/v) 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS; Anatrace; #C216), and 0.2% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... 1% (w/v) DDM and 1% (w/v) β-D-Ocytlglucoside (Anatrace). Sample aliquots of 100 μl were heated for 10 min over a temperature range of 20–80°C in a PCR thermocycler (Veriti ...
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bioRxiv - Biophysics 2019Quote: ... and 1% C12E8 (Anatrace) for 2 hours at 4°C and ultracentrifuged at 186,000g for 1 hour at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM phenylmethanesulfonyl fluoride (PMSF) and 1% (w/v) glyco-diosgenin (GDN, Anatrace) at 4 °C for 80 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% (w/v) GDN (Anatrace), 2 mM PMSF ...
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bioRxiv - Biochemistry 2021Quote: ... 1% (w/v) (DDM (Anatrace) and 0.2% (w/v ...
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Structural insight into guanylyl cyclase receptor hijacking of the kinase–Hsp90 regulatory mechanismbioRxiv - Biochemistry 2023Quote: ... 0.0005% CHS (10:1) (Anatrace), and 5 mM ATP ...
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bioRxiv - Biophysics 2024Quote: ... 1 mM dodecylmaltoside (Anatrace, anagrade) and 0.1 mg/ml lipid mixture (3:1:1 of POPC:POPE:POPG) ...
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bioRxiv - Biochemistry 2024Quote: ... crystal conditions screened using the MCSG2 crystallization kit (Anatrace Products). X-ray quality crystals grew after 24 hours at 4 ℃ using the condition ...
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bioRxiv - Biochemistry 2021Quote: ... Diluted membranes were solubilized by the addition of 1 % of OGNG (or 1 % DDM) (Anatrace) in the presence of 1 mM PMSF and some crystals of PEFA-Bloc for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% (wt/vol) Cymal-5 (Anatrace), and a cocktail of protease inhibitors (Roche Complete EDTA-free tablets) ...
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bioRxiv - Biochemistry 2022Quote: ... DDM/CHS detergent (1%/0.1%) (Anatrace) was added to lyse the cells for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in 1% DMNG (Anatrace) lysis buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM EDTA) and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, Inc. Maumee, OH), EDTA-free complete protease inhibitor cocktail (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM EDTA) and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, Inc. Maumee, OH), EDTA-free complete protease inhibitor cocktail (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM EDTA) and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, Inc. Maumee, OH), EDTA-free complete protease inhibitor cocktail (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM EDTA) and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, Inc. Maumee, OH), EDTA-free complete protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2022Quote: ... 1 mM EDTA) and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, Inc. Maumee, OH), EDTA-free complete protease inhibitor cocktail (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM EDTA) and 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, Inc. Maumee, OH), EDTA-free complete protease inhibitor cocktail (Roche ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... BtuG1 was screened for crystals using the MCSG1 crystallization kit (Anatrace Products). Several hits were observed after 24 hours at 4℃ ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... 4 mL (1/10 volume) of 1 M n-octyl-β-D-glucopyranoside in H2O (β-OG, Anatrace) was added to 100 mM final concentration ...